Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_475 and Pf1N1B4_476 overlap by 4 nucleotides Pf1N1B4_476 and Pf1N1B4_477 are separated by 132 nucleotides Pf1N1B4_477 and Pf1N1B4_478 are separated by 91 nucleotides
Pf1N1B4_475 - ABC-type multidrug transport system, permease component, at 567,254 to 568,033
_475
Pf1N1B4_476 - ABC-type multidrug transport system, ATPase component, at 568,030 to 568,962
_476
Pf1N1B4_477 - Uncharacterized glutathione S-transferase-like protein, at 569,095 to 569,670
_477
Pf1N1B4_478 - Transglutaminase-like enzymes, putative cysteine proteases, at 569,762 to 570,421
_478
Position (kb) 568
569 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 567.111 kb on + strand at 567.111 kb on + strand at 567.151 kb on + strand at 567.152 kb on - strand at 567.152 kb on - strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.155 kb on + strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.156 kb on - strand at 567.213 kb on - strand at 567.213 kb on - strand at 567.224 kb on - strand at 567.250 kb on + strand at 567.251 kb on - strand at 567.303 kb on + strand at 567.303 kb on + strand at 567.303 kb on + strand at 567.303 kb on + strand at 567.304 kb on - strand at 567.304 kb on - strand at 567.304 kb on - strand at 567.304 kb on - strand at 567.304 kb on - strand at 567.304 kb on - strand at 567.304 kb on - strand at 567.304 kb on - strand at 567.304 kb on - strand at 567.304 kb on - strand at 567.304 kb on - strand at 567.321 kb on - strand at 567.460 kb on - strand, within Pf1N1B4_475 at 567.518 kb on - strand, within Pf1N1B4_475 at 567.700 kb on - strand at 567.798 kb on + strand, within Pf1N1B4_475 at 567.843 kb on + strand, within Pf1N1B4_475 at 567.843 kb on + strand, within Pf1N1B4_475 at 567.844 kb on - strand, within Pf1N1B4_475 at 567.852 kb on + strand at 567.852 kb on + strand, within Pf1N1B4_475 at 567.852 kb on + strand, within Pf1N1B4_475 at 567.853 kb on - strand, within Pf1N1B4_475 at 567.853 kb on - strand, within Pf1N1B4_475 at 567.912 kb on + strand, within Pf1N1B4_475 at 567.913 kb on - strand, within Pf1N1B4_475 at 567.995 kb on - strand at 568.012 kb on + strand at 568.012 kb on + strand at 568.012 kb on + strand at 568.013 kb on - strand at 568.156 kb on - strand, within Pf1N1B4_476 at 568.191 kb on + strand, within Pf1N1B4_476 at 568.605 kb on - strand, within Pf1N1B4_476 at 568.646 kb on + strand, within Pf1N1B4_476 at 568.649 kb on + strand, within Pf1N1B4_476 at 568.650 kb on - strand, within Pf1N1B4_476 at 568.773 kb on + strand, within Pf1N1B4_476 at 568.773 kb on + strand, within Pf1N1B4_476 at 568.774 kb on - strand at 568.996 kb on + strand at 568.997 kb on - strand at 568.997 kb on - strand at 569.000 kb on + strand at 569.000 kb on + strand at 569.001 kb on - strand at 569.001 kb on - strand at 569.001 kb on - strand at 569.020 kb on + strand at 569.020 kb on + strand at 569.020 kb on + strand at 569.020 kb on + strand at 569.021 kb on - strand at 569.068 kb on + strand at 569.068 kb on + strand at 569.068 kb on + strand at 569.069 kb on - strand at 569.069 kb on - strand at 569.069 kb on - strand at 569.069 kb on - strand at 569.094 kb on - strand at 569.094 kb on - strand at 569.096 kb on + strand at 569.096 kb on + strand at 569.096 kb on + strand at 569.097 kb on - strand at 569.097 kb on - strand at 569.097 kb on - strand at 569.097 kb on - strand at 569.097 kb on - strand at 569.097 kb on - strand at 569.097 kb on - strand at 569.097 kb on - strand at 569.097 kb on - strand at 569.097 kb on - strand at 569.151 kb on + strand at 569.152 kb on - strand at 569.153 kb on + strand, within Pf1N1B4_477 at 569.153 kb on + strand, within Pf1N1B4_477 at 569.154 kb on - strand, within Pf1N1B4_477 at 569.154 kb on - strand, within Pf1N1B4_477 at 569.210 kb on + strand, within Pf1N1B4_477 at 569.210 kb on + strand, within