Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1993

Experiment: Sisomicin 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1991 and Pf1N1B4_1992 overlap by 4 nucleotidesPf1N1B4_1992 and Pf1N1B4_1993 are separated by 127 nucleotidesPf1N1B4_1993 and Pf1N1B4_1994 are separated by 28 nucleotides Pf1N1B4_1991 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 2,276,193 to 2,277,401 _1991 Pf1N1B4_1992 - Glutamine synthetase adenylyltransferase, at 2,277,398 to 2,277,775 _1992 Pf1N1B4_1993 - C4-type zinc finger protein, DksA/TraR family, at 2,277,903 to 2,278,307 _1993 Pf1N1B4_1994 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 2,278,336 to 2,279,544 _1994 Position (kb) 2277 2278 2279Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2276.941 kb on - strand, within Pf1N1B4_1991at 2277.196 kb on + strand, within Pf1N1B4_1991at 2277.360 kb on + strandat 2277.360 kb on + strandat 2277.360 kb on + strandat 2277.361 kb on - strandat 2277.361 kb on - strandat 2277.493 kb on - strand, within Pf1N1B4_1992at 2277.493 kb on - strand, within Pf1N1B4_1992at 2277.588 kb on - strand, within Pf1N1B4_1992at 2277.606 kb on + strand, within Pf1N1B4_1992at 2277.606 kb on + strand, within Pf1N1B4_1992at 2277.606 kb on + strand, within Pf1N1B4_1992at 2277.606 kb on + strand, within Pf1N1B4_1992at 2277.606 kb on + strand, within Pf1N1B4_1992at 2277.607 kb on - strand, within Pf1N1B4_1992at 2277.607 kb on - strand, within Pf1N1B4_1992at 2277.607 kb on - strand, within Pf1N1B4_1992at 2277.607 kb on - strand, within Pf1N1B4_1992at 2277.607 kb on - strand, within Pf1N1B4_1992at 2277.607 kb on - strand, within Pf1N1B4_1992at 2277.607 kb on - strand, within Pf1N1B4_1992at 2277.607 kb on - strand, within Pf1N1B4_1992at 2277.607 kb on - strand, within Pf1N1B4_1992at 2277.607 kb on - strand, within Pf1N1B4_1992at 2277.636 kb on + strand, within Pf1N1B4_1992at 2277.636 kb on + strand, within Pf1N1B4_1992at 2277.656 kb on + strand, within Pf1N1B4_1992at 2277.656 kb on + strand, within Pf1N1B4_1992at 2277.656 kb on + strand, within Pf1N1B4_1992at 2277.657 kb on - strand, within Pf1N1B4_1992at 2277.657 kb on - strand, within Pf1N1B4_1992at 2277.657 kb on - strand, within Pf1N1B4_1992at 2277.657 kb on - strand, within Pf1N1B4_1992at 2277.657 kb on - strand, within Pf1N1B4_1992at 2277.802 kb on + strandat 2277.802 kb on + strandat 2277.803 kb on - strandat 2277.803 kb on - strandat 2277.828 kb on + strandat 2277.829 kb on - strandat 2277.942 kb on + strandat 2277.942 kb on + strandat 2277.943 kb on - strandat 2277.943 kb on - strandat 2277.943 kb on - strandat 2277.943 kb on - strandat 2278.253 kb on + strand, within Pf1N1B4_1993at 2278.253 kb on + strand, within Pf1N1B4_1993at 2278.330 kb on + strandat 2278.331 kb on - strandat 2278.331 kb on - strandat 2278.393 kb on - strandat 2278.401 kb on + strandat 2278.401 kb on + strandat 2278.402 kb on - strandat 2278.405 kb on + strandat 2278.405 kb on + strandat 2278.405 kb on + strandat 2278.405 kb on + strandat 2278.410 kb on - strandat 2278.413 kb on - strandat 2278.413 kb on - strandat 2278.712 kb on + strand, within Pf1N1B4_1994at 2278.712 kb on + strand, within Pf1N1B4_1994at 2278.712 kb on + strand, within Pf1N1B4_1994at 2278.713 kb on - strand, within Pf1N1B4_1994at 2278.721 kb on + strand, within Pf1N1B4_1994at 2278.769 kb on - strand, within Pf1N1B4_1994at 2278.769 kb on - strand, within Pf1N1B4_1994at 2279.096 kb on - strand, within Pf1N1B4_1994at 2279.206 kb on + strand, within Pf1N1B4_1994at 2279.206 kb on + strand, within Pf1N1B4_1994at 2279.207 kb on - strand, within Pf1N1B4_1994at 2279.207 kb on - strand, within Pf1N1B4_1994

