Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3487 and Pf1N1B4_3488 are separated by 145 nucleotides Pf1N1B4_3488 and Pf1N1B4_3489 are separated by 136 nucleotides Pf1N1B4_3489 and Pf1N1B4_3490 are separated by 158 nucleotides
Pf1N1B4_3487 - Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, aspartate, at 3,894,365 to 3,895,699
_3487
Pf1N1B4_3488 - Alpha-methylacyl-CoA racemase (EC 5.1.99.4), at 3,895,845 to 3,897,026
_3488
Pf1N1B4_3489 - Protein sprT, at 3,897,163 to 3,897,657
_3489
Pf1N1B4_3490 - probable membrane protein YPO2362, at 3,897,816 to 3,898,418
_3490
Position (kb)
3895
3896
3897
3898 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3895.001 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.050 kb on - strand at 3895.050 kb on - strand, within Pf1N1B4_3487 at 3895.050 kb on - strand, within Pf1N1B4_3487 at 3895.050 kb on - strand, within Pf1N1B4_3487 at 3895.133 kb on + strand at 3895.283 kb on - strand, within Pf1N1B4_3487 at 3895.283 kb on - strand, within Pf1N1B4_3487 at 3895.292 kb on + strand, within Pf1N1B4_3487 at 3895.292 kb on + strand, within Pf1N1B4_3487 at 3895.293 kb on - strand, within Pf1N1B4_3487 at 3895.293 kb on - strand, within Pf1N1B4_3487 at 3895.333 kb on + strand, within Pf1N1B4_3487 at 3895.334 kb on - strand, within Pf1N1B4_3487 at 3895.334 kb on - strand, within Pf1N1B4_3487 at 3895.650 kb on + strand at 3895.651 kb on - strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.701 kb on + strand at 3895.702 kb on - strand at 3895.702 kb on - strand at 3895.814 kb on + strand at 3895.844 kb on + strand at 3895.844 kb on + strand at 3895.844 kb on + strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3896.025 kb on + strand at 3896.025 kb on + strand, within Pf1N1B4_3488 at 3896.025 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.238 kb on + strand, within Pf1N1B4_3488 at 3896.238 kb on + strand, within Pf1N1B4_3488 at 3896.239 kb on - strand, within Pf1N1B4_3488 at 3896.239 kb on - strand, within Pf1N1B4_3488 at 3896.268 kb on + strand, within Pf1N1B4_3488 at 3896.269 kb on - strand, within Pf1N1B4_3488 at 3896.269 kb on - strand, within Pf1N1B4_3488 at 3896.295 kb on + strand, within Pf1N1B4_3488 at 3896.296 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.553 kb on + strand, within Pf1N1B4_3488 at 3896.553 kb on + strand, within Pf1N1B4_3488 at 3896.553 kb on + strand at 3896.554 kb on - strand, within Pf1N1B4_3488 at 3896.556 kb on + strand, within Pf1N1B4_3488 at 3896.556 kb on + strand, within Pf1N1B4_3488 at 3896.724 kb on + strand, within Pf1N1B4_3488 at 3896.785 kb on + strand at 3896.785 kb on + strand, within Pf1N1B4_3488 at 3896.785 kb on + strand, within Pf1N1B4_3488 at 3896.785 kb on + strand, within Pf1N1B4_3488 at 3896.785 kb on + strand, within Pf1N1B4_3488 at 3896.786 kb on - strand, within Pf1N1B4_3488 at 3896.786 kb on - strand, within Pf1N1B4_3488 at 3896.786 kb on - strand at 3897.001 kb on + strand at 3897.020 kb on + strand at 3897.020 kb on + strand at 