Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_362 and Pf1N1B4_363 overlap by 8 nucleotides Pf1N1B4_363 and Pf1N1B4_364 are separated by 3 nucleotides Pf1N1B4_364 and Pf1N1B4_365 are separated by 286 nucleotides Pf1N1B4_365 and Pf1N1B4_366 are separated by 12 nucleotides
Pf1N1B4_362 - Lipid A export ATP-binding/permease protein MsbA, at 430,596 to 432,413
_362
Pf1N1B4_363 - Thioredoxin, at 432,406 to 433,044
_363
Pf1N1B4_364 - Rhodanese domain protein UPF0176, at 433,048 to 433,989
_364
Pf1N1B4_365 - Cell division protein BolA, at 434,276 to 434,575
_365
Pf1N1B4_366 - FIG00954136: hypothetical protein, at 434,588 to 435,109
_366
Position (kb) 433
434 Strain fitness (log2 ratio) -2
-1
0
1
2 at 432.154 kb on + strand at 432.471 kb on - strand, within Pf1N1B4_363 at 432.501 kb on + strand, within Pf1N1B4_363 at 432.502 kb on - strand, within Pf1N1B4_363 at 432.687 kb on - strand, within Pf1N1B4_363 at 432.687 kb on - strand, within Pf1N1B4_363 at 432.782 kb on + strand, within Pf1N1B4_363 at 432.865 kb on - strand, within Pf1N1B4_363 at 432.867 kb on - strand, within Pf1N1B4_363 at 432.867 kb on - strand, within Pf1N1B4_363 at 432.867 kb on - strand, within Pf1N1B4_363 at 432.937 kb on + strand, within Pf1N1B4_363 at 433.022 kb on + strand at 433.022 kb on + strand at 433.022 kb on + strand at 433.023 kb on - strand at 433.023 kb on - strand at 433.023 kb on - strand at 433.023 kb on - strand at 433.023 kb on - strand at 433.023 kb on - strand at 433.073 kb on + strand at 433.074 kb on - strand at 433.074 kb on - strand at 433.074 kb on - strand at 433.074 kb on - strand at 433.074 kb on - strand at 433.079 kb on + strand at 433.079 kb on + strand at 433.080 kb on - strand at 433.205 kb on + strand, within Pf1N1B4_364 at 433.205 kb on + strand, within Pf1N1B4_364 at 433.206 kb on - strand, within Pf1N1B4_364 at 433.206 kb on - strand, within Pf1N1B4_364 at 433.206 kb on - strand, within Pf1N1B4_364 at 433.392 kb on - strand, within Pf1N1B4_364 at 433.421 kb on + strand, within Pf1N1B4_364 at 433.509 kb on - strand, within Pf1N1B4_364 at 433.652 kb on + strand, within Pf1N1B4_364 at 433.653 kb on - strand, within Pf1N1B4_364 at 433.653 kb on - strand, within Pf1N1B4_364 at 433.934 kb on + strand at 433.956 kb on + strand at 433.956 kb on + strand at 433.958 kb on + strand at 433.958 kb on + strand at 433.959 kb on - strand at 433.994 kb on - strand at 433.994 kb on - strand at 434.017 kb on + strand at 434.018 kb on - strand at 434.076 kb on - strand at 434.204 kb on - strand at 434.204 kb on - strand at 434.204 kb on - strand at 