Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2268 and Pf1N1B4_2269 are separated by 2 nucleotides Pf1N1B4_2269 and Pf1N1B4_2270 are separated by 129 nucleotides Pf1N1B4_2270 and Pf1N1B4_2271 are separated by 135 nucleotides Pf1N1B4_2271 and Pf1N1B4_2272 are separated by 63 nucleotides
Pf1N1B4_2268 - oxidoreductase, 2OG-Fe(II) oxygenase family, at 2,585,781 to 2,586,413
_2268
Pf1N1B4_2269 - Purine nucleoside phosphorylase (EC 2.4.2.1), at 2,586,416 to 2,586,901
_2269
Pf1N1B4_2270 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 2,587,031 to 2,587,618
_2270
Pf1N1B4_2271 - ATPases of the AAA+ class, at 2,587,754 to 2,588,179
_2271
Pf1N1B4_2272 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 2,588,243 to 2,589,472
_2272
Position (kb)
2587
2588 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2586.045 kb on + strand, within Pf1N1B4_2268 at 2586.045 kb on + strand, within Pf1N1B4_2268 at 2586.045 kb on + strand, within Pf1N1B4_2268 at 2586.045 kb on + strand, within Pf1N1B4_2268 at 2586.045 kb on + strand, within Pf1N1B4_2268 at 2586.045 kb on + strand, within Pf1N1B4_2268 at 2586.045 kb on + strand, within Pf1N1B4_2268 at 2586.045 kb on + strand, within Pf1N1B4_2268 at 2586.046 kb on - strand, within Pf1N1B4_2268 at 2586.046 kb on - strand, within Pf1N1B4_2268 at 2586.046 kb on - strand, within Pf1N1B4_2268 at 2586.046 kb on - strand, within Pf1N1B4_2268 at 2586.046 kb on - strand, within Pf1N1B4_2268 at 2586.046 kb on - strand, within Pf1N1B4_2268 at 2586.150 kb on + strand, within Pf1N1B4_2268 at 2586.150 kb on + strand, within Pf1N1B4_2268 at 2586.150 kb on + strand, within Pf1N1B4_2268 at 2586.151 kb on - strand, within Pf1N1B4_2268 at 2586.210 kb on + strand, within Pf1N1B4_2268 at 2586.210 kb on + strand, within Pf1N1B4_2268 at 2586.211 kb on - strand, within Pf1N1B4_2268 at 2586.211 kb on - strand, within Pf1N1B4_2268 at 2586.211 kb on - strand, within Pf1N1B4_2268 at 2586.211 kb on - strand, within Pf1N1B4_2268 at 2586.258 kb on + strand, within Pf1N1B4_2268 at 2586.258 kb on + strand, within Pf1N1B4_2268 at 2586.258 kb on + strand, within Pf1N1B4_2268 at 2586.258 kb on + strand, within Pf1N1B4_2268 at 2586.258 kb on + strand at 2586.258 kb on + strand, within Pf1N1B4_2268 at 2586.258 kb on + strand, within Pf1N1B4_2268 at 2586.258 kb on + strand, within Pf1N1B4_2268 at 2586.259 kb on - strand, within Pf1N1B4_2268 at 2586.259 kb on - strand, within Pf1N1B4_2268 at 2586.259 kb on - strand, within Pf1N1B4_2268 at 2586.259 kb on - strand, within Pf1N1B4_2268 at 2586.259 kb on - strand, within Pf1N1B4_2268 at 2586.259 kb on - strand, within Pf1N1B4_2268 at 2586.259 kb on - strand, within Pf1N1B4_2268 at 2586.259 kb on - strand, within Pf1N1B4_2268 at 2586.259 kb on - strand, within Pf1N1B4_2268 at 2586.767 kb on + strand, within Pf1N1B4_2269 at 2586.767 kb on + strand, within