Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2859 and Pf1N1B4_2860 overlap by 11 nucleotides Pf1N1B4_2860 and Pf1N1B4_2861 are separated by 77 nucleotides Pf1N1B4_2861 and Pf1N1B4_2862 are separated by 207 nucleotides Pf1N1B4_2862 and Pf1N1B4_2863 are separated by 334 nucleotides
Pf1N1B4_2859 - Two-component sensor CbrA: intrcellular carbon:nitrogen balance, at 3,206,341 to 3,209,289
_2859
Pf1N1B4_2860 - FIG00954153: hypothetical protein, at 3,209,279 to 3,209,455
_2860
Pf1N1B4_2861 - glutamyl-Q-tRNA synthetase, at 3,209,533 to 3,210,429
_2861
Pf1N1B4_2862 - C4-type zinc finger protein, DksA/TraR family, at 3,210,637 to 3,211,077
_2862
Pf1N1B4_2863 - Valine--pyruvate aminotransferase (EC 2.6.1.66), at 3,211,412 to 3,212,584
_2863
Position (kb)
3209
3210
3211 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3208.670 kb on + strand, within Pf1N1B4_2859 at 3208.670 kb on + strand, within Pf1N1B4_2859 at 3208.671 kb on - strand, within Pf1N1B4_2859 at 3208.671 kb on - strand, within Pf1N1B4_2859 at 3208.671 kb on - strand, within Pf1N1B4_2859 at 3208.679 kb on + strand, within Pf1N1B4_2859 at 3208.680 kb on - strand, within Pf1N1B4_2859 at 3208.680 kb on - strand, within Pf1N1B4_2859 at 3208.803 kb on + strand, within Pf1N1B4_2859 at 3209.000 kb on + strand at 3209.000 kb on + strand at 3209.000 kb on + strand at 3209.001 kb on - strand at 3209.001 kb on - strand at 3209.072 kb on + strand at 3209.072 kb on + strand at 3209.072 kb on + strand at 3209.072 kb on + strand at 3209.072 kb on + strand at 3209.102 kb on + strand at 3209.103 kb on - strand at 3209.144 kb on + strand at 3209.145 kb on - strand at 3209.145 kb on - strand at 3209.145 kb on - strand at 3209.165 kb on + strand at 3209.165 kb on + strand at 3209.165 kb on + strand at 3209.165 kb on + strand at 3209.165 kb on + strand at 3209.165 kb on + strand at 3209.165 kb on + strand at 3209.165 kb on + strand at 3209.166 kb on - strand at 3209.166 kb on - strand at 3209.166 kb on - strand at 3209.166 kb on - strand at 3209.166 kb on - strand at 3209.246 kb on + strand at 3209.349 kb on + strand, within Pf1N1B4_2860 at 3209.349 kb on + strand, within Pf1N1B4_2860 at 3209.349 kb on + strand, within Pf1N1B4_2860 at 3209.349 kb on + strand, within Pf1N1B4_2860 at 3209.349 kb on + strand, within Pf1N1B4_2860 at 3209.349 kb on + strand, within Pf1N1B4_2860 at 3209.349 kb on + strand, within Pf1N1B4_2860 at 3209.349 kb on + strand, within Pf1N1B4_2860 at 3209.349 kb on + strand, within Pf1N1B4_2860 at 3209.349 kb on + strand, within Pf1N1B4_2860 at 3209.349 kb on + strand, within Pf1N1B4_2860 at 3209.349 kb on + strand, within Pf1N1B4_2860 at 3209.350 kb on - strand, within Pf1N1B4_2860 at 3209.350 kb on - strand, within Pf1N1B4_2860 at 3209.350 kb on - strand, within Pf1N1B4_2860 at 3209.350 kb on - strand, within Pf1N1B4_2860 at 3209.350 kb on - strand, within Pf1N1B4_2860 at 3209.350 kb on - strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand, within Pf1N1B4_2860 at 3209.412 kb on + strand at 3209.423 kb on + strand, within Pf1N1B4_2860 at 3209.423 kb on + strand, within Pf1N1B4_2860 at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.445 kb on + strand at 3209.446 kb on - strand at 3209.446 kb on - strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.451 kb on + strand at 3209.452 kb on - strand at 3209.452 kb on - strand at 3209.452 kb on - strand at 3209.452 kb on - strand at 3209.452 kb on - strand at 3209.452 kb on - strand at 3209.452 kb on - strand at 3209.452 kb on - strand at 3209.452 kb on - strand at 3209.452 kb on - strand at 3209.452 kb on - strand at 3209.452 kb on - strand at 3209.472 kb on + strand at 3209.680 kb on + strand, within Pf1N1B4_2861 at 3209.813 kb on + strand, within Pf1N1B4_2861 at 3209.813 kb on + strand, within Pf1N1B4_2861 at 3209.907 kb on - strand, within Pf1N1B4_2861 at 3210.002 kb on + strand, within Pf1N1B4_2861 at 3210.002 kb on + strand, within Pf1N1B4_2861 at 3210.002 kb on + strand, within Pf1N1B4_2861 at 3210.077 kb on + strand, within Pf1N1B4_2861 at 3210.077 kb on + strand, within Pf1N1B4_2861 at 3210.077 kb on + strand, within Pf1N1B4_2861 at 3210.077 kb on + strand, within Pf1N1B4_2861 at 3210.077 kb on + strand at 3210.167 kb on + strand, within Pf1N1B4_2861 at 3210.167 kb on + strand, within Pf1N1B4_2861 at 3210.168 kb on - strand, within Pf1N1B4_2861 at 3210.168 kb on - strand, within Pf1N1B4_2861 at 3210.367 kb on + strand at 3210.368 kb on - strand at 3210.368 kb on - strand at 3210.368 kb on - strand at 3210.385 kb on - strand at 3210.404 kb on + strand at 3210.429 kb on + strand at 3210.429 kb on + strand at 3210.430 kb on - strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.441 kb on + strand at 3210.442 kb on - strand at 3210.442 kb on - strand at 3210.442 kb on - strand at 3210.442 kb on - strand at 3210.513 kb on + strand at 3210.638 kb on + strand at 3210.639 kb on - strand at 3210.639 kb on - strand at 3210.639 kb on - strand at 3210.639 kb on - strand at 3210.639 kb on - strand at 3211.102 kb on - strand at 3211.102 kb on - strand at 3211.102 kb on - strand at 3211.275 kb on + strand at 3211.275 kb on + strand at 3211.284 kb on + strand at 3211.285 kb on - strand at 3211.301 kb on + strand at 3211.301 kb on + strand at 3211.302 kb on - strand at 3211.302 kb on - strand at 3211.328 kb on + strand at 3211.328 kb on + strand at 3211.328 kb on + strand at 3211.328 kb on + strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.329 kb on - strand at 3211.330 kb on + strand at 3211.330 kb on + strand at 3211.330 kb on + strand at 3211.331 kb on - strand at 3211.331 kb on - strand at 3211.331 kb on - strand at 3211.331 kb on - strand at 3211.348 kb on + strand at 3211.348 