434.277 kb on + strand at 434.277 kb on + strand at 434.277 kb on + strand at 434.278 kb on - strand at 434.349 kb on + strand, within Pf1N1B4_365 at 434.349 kb on + strand, within Pf1N1B4_365 at 434.349 kb on + strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.403 kb on + strand, within Pf1N1B4_365 at 434.404 kb on - strand, within Pf1N1B4_365 at 434.404 kb on - strand, within Pf1N1B4_365 at 434.590 kb on - strand at 434.620 kb on + strand at 434.761 kb on + strand, within Pf1N1B4_366 at 434.763 kb on + strand, within Pf1N1B4_366 at 434.763 kb on + strand, within Pf1N1B4_366 at 434.763 kb on + strand, within Pf1N1B4_366 at 434.763 kb on + strand, within Pf1N1B4_366 at 434.764 kb on - strand, within Pf1N1B4_366 at 434.764 kb on - strand, within Pf1N1B4_366 at 434.811 kb on + strand, within Pf1N1B4_366 at 434.811 kb on + strand, within Pf1N1B4_366 at 434.811 kb on + strand, within Pf1N1B4_366 at 434.811 kb on + strand, within Pf1N1B4_366 at 434.812 kb on - strand, within Pf1N1B4_366 at 434.812 kb on - strand, within Pf1N1B4_366 at 434.812 kb on - strand, within Pf1N1B4_366 at 434.812 kb on - strand, within Pf1N1B4_366 at 434.812 kb on - strand, within Pf1N1B4_366 at 434.812 kb on - strand, within Pf1N1B4_366 at 434.886 kb on + strand, within Pf1N1B4_366 at 434.886 kb on + strand at 434.886 kb on + strand, within Pf1N1B4_366 at 434.886 kb on + strand, within Pf1N1B4_366 at 434.886 kb on + strand, within Pf1N1B4_366 at 434.886 kb on + strand at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.968 kb on - strand, within Pf1N1B4_366 at 434.968 kb on - strand, within Pf1N1B4_366 at 434.968 kb on - strand, within Pf1N1B4_366 at 434.968 kb on - strand, within Pf1N1B4_366
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 432,154 + +0.9 432,471 - Pf1N1B4_363 0.10 -0.5 432,501 + Pf1N1B4_363 0.15 +0.2 432,502 - Pf1N1B4_363 0.15 +0.9 432,687 - Pf1N1B4_363 0.44 -1.7 432,687 - Pf1N1B4_363 0.44 -1.5 432,782 + Pf1N1B4_363 0.59 -1.1 432,865 - Pf1N1B4_363 0.72 -0.6 432,867 - Pf1N1B4_363 0.72 +0.9 432,867 - Pf1N1B4_363 0.72 +1.4 432,867 - Pf1N1B4_363 0.72 -0.1 432,937 + Pf1N1B4_363 0.83 -0.1 433,022 + +0.9 433,022 + -0.1 433,022 + -0.9 433,023 - +0.0 433,023 - -0.7 433,023 - +0.3 433,023 - +0.9 433,023 - +0.3 433,023 - +0.0 433,073 + -0.1 433,074 - -1.1 433,074 - +1.3 433,074 - +1.3 433,074 - +0.9 433,074 - +0.9 433,079 + +0.3 433,079 + +0.9 433,080 - -1.5 433,205 + Pf1N1B4_364 0.17 +0.9 433,205 + Pf1N1B4_364 0.17 -0.1 433,206 - Pf1N1B4_364 0.17 -1.2 433,206 - Pf1N1B4_364 0.17 -0.9 433,206 - Pf1N1B4_364 0.17 -1.8 433,392 - Pf1N1B4_364 0.37 -2.2 433,421 + Pf1N1B4_364 0.40 +0.9 433,509 - Pf1N1B4_364 0.49 -1.1 433,652 + Pf1N1B4_364 0.64 +0.9 433,653 - Pf1N1B4_364 0.64 -0.7 433,653 - Pf1N1B4_364 0.64 -0.1 433,934 + +1.4 433,956 + -0.7 433,956 + -1.1 433,958 + +0.9 433,958 + -2.3 433,959 - +0.1 433,994 - -0.1 433,994 - +0.9 434,017 + -1.7 434,018 - +1.9 434,076 - -2.1 434,204 - +0.9 434,204 - +0.1 434,204 - -1.1 434,277 + +1.9 434,277 + +1.4 434,277 + +0.4 434,278 - +0.0 434,349 + Pf1N1B4_365 0.24 -1.5 434,349 + Pf1N1B4_365 0.24 +0.3 434,349 + Pf1N1B4_365 0.24 +0.4 434,350 - Pf1N1B4_365 0.25 -2.1 434,350 - Pf1N1B4_365 0.25 -0.1 434,350 - Pf1N1B4_365 0.25 +0.1 434,350 - Pf1N1B4_365 0.25 +0.1 434,350 - Pf1N1B4_365 0.25 -0.1 434,350 - Pf1N1B4_365 0.25 +2.4 434,350 - Pf1N1B4_365 0.25 +0.9 434,350 - Pf1N1B4_365 0.25 +0.7 434,350 - Pf1N1B4_365 0.25 -2.1 434,350 - Pf1N1B4_365 0.25 -1.0 434,350 - Pf1N1B4_365 0.25 -0.9 434,350 - Pf1N1B4_365 0.25 +0.1 434,350 - Pf1N1B4_365 0.25 +1.2 434,403 + Pf1N1B4_365 0.42 -0.7 434,404 - Pf1N1B4_365 0.43 -1.9 434,404 - Pf1N1B4_365 0.43 +1.2 434,590 - +0.4 434,620 + -1.8 434,761 + Pf1N1B4_366 0.33 -0.1 434,763 + Pf1N1B4_366 0.34 -0.5 434,763 + Pf1N1B4_366 0.34 +0.4 434,763 + Pf1N1B4_366 0.34 -0.6 434,763 + Pf1N1B4_366 0.34 -0.6 434,764 - Pf1N1B4_366 0.34 +0.1 434,764 - Pf1N1B4_366 0.34 -0.3 434,811 + Pf1N1B4_366 0.43 -1.4 434,811 + Pf1N1B4_366 0.43 -0.0 434,811 + Pf1N1B4_366 0.43 -0.4 434,811 + Pf1N1B4_366 0.43 +0.4 434,812 - Pf1N1B4_366 0.43 -1.4 434,812 - Pf1N1B4_366 0.43 +0.9 434,812 - Pf1N1B4_366 0.43 +0.0 434,812 - Pf1N1B4_366 0.43 +0.0 434,812 - Pf1N1B4_366 0.43 -1.1 434,812 - Pf1N1B4_366 0.43 -1.5 434,886 + Pf1N1B4_366 0.57 -1.5 434,886 + +1.4 434,886 + Pf1N1B4_366 0.57 -0.3 434,886 + Pf1N1B4_366 0.57 -0.1 434,886 + Pf1N1B4_366 0.57 +0.3 434,886 + -0.1 434,887 - Pf1N1B4_366 0.57 -0.7 434,887 - Pf1N1B4_366 0.57 -1.5 434,887 - Pf1N1B4_366 0.57 -0.5 434,887 - Pf1N1B4_366 0.57 -1.7 434,887 - Pf1N1B4_366 0.57 -0.7 434,887 - Pf1N1B4_366 0.57 +0.4 434,887 - Pf1N1B4_366 0.57 -0.8 434,887 - Pf1N1B4_366 0.57 +0.9 434,967 + Pf1N1B4_366 0.73 -1.0 434,967 + Pf1N1B4_366 0.73 -1.4 434,967 + Pf1N1B4_366 0.73 -1.3 434,967 + Pf1N1B4_366 0.73 -1.9 434,967 + Pf1N1B4_366 0.73 +0.9 434,967 + Pf1N1B4_366 0.73 +1.9 434,967 + Pf1N1B4_366 0.73 -0.1 434,967 + Pf1N1B4_366 0.73 +0.1 434,967 + Pf1N1B4_366 0.73 -0.3 434,967 + Pf1N1B4_366 0.73 -1.5 434,967 + Pf1N1B4_366 0.73 -1.7 434,967 + Pf1N1B4_366 0.73 -0.2 434,968 - Pf1N1B4_366 0.73 +0.1 434,968 - Pf1N1B4_366 0.73 -2.5 434,968 - Pf1N1B4_366 0.73 -1.5 434,968 - Pf1N1B4_366 0.73 -1.9
Or see this region's nucleotide sequence