Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3263 and Pf1N1B4_3264 are separated by 175 nucleotides Pf1N1B4_3264 and Pf1N1B4_3265 are separated by 59 nucleotides
Pf1N1B4_3263 - FIG126011: Hypothetical protein, at 3,653,163 to 3,654,572
_3263
Pf1N1B4_3264 - Methyltransferase (EC 2.1.1.-), at 3,654,748 to 3,655,962
_3264
Pf1N1B4_3265 - Permease of the drug/metabolite transporter (DMT) superfamily, at 3,656,022 to 3,656,942
_3265
Position (kb)
3654
3655
3656 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3653.912 kb on + strand, within Pf1N1B4_3263 at 3653.933 kb on + strand, within Pf1N1B4_3263 at 3653.962 kb on + strand, within Pf1N1B4_3263 at 3654.072 kb on + strand, within Pf1N1B4_3263 at 3654.072 kb on + strand, within Pf1N1B4_3263 at 3654.072 kb on + strand, within Pf1N1B4_3263 at 3654.072 kb on + strand, within Pf1N1B4_3263 at 3654.073 kb on - strand, within Pf1N1B4_3263 at 3654.073 kb on - strand, within Pf1N1B4_3263 at 3654.073 kb on - strand, within Pf1N1B4_3263 at 3654.073 kb on - strand, within Pf1N1B4_3263 at 3654.129 kb on + strand, within Pf1N1B4_3263 at 3654.129 kb on + strand, within Pf1N1B4_3263 at 3654.130 kb on - strand, within Pf1N1B4_3263 at 3654.130 kb on - strand, within Pf1N1B4_3263 at 3654.357 kb on + strand, within Pf1N1B4_3263 at 3654.357 kb on + strand, within Pf1N1B4_3263 at 3654.358 kb on - strand, within Pf1N1B4_3263 at 3654.570 kb on + strand at 3654.669 kb on - strand at 3654.669 kb on - strand at 3654.699 kb on - strand at 3654.703 kb on + strand at 3654.862 kb on + strand at 3654.863 kb on - strand at 3654.949 kb on + strand, within Pf1N1B4_3264 at 3654.951 kb on + strand, within Pf1N1B4_3264 at 3654.951 kb on + strand, within Pf1N1B4_3264 at 3654.952 kb on - strand, within Pf1N1B4_3264 at 3655.043 kb on + strand, within Pf1N1B4_3264 at 3655.384 kb on + strand, within Pf1N1B4_3264 at 3655.384 kb on + strand at 3655.384 kb on + strand, within Pf1N1B4_3264 at 3655.385 kb on - strand, within Pf1N1B4_3264 at 3655.583 kb on - strand, within Pf1N1B4_3264 at 3655.906 kb on + strand at 3655.955 kb on - strand at 3656.020 kb on + strand at 3656.021 kb on - strand at 3656.021 kb on - strand at 3656.021 kb on - strand at 3656.023 kb on + strand at 3656.024 kb on - strand at 3656.139 kb on - strand, within Pf1N1B4_3265 at 3656.139 kb on - strand, within Pf1N1B4_3265 at 3656.294 kb on - strand, within Pf1N1B4_3265 at 3656.294 kb on - strand, within Pf1N1B4_3265 at 3656.294 kb on - strand, within Pf1N1B4_3265 at 3656.351 kb on - strand, within Pf1N1B4_3265 at 3656.351 kb on - strand, within Pf1N1B4_3265 at 3656.530 kb on + strand, within Pf1N1B4_3265 at 3656.612 kb on - strand, within Pf1N1B4_3265 at 3656.612 kb on - strand, within Pf1N1B4_3265 at 3656.892 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 3,653,912 + Pf1N1B4_3263 0.53 +0.7 3,653,933 + Pf1N1B4_3263 0.55 +0.4 3,653,962 + Pf1N1B4_3263 0.57 -0.1 3,654,072 + Pf1N1B4_3263 0.64 -2.1 3,654,072 + Pf1N1B4_3263 0.64 -0.2 3,654,072 + Pf1N1B4_3263 0.64 -0.3 3,654,072 + Pf1N1B4_3263 0.64 +1.0 3,654,073 - Pf1N1B4_3263 0.65 -1.0 3,654,073 - Pf1N1B4_3263 0.65 +0.0 3,654,073 - Pf1N1B4_3263 0.65 +0.0 3,654,073 - Pf1N1B4_3263 0.65 +0.3 3,654,129 + Pf1N1B4_3263 0.69 +0.3 3,654,129 + Pf1N1B4_3263 0.69 +0.4 3,654,130 - Pf1N1B4_3263 0.69 +0.3 3,654,130 - Pf1N1B4_3263 0.69 +1.8 3,654,357 + Pf1N1B4_3263 0.85 -0.3 3,654,357 + Pf1N1B4_3263 0.85 +2.4 3,654,358 - Pf1N1B4_3263 0.85 -2.1 3,654,570 + +1.5 3,654,669 - -1.2 3,654,669 - -1.0 3,654,699 - -1.0 3,654,703 + +2.3 3,654,862 + -2.3 3,654,863 - -3.1 3,654,949 + Pf1N1B4_3264 0.17 -1.3 3,654,951 + Pf1N1B4_3264 0.17 +0.4 3,654,951 + Pf1N1B4_3264 0.17 -1.9 3,654,952 - Pf1N1B4_3264 0.17 -1.0 3,655,043 + Pf1N1B4_3264 0.24 -2.1 3,655,384 + Pf1N1B4_3264 0.52 -2.1 3,655,384 + +0.6 3,655,384 + Pf1N1B4_3264 0.52 -0.7 3,655,385 - Pf1N1B4_3264 0.52 +0.6 3,655,583 - Pf1N1B4_3264 0.69 +1.0 3,655,906 + -0.3 3,655,955 - -1.3 3,656,020 + -0.1 3,656,021 - +0.1 3,656,021 - -2.1 3,656,021 - +1.0 3,656,023 + -0.1 3,656,024 - -0.1 3,656,139 - Pf1N1B4_3265 0.13 -0.2 3,656,139 - Pf1N1B4_3265 0.13 +1.6 3,656,294 - Pf1N1B4_3265 0.30 +1.0 3,656,294 - Pf1N1B4_3265 0.30 -1.6 3,656,294 - Pf1N1B4_3265 0.30 +0.6 3,656,351 - Pf1N1B4_3265 0.36 +1.6 3,656,351 - Pf1N1B4_3265 0.36 -1.3 3,656,530 + Pf1N1B4_3265 0.55 +0.2 3,656,612 - Pf1N1B4_3265 0.64 +1.6 3,656,612 - Pf1N1B4_3265 0.64 -1.0 3,656,892 - +1.9
Or see this region's nucleotide sequence