kb on + strand at 3211.348 kb on + strand at 3211.349 kb on - strand at 3211.376 kb on + strand at 3211.377 kb on - strand at 3211.400 kb on + strand at 3211.401 kb on - strand at 3211.401 kb on - strand at 3211.401 kb on - strand at 3211.401 kb on - strand at 3211.401 kb on - strand at 3211.401 kb on - strand at 3211.401 kb on - strand at 3211.424 kb on + strand at 3211.425 kb on - strand at 3211.425 kb on - strand at 3211.425 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 3,208,670 + Pf1N1B4_2859 0.79 +1.6 3,208,670 + Pf1N1B4_2859 0.79 +0.6 3,208,671 - Pf1N1B4_2859 0.79 +0.9 3,208,671 - Pf1N1B4_2859 0.79 -0.3 3,208,671 - Pf1N1B4_2859 0.79 +0.5 3,208,679 + Pf1N1B4_2859 0.79 +0.5 3,208,680 - Pf1N1B4_2859 0.79 +1.0 3,208,680 - Pf1N1B4_2859 0.79 +0.3 3,208,803 + Pf1N1B4_2859 0.83 +1.3 3,209,000 + +0.9 3,209,000 + +1.1 3,209,000 + +1.3 3,209,001 - +0.6 3,209,001 - +1.6 3,209,072 + +0.9 3,209,072 + +1.6 3,209,072 + +1.3 3,209,072 + +0.1 3,209,072 + +0.1 3,209,102 + +2.0 3,209,103 - +4.5 3,209,144 + -0.6 3,209,145 - +1.3 3,209,145 - +0.1 3,209,145 - -1.2 3,209,165 + +1.5 3,209,165 + -0.9 3,209,165 + +0.7 3,209,165 + +2.3 3,209,165 + +1.3 3,209,165 + +2.1 3,209,165 + +1.3 3,209,165 + +0.3 3,209,166 - +2.3 3,209,166 - +2.9 3,209,166 - +1.4 3,209,166 - -0.3 3,209,166 - -0.0 3,209,246 + +0.7 3,209,349 + Pf1N1B4_2860 0.40 -1.9 3,209,349 + Pf1N1B4_2860 0.40 +1.6 3,209,349 + Pf1N1B4_2860 0.40 +0.6 3,209,349 + Pf1N1B4_2860 0.40 +0.5 3,209,349 + Pf1N1B4_2860 0.40 +1.0 3,209,349 + Pf1N1B4_2860 0.40 -0.3 3,209,349 + Pf1N1B4_2860 0.40 +2.3 3,209,349 + Pf1N1B4_2860 0.40 +1.0 3,209,349 + Pf1N1B4_2860 0.40 +1.0 3,209,349 + Pf1N1B4_2860 0.40 +0.3 3,209,349 + Pf1N1B4_2860 0.40 +1.5 3,209,349 + Pf1N1B4_2860 0.40 +0.6 3,209,350 - Pf1N1B4_2860 0.40 -1.5 3,209,350 - Pf1N1B4_2860 0.40 +0.5 3,209,350 - Pf1N1B4_2860 0.40 -1.0 3,209,350 - Pf1N1B4_2860 0.40 -1.3 3,209,350 - Pf1N1B4_2860 0.40 -0.3 3,209,350 - Pf1N1B4_2860 0.40 +2.1 3,209,412 + Pf1N1B4_2860 0.75 +0.3 3,209,412 + Pf1N1B4_2860 0.75 +2.1 3,209,412 + Pf1N1B4_2860 0.75 +2.9 3,209,412 + Pf1N1B4_2860 0.75 +0.6 3,209,412 + Pf1N1B4_2860 0.75 +1.3 3,209,412 + Pf1N1B4_2860 0.75 +0.1 3,209,412 + Pf1N1B4_2860 0.75 +0.3 3,209,412 + Pf1N1B4_2860 0.75 +0.9 3,209,412 + Pf1N1B4_2860 0.75 +0.5 3,209,412 + Pf1N1B4_2860 0.75 +1.0 3,209,412 + Pf1N1B4_2860 0.75 +1.1 3,209,412 + Pf1N1B4_2860 0.75 +1.1 3,209,412 + Pf1N1B4_2860 0.75 -0.3 3,209,412 + Pf1N1B4_2860 0.75 +0.9 3,209,412 + +3.9 3,209,423 + Pf1N1B4_2860 0.81 +0.9 3,209,423 + Pf1N1B4_2860 0.81 +0.5 3,209,445 + +0.2 3,209,445 + +1.3 3,209,445 + +0.7 3,209,445 + +0.9 3,209,445 + +0.7 3,209,445 + +1.8 3,209,445 + +0.4 3,209,445 + -1.1 3,209,445 + +1.8 3,209,445 + +0.3 3,209,445 + +1.0 3,209,445 + +1.2 3,209,445 + +0.7 3,209,445 + +0.7 3,209,445 + +1.9 3,209,445 + +0.2 3,209,446 - -1.2 3,209,446 - -1.5 3,209,451 + +1.2 3,209,451 + +0.5 