3897.020 kb on + strand at 3897.082 kb on + strand at 3897.165 kb on - strand at 3897.165 kb on - strand at 3897.165 kb on - strand at 3897.296 kb on + strand, within Pf1N1B4_3489 at 3897.296 kb on + strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.303 kb on - strand, within Pf1N1B4_3489 at 3897.303 kb on - strand, within Pf1N1B4_3489 at 3897.332 kb on + strand, within Pf1N1B4_3489 at 3897.332 kb on + strand, within Pf1N1B4_3489 at 3897.332 kb on + strand, within Pf1N1B4_3489 at 3897.333 kb on - strand, within Pf1N1B4_3489 at 3897.333 kb on - strand at 3897.333 kb on - strand, within Pf1N1B4_3489 at 3897.362 kb on + strand, within Pf1N1B4_3489 at 3897.758 kb on + strand at 3897.758 kb on + strand at 3897.758 kb on + strand at 3897.758 kb on + strand at 3897.817 kb on + strand at 3897.817 kb on + strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.825 kb on - strand at 3897.826 kb on + strand at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.979 kb on + strand, within Pf1N1B4_3490 at 3897.979 kb on + strand, within Pf1N1B4_3490 at 3897.979 kb on + strand at 3897.980 kb on - strand, within Pf1N1B4_3490 at 3897.989 kb on - strand, within Pf1N1B4_3490 at 3897.989 kb on - strand, within Pf1N1B4_3490
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 3,895,001 + Pf1N1B4_3487 0.48 +0.5 3,895,049 + Pf1N1B4_3487 0.51 +0.3 3,895,049 + Pf1N1B4_3487 0.51 +1.8 3,895,049 + Pf1N1B4_3487 0.51 +0.6 3,895,049 + Pf1N1B4_3487 0.51 +2.1 3,895,049 + Pf1N1B4_3487 0.51 +1.4 3,895,049 + Pf1N1B4_3487 0.51 +1.5 3,895,049 + Pf1N1B4_3487 0.51 +1.4 3,895,049 + Pf1N1B4_3487 0.51 -0.8 3,895,049 + Pf1N1B4_3487 0.51 +1.7 3,895,049 + Pf1N1B4_3487 0.51 +0.3 3,895,049 + Pf1N1B4_3487 0.51 +0.5 3,895,049 + Pf1N1B4_3487 0.51 +1.5 3,895,049 + Pf1N1B4_3487 0.51 +1.3 3,895,049 + Pf1N1B4_3487 0.51 +0.2 3,895,049 + Pf1N1B4_3487 0.51 +1.7 3,895,049 + Pf1N1B4_3487 0.51 +2.2 3,895,050 - +0.9 3,895,050 - Pf1N1B4_3487 0.51 +1.4 3,895,050 - Pf1N1B4_3487 0.51 -1.4 3,895,050 - Pf1N1B4_3487 0.51 +0.9 3,895,133 + +1.3 3,895,283 - Pf1N1B4_3487 0.69 +0.9 3,895,283 - Pf1N1B4_3487 0.69 -1.1 3,895,292 + Pf1N1B4_3487 0.69 +1.7 3,895,292 + Pf1N1B4_3487 0.69 -1.1 3,895,293 - Pf1N1B4_3487 0.70 -0.1 3,895,293 - Pf1N1B4_3487 0.70 -0.1 3,895,333 + Pf1N1B4_3487 0.73 -1.2 3,895,334 - Pf1N1B4_3487 0.73 -0.6 3,895,334 - Pf1N1B4_3487 0.73 -0.6 3,895,650 + -0.1 3,895,651 - +4.1 3,895,697 + -2.6 3,895,697 + -0.5 3,895,697 + -1.1 3,895,697 + -0.6 3,895,697 + -0.1 3,895,697 + -1.1 3,895,697 + +0.9 3,895,697 + -1.7 3,895,697 + -0.1 3,895,697 + -2.2 3,895,697 + -1.1 3,895,698 - +0.9 3,895,698 - -0.3 3,895,698 - +0.2 3,895,698 - +1.8 3,895,698 - -2.1 3,895,698 - +0.9 3,895,701 + +1.7 3,895,702 - -0.6 3,895,702 - -0.6 3,895,814 + +0.2 3,895,844 + -0.6 3,895,844 + +0.3 3,895,844 + -0.7 3,895,845 - -1.4 3,895,845 - +0.9 3,895,845 - -1.1 3,895,845 - -0.7 3,895,845 - -0.1 3,895,845 - +1.5 3,895,845 - -1.7 3,895,845 - +1.7 3,895,845 - -1.1 3,895,845 - +2.8 3,895,845 - -1.1 3,895,845 - -0.7 3,895,845 - +0.3 