Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1572 and Pf1N1B4_1573 overlap by 8 nucleotides Pf1N1B4_1573 and Pf1N1B4_1574 are separated by 351 nucleotides Pf1N1B4_1574 and Pf1N1B4_1575 are separated by 50 nucleotides
Pf1N1B4_1572 - Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-), at 1,808,912 to 1,811,482
_1572
Pf1N1B4_1573 - Glucans biosynthesis protein G precursor, at 1,811,475 to 1,813,250
_1573
Pf1N1B4_1574 - D-tyrosyl-tRNA(Tyr) deacylase (EC 3.6.1.n1), at 1,813,602 to 1,813,985
_1574
Pf1N1B4_1575 - Proline iminopeptidase (EC 3.4.11.5), at 1,814,036 to 1,815,007
_1575
Position (kb)
1811
1812
1813
1814 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1810.501 kb on + strand, within Pf1N1B4_1572 at 1810.508 kb on + strand, within Pf1N1B4_1572 at 1810.509 kb on - strand, within Pf1N1B4_1572 at 1810.530 kb on + strand, within Pf1N1B4_1572 at 1810.794 kb on + strand, within Pf1N1B4_1572 at 1810.794 kb on + strand, within Pf1N1B4_1572 at 1810.882 kb on + strand, within Pf1N1B4_1572 at 1810.883 kb on - strand, within Pf1N1B4_1572 at 1810.883 kb on - strand, within Pf1N1B4_1572 at 1810.909 kb on - strand, within Pf1N1B4_1572 at 1810.909 kb on - strand, within Pf1N1B4_1572 at 1810.909 kb on - strand, within Pf1N1B4_1572 at 1811.007 kb on + strand, within Pf1N1B4_1572 at 1811.007 kb on + strand, within Pf1N1B4_1572 at 1811.007 kb on + strand, within Pf1N1B4_1572 at 1811.008 kb on - strand, within Pf1N1B4_1572 at 1811.055 kb on + strand, within Pf1N1B4_1572 at 1811.465 kb on + strand at 1811.465 kb on + strand at 1811.465 kb on + strand at 1811.465 kb on + strand at 1811.465 kb on + strand at 1811.477 kb on - strand at 1811.477 kb on - strand at 1811.498 kb on - strand at 1811.498 kb on - strand at 1811.956 kb on + strand, within Pf1N1B4_1573 at 1811.957 kb on - strand, within Pf1N1B4_1573 at 1811.957 kb on - strand, within Pf1N1B4_1573 at 1812.149 kb on + strand, within Pf1N1B4_1573 at 1812.150 kb on - strand, within Pf1N1B4_1573 at 1812.271 kb on + strand, within Pf1N1B4_1573 at 1812.272 kb on - strand, within Pf1N1B4_1573 at 1812.437 kb on - strand, within Pf1N1B4_1573 at 1812.570 kb on + strand, within Pf1N1B4_1573 at 1812.571 kb on - strand, within Pf1N1B4_1573 at 1812.571 kb on - strand, within Pf1N1B4_1573 at 1812.571 kb on - strand, within Pf1N1B4_1573 at 1812.571 kb on - strand, within Pf1N1B4_1573 at 1812.571 kb on - strand, within Pf1N1B4_1573 at 1812.769 kb on + strand, within Pf1N1B4_1573 at 1812.769 kb on + strand, within Pf1N1B4_1573 at 1812.769 kb on + strand, within Pf1N1B4_1573 at 1812.770 kb on - strand, within Pf1N1B4_1573 at 1812.869 kb on + strand, within Pf1N1B4_1573 at 1812.870 kb