Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3622 and Pf1N1B4_3623 are separated by 11 nucleotides Pf1N1B4_3623 and Pf1N1B4_3624 overlap by 34 nucleotides Pf1N1B4_3624 and Pf1N1B4_3625 are separated by 233 nucleotides Pf1N1B4_3625 and Pf1N1B4_3626 overlap by 4 nucleotides
Pf1N1B4_3622 - Flagellar biosynthesis protein FlhA, at 4,029,868 to 4,031,997
_3622
Pf1N1B4_3623 - Flagellar biosynthesis protein FlhF, at 4,032,009 to 4,032,446
_3623
Pf1N1B4_3624 - Flagellar biosynthesis protein FlhF, at 4,032,413 to 4,033,336
_3624
Pf1N1B4_3625 - Flagellar synthesis regulator FleN, at 4,033,570 to 4,034,283
_3625
Pf1N1B4_3626 - RNA polymerase sigma factor for flagellar operon, at 4,034,280 to 4,035,020
_3626
Position (kb)
4032
4033
4034 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4031.604 kb on + strand, within Pf1N1B4_3622 at 4031.666 kb on + strand, within Pf1N1B4_3622 at 4031.666 kb on + strand, within Pf1N1B4_3622 at 4031.667 kb on - strand at 4031.667 kb on - strand, within Pf1N1B4_3622 at 4031.720 kb on + strand, within Pf1N1B4_3622 at 4031.721 kb on - strand at 4031.730 kb on + strand at 4032.009 kb on - strand at 4032.017 kb on + strand at 4032.144 kb on + strand, within Pf1N1B4_3623 at 4032.145 kb on - strand, within Pf1N1B4_3623 at 4032.340 kb on - strand, within Pf1N1B4_3623 at 4032.340 kb on - strand, within Pf1N1B4_3623 at 4032.744 kb on - strand, within Pf1N1B4_3624 at 4032.755 kb on + strand, within Pf1N1B4_3624 at 4033.047 kb on + strand, within Pf1N1B4_3624 at 4033.048 kb on - strand, within Pf1N1B4_3624 at 4033.048 kb on - strand, within Pf1N1B4_3624 at 4033.067 kb on + strand, within Pf1N1B4_3624 at 4033.067 kb on + strand, within Pf1N1B4_3624 at 4033.067 kb on + strand, within Pf1N1B4_3624 at 4033.067 kb on + strand, within Pf1N1B4_3624 at 4033.068 kb on - strand, within Pf1N1B4_3624 at 4033.068 kb on - strand, within Pf1N1B4_3624 at 4033.102 kb on + strand, within Pf1N1B4_3624 at 4033.103 kb on - strand, within Pf1N1B4_3624 at 4033.172 kb on + strand, within Pf1N1B4_3624 at 4033.173 kb on - strand, within Pf1N1B4_3624 at 4033.251 kb on + strand at 4033.251 kb on + strand at 4033.251 kb on + strand at 4033.307 kb on + strand at 4033.307 kb on + strand at 4033.340 kb on - strand at 4033.340 kb on - strand at 4033.342 kb on + strand at 4033.342 kb on + strand at 4033.342 kb on + strand at 4033.343 kb on - strand at 4033.358 kb on + strand at 4033.358 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.362 kb on + strand at 4033.363 kb on - strand at 4033.363 kb on - strand at 4033.363 kb on - strand at 4033.428 kb on - strand at 4033.431 kb on + strand at 4033.431 kb on + strand at 4033.431 kb on + strand at 4033.431 kb on + strand at 4033.432 kb on - strand at 4033.432 kb on - strand at 4033.432 kb on - strand at 4033.449 kb on + strand at 4033.449 kb on + strand at 4033.679 kb on + strand, within Pf1N1B4_3625 at 4033.679 kb on + strand, within Pf1N1B4_3625 at 4033.679 kb on + strand at 4033.680 kb on - strand at 4033.811 kb on + strand, within Pf1N1B4_3625 at 4033.811 kb on + strand, within Pf1N1B4_3625 at 4033.811 kb on + strand, within Pf1N1B4_3625 at 4034.066 kb on + strand, within Pf1N1B4_3625 at 4034.067 kb on - strand at 4034.067 kb on - strand, within Pf1N1B4_3625 at 4034.071 kb on + strand, within Pf1N1B4_3625 at 4034.134 kb on + strand, within Pf1N1B4_3625 at 4034.196 kb on + strand, within Pf1N1B4_3625 at 4034.279 kb on + strand at 4034.279 kb on + strand at 4034.279 kb on + strand at 4034.279 kb on + strand at 4034.279 kb on + strand at 4034.280 kb on - strand at 4034.296 kb on - strand at 4034.296 kb on - strand at 4034.296 kb on - strand at 4034.304 kb on + strand at 4034.305 kb on - strand at 4034.305 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 4,031,604 + Pf1N1B4_3622 0.82 +1.3 4,031,666 + Pf1N1B4_3622 0.84 +0.9 4,031,666 + Pf1N1B4_3622 0.84 -0.6 4,031,667 - +1.4 4,031,667 - Pf1N1B4_3622 0.84 +2.9 4,031,720 + Pf1N1B4_3622 0.87 -1.1 4,031,721 - +1.6 4,031,730 + -0.1 4,032,009 - -0.7 4,032,017 + +0.6 4,032,144 + Pf1N1B4_3623 0.31 +0.5 4,032,145 - Pf1N1B4_3623 0.31 -2.5 4,032,340 - Pf1N1B4_3623 0.76 +0.4 4,032,340 - Pf1N1B4_3623 0.76 -1.9 4,032,744 - Pf1N1B4_3624 0.36 -2.1 4,032,755 + Pf1N1B4_3624 0.37 +1.5 4,033,047 + Pf1N1B4_3624 0.69 +0.2 4,033,048 - Pf1N1B4_3624 0.69 -0.5 4,033,048 - Pf1N1B4_3624 0.69 +0.3 4,033,067 + Pf1N1B4_3624 0.71 +0.4 4,033,067 + Pf1N1B4_3624 0.71 +0.3 4,033,067 + Pf1N1B4_3624 0.71 +0.3 4,033,067 + Pf1N1B4_3624 0.71 -0.6 4,033,068 - Pf1N1B4_3624 0.71 -0.7 4,033,068 - Pf1N1B4_3624 0.71 +1.4 4,033,102 + Pf1N1B4_3624 0.75 -0.1 4,033,103 - Pf1N1B4_3624 0.75 -0.9 4,033,172 + Pf1N1B4_3624 0.82 -1.1 4,033,173 - Pf1N1B4_3624 0.82 +0.9 4,033,251 + -0.7 4,033,251 + +0.9 4,033,251 + +1.6 4,033,307 + -1.7 4,033,307 + -2.0 4,033,340 - +0.7 4,033,340 - -1.1 4,033,342 + +0.5 4,033,342 + -1.5 4,033,342 + -0.1 4,033,343 - +1.7 4,033,358 + +0.3 4,033,358 + -0.5 4,033,362 + -0.4 4,033,362 + +0.9 4,033,362 + -2.1 4,033,362 + -0.4 4,033,362 + -0.5 4,033,362 + +2.1 4,033,362 + +0.5 4,033,362 + +1.6 4,033,363 - -1.7 4,033,363 - -0.1 4,033,363 - -0.7 4,033,428 - +0.4 4,033,431 + +2.2 4,033,431 + +0.2 4,033,431 + -0.6 4,033,431 + -1.1 4,033,432 - +1.4 4,033,432 - +1.9 4,033,432 - -0.1 4,033,449 + -0.1 4,033,449 + +1.4 4,033,679 + Pf1N1B4_3625 0.15 -0.4 4,033,679 + Pf1N1B4_3625 0.15 +0.0 4,033,679 + +0.9 4,033,680 - -0.7 4,033,811 + Pf1N1B4_3625 0.34 -0.0 4,033,811 + Pf1N1B4_3625 0.34 -2.3 4,033,811 + Pf1N1B4_3625 0.34 +1.6 4,034,066 + Pf1N1B4_3625 0.69 +0.8 4,034,067 - +1.4 4,034,067 - Pf1N1B4_3625 0.70 -0.4 4,034,071 + Pf1N1B4_3625 0.70 +0.3 4,034,134 + Pf1N1B4_3625 0.79 -0.7 4,034,196 + Pf1N1B4_3625 0.88 +0.1 4,034,279 + +1.6 4,034,279 + +1.3 4,034,279 + -0.5 4,034,279 + +0.9 4,034,279 + +1.1 4,034,280 - -1.1 4,034,296 - +1.3 4,034,296 - -0.4 4,034,296 - +3.7 4,034,304 + +2.4 4,034,305 - +0.2 4,034,305 - +1.2
Or see this region's nucleotide sequence