3,895,845 - -1.3 3,895,845 - +1.3 3,896,025 + -0.1 3,896,025 + Pf1N1B4_3488 0.15 -0.1 3,896,025 + Pf1N1B4_3488 0.15 +0.5 3,896,202 + Pf1N1B4_3488 0.30 -1.1 3,896,202 + Pf1N1B4_3488 0.30 +0.2 3,896,202 + Pf1N1B4_3488 0.30 +0.2 3,896,202 + Pf1N1B4_3488 0.30 +0.1 3,896,202 + Pf1N1B4_3488 0.30 -1.4 3,896,202 + -0.1 3,896,202 + Pf1N1B4_3488 0.30 +2.5 3,896,202 + Pf1N1B4_3488 0.30 -0.2 3,896,202 + Pf1N1B4_3488 0.30 +0.9 3,896,203 - Pf1N1B4_3488 0.30 -1.1 3,896,203 - Pf1N1B4_3488 0.30 -0.7 3,896,203 - Pf1N1B4_3488 0.30 -0.1 3,896,238 + Pf1N1B4_3488 0.33 -1.7 3,896,238 + Pf1N1B4_3488 0.33 +2.3 3,896,239 - Pf1N1B4_3488 0.33 -0.1 3,896,239 - Pf1N1B4_3488 0.33 -0.4 3,896,268 + Pf1N1B4_3488 0.36 +0.7 3,896,269 - Pf1N1B4_3488 0.36 -0.1 3,896,269 - Pf1N1B4_3488 0.36 -1.4 3,896,295 + Pf1N1B4_3488 0.38 -0.2 3,896,296 - Pf1N1B4_3488 0.38 -0.1 3,896,548 - Pf1N1B4_3488 0.59 -1.7 3,896,548 - Pf1N1B4_3488 0.59 -2.2 3,896,548 - Pf1N1B4_3488 0.59 -1.9 3,896,553 + Pf1N1B4_3488 0.60 -0.1 3,896,553 + Pf1N1B4_3488 0.60 -2.2 3,896,553 + +0.9 3,896,554 - Pf1N1B4_3488 0.60 -0.3 3,896,556 + Pf1N1B4_3488 0.60 -0.3 3,896,556 + Pf1N1B4_3488 0.60 -0.1 3,896,724 + Pf1N1B4_3488 0.74 -0.7 3,896,785 + +2.5 3,896,785 + Pf1N1B4_3488 0.80 -0.9 3,896,785 + Pf1N1B4_3488 0.80 -0.1 3,896,785 + Pf1N1B4_3488 0.80 -0.1 3,896,785 + Pf1N1B4_3488 0.80 -1.0 3,896,786 - Pf1N1B4_3488 0.80 -0.3 3,896,786 - Pf1N1B4_3488 0.80 +0.7 3,896,786 - +0.9 3,897,001 + +1.3 3,897,020 + +1.5 3,897,020 + +0.4 3,897,020 + -0.1 3,897,082 + +0.2 3,897,165 - -0.9 3,897,165 - +0.4 3,897,165 - -0.1 3,897,296 + Pf1N1B4_3489 0.27 +1.5 3,897,296 + Pf1N1B4_3489 0.27 -0.3 3,897,297 - Pf1N1B4_3489 0.27 -0.1 3,897,297 - Pf1N1B4_3489 0.27 +0.9 3,897,297 - Pf1N1B4_3489 0.27 -1.7 3,897,297 - Pf1N1B4_3489 0.27 +1.9 3,897,297 - Pf1N1B4_3489 0.27 -1.4 3,897,297 - Pf1N1B4_3489 0.27 -0.5 3,897,303 - Pf1N1B4_3489 0.28 -1.1 3,897,303 - Pf1N1B4_3489 0.28 -1.4 3,897,332 + Pf1N1B4_3489 0.34 +0.8 3,897,332 + Pf1N1B4_3489 0.34 +0.1 3,897,332 + Pf1N1B4_3489 0.34 +0.7 3,897,333 - Pf1N1B4_3489 0.34 -1.8 3,897,333 - +1.5 3,897,333 - Pf1N1B4_3489 0.34 +0.9 3,897,362 + Pf1N1B4_3489 0.40 +0.9 3,897,758 + +1.5 3,897,758 + -1.4 3,897,758 + -1.4 3,897,758 + -1.7 3,897,817 + -0.2 3,897,817 + -0.3 3,897,818 - -0.7 3,897,818 - -2.8 3,897,818 - +0.5 3,897,818 - -1.8 3,897,818 - +0.3 3,897,818 - +0.5 3,897,825 - +0.5 3,897,826 + -1.4 3,897,970 + Pf1N1B4_3490 0.26 +1.3 3,897,970 + Pf1N1B4_3490 0.26 -0.7 3,897,970 + Pf1N1B4_3490 0.26 -0.5 3,897,970 + Pf1N1B4_3490 0.26 -0.4 3,897,970 + Pf1N1B4_3490 0.26 -2.2 3,897,970 + Pf1N1B4_3490 0.26 -0.1 3,897,970 + Pf1N1B4_3490 0.26 +0.8 3,897,970 + -0.1 3,897,971 - Pf1N1B4_3490 0.26 -0.6 3,897,971 - Pf1N1B4_3490 0.26 -2.1 3,897,971 - Pf1N1B4_3490 0.26 +0.6 3,897,971 - Pf1N1B4_3490 0.26 -1.9 3,897,979 + Pf1N1B4_3490 0.27 -0.1 3,897,979 + Pf1N1B4_3490 0.27 +0.0 3,897,979 + -1.1 3,897,980 - Pf1N1B4_3490 0.27 -0.1 3,897,989 - Pf1N1B4_3490 0.29 -1.4 3,897,989 - Pf1N1B4_3490 0.29 -1.1
Or see this region's nucleotide sequence