Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2728 and Pf1N1B4_2729 are separated by 141 nucleotides Pf1N1B4_2729 and Pf1N1B4_2730 are separated by 88 nucleotides Pf1N1B4_2730 and Pf1N1B4_2731 are separated by 178 nucleotides
Pf1N1B4_2728 - ATP-dependent helicase HrpB, at 3,065,847 to 3,068,363
_2728
Pf1N1B4_2729 - endonuclease/exonuclease/phosphatase family protein, at 3,068,505 to 3,069,587
_2729
Pf1N1B4_2730 - FIG00958173: hypothetical protein, at 3,069,676 to 3,070,350
_2730
Pf1N1B4_2731 - Uncharacterized protein conserved in bacteria, at 3,070,529 to 3,071,281
_2731
Position (kb)
3068
3069
3070 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3067.569 kb on + strand, within Pf1N1B4_2728 at 3067.570 kb on - strand, within Pf1N1B4_2728 at 3067.865 kb on + strand, within Pf1N1B4_2728 at 3067.961 kb on + strand, within Pf1N1B4_2728 at 3067.961 kb on + strand, within Pf1N1B4_2728 at 3068.000 kb on + strand, within Pf1N1B4_2728 at 3068.000 kb on + strand, within Pf1N1B4_2728 at 3068.000 kb on + strand, within Pf1N1B4_2728 at 3068.000 kb on + strand, within Pf1N1B4_2728 at 3068.000 kb on + strand, within Pf1N1B4_2728 at 3068.000 kb on + strand, within Pf1N1B4_2728 at 3068.091 kb on + strand, within Pf1N1B4_2728 at 3068.259 kb on + strand at 3068.259 kb on + strand at 3068.260 kb on - strand at 3068.260 kb on - strand at 3068.304 kb on + strand at 3068.361 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.433 kb on + strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.434 kb on - strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.567 kb on + strand at 3068.568 kb on - strand at 3068.568 kb on - strand at 3068.615 kb on - strand, within Pf1N1B4_2729 at 3068.624 kb on - strand, within Pf1N1B4_2729 at 3068.624 kb on - strand, within Pf1N1B4_2729 at 3068.624 kb on - strand, within Pf1N1B4_2729 at 3068.732 kb on - strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.747 kb on + strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.748 kb on - strand, within Pf1N1B4_2729 at 3068.770 kb on + strand, within Pf1N1B4_2729 at 3068.771 kb on - strand, within Pf1N1B4_2729 at 3068.803 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.866 kb on + strand, within Pf1N1B4_2729 at 3068.867 kb on - strand, within Pf1N1B4_2729 at 3068.867 kb on - strand at 3068.867 kb on - strand, within Pf1N1B4_2729 at 3068.867 kb on - strand, within Pf1N1B4_2729 at 3068.867 kb on - strand, within Pf1N1B4_2729 at 3069.054 kb on + strand, within Pf1N1B4_2729 at 3069.124 kb on + strand, within Pf1N1B4_2729 at 3069.124 kb on + strand, within Pf1N1B4_2729 at 3069.229 kb on + strand, within Pf1N1B4_2729 at 3069.386 kb on - strand, within Pf1N1B4_2729 at 3069.403 kb on + strand, within Pf1N1B4_2729 at 3069.403 kb on + strand, within Pf1N1B4_2729 at 3069.517 kb on + strand at 3069.517 kb on + strand at 3069.597 kb on + strand at 3069.598 kb on - strand at 3069.598 kb on - strand at 3069.599 kb on + strand at 3069.599 kb on + strand at 3069.599 kb on + strand at 3069.600 kb on - strand at 3069.600 kb on - strand at 3069.600 