Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1613 and Pf1N1B4_1614 are separated by 205 nucleotides Pf1N1B4_1614 and Pf1N1B4_1615 are separated by 55 nucleotides Pf1N1B4_1615 and Pf1N1B4_1616 are separated by 1 nucleotides Pf1N1B4_1616 and Pf1N1B4_1617 are separated by 152 nucleotides
Pf1N1B4_1613 - tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207), at 1,858,329 to 1,858,784
_1613
Pf1N1B4_1614 - Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state), at 1,858,990 to 1,859,475
_1614
Pf1N1B4_1615 - Glutaredoxin 3 (Grx3), at 1,859,531 to 1,859,785
_1615
Pf1N1B4_1616 - FIG136845: Rhodanese-related sulfurtransferase, at 1,859,787 to 1,860,200
_1616
Pf1N1B4_1617 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1), at 1,860,353 to 1,861,882
_1617
Position (kb)
1859
1860 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1858.563 kb on - strand, within Pf1N1B4_1613 at 1858.752 kb on + strand at 1858.783 kb on - strand at 1859.025 kb on + strand at 1859.025 kb on + strand at 1859.025 kb on + strand at 1859.025 kb on + strand at 1859.025 kb on + strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.045 kb on + strand, within Pf1N1B4_1614 at 1859.045 kb on + strand, within Pf1N1B4_1614 at 1859.046 kb on - strand, within Pf1N1B4_1614 at 1859.046 kb on - strand, within Pf1N1B4_1614 at 1859.046 kb on - strand, within Pf1N1B4_1614 at 1859.405 kb on + strand at 1859.406 kb on - strand, within Pf1N1B4_1614 at 1859.406 kb on - strand, within Pf1N1B4_1614 at 1859.406 kb on - strand, within Pf1N1B4_1614 at 1859.406 kb on - strand, within Pf1N1B4_1614 at 1859.406 kb on - strand, within Pf1N1B4_1614 at 1859.406 kb on - strand, within Pf1N1B4_1614 at 1859.503 kb on - strand at 1859.504 kb on + strand at 1859.504 kb on + strand at 1859.504 kb on + strand at 1859.504 kb on + strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.505 kb on - strand at 1859.514 kb on + strand at 1859.515 kb on - strand at 1859.515 kb on - strand at 1859.523 kb on + strand at 1859.523 kb on + strand at 1859.523 kb on + strand at 1859.524 kb on - strand at 1859.635 kb on + strand, within Pf1N1B4_1615 at 1859.635 kb on + strand, within Pf1N1B4_1615 at 1859.636 kb on - strand, within Pf1N1B4_1615 at 1859.636 kb on - strand, within Pf1N1B4_1615 at 1859.636 kb on - strand, within Pf1N1B4_1615 at 1859.728 kb on - strand, within Pf1N1B4_1615 at 1859.728 kb on - strand, within Pf1N1B4_1615 at 1859.728 kb on - strand, within Pf1N1B4_1615 at 1859.767 kb on - strand at 1859.767 kb on - strand at 1859.785 kb on + strand at 1860.222 kb on - strand at 1860.237 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.239 kb on - strand at 1860.249 kb on + strand at 1860.250 kb on - strand at 1860.292 kb on + strand at 1860.292 kb on + strand at 1860.292 kb on + strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.293 kb on - strand at 1860.299 kb on - strand at 1860.299 kb on - strand at 1860.299 kb on - strand at 1860.307 kb on - strand at 1860.307 kb on - strand at 1860.307 kb on - strand at 1860.330 kb on + strand at 1860.330 kb on + strand at 