Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2455 and Pf1N1B4_2456 are separated by 14 nucleotides Pf1N1B4_2456 and Pf1N1B4_2457 overlap by 4 nucleotides
Pf1N1B4_2455 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 2,784,943 to 2,786,193
_2455
Pf1N1B4_2456 - Sarcosine oxidase delta subunit (EC 1.5.3.1), at 2,786,208 to 2,786,537
_2456
Pf1N1B4_2457 - Sarcosine oxidase alpha subunit (EC 1.5.3.1), at 2,786,534 to 2,789,551
_2457
Position (kb)
2786
2787 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2785.267 kb on + strand, within Pf1N1B4_2455 at 2785.267 kb on + strand, within Pf1N1B4_2455 at 2785.268 kb on - strand, within Pf1N1B4_2455 at 2785.268 kb on - strand, within Pf1N1B4_2455 at 2785.268 kb on - strand, within Pf1N1B4_2455 at 2785.789 kb on + strand, within Pf1N1B4_2455 at 2785.789 kb on + strand, within Pf1N1B4_2455 at 2785.790 kb on - strand, within Pf1N1B4_2455 at 2786.192 kb on - strand at 2786.207 kb on + strand at 2786.207 kb on + strand at 2786.208 kb on - strand at 2786.208 kb on - strand at 2786.217 kb on - strand at 2786.334 kb on + strand, within Pf1N1B4_2456 at 2786.334 kb on + strand, within Pf1N1B4_2456 at 2786.334 kb on + strand, within Pf1N1B4_2456 at 2786.335 kb on - strand, within Pf1N1B4_2456 at 2786.335 kb on - strand, within Pf1N1B4_2456 at 2786.406 kb on + strand, within Pf1N1B4_2456 at 2786.407 kb on - strand, within Pf1N1B4_2456 at 2786.436 kb on + strand, within Pf1N1B4_2456 at 2786.454 kb on + strand, within Pf1N1B4_2456 at 2786.454 kb on + strand, within Pf1N1B4_2456 at 2786.454 kb on + strand, within Pf1N1B4_2456 at 2786.533 kb on + strand at 2786.533 kb on + strand at 2786.534 kb on - strand at 2786.534 kb on - strand at 2786.534 kb on - strand at 2786.615 kb on + strand at 2786.615 kb on + strand at 2786.616 kb on - strand at 2786.693 kb on + strand at 2786.693 kb on + strand at 2786.694 kb on - strand at 2786.694 kb on - strand at 2786.788 kb on - strand at 2786.810 kb on + strand at 2786.811 kb on - strand at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.916 kb on - strand, within Pf1N1B4_2457 at 2786.918 kb on + strand, within Pf1N1B4_2457 at 2786.933 kb on + strand at 2786.957 kb on + strand, within Pf1N1B4_2457 at 2786.957 kb on + strand, within Pf1N1B4_2457 at 2786.957 kb on + strand, within Pf1N1B4_2457 at 2786.966 kb on + strand, within Pf1N1B4_2457 at 2786.966 kb on + strand, within Pf1N1B4_2457 at 2786.966 kb on + strand, within Pf1N1B4_2457 at 2786.966 kb on + strand, within Pf1N1B4_2457 at 2786.967 kb on - strand, within Pf1N1B4_2457 at 2786.967 kb on - strand, within Pf1N1B4_2457 at 2786.967 kb on - strand, within Pf1N1B4_2457 at 2787.023 kb on + strand, within Pf1N1B4_2457 at 2787.024 kb on - strand, within Pf1N1B4_2457 at 2787.029 kb on + strand, within Pf1N1B4_2457 at 2787.030 kb on - strand at 2787.030 kb on - strand, within Pf1N1B4_2457 at 2787.289 kb on + strand, within Pf1N1B4_2457 at 2787.289 kb on + strand, within Pf1N1B4_2457 at 2787.289 kb on + strand, within Pf1N1B4_2457 at 2787.290 kb on - strand, within Pf1N1B4_2457 at 2787.290 kb on - strand, within Pf1N1B4_2457 at 2787.290 kb on - strand, within Pf1N1B4_2457 at 2787.390 kb on + strand, within Pf1N1B4_2457 at 2787.390 kb on + strand, within Pf1N1B4_2457 at 2787.391 kb on - strand, within Pf1N1B4_2457 at 2787.428 kb on + strand, within Pf1N1B4_2457 at 2787.428 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.479 kb on + strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.480 kb on - strand, within Pf1N1B4_2457 at 2787.498 kb on + strand, within Pf1N1B4_2457
