Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4786 and Pf1N1B4_4787 are separated by 165 nucleotides Pf1N1B4_4787 and Pf1N1B4_4788 are separated by 14 nucleotides Pf1N1B4_4788 and Pf1N1B4_4789 are separated by 136 nucleotides
Pf1N1B4_4786 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 5,288,286 to 5,289,479
_4786
Pf1N1B4_4787 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 5,289,645 to 5,290,772
_4787
Pf1N1B4_4788 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 5,290,787 to 5,291,560
_4788
Pf1N1B4_4789 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 5,291,697 to 5,292,848
_4789
Position (kb)
5289
5290
5291 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5289.036 kb on - strand at 5289.474 kb on + strand at 5289.474 kb on + strand at 5289.617 kb on + strand at 5289.761 kb on + strand at 5289.762 kb on - strand, within Pf1N1B4_4787 at 5289.917 kb on - strand, within Pf1N1B4_4787 at 5290.080 kb on + strand, within Pf1N1B4_4787 at 5290.080 kb on + strand, within Pf1N1B4_4787 at 5290.215 kb on + strand, within Pf1N1B4_4787 at 5290.218 kb on - strand, within Pf1N1B4_4787 at 5290.218 kb on - strand at 5290.262 kb on + strand, within Pf1N1B4_4787 at 5290.539 kb on + strand, within Pf1N1B4_4787 at 5290.566 kb on - strand, within Pf1N1B4_4787 at 5290.722 kb on + strand at 5290.723 kb on - strand at 5290.723 kb on - strand at 5290.723 kb on - strand at 5290.786 kb on + strand at 5290.786 kb on + strand at 5290.787 kb on - strand at 5290.787 kb on - strand at 5290.787 kb on - strand at 5290.787 kb on - strand at 5290.794 kb on - strand at 5291.021 kb on + strand, within Pf1N1B4_4788 at 5291.022 kb on - strand, within Pf1N1B4_4788 at 5291.525 kb on - strand at 5291.558 kb on + strand at 5291.558 kb on + strand at 5291.708 kb on - strand at 5291.708 kb on - strand at 5291.743 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 5,289,036 - +1.3 5,289,474 + -1.1 5,289,474 + +0.2 5,289,617 + +1.7 5,289,761 + -0.2 5,289,762 - Pf1N1B4_4787 0.10 +0.3 5,289,917 - Pf1N1B4_4787 0.24 -1.0 5,290,080 + Pf1N1B4_4787 0.39 -2.0 5,290,080 + Pf1N1B4_4787 0.39 -1.2 5,290,215 + Pf1N1B4_4787 0.51 +0.0 5,290,218 - Pf1N1B4_4787 0.51 +1.3 5,290,218 - +0.9 5,290,262 + Pf1N1B4_4787 0.55 -0.8 5,290,539 + Pf1N1B4_4787 0.79 -2.2 5,290,566 - Pf1N1B4_4787 0.82 -0.9 5,290,722 + -0.2 5,290,723 - +1.0 5,290,723 - +1.1 5,290,723 - -0.7 5,290,786 + +0.8 5,290,786 + +0.3 5,290,787 - +0.0 5,290,787 - +0.0 5,290,787 - +0.3 5,290,787 - +1.0 5,290,794 - +0.3 5,291,021 + Pf1N1B4_4788 0.30 +0.3 5,291,022 - Pf1N1B4_4788 0.30 -1.0 5,291,525 - -0.7 5,291,558 + +1.8 5,291,558 + -0.3 5,291,708 - +0.3 5,291,708 - +1.9 5,291,743 - +2.3
Or see this region's nucleotide sequence