Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1609 and Pf1N1B4_1610 are separated by 418 nucleotides Pf1N1B4_1610 and Pf1N1B4_1611 overlap by 4 nucleotides
Pf1N1B4_1609 - FIG00953806: hypothetical protein, at 1,853,723 to 1,854,322
_1609
Pf1N1B4_1610 - Nitrogen regulation protein NtrB (EC 2.7.13.3), at 1,854,741 to 1,855,826
_1610
Pf1N1B4_1611 - Nitrogen regulation protein NR(I), at 1,855,823 to 1,857,259
_1611
Position (kb)
1854
1855
1856 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1853.782 kb on + strand at 1853.783 kb on - strand, within Pf1N1B4_1609 at 1853.783 kb on - strand, within Pf1N1B4_1609 at 1853.786 kb on + strand, within Pf1N1B4_1609 at 1853.786 kb on + strand, within Pf1N1B4_1609 at 1853.786 kb on + strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.787 kb on - strand, within Pf1N1B4_1609 at 1853.836 kb on + strand, within Pf1N1B4_1609 at 1853.983 kb on + strand at 1854.341 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.348 kb on - strand at 1854.481 kb on + strand at 1854.482 kb on - strand at 1854.531 kb on + strand at 1854.532 kb on - strand at 1854.534 kb on - strand at 1854.534 kb on - strand at 1854.544 kb on + strand at 1854.557 kb on - strand at 1854.557 kb on - strand at 1854.589 kb on - strand at 1854.589 kb on - strand at 1854.589 kb on - strand at 1854.589 kb on - strand at 1854.648 kb on - strand at 1854.761 kb on - strand at 1854.761 kb on - strand at 1854.761 kb on - strand at 1854.761 kb on - strand at 1854.761 kb on - strand at 1854.772 kb on + strand at 1854.773 kb on - strand at 1854.773 kb on - strand at 1854.773 kb on - strand at 1854.773 kb on - strand at 1854.828 kb on + strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.829 kb on - strand at 1854.875 kb on + strand, within Pf1N1B4_1610 at 1855.020 kb on + strand, within Pf1N1B4_1610 at 1855.021 kb on - strand, within Pf1N1B4_1610 at 1855.021 kb on - strand, within Pf1N1B4_1610 at 1855.053 kb on - strand, within Pf1N1B4_1610 at 1855.248 kb on + strand, within Pf1N1B4_1610 at 1855.367 kb on - strand, within Pf1N1B4_1610 at 1855.367 kb on - strand, within Pf1N1B4_1610 at 1855.367 kb on - strand, within Pf1N1B4_1610 at 1855.419 kb on + strand, within Pf1N1B4_1610 at 1855.867 kb on + strand at 1855.868 kb on - strand at 1855.868 kb on - strand at 1855.868 kb on - strand at 1856.106 kb on - strand, within Pf1N1B4_1611 at 1856.126 kb on + strand, within Pf1N1B4_1611 at 1856.126 kb on + strand, within Pf1N1B4_1611 at 1856.127 kb on - strand, within Pf1N1B4_1611 at 1856.127 kb on - strand, within Pf1N1B4_1611 at 1856.233 kb on + strand at 1856.609 kb on + strand, within Pf1N1B4_1611 at 1856.609 kb on + strand, within Pf1N1B4_1611 at 1856.610 kb on - strand, within Pf1N1B4_1611 at 1856.610 kb on - strand, within Pf1N1B4_1611
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 1,853,782 + -0.0 1,853,783 - Pf1N1B4_1609 0.10 -0.0 1,853,783 - Pf1N1B4_1609 0.10 -1.3 1,853,786 + Pf1N1B4_1609 0.10 -0.0 1,853,786 + Pf1N1B4_1609 0.10 -1.2 1,853,786 + Pf1N1B4_1609 0.10 +2.0 1,853,787 - Pf1N1B4_1609 0.11 -0.8 1,853,787 - Pf1N1B4_1609 0.11 -1.2 1,853,787 - Pf1N1B4_1609 0.11 +0.5 1,853,787 - Pf1N1B4_1609 0.11 +1.0 1,853,787 - Pf1N1B4_1609 0.11 -1.6 1,853,836 + Pf1N1B4_1609 0.19 -2.4 1,853,983 + -0.0 1,854,341 - +0.6 1,854,348 - +0.1 1,854,348 - +1.1 1,854,348 - -0.0 1,854,348 - +1.0 1,854,348 - +1.5 1,854,348 - +1.7 1,854,348 - +0.2 1,854,348 - +3.1 1,854,348 - -2.9 1,854,348 - -0.0 1,854,348 - -0.0 1,854,481 + +1.5 1,854,482 - +0.7 1,854,531 + -0.0 1,854,532 - +1.0 1,854,534 - -1.5 1,854,534 - +1.3 1,854,544 + +1.1 1,854,557 - -0.0 1,854,557 - -0.5 1,854,589 - +1.0 1,854,589 - +2.0 1,854,589 - +0.1 1,854,589 - +1.2 1,854,648 - -0.5 1,854,761 - +0.4 1,854,761 - -0.2 1,854,761 - +0.2 1,854,761 - -0.0 1,854,761 - +0.5 1,854,772 + -1.3 1,854,773 - -1.5 1,854,773 - +0.5 1,854,773 - -0.8 1,854,773 - +0.6 1,854,828 + +0.5 1,854,829 - +0.5 1,854,829 - -0.6 1,854,829 - +1.5 1,854,829 - +1.0 1,854,829 - +1.1 1,854,829 - -1.6 1,854,829 - +1.5 1,854,829 - -0.8 1,854,829 - -2.4 1,854,829 - -0.0 1,854,829 - +0.4 1,854,829 - -2.0 1,854,875 + Pf1N1B4_1610 0.12 -0.5 1,855,020 + Pf1N1B4_1610 0.26 +1.0 1,855,021 - Pf1N1B4_1610 0.26 -3.0 1,855,021 - Pf1N1B4_1610 0.26 -0.5 1,855,053 - Pf1N1B4_1610 0.29 -2.0 1,855,248 + Pf1N1B4_1610 0.47 -1.8 1,855,367 - Pf1N1B4_1610 0.58 -1.5 1,855,367 - Pf1N1B4_1610 0.58 -0.5 1,855,367 - Pf1N1B4_1610 0.58 -0.0 1,855,419 + Pf1N1B4_1610 0.62 -1.6 1,855,867 + -0.8 1,855,868 - +0.4 1,855,868 - -2.2 1,855,868 - +0.5 1,856,106 - Pf1N1B4_1611 0.20 -0.5 1,856,126 + Pf1N1B4_1611 0.21 -2.2 1,856,126 + Pf1N1B4_1611 0.21 -1.6 1,856,127 - Pf1N1B4_1611 0.21 -0.6 1,856,127 - Pf1N1B4_1611 0.21 -1.0 1,856,233 + -0.0 1,856,609 + Pf1N1B4_1611 0.55 -1.0 1,856,609 + Pf1N1B4_1611 0.55 -0.9 1,856,610 - Pf1N1B4_1611 0.55 -1.2 1,856,610 - Pf1N1B4_1611 0.55 +1.0
Or see this region's nucleotide sequence