on - strand, within Pf1N1B4_1573 at 1812.870 kb on - strand, within Pf1N1B4_1573 at 1812.914 kb on - strand, within Pf1N1B4_1573 at 1813.218 kb on + strand at 1813.263 kb on - strand at 1813.449 kb on + strand at 1813.450 kb on - strand at 1813.453 kb on + strand at 1813.453 kb on + strand at 1813.454 kb on - strand at 1813.454 kb on - strand at 1813.454 kb on - strand at 1813.454 kb on - strand at 1813.454 kb on - strand at 1813.454 kb on - strand at 1813.498 kb on + strand at 1813.502 kb on + strand at 1813.503 kb on - strand at 1813.503 kb on - strand at 1813.515 kb on + strand at 1813.540 kb on - strand at 1813.540 kb on - strand at 1813.540 kb on - strand at 1813.581 kb on - strand at 1813.581 kb on - strand at 1813.655 kb on + strand, within Pf1N1B4_1574 at 1813.671 kb on - strand, within Pf1N1B4_1574 at 1813.706 kb on + strand, within Pf1N1B4_1574 at 1813.706 kb on + strand, within Pf1N1B4_1574 at 1813.713 kb on + strand, within Pf1N1B4_1574 at 1813.713 kb on + strand, within Pf1N1B4_1574 at 1813.714 kb on - strand at 1813.714 kb on - strand at 1813.714 kb on - strand, within Pf1N1B4_1574 at 1813.714 kb on - strand at 1813.714 kb on - strand, within Pf1N1B4_1574 at 1813.714 kb on - strand, within Pf1N1B4_1574 at 1813.714 kb on - strand, within Pf1N1B4_1574 at 1813.834 kb on - strand, within Pf1N1B4_1574 at 1813.889 kb on + strand, within Pf1N1B4_1574 at 1813.889 kb on + strand, within Pf1N1B4_1574 at 1813.890 kb on - strand, within Pf1N1B4_1574 at 1813.890 kb on - strand, within Pf1N1B4_1574 at 1813.890 kb on - strand, within Pf1N1B4_1574 at 1813.890 kb on - strand, within Pf1N1B4_1574 at 1813.898 kb on + strand, within Pf1N1B4_1574 at 1813.898 kb on + strand, within Pf1N1B4_1574 at 1813.972 kb on - strand at 1813.972 kb on - strand at 1813.972 kb on - strand at 1813.972 kb on - strand at 1814.025 kb on + strand at 1814.025 kb on + strand at 1814.025 kb on + strand at 1814.026 kb on - strand at 1814.108 kb on - strand at 1814.108 kb on - strand at 1814.110 kb on + strand at 1814.110 kb on + strand at 1814.110 kb on + strand at 1814.110 kb on + strand at 1814.110 kb on + strand at 1814.111 kb on - strand at 1814.111 kb on - strand at 1814.111 kb on - strand at 1814.111 kb on - strand at 1814.111 kb on - strand at 1814.111 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 1,810,501 + Pf1N1B4_1572 0.62 -1.9 1,810,508 + Pf1N1B4_1572 0.62 -0.5 1,810,509 - Pf1N1B4_1572 0.62 +1.5 1,810,530 + Pf1N1B4_1572 0.63 +0.5 1,810,794 + Pf1N1B4_1572 0.73 -2.3 1,810,794 + Pf1N1B4_1572 0.73 -0.5 1,810,882 + Pf1N1B4_1572 0.77 -1.8 1,810,883 - Pf1N1B4_1572 0.77 -0.5 1,810,883 - Pf1N1B4_1572 0.77 -1.8 1,810,909 - Pf1N1B4_1572 