1860.331 kb on - strand at 1860.331 kb on - strand at 1860.358 kb on + strand at 1860.359 kb on - strand at 1860.434 kb on + strand at 1860.434 kb on + strand at 1860.434 kb on + strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.435 kb on - strand at 1860.584 kb on + strand, within Pf1N1B4_1617 at 1860.768 kb on - strand, within Pf1N1B4_1617 at 1860.768 kb on - strand, within Pf1N1B4_1617 at 1860.768 kb on - strand, within Pf1N1B4_1617 at 1860.768 kb on - strand, within Pf1N1B4_1617
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 1,858,563 - Pf1N1B4_1613 0.51 -1.2 1,858,752 + -0.4 1,858,783 - +0.6 1,859,025 + -0.4 1,859,025 + -1.0 1,859,025 + +1.7 1,859,025 + +1.0 1,859,025 + -0.4 1,859,026 - +0.2 1,859,026 - -0.3 1,859,026 - +0.4 1,859,026 - +0.7 1,859,026 - -0.8 1,859,026 - +0.1 1,859,026 - -0.2 1,859,026 - -0.3 1,859,026 - -0.8 1,859,026 - -0.8 1,859,026 - +1.6 1,859,026 - +0.1 1,859,045 + Pf1N1B4_1614 0.11 +0.6 1,859,045 + Pf1N1B4_1614 0.11 -1.0 1,859,046 - Pf1N1B4_1614 0.12 -1.3 1,859,046 - Pf1N1B4_1614 0.12 -0.4 1,859,046 - Pf1N1B4_1614 0.12 -0.8 1,859,405 + +3.1 1,859,406 - Pf1N1B4_1614 0.86 -0.4 1,859,406 - Pf1N1B4_1614 0.86 -0.8 1,859,406 - Pf1N1B4_1614 0.86 +2.1 1,859,406 - Pf1N1B4_1614 0.86 -0.8 1,859,406 - Pf1N1B4_1614 0.86 -1.4 1,859,406 - Pf1N1B4_1614 0.86 +1.1 1,859,503 - -0.4 1,859,504 + -0.8 1,859,504 + -1.1 1,859,504 + +0.3 1,859,504 + -0.8 1,859,505 - +0.2 1,859,505 - -0.2 1,859,505 - -0.2 1,859,505 - +0.1 1,859,505 - +1.0 1,859,505 - -0.2 1,859,505 - +0.8 1,859,505 - +0.6 1,859,514 + +1.0 1,859,515 - -1.1 1,859,515 - +0.2 1,859,523 + +1.6 1,859,523 + +1.0 1,859,523 + -1.6 1,859,524 - +0.3 1,859,635 + Pf1N1B4_1615 0.41 -0.4 1,859,635 + Pf1N1B4_1615 0.41 -0.4 1,859,636 - Pf1N1B4_1615 0.41 -0.8 1,859,636 - Pf1N1B4_1615 0.41 +0.6 1,859,636 - Pf1N1B4_1615 0.41 -1.0 1,859,728 - Pf1N1B4_1615 0.77 -0.0 1,859,728 - Pf1N1B4_1615 0.77 -0.3 1,859,728 - Pf1N1B4_1615 0.77 -1.6 1,859,767 - -0.8 1,859,767 - -0.4 1,859,785 + -0.2 1,860,222 - -1.0 1,860,237 - -2.1 1,860,239 - +1.6 1,860,239 - -0.0 1,860,239 - +0.1 1,860,239 - +0.7 1,860,239 - -0.4 1,860,239 - -0.6 1,860,239 - -1.6 1,860,239 - +0.8 1,860,239 - -0.2 1,860,239 - +1.1 1,860,249 + -0.4 1,860,250 - -0.9 1,860,292 + -1.8 1,860,292 + -0.0 1,860,292 + -1.2 1,860,293 - -1.6 1,860,293 - +0.6 1,860,293 - -0.2 1,860,293 - -0.9 1,860,293 - -0.9 1,860,293 - +1.1 1,860,293 - -0.6 1,860,293 - +0.4 1,860,293 - +1.4 1,860,293 - -1.8 1,860,293 - -1.2 1,860,293 - +0.9 1,860,299 - -1.2 1,860,299 - +0.8 1,860,299 - -1.6 1,860,307 - +1.1 1,860,307 - -0.9 1,860,307 - -0.8 1,860,330 + -1.4 1,860,330 + +0.6 1,860,331 - +1.0 1,860,331 - +0.8 1,860,358 + -0.9 1,860,359 - -0.8 1,860,434 + -0.8 1,860,434 + -0.8 1,860,434 + +0.1 1,860,435 - +1.0 1,860,435 - -1.3 1,860,435 - -0.0 1,860,435 - +1.0 1,860,435 - -2.9 1,860,435 - -1.4 1,860,435 - +0.6 1,860,435 - -0.3 1,860,584 + Pf1N1B4_1617 0.15 -2.6 1,860,768 - Pf1N1B4_1617 0.27 -0.2 1,860,768 - Pf1N1B4_1617 0.27 +0.7 1,860,768 - Pf1N1B4_1617 0.27 +0.1 1,860,768 - Pf1N1B4_1617 0.27 -1.5
Or see this region's nucleotide sequence