Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1989 and Pf1N1B4_1990 overlap by 4 nucleotides Pf1N1B4_1990 and Pf1N1B4_1991 are separated by 86 nucleotides Pf1N1B4_1991 and Pf1N1B4_1992 overlap by 4 nucleotides Pf1N1B4_1992 and Pf1N1B4_1993 are separated by 127 nucleotides Pf1N1B4_1993 and Pf1N1B4_1994 are separated by 28 nucleotides
Pf1N1B4_1989 - Zinc ABC transporter, ATP-binding protein ZnuC, at 2,274,545 to 2,275,213
_1989
Pf1N1B4_1990 - GTP cyclohydrolase I (EC 3.5.4.16) type 2, at 2,275,210 to 2,276,106
_1990
Pf1N1B4_1991 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 2,276,193 to 2,277,401
_1991
Pf1N1B4_1992 - Glutamine synthetase adenylyltransferase, at 2,277,398 to 2,277,775
_1992
Pf1N1B4_1993 - C4-type zinc finger protein, DksA/TraR family, at 2,277,903 to 2,278,307
_1993
Pf1N1B4_1994 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 2,278,336 to 2,279,544
_1994
Position (kb)
2276
2277
2278 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2275.485 kb on - strand, within Pf1N1B4_1990 at 2275.485 kb on - strand, within Pf1N1B4_1990 at 2275.673 kb on + strand, within Pf1N1B4_1990 at 2275.674 kb on - strand, within Pf1N1B4_1990 at 2275.792 kb on + strand, within Pf1N1B4_1990 at 2275.792 kb on + strand at 2275.792 kb on + strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.793 kb on - strand, within Pf1N1B4_1990 at 2275.815 kb on - strand, within Pf1N1B4_1990 at 2275.919 kb on + strand, within Pf1N1B4_1990 at 2275.919 kb on + strand, within Pf1N1B4_1990 at 2275.920 kb on - strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.932 kb on + strand, within Pf1N1B4_1990 at 2275.933 kb on - strand, within Pf1N1B4_1990 at 2275.933 kb on - strand, within Pf1N1B4_1990 at 2275.933 kb on - strand, within Pf1N1B4_1990 at 2275.933 kb on - strand, within Pf1N1B4_1990 at 2276.343 kb on + strand, within Pf1N1B4_1991 at 2276.343 kb on + strand, within Pf1N1B4_1991 at 2276.343 kb on + strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.344 kb on - strand, within Pf1N1B4_1991 at 2276.426 kb on - strand, within Pf1N1B4_1991 at 2276.438 kb on + strand, within Pf1N1B4_1991 at 2276.439 kb on - strand, within Pf1N1B4_1991 at 2276.941 kb on - strand, within Pf1N1B4_1991 at 2277.196 kb on + strand, within Pf1N1B4_1991 at 2277.360 kb on + strand at 2277.360 kb on + strand at 2277.360 kb on + strand at 2277.361 kb on - strand at 2277.361 kb on - strand at 2277.493 kb on - strand, within Pf1N1B4_1992 at 2277.493 kb on - strand, within Pf1N1B4_1992 at 2277.588 kb on - strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.606 kb on + strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.607 kb on - strand, within Pf1N1B4_1992 at 2277.636 kb on + strand, within Pf1N1B4_1992 at 2277.636 kb on + strand, within Pf1N1B4_1992 at 2277.656 kb on + strand, within Pf1N1B4_1992 at 2277.656 kb on + strand, within Pf1N1B4_1992 at 2277.656 kb on + strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.657 kb on - strand, within Pf1N1B4_1992 at 2277.802 kb on + strand at 2277.802 kb on + strand at 2277.803 kb on - strand at 2277.803 kb on - strand at 2277.828 kb on + strand at 2277.829 kb on - strand at 2277.942 kb on + strand at 2277.942 kb on + strand at 2277.943 kb on - strand at 2277.943 kb on - strand at 2277.943 kb on - strand at 2277.943 kb on - strand at 2278.253 kb on + strand, within Pf1N1B4_1993 at 2278.253 kb on + strand, within Pf1N1B4_1993 