Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4223 and Pf1N1B4_4224 are separated by 306 nucleotides Pf1N1B4_4224 and Pf1N1B4_4225 are separated by 149 nucleotides Pf1N1B4_4225 and Pf1N1B4_4226 are separated by 79 nucleotides
Pf1N1B4_4223 - Transcriptional regulator, at 4,679,386 to 4,680,279
_4223
Pf1N1B4_4224 - putative tautomerase, at 4,680,586 to 4,680,795
_4224
Pf1N1B4_4225 - transcriptional regulator, AraC family, at 4,680,945 to 4,681,907
_4225
Pf1N1B4_4226 - Sterol desaturase family protein, at 4,681,987 to 4,682,829
_4226
Position (kb)
4680
4681
4682 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4680.137 kb on - strand, within Pf1N1B4_4223 at 4680.244 kb on - strand at 4680.303 kb on + strand at 4680.380 kb on - strand at 4680.403 kb on - strand at 4680.433 kb on + strand at 4680.433 kb on + strand at 4680.437 kb on + strand at 4680.437 kb on + strand at 4680.438 kb on - strand at 4680.542 kb on + strand at 4680.793 kb on + strand at 4680.946 kb on + strand at 4680.946 kb on + strand at 4680.946 kb on + strand at 4680.947 kb on - strand at 4680.982 kb on + strand at 4680.982 kb on + strand at 4681.083 kb on + strand at 4681.083 kb on + strand, within Pf1N1B4_4225 at 4681.396 kb on + strand, within Pf1N1B4_4225 at 4681.405 kb on + strand, within Pf1N1B4_4225 at 4681.499 kb on - strand, within Pf1N1B4_4225 at 4681.657 kb on + strand, within Pf1N1B4_4225 at 4681.658 kb on - strand, within Pf1N1B4_4225 at 4681.669 kb on + strand at 4681.670 kb on - strand, within Pf1N1B4_4225 at 4681.670 kb on - strand at 4681.772 kb on + strand, within Pf1N1B4_4225 at 4681.882 kb on - strand at 4681.882 kb on - strand at 4681.980 kb on + strand at 4681.980 kb on + strand at 4681.982 kb on + strand at 4681.983 kb on - strand at 4682.148 kb on - strand, within Pf1N1B4_4226 at 4682.172 kb on - strand, within Pf1N1B4_4226 at 4682.246 kb on + strand, within Pf1N1B4_4226 at 4682.270 kb on - strand, within Pf1N1B4_4226 at 4682.341 kb on + strand, within Pf1N1B4_4226 at 4682.342 kb on - strand, within Pf1N1B4_4226 at 4682.342 kb on - strand at 4682.343 kb on + strand at 4682.344 kb on - strand, within Pf1N1B4_4226 at 4682.344 kb on - strand, within Pf1N1B4_4226 at 4682.344 kb on - strand, within Pf1N1B4_4226 at 4682.376 kb on + strand, within Pf1N1B4_4226 at 4682.377 kb on - strand, within Pf1N1B4_4226 at 4682.381 kb on - strand, within Pf1N1B4_4226 at 4682.383 kb on + strand, within Pf1N1B4_4226 at 4682.384 kb on - strand, within Pf1N1B4_4226 at 4682.425 kb on + strand, within Pf1N1B4_4226 at 4682.426 kb on - strand, within Pf1N1B4_4226 at 4682.426 kb on - strand, within Pf1N1B4_4226 at 4682.426 kb on - strand, within Pf1N1B4_4226 at 4682.447 kb on - strand, within Pf1N1B4_4226 at 4682.449 kb on + strand, within Pf1N1B4_4226 at 4682.450 kb on - strand, within Pf1N1B4_4226 at 4682.632 kb on + strand, within Pf1N1B4_4226 at 4682.633 kb on - strand, within Pf1N1B4_4226 at 4682.759 kb on + strand at 4682.759 kb on + strand at 4682.760 kb on - strand at 4682.760 kb on - strand at 4682.801 kb on + strand at 4682.837 kb on + strand at 4682.838 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 4,680,137 - Pf1N1B4_4223 0.84 +0.9 4,680,244 - +1.6 4,680,303 + +1.6 4,680,380 - +0.6 4,680,403 - -0.5 4,680,433 + +0.0 4,680,433 + -0.7 4,680,437 + -1.2 4,680,437 + +0.6 4,680,438 - +0.6 4,680,542 + +0.5 4,680,793 + +0.6 4,680,946 + +0.7 4,680,946 + +0.3 4,680,946 + +0.6 4,680,947 - -1.4 4,680,982 + +1.6 4,680,982 + -1.2 4,681,083 + -1.4 4,681,083 + Pf1N1B4_4225 0.14 +0.6 4,681,396 + Pf1N1B4_4225 0.47 +0.0 4,681,405 + Pf1N1B4_4225 0.48 -0.4 4,681,499 - Pf1N1B4_4225 0.58 -0.5 4,681,657 + Pf1N1B4_4225 0.74 -2.6 4,681,658 - Pf1N1B4_4225 0.74 -1.6 4,681,669 + +2.3 4,681,670 - Pf1N1B4_4225 0.75 -1.8 4,681,670 - -0.4 4,681,772 + Pf1N1B4_4225 0.86 +0.0 4,681,882 - -0.2 4,681,882 - -0.1 4,681,980 + -0.7 4,681,980 + -0.1 4,681,982 + -1.7 4,681,983 - +0.2 4,682,148 - Pf1N1B4_4226 0.19 -1.0 4,682,172 - Pf1N1B4_4226 0.22 +2.0 4,682,246 + Pf1N1B4_4226 0.31 -0.1 4,682,270 - Pf1N1B4_4226 0.34 -0.2 4,682,341 + Pf1N1B4_4226 0.42 -1.0 4,682,342 - Pf1N1B4_4226 0.42 +0.4 4,682,342 - +0.6 4,682,343 + -0.4 4,682,344 - Pf1N1B4_4226 0.42 +2.4 4,682,344 - Pf1N1B4_4226 0.42 -1.4 4,682,344 - Pf1N1B4_4226 0.42 -1.2 4,682,376 + Pf1N1B4_4226 0.46 +0.7 4,682,377 - Pf1N1B4_4226 0.46 -2.4 4,682,381 - Pf1N1B4_4226 0.47 +1.8 4,682,383 + Pf1N1B4_4226 0.47 +0.2 4,682,384 - Pf1N1B4_4226 0.47 +1.6 4,682,425 + Pf1N1B4_4226 0.52 -0.7 4,682,426 - Pf1N1B4_4226 0.52 -0.1 4,682,426 - Pf1N1B4_4226 0.52 -0.1 4,682,426 - Pf1N1B4_4226 0.52 +1.2 4,682,447 - Pf1N1B4_4226 0.55 -0.2 4,682,449 + Pf1N1B4_4226 0.55 -0.3 4,682,450 - Pf1N1B4_4226 0.55 +0.4 4,682,632 + Pf1N1B4_4226 0.77 +0.4 4,682,633 - Pf1N1B4_4226 0.77 -0.2 4,682,759 + -1.4 4,682,759 + -0.7 4,682,760 - -1.0 4,682,760 - +0.2 4,682,801 + +1.3 4,682,837 + -2.4 4,682,838 - +0.7
Or see this region's nucleotide sequence