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 2,785,267 + Pf1N1B4_2455 0.26 -0.1 2,785,267 + Pf1N1B4_2455 0.26 -0.2 2,785,268 - Pf1N1B4_2455 0.26 +1.2 2,785,268 - Pf1N1B4_2455 0.26 +0.8 2,785,268 - Pf1N1B4_2455 0.26 +0.2 2,785,789 + Pf1N1B4_2455 0.68 -1.9 2,785,789 + Pf1N1B4_2455 0.68 -0.9 2,785,790 - Pf1N1B4_2455 0.68 +1.0 2,786,192 - +0.7 2,786,207 + -1.0 2,786,207 + -0.6 2,786,208 - -0.2 2,786,208 - -0.3 2,786,217 - +0.9 2,786,334 + Pf1N1B4_2456 0.38 -0.9 2,786,334 + Pf1N1B4_2456 0.38 -1.9 2,786,334 + Pf1N1B4_2456 0.38 -1.6 2,786,335 - Pf1N1B4_2456 0.38 -1.6 2,786,335 - Pf1N1B4_2456 0.38 -1.5 2,786,406 + Pf1N1B4_2456 0.60 -0.8 2,786,407 - Pf1N1B4_2456 0.60 -0.6 2,786,436 + Pf1N1B4_2456 0.69 +1.2 2,786,454 + Pf1N1B4_2456 0.75 -0.9 2,786,454 + Pf1N1B4_2456 0.75 +1.8 2,786,454 + Pf1N1B4_2456 0.75 +1.4 2,786,533 + -0.2 2,786,533 + +0.1 2,786,534 - +1.4 2,786,534 - -0.3 2,786,534 - -0.4 2,786,615 + +0.1 2,786,615 + +1.6 2,786,616 - -0.7 2,786,693 + +0.3 2,786,693 + +0.4 2,786,694 - +0.0 2,786,694 - +0.1 2,786,788 - +0.9 2,786,810 + +0.0 2,786,811 - -0.1 2,786,916 - Pf1N1B4_2457 0.13 +0.9 2,786,916 - Pf1N1B4_2457 0.13 -1.6 2,786,916 - Pf1N1B4_2457 0.13 -0.2 2,786,916 - Pf1N1B4_2457 0.13 -0.3 2,786,916 - Pf1N1B4_2457 0.13 -1.0 2,786,916 - Pf1N1B4_2457 0.13 +0.4 2,786,918 + Pf1N1B4_2457 0.13 -0.4 2,786,933 + -0.2 2,786,957 + Pf1N1B4_2457 0.14 +2.1 2,786,957 + Pf1N1B4_2457 0.14 +1.2 2,786,957 + Pf1N1B4_2457 0.14 +0.2 2,786,966 + Pf1N1B4_2457 0.14 -0.2 2,786,966 + Pf1N1B4_2457 0.14 +1.4 2,786,966 + Pf1N1B4_2457 0.14 -0.2 2,786,966 + Pf1N1B4_2457 0.14 -1.7 2,786,967 - Pf1N1B4_2457 0.14 -0.9 2,786,967 - Pf1N1B4_2457 0.14 +1.2 2,786,967 - Pf1N1B4_2457 0.14 +2.2 2,787,023 + Pf1N1B4_2457 0.16 +0.3 2,787,024 - Pf1N1B4_2457 0.16 -0.5 2,787,029 + Pf1N1B4_2457 0.16 +0.4 2,787,030 - +2.4 2,787,030 - Pf1N1B4_2457 0.16 -0.8 2,787,289 + Pf1N1B4_2457 0.25 -1.2 2,787,289 + Pf1N1B4_2457 0.25 +1.3 2,787,289 + Pf1N1B4_2457 0.25 -0.4 2,787,290 - Pf1N1B4_2457 0.25 +1.8 2,787,290 - Pf1N1B4_2457 0.25 -0.4 2,787,290 - Pf1N1B4_2457 0.25 -0.2 2,787,390 + Pf1N1B4_2457 0.28 +0.7 2,787,390 + Pf1N1B4_2457 0.28 -0.0 2,787,391 - Pf1N1B4_2457 0.28 -0.3 2,787,428 + Pf1N1B4_2457 0.30 -0.2 2,787,428 + Pf1N1B4_2457 0.30 +0.2 2,787,479 + Pf1N1B4_2457 0.31 +0.3 2,787,479 + Pf1N1B4_2457 0.31 -0.2 2,787,479 + Pf1N1B4_2457 0.31 +0.4 2,787,479 + Pf1N1B4_2457 0.31 +2.4 2,787,479 + Pf1N1B4_2457 0.31 -0.8 2,787,479 + Pf1N1B4_2457 0.31 +0.1 2,787,479 + Pf1N1B4_2457 0.31 +0.6 2,787,479 + Pf1N1B4_2457 0.31 -0.6 2,787,480 - Pf1N1B4_2457 0.31 +0.0 2,787,480 - Pf1N1B4_2457 0.31 +1.4 2,787,480 - Pf1N1B4_2457 0.31 -3.6 2,787,480 - Pf1N1B4_2457 0.31 -0.2 2,787,480 - Pf1N1B4_2457 0.31 -2.0 2,787,498 + Pf1N1B4_2457 0.32 +1.0
Or see this region's nucleotide sequence