Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3245 and Pf1N1B4_3246 overlap by 1 nucleotides Pf1N1B4_3246 and Pf1N1B4_3247 are separated by 3 nucleotides
Pf1N1B4_3245 - RND efflux system, outer membrane lipoprotein, NodT family, at 3,632,295 to 3,633,755
_3245
Pf1N1B4_3246 - RND efflux system, inner membrane transporter CmeB, at 3,633,755 to 3,636,916
_3246
Pf1N1B4_3247 - Multidrug efflux RND membrane fusion protein MexC, at 3,636,920 to 3,638,077
_3247
Position (kb)
3633
3634
3635
3636
3637 Strain fitness (log2 ratio)
-2
-1
0
1 at 3633.143 kb on - strand, within Pf1N1B4_3245 at 3633.215 kb on - strand, within Pf1N1B4_3245 at 3633.281 kb on - strand, within Pf1N1B4_3245 at 3633.281 kb on - strand, within Pf1N1B4_3245 at 3633.338 kb on - strand, within Pf1N1B4_3245 at 3633.770 kb on - strand at 3633.770 kb on - strand at 3633.770 kb on - strand at 3634.037 kb on - strand at 3634.132 kb on - strand, within Pf1N1B4_3246 at 3634.318 kb on - strand, within Pf1N1B4_3246 at 3634.442 kb on + strand, within Pf1N1B4_3246 at 3634.594 kb on - strand, within Pf1N1B4_3246 at 3634.594 kb on - strand, within Pf1N1B4_3246 at 3634.635 kb on + strand, within Pf1N1B4_3246 at 3634.636 kb on - strand, within Pf1N1B4_3246 at 3634.640 kb on - strand at 3634.640 kb on - strand, within Pf1N1B4_3246 at 3635.296 kb on - strand, within Pf1N1B4_3246 at 3635.316 kb on - strand, within Pf1N1B4_3246 at 3635.316 kb on - strand, within Pf1N1B4_3246 at 3635.316 kb on - strand, within Pf1N1B4_3246 at 3635.793 kb on - strand, within Pf1N1B4_3246 at 3636.791 kb on - strand at 3636.947 kb on - strand at 3636.947 kb on - strand at 3636.947 kb on - strand at 3636.950 kb on - strand at 3636.950 kb on - strand at 3636.950 kb on - strand at 3636.950 kb on - strand at 3636.950 kb on - strand at 3636.950 kb on - strand at 3637.492 kb on - strand, within Pf1N1B4_3247 at 3637.492 kb on - strand, within Pf1N1B4_3247 at 3637.600 kb on - strand at 3637.786 kb on - strand, within Pf1N1B4_3247
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 3,633,143 - Pf1N1B4_3245 0.58 +0.6 3,633,215 - Pf1N1B4_3245 0.63 -0.5 3,633,281 - Pf1N1B4_3245 0.67 -1.3 3,633,281 - Pf1N1B4_3245 0.67 -1.9 3,633,338 - Pf1N1B4_3245 0.71 -1.8 3,633,770 - -1.8 3,633,770 - -0.5 3,633,770 - +0.1 3,634,037 - -2.1 3,634,132 - Pf1N1B4_3246 0.12 -1.1 3,634,318 - Pf1N1B4_3246 0.18 -2.3 3,634,442 + Pf1N1B4_3246 0.22 -1.8 3,634,594 - Pf1N1B4_3246 0.27 -0.9 3,634,594 - Pf1N1B4_3246 0.27 +1.1 3,634,635 + Pf1N1B4_3246 0.28 -0.3 3,634,636 - Pf1N1B4_3246 0.28 -1.9 3,634,640 - -0.9 3,634,640 - Pf1N1B4_3246 0.28 -0.3 3,635,296 - Pf1N1B4_3246 0.49 -1.3 3,635,316 - Pf1N1B4_3246 0.49 -2.3 3,635,316 - Pf1N1B4_3246 0.49 -1.8 3,635,316 - Pf1N1B4_3246 0.49 +1.1 3,635,793 - Pf1N1B4_3246 0.64 -0.9 3,636,791 - -0.4 3,636,947 - -0.5 3,636,947 - -0.1 3,636,947 - -2.3 3,636,950 - +0.1 3,636,950 - +0.1 3,636,950 - -2.3 3,636,950 - -1.0 3,636,950 - +0.6 3,636,950 - +0.5 3,637,492 - Pf1N1B4_3247 0.49 -1.9 3,637,492 - Pf1N1B4_3247 0.49 -2.3 3,637,600 - -0.8 3,637,786 - Pf1N1B4_3247 0.75 -2.3
Or see this region's nucleotide sequence