Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_398 and Pf1N1B4_399 are separated by 46 nucleotides Pf1N1B4_399 and Pf1N1B4_400 are separated by 19 nucleotides
Pf1N1B4_398 - Galactonate dehydratase (EC 4.2.1.6), at 467,549 to 468,697
_398
Pf1N1B4_399 - 2-dehydro-3-deoxyphosphogalactonate aldolase (EC 4.1.2.21), at 468,744 to 469,364
_399
Pf1N1B4_400 - 2-dehydro-3-deoxygalactonokinase (EC 2.7.1.58), at 469,384 to 470,376
_400
Position (kb) 468
469
470 Strain fitness (log2 ratio) -2
-1
0
1
2 at 467.748 kb on + strand, within Pf1N1B4_398 at 467.748 kb on + strand, within Pf1N1B4_398 at 467.748 kb on + strand, within Pf1N1B4_398 at 467.748 kb on + strand, within Pf1N1B4_398 at 467.749 kb on - strand, within Pf1N1B4_398 at 467.749 kb on - strand, within Pf1N1B4_398 at 467.865 kb on + strand, within Pf1N1B4_398 at 467.866 kb on - strand, within Pf1N1B4_398 at 467.866 kb on - strand, within Pf1N1B4_398 at 467.946 kb on + strand, within Pf1N1B4_398 at 467.946 kb on + strand, within Pf1N1B4_398 at 467.946 kb on + strand, within Pf1N1B4_398 at 467.947 kb on - strand, within Pf1N1B4_398 at 468.091 kb on - strand, within Pf1N1B4_398 at 468.130 kb on + strand, within Pf1N1B4_398 at 468.348 kb on + strand, within Pf1N1B4_398 at 468.349 kb on - strand, within Pf1N1B4_398 at 468.349 kb on - strand, within Pf1N1B4_398 at 468.480 kb on + strand, within Pf1N1B4_398 at 468.480 kb on + strand, within Pf1N1B4_398 at 468.480 kb on + strand, within Pf1N1B4_398 at 468.495 kb on + strand, within Pf1N1B4_398 at 468.546 kb on + strand, within Pf1N1B4_398 at 468.547 kb on - strand at 468.547 kb on - strand, within Pf1N1B4_398 at 468.699 kb on + strand at 468.700 kb on - strand at 468.700 kb on - strand at 468.700 kb on - strand at 468.700 kb on - strand at 468.712 kb on - strand at 468.712 kb on - strand at 468.712 kb on - strand at 468.712 kb on - strand at 468.712 kb on - strand at 468.714 kb on + strand at 468.714 kb on + strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.715 kb on - strand at 468.772 kb on + strand at 468.772 kb on + strand at 468.772 kb on + strand at 468.772 kb on + strand at 468.773 kb on - strand at 468.773 kb on - strand at 468.773 kb on - strand at 468.773 kb on - strand at 469.196 kb on + strand, within Pf1N1B4_399 at 469.197 kb on - strand, within Pf1N1B4_399 at 469.214 kb on + strand, within Pf1N1B4_399 at 469.222 kb on + strand, within Pf1N1B4_399 at 469.223 kb on - strand, within Pf1N1B4_399 at 469.239 kb on - strand, within Pf1N1B4_399 at 469.274 kb on - strand, within Pf1N1B4_399 at 469.629 kb on - strand, within Pf1N1B4_400 at 470.324 kb on + strand at 470.324 kb on + strand at 470.324 kb on + strand at 470.324 kb on + strand at 470.325 kb on - strand at 470.332 kb on + strand at 470.332 kb on + strand at 470.333 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 467,748 + Pf1N1B4_398 0.17 +0.6 467,748 + Pf1N1B4_398 0.17 -0.3 467,748 + Pf1N1B4_398 0.17 -1.1 467,748 + Pf1N1B4_398 0.17 +1.4 467,749 - Pf1N1B4_398 0.17 -1.9 467,749 - Pf1N1B4_398 0.17 -1.9 467,865 + Pf1N1B4_398 0.28 +1.1 467,866 - Pf1N1B4_398 0.28 +0.2 467,866 - Pf1N1B4_398 0.28 -0.9 467,946 + Pf1N1B4_398 0.35 +0.8 467,946 + Pf1N1B4_398 0.35 +0.6 467,946 + Pf1N1B4_398 0.35 -0.9 467,947 - Pf1N1B4_398 0.35 -0.9 468,091 - Pf1N1B4_398 0.47 -0.4 468,130 + Pf1N1B4_398 0.51 +0.3 468,348 + Pf1N1B4_398 0.70 +0.1 468,349 - Pf1N1B4_398 0.70 -0.1 468,349 - Pf1N1B4_398 0.70 +0.3 468,480 + Pf1N1B4_398 0.81 -0.8 468,480 + Pf1N1B4_398 0.81 -0.1 468,480 + Pf1N1B4_398 0.81 +1.2 468,495 + Pf1N1B4_398 0.82 +0.3 468,546 + Pf1N1B4_398 0.87 -1.1 468,547 - +0.9 468,547 - Pf1N1B4_398 0.87 -0.1 468,699 + -1.7 468,700 - -0.7 468,700 - +1.1 468,700 - +0.5 468,700 - +0.9 468,712 - +1.9 468,712 - -0.9 468,712 - +0.3 468,712 - +1.5 468,712 - -0.1 468,714 + -0.0 468,714 + +0.3 468,715 - +0.9 468,715 - +1.5 468,715 - -1.4 468,715 - +0.2 468,715 - -0.3 468,715 - -0.7 468,715 - +1.9 468,715 - -1.6 468,772 + -0.1 468,772 + -0.7 468,772 + -0.1 468,772 + -0.4 468,773 - -0.1 468,773 - +0.3 468,773 - +1.5 468,773 - -0.1 469,196 + Pf1N1B4_399 0.73 +0.3 469,197 - Pf1N1B4_399 0.73 -1.4 469,214 + Pf1N1B4_399 0.76 -1.7 469,222 + Pf1N1B4_399 0.77 +0.2 469,223 - Pf1N1B4_399 0.77 -1.7 469,239 - Pf1N1B4_399 0.80 -0.1 469,274 - Pf1N1B4_399 0.85 -2.1 469,629 - Pf1N1B4_400 0.25 -2.1 470,324 + +0.9 470,324 + -0.1 470,324 + +0.9 470,324 + -0.0 470,325 - -1.4 470,332 + -1.4 470,332 + -0.5 470,333 - -0.7
Or see this region's nucleotide sequence