Pf1N1B4_2269 at 2586.768 kb on - strand, within Pf1N1B4_2269 at 2586.768 kb on - strand, within Pf1N1B4_2269 at 2586.768 kb on - strand, within Pf1N1B4_2269 at 2586.768 kb on - strand, within Pf1N1B4_2269 at 2586.768 kb on - strand, within Pf1N1B4_2269 at 2586.970 kb on - strand at 2586.977 kb on + strand at 2587.015 kb on - strand at 2587.284 kb on + strand, within Pf1N1B4_2270 at 2587.284 kb on + strand, within Pf1N1B4_2270 at 2587.284 kb on + strand, within Pf1N1B4_2270 at 2587.285 kb on - strand, within Pf1N1B4_2270 at 2587.285 kb on - strand, within Pf1N1B4_2270 at 2587.332 kb on - strand, within Pf1N1B4_2270 at 2587.353 kb on + strand, within Pf1N1B4_2270 at 2587.354 kb on - strand, within Pf1N1B4_2270 at 2587.354 kb on - strand, within Pf1N1B4_2270 at 2587.501 kb on + strand, within Pf1N1B4_2270 at 2587.501 kb on + strand, within Pf1N1B4_2270 at 2587.501 kb on + strand, within Pf1N1B4_2270 at 2587.501 kb on + strand, within Pf1N1B4_2270 at 2587.501 kb on + strand, within Pf1N1B4_2270 at 2587.501 kb on + strand, within Pf1N1B4_2270 at 2587.502 kb on - strand, within Pf1N1B4_2270 at 2587.542 kb on - strand, within Pf1N1B4_2270 at 2587.542 kb on - strand, within Pf1N1B4_2270 at 2587.640 kb on + strand at 2587.640 kb on + strand at 2587.766 kb on - strand at 2587.825 kb on + strand, within Pf1N1B4_2271 at 2587.826 kb on - strand, within Pf1N1B4_2271 at 2587.826 kb on - strand, within Pf1N1B4_2271 at 2587.826 kb on - strand, within Pf1N1B4_2271 at 2587.826 kb on - strand, within Pf1N1B4_2271 at 2587.826 kb on - strand, within Pf1N1B4_2271 at 2588.070 kb on + strand, within Pf1N1B4_2271 at 2588.070 kb on + strand, within Pf1N1B4_2271 at 2588.070 kb on + strand, within Pf1N1B4_2271 at 2588.070 kb on + strand, within Pf1N1B4_2271 at 2588.071 kb on - strand, within Pf1N1B4_2271 at 2588.071 kb on - strand, within Pf1N1B4_2271 at 2588.071 kb on - strand, within Pf1N1B4_2271 at 2588.173 kb on + strand at 2588.174 kb on - strand at 2588.174 kb on - strand at 2588.174 kb on - strand at 2588.177 kb on + strand at 2588.177 kb on + strand at 2588.177 kb on + strand at 2588.177 kb on + strand at 2588.177 kb on + strand at 2588.177 kb on + strand at 2588.178 kb on - strand at 2588.178 kb on - strand at 2588.178 kb on - strand at 2588.178 kb on - strand at 2588.178 kb on - strand at 2588.238 kb on - strand at 2588.515 kb on + strand, within Pf1N1B4_2272 at 2588.530 kb on - strand, within Pf1N1B4_2272
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 2,586,045 + Pf1N1B4_2268 0.42 +2.7 2,586,045 + Pf1N1B4_2268 0.42 -0.2 2,586,045 + Pf1N1B4_2268 0.42 +1.0 2,586,045 + Pf1N1B4_2268 0.42 +0.6 2,586,045 + Pf1N1B4_2268 0.42 +0.2 2,586,045 + Pf1N1B4_2268 0.42 +0.5 2,586,045 + Pf1N1B4_2268 0.42 +0.4 2,586,045 + Pf1N1B4_2268 0.42 +0.1 2,586,046 - Pf1N1B4_2268 0.42 -0.7 2,586,046 - Pf1N1B4_2268 0.42 -1.3 2,586,046 - Pf1N1B4_2268 