Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1610 and Pf1N1B4_1611 overlap by 4 nucleotides Pf1N1B4_1611 and Pf1N1B4_1612 are separated by 630 nucleotides
Pf1N1B4_1610 - Nitrogen regulation protein NtrB (EC 2.7.13.3), at 1,854,741 to 1,855,826
_1610
Pf1N1B4_1611 - Nitrogen regulation protein NR(I), at 1,855,823 to 1,857,259
_1611
Pf1N1B4_1612 - FIG00955429: hypothetical protein, at 1,857,890 to 1,858,330
_1612
Position (kb)
1855
1856
1857
1858 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1854.828 kb on + strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.875 kb on + strand, within Pf1N1B4_1610 at 1855.020 kb on + strand, within Pf1N1B4_1610 at 1855.021 kb on - strand, within Pf1N1B4_1610 at 1855.021 kb on - strand, within Pf1N1B4_1610 at 1855.053 kb on - strand, within Pf1N1B4_1610 at 1855.248 kb on + strand, within Pf1N1B4_1610 at 1855.367 kb on - strand, within Pf1N1B4_1610 at 1855.367 kb on - strand, within Pf1N1B4_1610 at 1855.367 kb on - strand, within Pf1N1B4_1610 at 1855.419 kb on + strand, within Pf1N1B4_1610 at 1855.867 kb on + strand at 1855.868 kb on - strand at 1855.868 kb on - strand at 1855.868 kb on - strand at 1856.106 kb on - strand, within Pf1N1B4_1611 at 1856.126 kb on + strand, within Pf1N1B4_1611 at 1856.126 kb on + strand, within Pf1N1B4_1611 at 1856.127 kb on - strand, within Pf1N1B4_1611 at 1856.127 kb on - strand, within Pf1N1B4_1611 at 1856.233 kb on + strand at 1856.609 kb on + strand, within Pf1N1B4_1611 at 1856.609 kb on + strand, within Pf1N1B4_1611 at 1856.610 kb on - strand, within Pf1N1B4_1611 at 1856.610 kb on - strand, within Pf1N1B4_1611 at 1856.864 kb on + strand, within Pf1N1B4_1611 at 1856.864 kb on + strand, within Pf1N1B4_1611 at 1856.864 kb on + strand, within Pf1N1B4_1611 at 1856.865 kb on - strand, within Pf1N1B4_1611 at 1857.184 kb on + strand at 1857.184 kb on + strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.258 kb on - strand at 1857.262 kb on - strand at 1857.626 kb on + strand at 1857.627 kb on - strand at 1857.627 kb on - strand at 1857.653 kb on + strand at 1857.653 kb on + strand at 1857.653 kb on + strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.708 kb on + strand at 1857.708 kb on + strand at 1857.734 kb on + strand at 1857.734 kb on + strand at 1857.734 kb on + strand at 1857.812 kb on + strand at 1857.812 kb on + strand at 1857.812 kb on + strand at 1857.813 kb on - strand at 1857.813 kb on - strand at 1857.813 kb on - strand at 1857.864 kb on + strand at 1857.865 kb on - strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.892 kb on - strand at 1857.892 kb on - strand at 1857.892 kb on - strand at 1858.079 kb on + strand, within Pf1N1B4_1612 at 1858.080 kb on - strand, within Pf1N1B4_1612 at 1858.080 kb on - strand, within Pf1N1B4_1612
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 1,854,828 + +0.5 1,854,829 - +0.5 1,854,829 - -0.6 1,854,829 - +1.5 1,854,829 - +1.0 1,854,829 - +1.1 1,854,829 - -1.6 1,854,829 - +1.5 1,854,829 - -0.8 1,854,829 - -2.4 1,854,829 - -0.0 1,854,829 - +0.4 1,854,829 - -2.0 1,854,875 + Pf1N1B4_1610 0.12 -0.5 1,855,020 + Pf1N1B4_1610 0.26 +1.0 1,855,021 - Pf1N1B4_1610 0.26 -3.0 1,855,021 - Pf1N1B4_1610 0.26 -0.5 1,855,053 - Pf1N1B4_1610 0.29 -2.0 1,855,248 + Pf1N1B4_1610 0.47 -1.8 1,855,367 - Pf1N1B4_1610 0.58 -1.5 1,855,367 - Pf1N1B4_1610 0.58 -0.5 1,855,367 - Pf1N1B4_1610 0.58 -0.0 1,855,419 + Pf1N1B4_1610 0.62 -1.6 1,855,867 + -0.8 1,855,868 - +0.4 1,855,868 - -2.2 1,855,868 - +0.5 1,856,106 - Pf1N1B4_1611 0.20 -0.5 1,856,126 + Pf1N1B4_1611 0.21 -2.2 1,856,126 + Pf1N1B4_1611 0.21 -1.6 1,856,127 - Pf1N1B4_1611 0.21 -0.6 1,856,127 - Pf1N1B4_1611 0.21 -1.0 1,856,233 + -0.0 1,856,609 + Pf1N1B4_1611 0.55 -1.0 1,856,609 + Pf1N1B4_1611 0.55 -0.9 1,856,610 - Pf1N1B4_1611 0.55 -1.2 1,856,610 - Pf1N1B4_1611 0.55 +1.0 1,856,864 + Pf1N1B4_1611 0.72 -0.3 1,856,864 + Pf1N1B4_1611 0.72 -2.0 1,856,864 + Pf1N1B4_1611 0.72 -0.1 1,856,865 - Pf1N1B4_1611 0.73 +1.0 1,857,184 + +1.1 1,857,184 + -0.6 1,857,185 - -1.0 1,857,185 - +0.1 1,857,185 - -1.0 1,857,185 - -0.0 1,857,185 - +1.8 1,857,257 + +0.9 1,857,257 + +0.8 1,857,257 + +1.1 1,857,257 + -0.1 1,857,257 + -0.5 1,857,257 + -0.2 1,857,258 - -1.0 1,857,262 - +1.2 1,857,626 + -0.7 1,857,627 - +1.6 1,857,627 - -0.8 1,857,653 + +2.6 1,857,653 + -0.0 1,857,653 + -0.6 1,857,654 - -0.0 1,857,654 - -3.1 1,857,654 - -0.0 1,857,654 - +0.6 1,857,654 - +0.3 1,857,708 + +0.8 1,857,708 + -0.0 1,857,734 + -0.4 1,857,734 + -0.8 1,857,734 + +0.7 1,857,812 + -1.0 1,857,812 + -1.8 1,857,812 + -1.4 1,857,813 - -0.1 1,857,813 - +1.0 1,857,813 - -1.6 1,857,864 + -0.4 1,857,865 - +0.2 1,857,891 + +0.2 1,857,891 + -1.4 1,857,891 + -0.8 1,857,891 + -0.8 1,857,891 + +0.4 1,857,891 + +0.4 1,857,891 + +0.6 1,857,892 - -0.3 1,857,892 - -0.4 1,857,892 - +0.3 1,858,079 + Pf1N1B4_1612 0.43 -0.8 1,858,080 - Pf1N1B4_1612 0.43 -1.3 1,858,080 - Pf1N1B4_1612 0.43 +0.8
Or see this region's nucleotide sequence