0.78 -0.1 1,810,909 - Pf1N1B4_1572 0.78 +0.5 1,810,909 - Pf1N1B4_1572 0.78 +0.8 1,811,007 + Pf1N1B4_1572 0.81 -0.1 1,811,007 + Pf1N1B4_1572 0.81 -1.1 1,811,007 + Pf1N1B4_1572 0.81 +0.5 1,811,008 - Pf1N1B4_1572 0.82 -0.7 1,811,055 + Pf1N1B4_1572 0.83 +0.3 1,811,465 + -0.1 1,811,465 + -0.5 1,811,465 + +0.9 1,811,465 + +3.3 1,811,465 + +1.0 1,811,477 - +0.3 1,811,477 - +3.5 1,811,498 - -1.1 1,811,498 - -0.7 1,811,956 + Pf1N1B4_1573 0.27 -1.5 1,811,957 - Pf1N1B4_1573 0.27 -1.5 1,811,957 - Pf1N1B4_1573 0.27 +0.9 1,812,149 + Pf1N1B4_1573 0.38 -0.2 1,812,150 - Pf1N1B4_1573 0.38 -1.9 1,812,271 + Pf1N1B4_1573 0.45 -1.1 1,812,272 - Pf1N1B4_1573 0.45 +1.5 1,812,437 - Pf1N1B4_1573 0.54 -0.9 1,812,570 + Pf1N1B4_1573 0.62 +1.5 1,812,571 - Pf1N1B4_1573 0.62 -0.8 1,812,571 - Pf1N1B4_1573 0.62 -0.1 1,812,571 - Pf1N1B4_1573 0.62 -0.5 1,812,571 - Pf1N1B4_1573 0.62 -0.1 1,812,571 - Pf1N1B4_1573 0.62 -0.8 1,812,769 + Pf1N1B4_1573 0.73 -0.5 1,812,769 + Pf1N1B4_1573 0.73 +0.6 1,812,769 + Pf1N1B4_1573 0.73 +0.5 1,812,770 - Pf1N1B4_1573 0.73 +1.5 1,812,869 + Pf1N1B4_1573 0.78 -1.3 1,812,870 - Pf1N1B4_1573 0.79 -0.1 1,812,870 - Pf1N1B4_1573 0.79 -0.5 1,812,914 - Pf1N1B4_1573 0.81 -0.3 1,813,218 + +2.3 1,813,263 - -0.1 1,813,449 + -0.5 1,813,450 - +0.4 1,813,453 + +1.1 1,813,453 + -0.9 1,813,454 - +1.1 1,813,454 - -0.8 1,813,454 - +0.7 1,813,454 - +2.3 1,813,454 - +0.5 1,813,454 - -0.3 1,813,498 + +1.5 1,813,502 + -0.8 1,813,503 - +0.5 1,813,503 - -0.4 1,813,515 + +0.3 1,813,540 - +1.5 1,813,540 - +0.3 1,813,540 - +1.0 1,813,581 - +0.6 1,813,581 - +0.1 1,813,655 + Pf1N1B4_1574 0.14 -1.7 1,813,671 - Pf1N1B4_1574 0.18 -0.8 1,813,706 + Pf1N1B4_1574 0.27 +0.4 1,813,706 + Pf1N1B4_1574 0.27 -2.1 1,813,713 + Pf1N1B4_1574 0.29 +1.3 1,813,713 + Pf1N1B4_1574 0.29 -0.1 1,813,714 - -0.1 1,813,714 - +0.5 1,813,714 - Pf1N1B4_1574 0.29 +1.2 1,813,714 - +1.5 1,813,714 - Pf1N1B4_1574 0.29 +0.9 1,813,714 - Pf1N1B4_1574 0.29 -0.8 1,813,714 - Pf1N1B4_1574 0.29 +0.2 1,813,834 - Pf1N1B4_1574 0.60 -1.1 1,813,889 + Pf1N1B4_1574 0.75 -0.1 1,813,889 + Pf1N1B4_1574 0.75 -0.1 1,813,890 - Pf1N1B4_1574 0.75 +0.3 1,813,890 - Pf1N1B4_1574 0.75 -0.5 1,813,890 - Pf1N1B4_1574 0.75 -0.4 1,813,890 - Pf1N1B4_1574 0.75 +0.5 1,813,898 + Pf1N1B4_1574 0.77 -1.9 1,813,898 + Pf1N1B4_1574 0.77 -1.8 1,813,972 - -1.1 1,813,972 - +0.4 1,813,972 - -0.2 1,813,972 - +1.5 1,814,025 + -1.7 1,814,025 + +0.2 1,814,025 + +0.9 1,814,026 - -0.1 1,814,108 - +0.5 1,814,108 - -0.5 1,814,110 + -0.8 1,814,110 + +0.3 1,814,110 + +1.2 1,814,110 + +2.4 1,814,110 + +0.5 1,814,111 - +0.6 1,814,111 - -0.8 1,814,111 - -0.2 1,814,111 - +0.9 1,814,111 - +0.9 1,814,111 - +1.5
Or see this region's nucleotide sequence