Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2050 and Pf1N1B4_2051 are separated by 192 nucleotides Pf1N1B4_2051 and Pf1N1B4_2052 are separated by 99 nucleotides
Pf1N1B4_2050 - Rubredoxin-NAD(+) reductase (EC 1.18.1.1), at 2,336,402 to 2,337,550
_2050
Pf1N1B4_2051 - DNA-binding protein HU-alpha, at 2,337,743 to 2,338,018
_2051
Pf1N1B4_2052 - hypothetical protein, at 2,338,118 to 2,338,327
_2052
Position (kb)
2338
2339 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2337.212 kb on + strand, within Pf1N1B4_2050 at 2337.212 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.258 kb on - strand, within Pf1N1B4_2050 at 2337.258 kb on - strand, within Pf1N1B4_2050 at 2337.258 kb on - strand, within Pf1N1B4_2050 at 2337.258 kb on - strand, within Pf1N1B4_2050 at 2337.317 kb on - strand, within Pf1N1B4_2050 at 2337.317 kb on - strand, within Pf1N1B4_2050 at 2337.329 kb on + strand, within Pf1N1B4_2050 at 2337.472 kb on - strand at 2337.479 kb on + strand at 2337.548 kb on + strand at 2337.549 kb on - strand at 2337.552 kb on + strand at 2337.622 kb on - strand at 2337.659 kb on + strand at 2337.710 kb on + strand at 2337.710 kb on + strand at 2337.711 kb on - strand at 2337.711 kb on - strand at 2337.711 kb on - strand at 2337.711 kb on - strand at 2337.711 kb on - strand at 2337.741 kb on + strand at 2337.741 kb on + strand at 2337.741 kb on + strand at 2337.748 kb on + strand at 2338.016 kb on + strand at 2338.139 kb on + strand, within Pf1N1B4_2052 at 2338.179 kb on + strand, within Pf1N1B4_2052 at 2338.179 kb on + strand, within Pf1N1B4_2052 at 2338.180 kb on - strand, within Pf1N1B4_2052 at 2338.180 kb on - strand, within Pf1N1B4_2052 at 2338.257 kb on + strand, within Pf1N1B4_2052 at 2338.257 kb on + strand, within Pf1N1B4_2052 at 2338.258 kb on - strand, within Pf1N1B4_2052 at 2338.258 kb on - strand, within Pf1N1B4_2052 at 2338.258 kb on - strand, within Pf1N1B4_2052 at 2338.266 kb on + strand, within Pf1N1B4_2052 at 2338.281 kb on - strand, within Pf1N1B4_2052 at 2338.281 kb on - strand, within Pf1N1B4_2052 at 2338.281 kb on - strand, within Pf1N1B4_2052 at 2338.281 kb on - strand, within Pf1N1B4_2052 at 2338.281 kb on - strand, within Pf1N1B4_2052 at 2338.361 kb on + strand at 2338.362 kb on - strand at 2338.415 kb on + strand at 2338.416 kb on - strand at 2338.416 kb on - strand at 2338.416 kb on - strand at 2338.416 kb on - strand at 2338.416 kb on - strand at 2338.416 kb on - strand at 2338.461 kb on + strand at 2338.461 kb on + strand at 2338.461 kb on + strand at 2338.462 kb on - strand at 2338.462 kb on - strand at 2338.462 kb on - strand at 2338.462 kb on - strand at 2338.462 kb on - strand at 2338.462 kb on - strand at 2338.462 kb on - strand at 2338.495 kb on + strand at 2338.568 kb on + strand at 2338.569 kb on - strand at 2338.569 kb on - strand at 2338.736 kb on + strand at 2338.737 kb on - strand