Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1313 and Pf1N1B4_1314 are separated by 38 nucleotides Pf1N1B4_1314 and Pf1N1B4_1315 are separated by 82 nucleotides Pf1N1B4_1315 and Pf1N1B4_1316 are separated by 151 nucleotides
Pf1N1B4_1313 - tRNA dihydrouridine synthase B (EC 1.-.-.-), at 1,496,027 to 1,497,037
_1313
Pf1N1B4_1314 - DNA-binding protein Fis, at 1,497,076 to 1,497,354
_1314
Pf1N1B4_1315 - IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3), at 1,497,437 to 1,499,044
_1315
Pf1N1B4_1316 - Phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 1,499,196 to 1,500,491
_1316
Position (kb)
1497
1498
1499
1500 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.564 kb on + strand, within Pf1N1B4_1313 at 1496.582 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.753 kb on + strand, within Pf1N1B4_1313 at 1496.843 kb on + strand, within Pf1N1B4_1313 at 1496.843 kb on + strand, within Pf1N1B4_1313 at 1496.868 kb on + strand, within Pf1N1B4_1313 at 1496.868 kb on + strand, within Pf1N1B4_1313 at 1496.891 kb on + strand, within Pf1N1B4_1313 at 1496.891 kb on + strand, within Pf1N1B4_1313 at 1496.891 kb on + strand, within Pf1N1B4_1313 at 1496.891 kb on + strand, within Pf1N1B4_1313 at 1496.969 kb on + strand at 1496.969 kb on + strand at 1496.969 kb on + strand at 1496.969 kb on + strand at 1496.969 kb on + strand at 1496.996 kb on + strand at 1496.996 kb on + strand at 1496.996 kb on + strand at 1496.996 kb on + strand at 1497.056 kb on + strand at 1497.352 kb on + strand at 1497.615 kb on - strand, within Pf1N1B4_1315 at 1497.903 kb on - strand, within Pf1N1B4_1315 at 1497.944 kb on + strand, within Pf1N1B4_1315 at 1497.944 kb on + strand, within Pf1N1B4_1315 at 1497.944 kb on + strand, within Pf1N1B4_1315 at 1497.945 kb on - strand, within Pf1N1B4_1315 at 1497.945 kb on - strand, within Pf1N1B4_1315 at 1497.945 kb on - strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.016 kb on + strand, within Pf1N1B4_1315 at 1498.017 kb on - strand, within Pf1N1B4_1315 at 1498.017 kb on - strand, within Pf1N1B4_1315 at 1498.017 kb on - strand, within Pf1N1B4_1315 at 1498.017 kb on - strand, within Pf1N1B4_1315 at 1498.154 kb on + strand, within Pf1N1B4_1315 at 1498.154 kb on + strand, within Pf1N1B4_1315 at 1498.154 kb on + strand, within Pf1N1B4_1315 at 1498.154 kb on + strand, within Pf1N1B4_1315 at 1498.154 kb on + strand, within Pf1N1B4_1315 at 1498.361 kb on + strand, within Pf1N1B4_1315 at 1498.536 kb on + strand, within Pf1N1B4_1315 at 1498.536 kb on + strand, within Pf1N1B4_1315 at 1498.536 kb on + strand, within Pf1N1B4_1315 at 1498.589 kb on + strand, within Pf1N1B4_1315 at 1498.589 kb on + strand, within Pf1N1B4_1315 at 1498.589 kb on + strand, within Pf1N1B4_1315 at 1498.589 kb on + strand, within Pf1N1B4_1315 at 1498.590 kb on - strand, within Pf1N1B4_1315 at 1498.730 kb on + strand, within Pf1N1B4_1315 at 1498.730 kb on + strand, within Pf1N1B4_1315 at 1498.730 kb on + strand, within Pf1N1B4_1315 at 1498.730 kb on + strand, within Pf1N1B4_1315 at 1498.731 kb on - strand, within Pf1N1B4_1315 at 1498.731 kb on - strand, within Pf1N1B4_1315 at 1498.731 kb on - strand, within Pf1N1B4_1315 at 1498.735 kb on + strand, within Pf1N1B4_1315 at 1498.735 kb on + strand, within Pf1N1B4_1315 at 1498.735 kb on + strand, within Pf1N1B4_1315 at 1498.933 kb on - strand at 1499.173 kb on + strand at 1499.514 kb on - strand, within Pf1N1B4_1316 at 1499.592 kb on + strand, within Pf1N1B4_1316 at 1499.593 kb on - strand, within Pf1N1B4_1316 at 1499.616 kb on - strand, within Pf1N1B4_1316 at 1499.809 kb on + strand, within Pf1N1B4_1316