kb on - strand at 3069.605 kb on + strand at 3069.656 kb on + strand at 3069.816 kb on - strand, within Pf1N1B4_2730 at 3069.962 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.010 kb on + strand, within Pf1N1B4_2730 at 3070.011 kb on - strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.163 kb on + strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.164 kb on - strand, within Pf1N1B4_2730 at 3070.165 kb on + strand, within Pf1N1B4_2730 at 3070.165 kb on + strand, within Pf1N1B4_2730 at 3070.192 kb on + strand, within Pf1N1B4_2730 at 3070.192 kb on + strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.193 kb on - strand, within Pf1N1B4_2730 at 3070.316 kb on + strand at 3070.316 kb on + strand at 3070.316 kb on + strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.317 kb on - strand at 3070.333 kb on + strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.334 kb on - strand at 3070.388 kb on + strand at 3070.418 kb on + strand at 3070.418 kb on + strand at 3070.418 kb on + strand at 3070.418 kb on + strand at 3070.419 kb on - strand at 3070.419 kb on - strand at 3070.419 kb on - strand at 3070.452 kb on - strand at 3070.503 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.517 kb on + strand at 3070.518 kb on - strand at 3070.518 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 3,067,569 + Pf1N1B4_2728 0.68 -2.4 3,067,570 - Pf1N1B4_2728 0.68 -0.2 3,067,865 + Pf1N1B4_2728 0.80 -1.8 3,067,961 + Pf1N1B4_2728 0.84 -1.4 3,067,961 + Pf1N1B4_2728 0.84 -0.9 3,068,000 + Pf1N1B4_2728 0.86 -1.6 3,068,000 + Pf1N1B4_2728 0.86 +0.6 3,068,000 + Pf1N1B4_2728 0.86 +1.2 3,068,000 + Pf1N1B4_2728 0.86 -1.4 3,068,000 + Pf1N1B4_2728 0.86 +0.8 3,068,000 + Pf1N1B4_2728 0.86 -0.2 3,068,091 + Pf1N1B4_2728 0.89 -0.7 3,068,259 + +2.4 3,068,259 + +0.6 3,068,260 - -0.2 3,068,260 - -0.6 3,068,304 + +0.3 3,068,361 + -0.2 3,068,433 + +0.4 3,068,433 + -0.4 3,068,433 + -0.7 3,068,433 + -0.0 3,068,433 + +1.4 3,068,433 + -0.2 3,068,433 + -0.2 3,068,433 + -0.9 3,068,433 + +1.4 3,068,433 + +0.5 3,068,433 + -0.2 3,068,433 + +1.8 3,068,433 + +0.4 3,068,433 + -1.1 3,068,433 + -0.8 3,068,433 + -1.2 3,068,433 + +1.2 3,068,433 + -1.1 3,068,433 + -0.9 3,068,433 + +0.4 3,068,433 + +0.4 3,068,433 + -0.8 3,068,434 - -1.2 3,068,434 - -0.5 3,068,434 - +1.4 3,068,434 - +0.3 3,068,434 - +4.0 3,068,434 - +0.0 3,068,434 - +0.5 3,068,434 - +2.4 3,068,434 - +1.8 3,068,434 - +1.4 3,068,434 - +0.4 3,068,434 - -1.0 3,068,434 - +0.6 3,068,434 - +1.0 3,068,434 - +0.6 3,068,434 - -0.6 3,068,434 - +0.8 3,068,434 - -0.6 3,068,434 - +0.4 3,068,434 - +1.1 3,068,434 - +0.4 3,068,434 - +0.5 3,068,434 - +2.0 3,068,434 - -0.8 3,068,567 + -0.4 3,068,567 + +0.6 3,068,567 + -1.5 3,068,567 + -0.9 3,068,567 + -1.2 3,068,567 + +0.2 3,068,567 + -1.8 3,068,567 + -0.5 3,068,567 + +1.0 3,068,567 + +0.2 3,068,567 + +0.9 3,068,568 - +0.2 3,068,568 - +0.4 3,068,615 - Pf1N1B4_2729 0.10 -1.2 3,068,624 - Pf1N1B4_2729 0.11 +1.4 3,068,624 - Pf1N1B4_2729 0.11 -0.4 3,068,624 - Pf1N1B4_2729 0.11 +0.7 3,068,732 - Pf1N1B4_2729 0.21 -1.2 3,068,747 + Pf1N1B4_2729 