0.42 +0.4 2,586,046 - Pf1N1B4_2268 0.42 -0.1 2,586,046 - Pf1N1B4_2268 0.42 +0.1 2,586,046 - Pf1N1B4_2268 0.42 +0.3 2,586,150 + Pf1N1B4_2268 0.58 +1.3 2,586,150 + Pf1N1B4_2268 0.58 +0.8 2,586,150 + Pf1N1B4_2268 0.58 -1.3 2,586,151 - Pf1N1B4_2268 0.58 -0.3 2,586,210 + Pf1N1B4_2268 0.68 +1.1 2,586,210 + Pf1N1B4_2268 0.68 -1.5 2,586,211 - Pf1N1B4_2268 0.68 -0.1 2,586,211 - Pf1N1B4_2268 0.68 -1.5 2,586,211 - Pf1N1B4_2268 0.68 +1.8 2,586,211 - Pf1N1B4_2268 0.68 +1.2 2,586,258 + Pf1N1B4_2268 0.75 -1.7 2,586,258 + Pf1N1B4_2268 0.75 -0.1 2,586,258 + Pf1N1B4_2268 0.75 +1.5 2,586,258 + Pf1N1B4_2268 0.75 +0.8 2,586,258 + -0.5 2,586,258 + Pf1N1B4_2268 0.75 -0.4 2,586,258 + Pf1N1B4_2268 0.75 -0.7 2,586,258 + Pf1N1B4_2268 0.75 -0.5 2,586,259 - Pf1N1B4_2268 0.76 +0.6 2,586,259 - Pf1N1B4_2268 0.76 -0.8 2,586,259 - Pf1N1B4_2268 0.76 +0.1 2,586,259 - Pf1N1B4_2268 0.76 -1.3 2,586,259 - Pf1N1B4_2268 0.76 -1.1 2,586,259 - Pf1N1B4_2268 0.76 +0.4 2,586,259 - Pf1N1B4_2268 0.76 -0.7 2,586,259 - Pf1N1B4_2268 0.76 -0.2 2,586,259 - Pf1N1B4_2268 0.76 +0.5 2,586,767 + Pf1N1B4_2269 0.72 +0.3 2,586,767 + Pf1N1B4_2269 0.72 +0.5 2,586,768 - Pf1N1B4_2269 0.72 -0.8 2,586,768 - Pf1N1B4_2269 0.72 +1.1 2,586,768 - Pf1N1B4_2269 0.72 +0.2 2,586,768 - Pf1N1B4_2269 0.72 -0.2 2,586,768 - Pf1N1B4_2269 0.72 -1.3 2,586,970 - -0.3 2,586,977 + -0.1 2,587,015 - -0.0 2,587,284 + Pf1N1B4_2270 0.43 -0.4 2,587,284 + Pf1N1B4_2270 0.43 +0.1 2,587,284 + Pf1N1B4_2270 0.43 +1.5 2,587,285 - Pf1N1B4_2270 0.43 -1.8 2,587,285 - Pf1N1B4_2270 0.43 +1.5 2,587,332 - Pf1N1B4_2270 0.51 -1.5 2,587,353 + Pf1N1B4_2270 0.55 -1.3 2,587,354 - Pf1N1B4_2270 0.55 -0.3 2,587,354 - Pf1N1B4_2270 0.55 -0.8 2,587,501 + Pf1N1B4_2270 0.80 -0.3 2,587,501 + Pf1N1B4_2270 0.80 -1.3 2,587,501 + Pf1N1B4_2270 0.80 -1.8 2,587,501 + Pf1N1B4_2270 0.80 +0.9 2,587,501 + Pf1N1B4_2270 0.80 -0.5 2,587,501 + Pf1N1B4_2270 0.80 +0.9 2,587,502 - Pf1N1B4_2270 0.80 -0.8 2,587,542 - Pf1N1B4_2270 0.87 -1.1 2,587,542 - Pf1N1B4_2270 0.87 -1.8 2,587,640 + +0.2 2,587,640 + +0.3 2,587,766 - +1.1 2,587,825 + Pf1N1B4_2271 0.17 -0.6 2,587,826 - Pf1N1B4_2271 0.17 -0.1 2,587,826 - Pf1N1B4_2271 0.17 -0.4 2,587,826 - Pf1N1B4_2271 0.17 -0.3 2,587,826 - Pf1N1B4_2271 0.17 +1.3 2,587,826 - Pf1N1B4_2271 0.17 +0.9 2,588,070 + Pf1N1B4_2271 0.74 +1.8 2,588,070 + Pf1N1B4_2271 0.74 -1.7 2,588,070 + Pf1N1B4_2271 0.74 -0.9 2,588,070 + Pf1N1B4_2271 0.74 -1.1 2,588,071 - Pf1N1B4_2271 0.74 +0.5 2,588,071 - Pf1N1B4_2271 0.74 +0.8 2,588,071 - Pf1N1B4_2271 0.74 +0.9 2,588,173 + -1.1 2,588,174 - -0.2 2,588,174 - +0.3 2,588,174 - +1.0 2,588,177 + +0.9 2,588,177 + +2.1 2,588,177 + -0.8 2,588,177 + +0.3 2,588,177 + -0.9 2,588,177 + +1.3 2,588,178 - -0.5 2,588,178 - +1.3 2,588,178 - +0.6 2,588,178 - +0.8 2,588,178 - +0.3 2,588,238 - +0.7 2,588,515 + Pf1N1B4_2272 0.22 -0.3 2,588,530 - Pf1N1B4_2272 0.23 +0.5
Or see this region's nucleotide sequence