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml
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2,276,941 - Pf1N1B4_1991 0.62 +3.2
2,277,196 + Pf1N1B4_1991 0.83 -0.4
2,277,360 + +0.1
2,277,360 + +1.6
2,277,360 + +0.5
2,277,361 - +2.5
2,277,361 - +0.3
2,277,493 - Pf1N1B4_1992 0.25 +0.8
2,277,493 - Pf1N1B4_1992 0.25 -1.2
2,277,588 - Pf1N1B4_1992 0.50 +1.1
2,277,606 + Pf1N1B4_1992 0.55 -1.5
2,277,606 + Pf1N1B4_1992 0.55 +0.4
2,277,606 + Pf1N1B4_1992 0.55 -2.9
2,277,606 + Pf1N1B4_1992 0.55 -0.7
2,277,606 + Pf1N1B4_1992 0.55 +1.1
2,277,607 - Pf1N1B4_1992 0.55 +1.6
2,277,607 - Pf1N1B4_1992 0.55 +1.3
2,277,607 - Pf1N1B4_1992 0.55 -0.5
2,277,607 - Pf1N1B4_1992 0.55 +0.9
2,277,607 - Pf1N1B4_1992 0.55 +0.4
2,277,607 - Pf1N1B4_1992 0.55 -0.7
2,277,607 - Pf1N1B4_1992 0.55 +0.5
2,277,607 - Pf1N1B4_1992 0.55 -2.1
2,277,607 - Pf1N1B4_1992 0.55 +0.6
2,277,607 - Pf1N1B4_1992 0.55 +1.1
2,277,636 + Pf1N1B4_1992 0.63 +0.6
2,277,636 + Pf1N1B4_1992 0.63 +1.6
2,277,656 + Pf1N1B4_1992 0.68 -1.2
2,277,656 + Pf1N1B4_1992 0.68 -0.2
2,277,656 + Pf1N1B4_1992 0.68 +2.1
2,277,657 - Pf1N1B4_1992 0.69 +0.6
2,277,657 - Pf1N1B4_1992 0.69 -0.9
2,277,657 - Pf1N1B4_1992 0.69 +1.6
2,277,657 - Pf1N1B4_1992 0.69 -0.0
2,277,657 - Pf1N1B4_1992 0.69 +1.3
2,277,802 + +0.1
2,277,802 + +0.1
2,277,803 - -1.2
2,277,803 - -1.2
2,277,828 + -0.4
2,277,829 - +0.8
2,277,942 + -0.1
2,277,942 + +0.6
2,277,943 - +0.3
2,277,943 - -0.7
2,277,943 - -0.4
2,277,943 - +0.1
2,278,253 + Pf1N1B4_1993 0.86 +0.1
2,278,253 + Pf1N1B4_1993 0.86 -1.1
2,278,330 + +0.1
2,278,331 - +2.6
2,278,331 - +1.2
2,278,393 - -1.0
2,278,401 + +2.0
2,278,401 + -0.6
2,278,402 - +0.1
2,278,405 + +0.1
2,278,405 + -2.6
2,278,405 + +0.6
2,278,405 + +0.8
2,278,410 - -0.4
2,278,413 - +0.1
2,278,413 - -0.4
2,278,712 + Pf1N1B4_1994 0.31 +0.4
2,278,712 + Pf1N1B4_1994 0.31 -0.9
2,278,712 + Pf1N1B4_1994 0.31 -0.1
2,278,713 - Pf1N1B4_1994 0.31 +0.1
2,278,721 + Pf1N1B4_1994 0.32 +0.6
2,278,769 - Pf1N1B4_1994 0.36 +1.0
2,278,769 - Pf1N1B4_1994 0.36 +2.1
2,279,096 - Pf1N1B4_1994 0.63 +0.9
2,279,206 + Pf1N1B4_1994 0.72 -0.2
2,279,206 + Pf1N1B4_1994 0.72 +1.4
2,279,207 - Pf1N1B4_1994 0.72 +0.4
2,279,207 - Pf1N1B4_1994 0.72 -0.2

Or see this region's nucleotide sequence