Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3077 and Pf1N1B4_3078 are separated by 33 nucleotides Pf1N1B4_3078 and Pf1N1B4_3079 are separated by 265 nucleotides
Pf1N1B4_3077 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 3,459,950 to 3,460,984
_3077
Pf1N1B4_3078 - FIG138056: a glutathione-dependent thiol reductase, at 3,461,018 to 3,461,380
_3078
Pf1N1B4_3079 - Transcription-repair coupling factor (superfamily II helicase), at 3,461,646 to 3,462,377
_3079
Position (kb)
3461
3462 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3460.997 kb on - strand at 3461.020 kb on - strand at 3461.020 kb on - strand at 3461.020 kb on - strand at 3461.038 kb on - strand at 3461.038 kb on - strand at 3461.038 kb on - strand at 3461.038 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.042 kb on - strand at 3461.269 kb on - strand, within Pf1N1B4_3078 at 3461.269 kb on - strand, within Pf1N1B4_3078 at 3461.269 kb on - strand, within Pf1N1B4_3078 at 3461.269 kb on - strand, within Pf1N1B4_3078 at 3461.284 kb on - strand, within Pf1N1B4_3078 at 3461.284 kb on - strand, within Pf1N1B4_3078 at 3461.410 kb on + strand at 3461.411 kb on - strand at 3461.411 kb on - strand at 3461.411 kb on - strand at 3461.425 kb on + strand at 3461.426 kb on - strand at 3461.426 kb on - strand at 3461.473 kb on + strand at 3461.473 kb on + strand at 3461.559 kb on - strand at 3461.559 kb on - strand at 3461.559 kb on - strand at 3461.591 kb on + strand at 3461.591 kb on + strand at 3461.591 kb on + strand at 3461.592 kb on - strand at 3461.592 kb on - strand at 3461.592 kb on - strand at 3461.691 kb on - strand at 3461.777 kb on - strand, within Pf1N1B4_3079 at 3461.777 kb on - strand, within Pf1N1B4_3079 at 3461.784 kb on - strand, within Pf1N1B4_3079 at 3461.785 kb on + strand, within Pf1N1B4_3079 at 3461.785 kb on + strand, within Pf1N1B4_3079 at 3461.785 kb on + strand, within Pf1N1B4_3079 at 3461.785 kb on + strand, within Pf1N1B4_3079 at 3461.786 kb on - strand, within Pf1N1B4_3079 at 3461.786 kb on - strand, within Pf1N1B4_3079 at 3461.786 kb on - strand, within Pf1N1B4_3079 at 3461.879 kb on - strand, within Pf1N1B4_3079 at 3461.879 kb on - strand, within Pf1N1B4_3079 at 3461.879 kb on - strand, within Pf1N1B4_3079 at 3461.891 kb on + strand, within Pf1N1B4_3079 at 3462.065 kb on + strand, within Pf1N1B4_3079 at 3462.065 kb on + strand, within Pf1N1B4_3079 at 3462.065 kb on + strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.066 kb on - strand, within Pf1N1B4_3079 at 3462.069 kb on - strand, within Pf1N1B4_3079 at 3462.134 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.136 kb on + strand, within Pf1N1B4_3079 at 3462.137 kb on - strand, within Pf1N1B4_3079 at 3462.200 kb on + strand, within Pf1N1B4_3079 at 3462.312 kb on - strand at 3462.322 kb on + strand at 3462.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 3,460,997 - +0.0 3,461,020 - +0.3 3,461,020 - +1.1 3,461,020 - +1.0 3,461,038 - -1.1 3,461,038 - -0.4 3,461,038 - +1.1 3,461,038 - -1.2 3,461,042 - -0.9 3,461,042 - -0.3 3,461,042 - -0.6 3,461,042 - +1.1 3,461,042 - -0.2 3,461,042 - +0.8 3,461,042 - +0.5 3,461,269 - Pf1N1B4_3078 0.69 -2.0 3,461,269 - Pf1N1B4_3078 0.69 -0.9 3,461,269 - Pf1N1B4_3078 0.69 -0.1 3,461,269 - Pf1N1B4_3078 0.69 +0.8 3,461,284 - Pf1N1B4_3078 0.73 +0.1 3,461,284 - Pf1N1B4_3078 0.73 -0.6 3,461,410 + -1.0 3,461,411 - +1.1 3,461,411 - +1.1 3,461,411 - +0.1 3,461,425 + -0.9 3,461,426 - +0.4 3,461,426 - -1.0 3,461,473 + -0.9 3,461,473 + +0.2 3,461,559 - -0.3 3,461,559 - -0.4 3,461,559 - -1.7 3,461,591 + -0.1 3,461,591 + +1.4 3,461,591 + -0.3 3,461,592 - +0.8 3,461,592 - -0.6 3,461,592 - +0.4 3,461,691 - -1.7 3,461,777 - Pf1N1B4_3079 0.18 +1.6 3,461,777 - Pf1N1B4_3079 0.18 -0.3 3,461,784 - Pf1N1B4_3079 0.19 +0.4 3,461,785 + Pf1N1B4_3079 0.19 -1.5 3,461,785 + Pf1N1B4_3079 0.19 +2.1 3,461,785 + Pf1N1B4_3079 0.19 +1.6 3,461,785 + Pf1N1B4_3079 0.19 -0.4 3,461,786 - Pf1N1B4_3079 0.19 -1.1 3,461,786 - Pf1N1B4_3079 0.19 +1.1 3,461,786 - Pf1N1B4_3079 0.19 -0.1 3,461,879 - Pf1N1B4_3079 0.32 -1.4 3,461,879 - Pf1N1B4_3079 0.32 -0.9 3,461,879 - Pf1N1B4_3079 0.32 -0.1 3,461,891 + Pf1N1B4_3079 0.33 +2.5 3,462,065 + Pf1N1B4_3079 0.57 +0.8 3,462,065 + Pf1N1B4_3079 0.57 +0.7 3,462,065 + Pf1N1B4_3079 0.57 +1.1 3,462,066 - Pf1N1B4_3079 0.57 -0.7 3,462,066 - Pf1N1B4_3079 0.57 +1.6 3,462,066 - Pf1N1B4_3079 0.57 -0.7 3,462,066 - Pf1N1B4_3079 0.57 +2.3 3,462,066 - Pf1N1B4_3079 0.57 +0.1 3,462,066 - Pf1N1B4_3079 0.57 +2.7 3,462,066 - Pf1N1B4_3079 0.57 -0.1 3,462,066 - Pf1N1B4_3079 0.57 -0.1 3,462,069 - Pf1N1B4_3079 0.58 -1.7 3,462,134 + Pf1N1B4_3079 0.67 -0.1 3,462,136 + Pf1N1B4_3079 0.67 +0.8 3,462,136 + Pf1N1B4_3079 0.67 +0.3 3,462,136 + Pf1N1B4_3079 0.67 -0.1 3,462,136 + Pf1N1B4_3079 0.67 -0.3 3,462,136 + Pf1N1B4_3079 0.67 -0.9 3,462,137 - Pf1N1B4_3079 0.67 +0.9 3,462,200 + Pf1N1B4_3079 0.76 -1.4 3,462,312 - +0.6 3,462,322 + -0.2 3,462,378 + +1.1
Or see this region's nucleotide sequence