3,209,451 + +1.9 3,209,451 + +2.6 3,209,451 + +2.5 3,209,451 + +1.1 3,209,451 + +0.9 3,209,451 + -0.7 3,209,451 + +0.8 3,209,451 + +0.1 3,209,451 + +1.7 3,209,451 + +1.3 3,209,451 + +0.4 3,209,451 + +1.7 3,209,451 + +1.6 3,209,451 + +1.5 3,209,451 + +0.1 3,209,451 + +1.8 3,209,451 + +1.1 3,209,451 + +1.5 3,209,451 + -0.3 3,209,451 + +0.8 3,209,451 + +1.6 3,209,451 + -0.5 3,209,451 + -2.1 3,209,451 + +0.9 3,209,451 + +0.7 3,209,451 + +1.6 3,209,451 + +0.3 3,209,451 + +1.8 3,209,451 + +0.3 3,209,451 + +2.5 3,209,451 + -1.0 3,209,451 + +0.2 3,209,451 + +1.5 3,209,451 + +0.7 3,209,451 + +0.5 3,209,451 + -1.5 3,209,451 + +0.3 3,209,451 + -1.7 3,209,452 - -0.3 3,209,452 - +1.9 3,209,452 - +0.6 3,209,452 - +0.7 3,209,452 - +0.5 3,209,452 - -0.3 3,209,452 - -0.5 3,209,452 - -1.3 3,209,452 - -0.3 3,209,452 - -1.7 3,209,452 - +1.1 3,209,452 - -0.0 3,209,472 + -0.3 3,209,680 + Pf1N1B4_2861 0.16 -1.0 3,209,813 + Pf1N1B4_2861 0.31 -0.1 3,209,813 + Pf1N1B4_2861 0.31 +0.7 3,209,907 - Pf1N1B4_2861 0.42 -2.6 3,210,002 + Pf1N1B4_2861 0.52 -1.0 3,210,002 + Pf1N1B4_2861 0.52 -2.6 3,210,002 + Pf1N1B4_2861 0.52 -1.3 3,210,077 + Pf1N1B4_2861 0.61 -0.7 3,210,077 + Pf1N1B4_2861 0.61 -1.5 3,210,077 + Pf1N1B4_2861 0.61 -2.0 3,210,077 + Pf1N1B4_2861 0.61 -0.0 3,210,077 + +1.3 3,210,167 + Pf1N1B4_2861 0.71 -1.7 3,210,167 + Pf1N1B4_2861 0.71 +1.1 3,210,168 - Pf1N1B4_2861 0.71 -1.3 3,210,168 - Pf1N1B4_2861 0.71 -1.0 3,210,367 + -1.3 3,210,368 - +0.3 3,210,368 - +1.0 3,210,368 - -1.3 3,210,385 - -0.6 3,210,404 + +0.1 3,210,429 + -0.0 3,210,429 + +1.3 3,210,430 - +0.9 3,210,441 + +0.2 3,210,441 + -1.5 3,210,441 + +0.3 3,210,441 + -1.5 3,210,441 + -0.7 3,210,441 + +0.6 3,210,441 + -1.0 3,210,441 + -0.7 3,210,441 + -1.0 3,210,441 + -1.0 3,210,441 + +0.8 3,210,441 + -0.7 3,210,442 - -0.7 3,210,442 - +0.1 3,210,442 - +0.1 3,210,442 - -0.7 3,210,513 + +1.9 3,210,638 + +1.7 3,210,639 - -1.0 3,210,639 - -1.7 3,210,639 - +1.7 3,210,639 - +2.3 3,210,639 - -0.0 3,211,102 - -0.7 3,211,102 - -1.3 3,211,102 - -0.1 3,211,275 + -1.3 3,211,275 + -1.0 3,211,284 + +0.3 3,211,285 - +0.1 3,211,301 + -0.5 3,211,301 + -0.6 3,211,302 - -0.9 3,211,302 - +0.1 3,211,328 + -0.0 3,211,328 + +1.3 3,211,328 + -0.7 3,211,328 + -1.5 3,211,329 - +1.3 3,211,329 - -1.3 3,211,329 - -1.3 3,211,329 - -0.7 3,211,329 - -0.3 3,211,329 - +0.7 3,211,329 - -0.4 3,211,329 - -1.3 3,211,329 - +0.3 3,211,329 - +0.3 3,211,329 - +1.3 3,211,330 + -0.0 3,211,330 + +0.2 3,211,330 + -0.3 3,211,331 - -0.5 3,211,331 - -0.7 3,211,331 - +0.1 3,211,331 - -2.3 3,211,348 + -0.4 3,211,348 + -0.3 3,211,348 + -0.9 3,211,349 - -0.0 3,211,376 + +0.6 3,211,377 - +0.3 3,211,400 + -0.0 3,211,401 - -0.7 3,211,401 - +0.7 3,211,401 - -0.3 3,211,401 - +0.3 3,211,401 - +1.3 3,211,401 - -0.3 3,211,401 - -0.2 3,211,424 + -0.1 3,211,425 - +0.6 3,211,425 - -1.0 3,211,425 - -1.8
Or see this region's nucleotide sequence