0.22 +0.1 3,068,747 + Pf1N1B4_2729 0.22 +2.4 3,068,747 + Pf1N1B4_2729 0.22 +0.6 3,068,747 + Pf1N1B4_2729 0.22 -0.3 3,068,747 + Pf1N1B4_2729 0.22 +0.4 3,068,747 + Pf1N1B4_2729 0.22 +1.6 3,068,748 - Pf1N1B4_2729 0.22 -0.9 3,068,748 - Pf1N1B4_2729 0.22 +0.2 3,068,748 - Pf1N1B4_2729 0.22 +1.0 3,068,748 - Pf1N1B4_2729 0.22 +1.1 3,068,748 - Pf1N1B4_2729 0.22 +0.4 3,068,748 - Pf1N1B4_2729 0.22 +1.4 3,068,748 - -0.2 3,068,748 - Pf1N1B4_2729 0.22 +0.7 3,068,748 - Pf1N1B4_2729 0.22 +0.8 3,068,748 - Pf1N1B4_2729 0.22 -0.4 3,068,770 + Pf1N1B4_2729 0.24 +0.4 3,068,771 - Pf1N1B4_2729 0.25 +1.4 3,068,803 + Pf1N1B4_2729 0.28 +1.8 3,068,866 + Pf1N1B4_2729 0.33 +0.1 3,068,866 + Pf1N1B4_2729 0.33 -1.9 3,068,866 + Pf1N1B4_2729 0.33 -1.4 3,068,866 + Pf1N1B4_2729 0.33 +1.4 3,068,866 + Pf1N1B4_2729 0.33 +1.0 3,068,866 + Pf1N1B4_2729 0.33 -0.6 3,068,867 - Pf1N1B4_2729 0.33 -1.2 3,068,867 - +1.4 3,068,867 - Pf1N1B4_2729 0.33 +0.4 3,068,867 - Pf1N1B4_2729 0.33 -1.4 3,068,867 - Pf1N1B4_2729 0.33 +0.2 3,069,054 + Pf1N1B4_2729 0.51 +1.2 3,069,124 + Pf1N1B4_2729 0.57 +0.1 3,069,124 + Pf1N1B4_2729 0.57 +1.4 3,069,229 + Pf1N1B4_2729 0.67 +0.4 3,069,386 - Pf1N1B4_2729 0.81 +0.4 3,069,403 + Pf1N1B4_2729 0.83 -1.4 3,069,403 + Pf1N1B4_2729 0.83 +1.4 3,069,517 + -0.2 3,069,517 + -0.2 3,069,597 + -0.5 3,069,598 - -0.2 3,069,598 - +0.7 3,069,599 + -0.9 3,069,599 + +1.8 3,069,599 + +3.4 3,069,600 - +0.6 3,069,600 - +2.5 3,069,600 - +0.2 3,069,605 + -0.2 3,069,656 + -0.2 3,069,816 - Pf1N1B4_2730 0.21 -0.8 3,069,962 + Pf1N1B4_2730 0.42 +0.4 3,070,010 + +3.4 3,070,010 + Pf1N1B4_2730 0.49 -0.2 3,070,010 + Pf1N1B4_2730 0.49 -0.5 3,070,010 + Pf1N1B4_2730 0.49 -0.4 3,070,010 + Pf1N1B4_2730 0.49 -0.2 3,070,011 - Pf1N1B4_2730 0.50 -1.9 3,070,163 + Pf1N1B4_2730 0.72 +0.2 3,070,163 + Pf1N1B4_2730 0.72 +0.6 3,070,163 + Pf1N1B4_2730 0.72 -0.7 3,070,163 + Pf1N1B4_2730 0.72 +0.4 3,070,163 + Pf1N1B4_2730 0.72 +1.0 3,070,163 + Pf1N1B4_2730 0.72 +0.8 3,070,164 - Pf1N1B4_2730 0.72 +0.1 3,070,164 - Pf1N1B4_2730 0.72 +0.2 3,070,164 - Pf1N1B4_2730 0.72 +0.1 3,070,164 - Pf1N1B4_2730 0.72 +1.0 3,070,164 - Pf1N1B4_2730 0.72 +1.4 3,070,164 - Pf1N1B4_2730 0.72 +1.0 3,070,165 + Pf1N1B4_2730 0.72 +1.1 3,070,165 + Pf1N1B4_2730 0.72 -0.0 3,070,192 + Pf1N1B4_2730 0.76 +0.8 3,070,192 + Pf1N1B4_2730 0.76 -0.7 3,070,193 - Pf1N1B4_2730 0.77 -1.2 3,070,193 - Pf1N1B4_2730 0.77 +1.7 3,070,193 - Pf1N1B4_2730 0.77 -0.8 3,070,193 - Pf1N1B4_2730 0.77 -1.4 3,070,316 + +1.4 3,070,316 + +0.2 3,070,316 + +0.2 3,070,317 - +2.1 3,070,317 - +0.1 3,070,317 - -0.2 3,070,317 - +1.4 3,070,317 - -0.9 3,070,317 - -0.1 3,070,333 + +0.9 3,070,334 - +0.4 3,070,334 - -0.4 3,070,334 - -1.9 3,070,334 - -2.1 3,070,334 - -1.4 3,070,334 - +0.7 3,070,334 - +0.1 3,070,334 - -0.3 3,070,334 - +0.6 3,070,388 + +1.4 3,070,418 + +0.8 3,070,418 + -0.5 3,070,418 + +0.6 3,070,418 + -0.1 3,070,419 - -0.7 3,070,419 - -1.6 3,070,419 - +0.2 3,070,452 - -1.2 3,070,503 + +1.4 3,070,517 + -0.3 3,070,517 + +0.1 3,070,517 + +0.3 3,070,517 + +0.4 3,070,517 + -0.9 3,070,518 - -0.4 3,070,518 - -0.4
Or see this region's nucleotide sequence