Pf1N1B4_477 at 569.210 kb on + strand, within Pf1N1B4_477 at 569.210 kb on + strand at 569.211 kb on - strand, within Pf1N1B4_477 at 569.211 kb on - strand, within Pf1N1B4_477 at 569.264 kb on + strand, within Pf1N1B4_477 at 569.264 kb on + strand, within Pf1N1B4_477 at 569.265 kb on - strand, within Pf1N1B4_477 at 569.605 kb on + strand, within Pf1N1B4_477 at 569.606 kb on - strand at 569.606 kb on - strand, within Pf1N1B4_477 at 569.754 kb on - strand at 569.901 kb on + strand, within Pf1N1B4_478 at 569.902 kb on - strand, within Pf1N1B4_478 at 569.902 kb on - strand, within Pf1N1B4_478 at 569.902 kb on - strand, within Pf1N1B4_478 at 569.931 kb on + strand, within Pf1N1B4_478 at 569.931 kb on + strand, within Pf1N1B4_478 at 569.932 kb on - strand, within Pf1N1B4_478
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 567,111 + +0.5 567,111 + +0.6 567,151 + -0.6 567,152 - -0.5 567,152 - +0.1 567,155 + +1.9 567,155 + +1.1 567,155 + +0.6 567,155 + +0.0 567,155 + -0.1 567,155 + -0.6 567,155 + -1.9 567,155 + -1.4 567,155 + +0.2 567,155 + -1.4 567,155 + +1.0 567,155 + +1.4 567,155 + -1.9 567,155 + +2.2 567,155 + -1.6 567,155 + -0.6 567,155 + +0.7 567,156 - +0.9 567,156 - -1.6 567,156 - +0.7 567,156 - -1.2 567,156 - -1.1 567,156 - +1.4 567,156 - -0.3 567,156 - +1.9 567,156 - +1.3 567,156 - +1.5 567,156 - +0.9 567,156 - +1.6 567,156 - -1.6 567,156 - -1.2 567,156 - +0.9 567,156 - -0.4 567,156 - -1.1 567,156 - -0.1 567,156 - -0.0 567,156 - +0.7 567,213 - +0.4 567,213 - -2.1 567,224 - -2.1 567,250 + +0.9 567,251 - -0.6 567,303 + +0.4 567,303 + -0.2 567,303 + -0.1 567,303 + -0.6 567,304 - +1.5 567,304 - +0.5 567,304 - +0.5 567,304 - -0.5 567,304 - -1.5 567,304 - +0.6 567,304 - -1.9 567,304 - -2.2 567,304 - -2.2 567,304 - +0.2 567,304 - -1.9 567,321 - +2.5 567,460 - Pf1N1B4_475 0.26 -0.4 567,518 - Pf1N1B4_475 0.34 +0.9 567,700 - -0.1 567,798 + Pf1N1B4_475 0.70 +0.7 567,843 + Pf1N1B4_475 0.76 +0.4 567,843 + Pf1N1B4_475 0.76 -0.3 567,844 - Pf1N1B4_475 0.76 -0.6 567,852 + +0.9 567,852 + Pf1N1B4_475 0.77 -1.6 567,852 + Pf1N1B4_475 0.77 -1.1 567,853 - Pf1N1B4_475 0.77 -0.4 567,853 - Pf1N1B4_475 0.77 -0.4 567,912 + Pf1N1B4_475 0.84 -0.4 567,913 - Pf1N1B4_475 0.84 +0.9 567,995 - +0.2 568,012 + -0.5 568,012 + +0.3 568,012 + -1.4 568,013 - -0.6 568,156 - Pf1N1B4_476 0.14 -0.7 568,191 + Pf1N1B4_476 0.17 -0.2 568,605 - Pf1N1B4_476 0.62 -1.1 568,646 + Pf1N1B4_476 0.66 -1.2 568,649 + Pf1N1B4_476 0.66 -1.1 568,650 - Pf1N1B4_476 0.66 -0.1 568,773 + Pf1N1B4_476 0.80 -1.5 568,773 + Pf1N1B4_476 0.80 -0.1 568,774 - +1.5 568,996 + -1.1 568,997 - +2.3 568,997 - -0.2 569,000 + -0.1 569,000 + -1.1 569,001 - -0.6 569,001 - +0.7 569,001 - +1.7 569,020 + -0.1 569,020 + -1.4 569,020 + -2.5 569,020 + -0.5 569,021 - -0.4 569,068 + -0.5 569,068 + +0.3 569,068 + -0.9 569,069 - +0.7 569,069 - +1.5 569,069 - -1.9 569,069 - -0.9 569,094 - -1.1 569,094 - -0.9 569,096 + +0.9 569,096 + -1.2 569,096 + -0.8 569,097 - +1.9 569,097 - -0.1 569,097 - +0.7 569,097 - -0.6 569,097 - +0.9 569,097 - -1.1 569,097 - -1.6 569,097 - +2.9 569,097 - +0.5 569,097 - +1.1 569,151 + +0.9 569,152 - -1.6 569,153 + Pf1N1B4_477 0.10 +1.2 569,153 + Pf1N1B4_477 0.10 -1.3 569,154 - Pf1N1B4_477 0.10 +0.1 569,154 - Pf1N1B4_477 0.10 +0.4 569,210 + Pf1N1B4_477 0.20 -1.6 569,210 + Pf1N1B4_477 0.20 -0.6 569,210 + Pf1N1B4_477 0.20 -0.6 569,210 + -0.4 569,211 - Pf1N1B4_477 0.20 -0.2 569,211 - Pf1N1B4_477 0.20 -0.6 569,264 + Pf1N1B4_477 0.29 -2.2 569,264 + Pf1N1B4_477 0.29 +0.5 569,265 - Pf1N1B4_477 0.30 +0.5 569,605 + Pf1N1B4_477 0.89 -1.9 569,606 - +0.4 569,606 - Pf1N1B4_477 0.89 -2.1 569,754 - -1.4 569,901 + Pf1N1B4_478 0.21 +0.2 569,902 - Pf1N1B4_478 0.21 -1.6 569,902 - Pf1N1B4_478 0.21 -1.9 569,902 - Pf1N1B4_478 0.21 +0.8 569,931 + Pf1N1B4_478 0.26 +0.4 569,931 + Pf1N1B4_478 0.26 +0.5 569,932 - Pf1N1B4_478 0.26 +0.1
Or see this region's nucleotide sequence