Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4600 and Pf1N1B4_4601 are separated by 14 nucleotides Pf1N1B4_4601 and Pf1N1B4_4602 are separated by 76 nucleotides Pf1N1B4_4602 and Pf1N1B4_4603 are separated by 88 nucleotides Pf1N1B4_4603 and Pf1N1B4_4604 are separated by 244 nucleotides
Pf1N1B4_4600 - Lysine decarboxylase, inducible (EC 4.1.1.18), at 5,072,925 to 5,075,180
_4600
Pf1N1B4_4601 - Streptothricin acetyltransferase, Streptomyces lavendulae type, at 5,075,195 to 5,075,698
_4601
Pf1N1B4_4602 - Transcriptional regulators, LysR family, at 5,075,775 to 5,076,668
_4602
Pf1N1B4_4603 - hypothetical protein, at 5,076,757 to 5,076,933
_4603
Pf1N1B4_4604 - FMN reductase, NADPH-dependent, at 5,077,178 to 5,077,738
_4604
Position (kb) 5075
5076
5077 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5074.863 kb on + strand at 5074.863 kb on + strand, within Pf1N1B4_4600 at 5074.863 kb on + strand, within Pf1N1B4_4600 at 5074.864 kb on - strand, within Pf1N1B4_4600 at 5075.058 kb on + strand at 5075.154 kb on + strand at 5075.154 kb on + strand at 5075.154 kb on + strand at 5075.154 kb on + strand at 5075.155 kb on - strand at 5075.220 kb on - strand at 5075.264 kb on + strand, within Pf1N1B4_4601 at 5075.265 kb on - strand, within Pf1N1B4_4601 at 5075.265 kb on - strand, within Pf1N1B4_4601 at 5075.265 kb on - strand at 5075.265 kb on - strand, within Pf1N1B4_4601 at 5075.266 kb on + strand, within Pf1N1B4_4601 at 5075.266 kb on + strand, within Pf1N1B4_4601 at 5075.267 kb on - strand, within Pf1N1B4_4601 at 5075.869 kb on + strand, within Pf1N1B4_4602 at 5076.131 kb on - strand, within Pf1N1B4_4602 at 5076.131 kb on - strand, within Pf1N1B4_4602 at 5076.406 kb on + strand, within Pf1N1B4_4602 at 5076.406 kb on + strand, within Pf1N1B4_4602 at 5076.454 kb on + strand, within Pf1N1B4_4602 at 5076.733 kb on - strand at 5076.864 kb on + strand, within Pf1N1B4_4603 at 5076.866 kb on + strand, within Pf1N1B4_4603 at 5076.866 kb on + strand, within Pf1N1B4_4603 at 5076.867 kb on - strand, within Pf1N1B4_4603 at 5077.038 kb on + strand at 5077.058 kb on + strand at 5077.136 kb on + strand at 5077.136 kb on + strand at 5077.137 kb on - strand at 5077.137 kb on - strand at 5077.137 kb on - strand at 5077.137 kb on - strand at 5077.138 kb on + strand at 5077.138 kb on + strand at 5077.138 kb on + strand at 5077.139 kb on - strand at 5077.142 kb on + strand at 5077.142 kb on + strand at 5077.142 kb on + strand at 5077.142 kb on + strand at 5077.142 kb on + strand at 5077.143 kb on - strand at 5077.190 kb on + strand at 5077.320 kb on - strand, within Pf1N1B4_4604 at 5077.418 kb on + strand, within Pf1N1B4_4604 at 5077.418 kb on + strand, within Pf1N1B4_4604 at 5077.616 kb on + strand, within Pf1N1B4_4604 at 5077.617 kb on - strand, within Pf1N1B4_4604
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 5,074,863 + +1.6 5,074,863 + Pf1N1B4_4600 0.86 +0.4 5,074,863 + Pf1N1B4_4600 0.86 +1.1 5,074,864 - Pf1N1B4_4600 0.86 +1.4 5,075,058 + -0.4 5,075,154 + -1.2 5,075,154 + +0.4 5,075,154 + +0.4 5,075,154 + -1.8 5,075,155 - -0.5 5,075,220 - -2.2 5,075,264 + Pf1N1B4_4601 0.14 -0.3 5,075,265 - Pf1N1B4_4601 0.14 -0.2 5,075,265 - Pf1N1B4_4601 0.14 -2.8 5,075,265 - +0.8 5,075,265 - Pf1N1B4_4601 0.14 +0.1 5,075,266 + Pf1N1B4_4601 0.14 +0.8 5,075,266 + Pf1N1B4_4601 0.14 -0.2 5,075,267 - Pf1N1B4_4601 0.14 +0.4 5,075,869 + Pf1N1B4_4602 0.11 -0.2 5,076,131 - Pf1N1B4_4602 0.40 +0.4 5,076,131 - Pf1N1B4_4602 0.40 -2.2 5,076,406 + Pf1N1B4_4602 0.71 +0.6 5,076,406 + Pf1N1B4_4602 0.71 -1.6 5,076,454 + Pf1N1B4_4602 0.76 -2.6 5,076,733 - -0.8 5,076,864 + Pf1N1B4_4603 0.60 -1.6 5,076,866 + Pf1N1B4_4603 0.62 -1.1 5,076,866 + Pf1N1B4_4603 0.62 -1.2 5,076,867 - Pf1N1B4_4603 0.62 -0.6 5,077,038 + -0.1 5,077,058 + +1.4 5,077,136 + +0.4 5,077,136 + +1.0 5,077,137 - -1.6 5,077,137 - +0.0 5,077,137 - +1.0 5,077,137 - -1.6 5,077,138 + +1.4 5,077,138 + +0.8 5,077,138 + +1.0 5,077,139 - +0.4 5,077,142 + +1.1 5,077,142 + +0.4 5,077,142 + +1.1 5,077,142 + -0.6 5,077,142 + +0.4 5,077,143 - -0.8 5,077,190 + -0.0 5,077,320 - Pf1N1B4_4604 0.25 +0.5 5,077,418 + Pf1N1B4_4604 0.43 +0.3 5,077,418 + Pf1N1B4_4604 0.43 -0.6 5,077,616 + Pf1N1B4_4604 0.78 -0.6 5,077,617 - Pf1N1B4_4604 0.78 -0.3
Or see this region's nucleotide sequence