at 2338.758 kb on - strand at 2338.826 kb on + strand at 2339.086 kb on - strand at 2339.097 kb on - strand at 2339.101 kb on + strand at 2339.101 kb on + strand at 2339.101 kb on + strand at 2339.101 kb on + strand at 2339.102 kb on - strand at 2339.102 kb on - strand at 2339.102 kb on - strand at 2339.102 kb on - strand at 2339.102 kb on - strand at 2339.110 kb on - strand at 2339.114 kb on - strand at 2339.114 kb on - strand at 2339.134 kb on + strand at 2339.148 kb on - strand at 2339.148 kb on - strand at 2339.148 kb on - strand at 2339.173 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 2,337,212 + Pf1N1B4_2050 0.70 -0.9 2,337,212 + Pf1N1B4_2050 0.70 +0.1 2,337,257 + Pf1N1B4_2050 0.74 +1.4 2,337,257 + Pf1N1B4_2050 0.74 +1.0 2,337,257 + Pf1N1B4_2050 0.74 +0.6 2,337,257 + Pf1N1B4_2050 0.74 +0.4 2,337,257 + Pf1N1B4_2050 0.74 +0.5 2,337,257 + Pf1N1B4_2050 0.74 -1.4 2,337,257 + Pf1N1B4_2050 0.74 +1.4 2,337,258 - Pf1N1B4_2050 0.74 -1.4 2,337,258 - Pf1N1B4_2050 0.74 +0.1 2,337,258 - Pf1N1B4_2050 0.74 +1.1 2,337,258 - Pf1N1B4_2050 0.74 -1.2 2,337,317 - Pf1N1B4_2050 0.80 +0.1 2,337,317 - Pf1N1B4_2050 0.80 +0.1 2,337,329 + Pf1N1B4_2050 0.81 +0.6 2,337,472 - +0.2 2,337,479 + +0.6 2,337,548 + +0.1 2,337,549 - +0.1 2,337,552 + +0.1 2,337,622 - +0.5 2,337,659 + -0.4 2,337,710 + -0.7 2,337,710 + +0.2 2,337,711 - -0.4 2,337,711 - +2.3 2,337,711 - -0.1 2,337,711 - +1.1 2,337,711 - +0.5 2,337,741 + +0.1 2,337,741 + +0.3 2,337,741 + +0.0 2,337,748 + -1.4 2,338,016 + -1.7 2,338,139 + Pf1N1B4_2052 0.10 -0.4 2,338,179 + Pf1N1B4_2052 0.29 +0.6 2,338,179 + Pf1N1B4_2052 0.29 -0.4 2,338,180 - Pf1N1B4_2052 0.30 -2.0 2,338,180 - Pf1N1B4_2052 0.30 -0.4 2,338,257 + Pf1N1B4_2052 0.66 +0.4 2,338,257 + Pf1N1B4_2052 0.66 +1.1 2,338,258 - Pf1N1B4_2052 0.67 -1.4 2,338,258 - Pf1N1B4_2052 0.67 +0.5 2,338,258 - Pf1N1B4_2052 0.67 -0.5 2,338,266 + Pf1N1B4_2052 0.70 -1.1 2,338,281 - Pf1N1B4_2052 0.78 +0.9 2,338,281 - Pf1N1B4_2052 0.78 -0.4 2,338,281 - Pf1N1B4_2052 0.78 +1.9 2,338,281 - Pf1N1B4_2052 0.78 -0.9 2,338,281 - Pf1N1B4_2052 0.78 -0.8 2,338,361 + +0.7 2,338,362 - -1.5 2,338,415 + +0.1 2,338,416 - +0.9 2,338,416 - +1.2 2,338,416 - +0.2 2,338,416 - +0.2 2,338,416 - +0.1 2,338,416 - -1.7 2,338,461 + -1.0 2,338,461 + -1.5 2,338,461 + +0.8 2,338,462 - +0.3 2,338,462 - +0.8 2,338,462 - -1.8 2,338,462 - -0.0 2,338,462 - -0.4 2,338,462 - +0.8 2,338,462 - +1.2 2,338,495 + -1.2 2,338,568 + +1.6 2,338,569 - +0.4 2,338,569 - +2.6 2,338,736 + +3.2 2,338,737 - +0.1 2,338,758 - +2.3 2,338,826 + +0.4 2,339,086 - +1.1 2,339,097 - -1.4 2,339,101 + +1.4 2,339,101 + -0.9 2,339,101 + +0.8 2,339,101 + +0.5 2,339,102 - +1.1 2,339,102 - +1.6 2,339,102 - +0.1 2,339,102 - +0.2 2,339,102 - +0.6 2,339,110 - +0.4 2,339,114 - +0.6 2,339,114 - -0.3 2,339,134 + -0.4 2,339,148 - -1.8 2,339,148 - +1.2 2,339,148 - +0.1 2,339,173 - +0.3
Or see this region's nucleotide sequence