at 2278.330 kb on + strand at 2278.331 kb on - strand at 2278.331 kb on - strand at 2278.393 kb on - strand at 2278.401 kb on + strand at 2278.401 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 2,275,485 - Pf1N1B4_1990 0.31 +1.0 2,275,485 - Pf1N1B4_1990 0.31 -0.5 2,275,673 + Pf1N1B4_1990 0.52 +1.6 2,275,674 - Pf1N1B4_1990 0.52 +0.1 2,275,792 + Pf1N1B4_1990 0.65 +0.8 2,275,792 + +1.6 2,275,792 + Pf1N1B4_1990 0.65 -1.4 2,275,793 - Pf1N1B4_1990 0.65 +1.0 2,275,793 - Pf1N1B4_1990 0.65 -0.0 2,275,793 - Pf1N1B4_1990 0.65 -1.2 2,275,793 - Pf1N1B4_1990 0.65 +0.7 2,275,793 - Pf1N1B4_1990 0.65 -1.2 2,275,815 - Pf1N1B4_1990 0.67 +0.1 2,275,919 + Pf1N1B4_1990 0.79 -0.4 2,275,919 + Pf1N1B4_1990 0.79 +0.8 2,275,920 - Pf1N1B4_1990 0.79 -0.7 2,275,932 + Pf1N1B4_1990 0.80 -0.7 2,275,932 + Pf1N1B4_1990 0.80 +0.9 2,275,932 + Pf1N1B4_1990 0.80 -0.4 2,275,932 + Pf1N1B4_1990 0.80 -0.7 2,275,932 + Pf1N1B4_1990 0.80 +0.3 2,275,932 + Pf1N1B4_1990 0.80 +1.4 2,275,932 + Pf1N1B4_1990 0.80 -0.7 2,275,932 + Pf1N1B4_1990 0.80 -2.1 2,275,932 + Pf1N1B4_1990 0.80 -0.1 2,275,932 + Pf1N1B4_1990 0.80 -0.9 2,275,932 + Pf1N1B4_1990 0.80 +0.1 2,275,932 + Pf1N1B4_1990 0.80 +1.0 2,275,932 + Pf1N1B4_1990 0.80 +0.1 2,275,932 + Pf1N1B4_1990 0.80 +1.4 2,275,932 + Pf1N1B4_1990 0.80 +0.1 2,275,933 - Pf1N1B4_1990 0.81 +0.3 2,275,933 - Pf1N1B4_1990 0.81 +1.6 2,275,933 - Pf1N1B4_1990 0.81 +0.9 2,275,933 - Pf1N1B4_1990 0.81 +0.1 2,276,343 + Pf1N1B4_1991 0.12 +1.2 2,276,343 + Pf1N1B4_1991 0.12 +0.6 2,276,343 + Pf1N1B4_1991 0.12 +0.8 2,276,344 - Pf1N1B4_1991 0.12 -0.7 2,276,344 - Pf1N1B4_1991 0.12 +1.6 2,276,344 - Pf1N1B4_1991 0.12 +0.2 2,276,344 - Pf1N1B4_1991 0.12 +1.4 2,276,344 - Pf1N1B4_1991 0.12 -0.8 2,276,344 - Pf1N1B4_1991 0.12 +0.4 2,276,344 - Pf1N1B4_1991 0.12 -2.2 2,276,426 - Pf1N1B4_1991 0.19 -0.3 2,276,438 + Pf1N1B4_1991 0.20 +1.4 2,276,439 - Pf1N1B4_1991 0.20 +0.4 2,276,941 - Pf1N1B4_1991 0.62 +3.2 2,277,196 + Pf1N1B4_1991 0.83 -0.4 2,277,360 + +0.1 2,277,360 + +1.6 2,277,360 + +0.5 2,277,361 - +2.5 2,277,361 - +0.3 2,277,493 - Pf1N1B4_1992 0.25 +0.8 2,277,493 - Pf1N1B4_1992 0.25 -1.2 2,277,588 - Pf1N1B4_1992 0.50 +1.1 2,277,606 + Pf1N1B4_1992 0.55 -1.5 2,277,606 + Pf1N1B4_1992 0.55 +0.4 2,277,606 + Pf1N1B4_1992 0.55 -2.9 2,277,606 + Pf1N1B4_1992 0.55 -0.7 2,277,606 + Pf1N1B4_1992 0.55 +1.1 2,277,607 - Pf1N1B4_1992 0.55 +1.6 2,277,607 - Pf1N1B4_1992 0.55 +1.3 2,277,607 - Pf1N1B4_1992 0.55 -0.5 2,277,607 - Pf1N1B4_1992 0.55 +0.9 2,277,607 - Pf1N1B4_1992 0.55 +0.4 2,277,607 - Pf1N1B4_1992 0.55 -0.7 2,277,607 - Pf1N1B4_1992 0.55 +0.5 2,277,607 - Pf1N1B4_1992 0.55 -2.1 2,277,607 - Pf1N1B4_1992 0.55 +0.6 2,277,607 - Pf1N1B4_1992 0.55 +1.1 2,277,636 + Pf1N1B4_1992 0.63 +0.6 2,277,636 + Pf1N1B4_1992 0.63 +1.6 2,277,656 + Pf1N1B4_1992 0.68 -1.2 2,277,656 + Pf1N1B4_1992 0.68 -0.2 2,277,656 + Pf1N1B4_1992 0.68 +2.1 2,277,657 - Pf1N1B4_1992 0.69 +0.6 2,277,657 - Pf1N1B4_1992 0.69 -0.9 2,277,657 - Pf1N1B4_1992 0.69 +1.6 2,277,657 - Pf1N1B4_1992 0.69 -0.0 2,277,657 - Pf1N1B4_1992 0.69 +1.3 2,277,802 + +0.1 2,277,802 + +0.1 2,277,803 - -1.2 2,277,803 - -1.2 2,277,828 + -0.4 2,277,829 - +0.8 2,277,942 + -0.1 2,277,942 + +0.6 2,277,943 - +0.3 2,277,943 - -0.7 2,277,943 - -0.4 2,277,943 - +0.1 2,278,253 + Pf1N1B4_1993 0.86 +0.1 2,278,253 + Pf1N1B4_1993 0.86 -1.1 2,278,330 + +0.1 2,278,331 - +2.6 2,278,331 - +1.2 2,278,393 - -1.0 2,278,401 + +2.0 2,278,401 + -0.6
Or see this region's nucleotide sequence