Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3413 and Pf1N1B4_3414 are separated by 6 nucleotides Pf1N1B4_3414 and Pf1N1B4_3415 are separated by 141 nucleotides Pf1N1B4_3415 and Pf1N1B4_3416 are separated by 215 nucleotides
Pf1N1B4_3413 - Transcriptional regulator, LysR family, at 3,803,774 to 3,804,652
_3413
Pf1N1B4_3414 - PROBABLE MEMBRANE PROTEIN, at 3,804,659 to 3,804,907
_3414
Pf1N1B4_3415 - Transcriptional regulator, TetR family, at 3,805,049 to 3,805,657
_3415
Pf1N1B4_3416 - Outer membrane protein romA, at 3,805,873 to 3,806,931
_3416
Position (kb)
3805
3806 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3804.308 kb on + strand, within Pf1N1B4_3413 at 3804.308 kb on + strand, within Pf1N1B4_3413 at 3804.439 kb on + strand, within Pf1N1B4_3413 at 3804.465 kb on + strand, within Pf1N1B4_3413 at 3804.465 kb on + strand at 3804.817 kb on + strand, within Pf1N1B4_3414 at 3804.818 kb on - strand, within Pf1N1B4_3414 at 3804.818 kb on - strand, within Pf1N1B4_3414 at 3804.818 kb on - strand, within Pf1N1B4_3414 at 3804.944 kb on + strand at 3804.945 kb on - strand at 3804.946 kb on + strand at 3804.947 kb on - strand at 3804.990 kb on + strand at 3804.991 kb on - strand at 3804.991 kb on - strand at 3804.995 kb on - strand at 3805.060 kb on - strand at 3805.060 kb on - strand at 3805.248 kb on - strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.528 kb on + strand, within Pf1N1B4_3415 at 3805.657 kb on + strand at 3805.657 kb on + strand at 3805.661 kb on + strand at 3805.661 kb on + strand at 3805.661 kb on + strand at 3805.704 kb on - strand at 3805.737 kb on - strand at 3805.737 kb on - strand at 3805.750 kb on + strand at 3805.761 kb on + strand at 3805.818 kb on + strand at 3805.819 kb on - strand at 3805.819 kb on - strand at 3805.884 kb on - strand at 3805.955 kb on + strand at 3805.956 kb on - strand at 3806.127 kb on - strand, within Pf1N1B4_3416 at 3806.127 kb on - strand, within Pf1N1B4_3416 at 3806.284 kb on + strand, within Pf1N1B4_3416 at 3806.284 kb on + strand, within Pf1N1B4_3416 at 3806.284 kb on + strand, within Pf1N1B4_3416 at 3806.285 kb on - strand, within Pf1N1B4_3416 at 3806.285 kb on - strand, within Pf1N1B4_3416 at 3806.285 kb on - strand, within Pf1N1B4_3416 at 3806.335 kb on + strand, within Pf1N1B4_3416 at 3806.336 kb on - strand, within Pf1N1B4_3416 at 3806.641 kb on + strand, within Pf1N1B4_3416 at 3806.641 kb on + strand, within Pf1N1B4_3416 at 3806.642 kb on - strand, within Pf1N1B4_3416
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 3,804,308 + Pf1N1B4_3413 0.61 -2.4 3,804,308 + Pf1N1B4_3413 0.61 -2.3 3,804,439 + Pf1N1B4_3413 0.76 +1.3 3,804,465 + Pf1N1B4_3413 0.79 -0.4 3,804,465 + +1.4 3,804,817 + Pf1N1B4_3414 0.63 -0.6 3,804,818 - Pf1N1B4_3414 0.64 +0.4 3,804,818 - Pf1N1B4_3414 0.64 +0.7 3,804,818 - Pf1N1B4_3414 0.64 +0.7 3,804,944 + +0.6 3,804,945 - -1.0 3,804,946 + -0.2 3,804,947 - +3.3 3,804,990 + +1.0 3,804,991 - +0.5 3,804,991 - +0.0 3,804,995 - +0.7 3,805,060 - -0.7 3,805,060 - +0.0 3,805,248 - Pf1N1B4_3415 0.33 +1.4 3,805,515 + Pf1N1B4_3415 0.77 +0.6 3,805,515 + Pf1N1B4_3415 0.77 +0.4 3,805,515 + Pf1N1B4_3415 0.77 +1.3 3,805,516 - Pf1N1B4_3415 0.77 +1.6 3,805,516 - Pf1N1B4_3415 0.77 +0.3 3,805,516 - Pf1N1B4_3415 0.77 -0.3 3,805,528 + Pf1N1B4_3415 0.79 -0.6 3,805,657 + -1.3 3,805,657 + +0.4 3,805,661 + +0.3 3,805,661 + +1.6 3,805,661 + +1.7 3,805,704 - -2.4 3,805,737 - -0.6 3,805,737 - -0.3 3,805,750 + -2.6 3,805,761 + +0.7 3,805,818 + +0.7 3,805,819 - +1.0 3,805,819 - -1.3 3,805,884 - +1.0 3,805,955 + +1.0 3,805,956 - +0.1 3,806,127 - Pf1N1B4_3416 0.24 -0.4 3,806,127 - Pf1N1B4_3416 0.24 +2.2 3,806,284 + Pf1N1B4_3416 0.39 +0.6 3,806,284 + Pf1N1B4_3416 0.39 +0.8 3,806,284 + Pf1N1B4_3416 0.39 -1.3 3,806,285 - Pf1N1B4_3416 0.39 -1.4 3,806,285 - Pf1N1B4_3416 0.39 +0.0 3,806,285 - Pf1N1B4_3416 0.39 -1.6 3,806,335 + Pf1N1B4_3416 0.44 +0.3 3,806,336 - Pf1N1B4_3416 0.44 +2.0 3,806,641 + Pf1N1B4_3416 0.73 -1.2 3,806,641 + Pf1N1B4_3416 0.73 +2.8 3,806,642 - Pf1N1B4_3416 0.73 +0.0
Or see this region's nucleotide sequence