Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2731 and Pf1N1B4_2732 are separated by 142 nucleotides Pf1N1B4_2732 and Pf1N1B4_2733 are separated by 22 nucleotides Pf1N1B4_2733 and Pf1N1B4_2734 are separated by 119 nucleotides
Pf1N1B4_2731 - Uncharacterized protein conserved in bacteria, at 3,070,529 to 3,071,281
_2731
Pf1N1B4_2732 - FIG00954572: hypothetical protein, at 3,071,424 to 3,071,903
_2732
Pf1N1B4_2733 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 3,071,926 to 3,072,537
_2733
Pf1N1B4_2734 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 3,072,657 to 3,073,112
_2734
Position (kb)
3071
3072 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3070.452 kb on - strand at 3070.503 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.518 kb on - strand at 3070.518 kb on - strand at 3070.665 kb on - strand, within Pf1N1B4_2731 at 3070.665 kb on - strand, within Pf1N1B4_2731 at 3070.973 kb on + strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3070.974 kb on - strand, within Pf1N1B4_2731 at 3071.072 kb on - strand, within Pf1N1B4_2731 at 3071.191 kb on + strand, within Pf1N1B4_2731 at 3071.192 kb on - strand, within Pf1N1B4_2731 at 3071.192 kb on - strand, within Pf1N1B4_2731 at 3071.192 kb on - strand, within Pf1N1B4_2731 at 3071.228 kb on + strand at 3071.229 kb on - strand at 3071.229 kb on - strand at 3071.229 kb on - strand at 3071.230 kb on + strand at 3071.355 kb on + strand at 3071.355 kb on + strand at 3071.355 kb on + strand at 3071.356 kb on - strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.420 kb on + strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.421 kb on - strand at 3071.463 kb on + strand at 3071.463 kb on + strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.464 kb on - strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.466 kb on + strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.467 kb on - strand at 3071.485 kb on - strand, within Pf1N1B4_2732 at 3071.502 kb on + strand, within Pf1N1B4_2732 at 3071.502 kb on + strand, within Pf1N1B4_2732 at 3071.503 kb on - strand, within Pf1N1B4_2732 at 3071.528 kb on + strand, within Pf1N1B4_2732 at 3071.528 kb on + strand, within Pf1N1B4_2732 at 3071.529 kb on - strand, within Pf1N1B4_2732 at 3071.529 kb on - strand, within Pf1N1B4_2732 at 3071.553 kb on + strand, within Pf1N1B4_2732 at 3071.553 kb on + strand, within Pf1N1B4_2732 at 3071.553 kb on + strand at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.554 kb on - strand, within Pf1N1B4_2732 at 3071.643 kb on + strand, within Pf1N1B4_2732 at 3071.643 kb on + strand, within Pf1N1B4_2732 at 3071.643 kb on + strand, within Pf1N1B4_2732 at 3071.643 kb on + strand, within Pf1N1B4_2732 at 3071.644 kb on - strand, within Pf1N1B4_2732 at 3071.644 kb on - strand, within Pf1N1B4_2732 at 3071.644 kb on - strand, within Pf1N1B4_2732 at 3071.644 kb on - strand, within Pf1N1B4_2732 at 3071.790 kb on - strand, within Pf1N1B4_2732 at 3071.936 kb on + strand at 3071.936 kb on + strand at 3071.936 kb on + strand at 3071.937 kb on - strand at 3072.081 kb on + strand, within Pf1N1B4_2733 at 3072.082 kb on - strand at 3072.082 kb on - strand, within Pf1N1B4_2733 at 3072.082 kb on - strand, within Pf1N1B4_2733 at 3072.223 kb on + strand, within Pf1N1B4_2733 at 3072.224 kb on - strand, within Pf1N1B4_2733 at 3072.546 kb on + strand at 3072.546 kb on + strand at 3072.547 kb on - strand at 3072.665 kb on + strand at 3072.665 kb on + strand at 3072.666 kb on - strand at 3072.666 kb on - strand at 3072.875 kb on - strand, within Pf1N1B4_2734
