Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3394 and Pf1N1B4_3395 are separated by 270 nucleotides Pf1N1B4_3395 and Pf1N1B4_3396 are separated by 18 nucleotides Pf1N1B4_3396 and Pf1N1B4_3397 are separated by 141 nucleotides Pf1N1B4_3397 and Pf1N1B4_3398 are separated by 100 nucleotides
Pf1N1B4_3394 - Aquaporin Z, at 3,787,964 to 3,788,662
_3394
Pf1N1B4_3395 - Phosphohydrolase (MutT/nudix family protein), at 3,788,933 to 3,789,538
_3395
Pf1N1B4_3396 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,789,557 to 3,790,042
_3396
Pf1N1B4_3397 - Ketosteroid isomerase homolog, at 3,790,184 to 3,790,609
_3397
Pf1N1B4_3398 - DGPFAETKE, at 3,790,710 to 3,791,054
_3398
Position (kb)
3789
3790
3791 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3788.662 kb on + strand at 3788.663 kb on - strand at 3788.663 kb on - strand at 3788.663 kb on - strand at 3788.683 kb on + strand at 3788.683 kb on + strand at 3788.683 kb on + strand at 3788.684 kb on - strand at 3788.715 kb on + strand at 3788.715 kb on + strand at 3788.715 kb on + strand at 3788.716 kb on - strand at 3788.736 kb on + strand at 3788.769 kb on + strand at 3788.769 kb on + strand at 3788.769 kb on + strand at 3788.793 kb on - strand at 3788.793 kb on - strand at 3788.820 kb on + strand at 3788.821 kb on - strand at 3788.821 kb on - strand at 3788.821 kb on - strand at 3788.938 kb on - strand at 3788.945 kb on - strand at 3788.976 kb on + strand at 3788.976 kb on + strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3788.977 kb on - strand at 3789.447 kb on + strand, within Pf1N1B4_3395 at 3789.504 kb on + strand at 3789.504 kb on + strand at 3789.505 kb on - strand at 3789.603 kb on + strand at 3789.603 kb on + strand at 3789.603 kb on + strand at 3789.604 kb on - strand at 3789.604 kb on - strand at 3789.604 kb on - strand at 3789.604 kb on - strand at 3789.630 kb on + strand, within Pf1N1B4_3396 at 3789.631 kb on - strand, within Pf1N1B4_3396 at 3789.639 kb on + strand, within Pf1N1B4_3396 at 3789.639 kb on + strand, within Pf1N1B4_3396 at 3789.640 kb on - strand, within Pf1N1B4_3396 at 3790.021 kb on - strand at 3790.110 kb on + strand at 3790.146 kb on + strand at 3790.223 kb on + strand at 3790.224 kb on - strand at 3790.334 kb on + strand, within Pf1N1B4_3397 at 3790.660 kb on + strand at 3790.660 kb on + strand at 3790.660 kb on + strand at 3790.661 kb on - strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.716 kb on + strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.717 kb on - strand at 3790.720 kb on + strand at 3790.720 kb on + strand at 3790.720 kb on + strand at 3790.720 kb on + strand at 3790.720 kb on + strand at 3790.721 kb on - strand at 3790.721 kb on - strand at 3790.721 kb on - strand at 3790.731 kb on + strand at 3790.731 kb on + strand at 3790.731 kb on + strand at 3790.731 kb on + strand at 3790.732 kb on - strand at 3790.732 kb on - strand at 3790.734 kb on - strand at 3790.734 kb on - strand at 3790.734 kb on - strand at 3790.944 kb on + strand, within Pf1N1B4_3398
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 3,788,662 + +2.3 3,788,663 - +0.9 3,788,663 - +0.4 3,788,663 - -0.7 3,788,683 + +0.0 3,788,683 + -0.3 3,788,683 + -1.8 3,788,684 - -1.3 3,788,715 + -1.8 3,788,715 + -0.2 3,788,715 + +2.4 3,788,716 - +0.6 3,788,736 + -1.1 3,788,769 + +1.3 3,788,769 + +1.0 3,788,769 + +0.2 3,788,793 - +0.4 3,788,793 - +1.0 3,788,820 + -0.2 3,788,821 - -1.6 3,788,821 - -0.9 3,788,821 - +1.2 3,788,938 - -1.6 3,788,945 - +0.3 3,788,976 + -0.3 3,788,976 + +3.3 3,788,977 - +0.4 3,788,977 - +0.8 3,788,977 - +0.3 3,788,977 - +0.0 3,788,977 - -0.6 3,788,977 - -1.3 3,788,977 - -2.1 3,788,977 - +0.5 3,789,447 + Pf1N1B4_3395 0.85 +2.6 3,789,504 + -0.1 3,789,504 + +0.0 3,789,505 - -0.8 3,789,603 + -0.8 3,789,603 + +0.4 3,789,603 + -1.1 3,789,604 - -0.6 3,789,604 - +0.0 3,789,604 - +0.0 3,789,604 - -1.1 3,789,630 + Pf1N1B4_3396 0.15 +0.9 3,789,631 - Pf1N1B4_3396 0.15 -2.1 3,789,639 + Pf1N1B4_3396 0.17 -1.6 3,789,639 + Pf1N1B4_3396 0.17 -1.1 3,789,640 - Pf1N1B4_3396 0.17 -1.6 3,790,021 - -0.3 3,790,110 + +0.0 3,790,146 + -1.6 3,790,223 + +1.0 3,790,224 - -0.6 3,790,334 + Pf1N1B4_3397 0.35 +1.3 3,790,660 + +0.7 3,790,660 + -1.1 3,790,660 + +0.3 3,790,661 - -1.0 3,790,716 + +0.3 3,790,716 + +0.2 3,790,716 + +0.0 3,790,716 + -0.1 3,790,716 + -0.3 3,790,716 + +0.3 3,790,716 + -1.3 3,790,716 + +2.0 3,790,717 - +0.0 3,790,717 - +0.2 3,790,717 - +0.7 3,790,717 - +0.0 3,790,717 - +2.0 3,790,717 - -0.6 3,790,717 - +0.6 3,790,717 - +1.3 3,790,717 - -2.6 3,790,720 + +0.0 3,790,720 + +0.3 3,790,720 + -2.3 3,790,720 + -0.2 3,790,720 + +1.0 3,790,721 - -1.3 3,790,721 - +1.4 3,790,721 - +1.8 3,790,731 + +0.0 3,790,731 + -0.7 3,790,731 + +1.4 3,790,731 + +2.0 3,790,732 - -0.0 3,790,732 - -1.1 3,790,734 - +1.3 3,790,734 - +1.3 3,790,734 - -0.6 3,790,944 + Pf1N1B4_3398 0.68 +0.0
Or see this region's nucleotide sequence