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 3,070,452 - -1.2 3,070,503 + +1.4 3,070,517 + -0.3 3,070,517 + +0.1 3,070,517 + +0.3 3,070,517 + +0.4 3,070,517 + -0.9 3,070,518 - -0.4 3,070,518 - -0.4 3,070,665 - Pf1N1B4_2731 0.18 +1.4 3,070,665 - Pf1N1B4_2731 0.18 -0.9 3,070,973 + Pf1N1B4_2731 0.59 -2.3 3,070,974 - Pf1N1B4_2731 0.59 -0.9 3,070,974 - Pf1N1B4_2731 0.59 +0.4 3,070,974 - Pf1N1B4_2731 0.59 -1.3 3,070,974 - Pf1N1B4_2731 0.59 -1.4 3,071,072 - Pf1N1B4_2731 0.72 -1.4 3,071,191 + Pf1N1B4_2731 0.88 -1.2 3,071,192 - Pf1N1B4_2731 0.88 +0.4 3,071,192 - Pf1N1B4_2731 0.88 +0.8 3,071,192 - Pf1N1B4_2731 0.88 +1.1 3,071,228 + +0.1 3,071,229 - -1.0 3,071,229 - +2.2 3,071,229 - +1.6 3,071,230 + +1.7 3,071,355 + +0.8 3,071,355 + -0.9 3,071,355 + -0.8 3,071,356 - +0.9 3,071,420 + +2.0 3,071,420 + -1.1 3,071,420 + +0.4 3,071,420 + +1.2 3,071,420 + +0.3 3,071,420 + +0.8 3,071,420 + -0.0 3,071,420 + -0.2 3,071,420 + -0.4 3,071,421 - +0.1 3,071,421 - +0.4 3,071,421 - +0.3 3,071,421 - -1.4 3,071,421 - -0.0 3,071,421 - +0.8 3,071,421 - +0.8 3,071,421 - +2.4 3,071,463 + -0.6 3,071,463 + +2.1 3,071,464 - +0.9 3,071,464 - +2.0 3,071,464 - +0.3 3,071,464 - -1.5 3,071,464 - -2.1 3,071,464 - +1.0 3,071,466 + +0.4 3,071,466 + +0.8 3,071,466 + +0.3 3,071,466 + +0.8 3,071,466 + -0.2 3,071,466 + +1.4 3,071,466 + +0.2 3,071,467 - -1.6 3,071,467 - -1.8 3,071,467 - +1.0 3,071,467 - +1.1 3,071,467 - -0.6 3,071,467 - -0.2 3,071,467 - +0.2 3,071,467 - -0.3 3,071,485 - Pf1N1B4_2732 0.13 +0.9 3,071,502 + Pf1N1B4_2732 0.16 +0.8 3,071,502 + Pf1N1B4_2732 0.16 +0.4 3,071,503 - Pf1N1B4_2732 0.16 +1.1 3,071,528 + Pf1N1B4_2732 0.22 -0.6 3,071,528 + Pf1N1B4_2732 0.22 +2.1 3,071,529 - Pf1N1B4_2732 0.22 +0.6 3,071,529 - Pf1N1B4_2732 0.22 +1.4 3,071,553 + Pf1N1B4_2732 0.27 -2.1 3,071,553 + Pf1N1B4_2732 0.27 -0.4 3,071,553 + +1.1 3,071,554 - Pf1N1B4_2732 0.27 +1.4 3,071,554 - +1.4 3,071,554 - Pf1N1B4_2732 0.27 -0.2 3,071,554 - Pf1N1B4_2732 0.27 +1.4 3,071,554 - Pf1N1B4_2732 0.27 +0.1 3,071,554 - Pf1N1B4_2732 0.27 -0.0 3,071,554 - +0.8 3,071,554 - Pf1N1B4_2732 0.27 -1.8 3,071,554 - Pf1N1B4_2732 0.27 -1.4 3,071,554 - Pf1N1B4_2732 0.27 +0.7 3,071,643 + Pf1N1B4_2732 0.46 -2.1 3,071,643 + Pf1N1B4_2732 0.46 +0.2 3,071,643 + Pf1N1B4_2732 0.46 +0.9 3,071,643 + Pf1N1B4_2732 0.46 +0.4 3,071,644 - Pf1N1B4_2732 0.46 +0.6 3,071,644 - Pf1N1B4_2732 0.46 +1.7 3,071,644 - Pf1N1B4_2732 0.46 -2.1 3,071,644 - Pf1N1B4_2732 0.46 -0.3 3,071,790 - Pf1N1B4_2732 0.76 +0.3 3,071,936 + +2.2 3,071,936 + +0.8 3,071,936 + +0.1 3,071,937 - +2.1 3,072,081 + Pf1N1B4_2733 0.25 +0.4 3,072,082 - -0.2 3,072,082 - Pf1N1B4_2733 0.25 -0.6 3,072,082 - Pf1N1B4_2733 0.25 +0.5 3,072,223 + Pf1N1B4_2733 0.49 +1.4 3,072,224 - Pf1N1B4_2733 0.49 -1.6 3,072,546 + +1.0 3,072,546 + -0.3 3,072,547 - +1.7 3,072,665 + +1.4 3,072,665 + -1.2 3,072,666 - -0.2 3,072,666 - -0.7 3,072,875 - Pf1N1B4_2734 0.48 +0.7
Or see this region's nucleotide sequence