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 1,496,564 + Pf1N1B4_1313 0.53 +0.8 1,496,564 + Pf1N1B4_1313 0.53 +3.0 1,496,564 + Pf1N1B4_1313 0.53 +0.9 1,496,564 + Pf1N1B4_1313 0.53 -0.2 1,496,564 + Pf1N1B4_1313 0.53 +0.2 1,496,564 + Pf1N1B4_1313 0.53 +0.9 1,496,564 + Pf1N1B4_1313 0.53 +0.1 1,496,564 + Pf1N1B4_1313 0.53 +1.5 1,496,582 + Pf1N1B4_1313 0.55 +0.6 1,496,753 + Pf1N1B4_1313 0.72 +1.4 1,496,753 + Pf1N1B4_1313 0.72 +1.4 1,496,753 + Pf1N1B4_1313 0.72 -0.0 1,496,843 + Pf1N1B4_1313 0.81 +0.7 1,496,843 + Pf1N1B4_1313 0.81 +0.6 1,496,868 + Pf1N1B4_1313 0.83 +0.4 1,496,868 + Pf1N1B4_1313 0.83 +0.7 1,496,891 + Pf1N1B4_1313 0.85 +1.2 1,496,891 + Pf1N1B4_1313 0.85 +0.1 1,496,891 + Pf1N1B4_1313 0.85 -0.4 1,496,891 + Pf1N1B4_1313 0.85 +0.7 1,496,969 + -0.7 1,496,969 + -0.6 1,496,969 + +0.8 1,496,969 + +1.1 1,496,969 + +1.0 1,496,996 + -1.0 1,496,996 + -0.8 1,496,996 + +0.8 1,496,996 + -1.8 1,497,056 + +0.9 1,497,352 + +1.4 1,497,615 - Pf1N1B4_1315 0.11 +0.5 1,497,903 - Pf1N1B4_1315 0.29 -2.4 1,497,944 + Pf1N1B4_1315 0.32 -2.8 1,497,944 + Pf1N1B4_1315 0.32 -2.2 1,497,944 + Pf1N1B4_1315 0.32 -3.5 1,497,945 - Pf1N1B4_1315 0.32 -0.9 1,497,945 - Pf1N1B4_1315 0.32 -1.9 1,497,945 - Pf1N1B4_1315 0.32 +3.0 1,498,016 + Pf1N1B4_1315 0.36 -2.3 1,498,016 + Pf1N1B4_1315 0.36 -1.4 1,498,016 + Pf1N1B4_1315 0.36 -1.6 1,498,016 + Pf1N1B4_1315 0.36 -0.6 1,498,016 + Pf1N1B4_1315 0.36 +1.4 1,498,016 + Pf1N1B4_1315 0.36 -0.9 1,498,016 + Pf1N1B4_1315 0.36 -2.8 1,498,016 + Pf1N1B4_1315 0.36 -0.6 1,498,017 - Pf1N1B4_1315 0.36 -1.8 1,498,017 - Pf1N1B4_1315 0.36 -2.3 1,498,017 - Pf1N1B4_1315 0.36 -0.6 1,498,017 - Pf1N1B4_1315 0.36 -1.6 1,498,154 + Pf1N1B4_1315 0.45 -0.6 1,498,154 + Pf1N1B4_1315 0.45 -3.1 1,498,154 + Pf1N1B4_1315 0.45 -2.6 1,498,154 + Pf1N1B4_1315 0.45 -1.9 1,498,154 + Pf1N1B4_1315 0.45 -1.4 1,498,361 + Pf1N1B4_1315 0.57 -1.5 1,498,536 + Pf1N1B4_1315 0.68 -1.8 1,498,536 + Pf1N1B4_1315 0.68 -2.2 1,498,536 + Pf1N1B4_1315 0.68 -1.4 1,498,589 + Pf1N1B4_1315 0.72 -2.5 1,498,589 + Pf1N1B4_1315 0.72 -1.4 1,498,589 + Pf1N1B4_1315 0.72 -2.7 1,498,589 + Pf1N1B4_1315 0.72 -0.4 1,498,590 - Pf1N1B4_1315 0.72 -0.3 1,498,730 + Pf1N1B4_1315 0.80 -1.2 1,498,730 + Pf1N1B4_1315 0.80 -0.6 1,498,730 + Pf1N1B4_1315 0.80 -2.1 1,498,730 + Pf1N1B4_1315 0.80 -1.2 1,498,731 - Pf1N1B4_1315 0.80 +0.2 1,498,731 - Pf1N1B4_1315 0.80 -0.6 1,498,731 - Pf1N1B4_1315 0.80 -3.1 1,498,735 + Pf1N1B4_1315 0.81 +0.4 1,498,735 + Pf1N1B4_1315 0.81 -2.7 1,498,735 + Pf1N1B4_1315 0.81 -1.1 1,498,933 - +0.4 1,499,173 + -1.6 1,499,514 - Pf1N1B4_1316 0.25 +1.4 1,499,592 + Pf1N1B4_1316 0.31 +0.4 1,499,593 - Pf1N1B4_1316 0.31 +0.4 1,499,616 - Pf1N1B4_1316 0.32 -0.9 1,499,809 + Pf1N1B4_1316 0.47 -0.9
Or see this region's nucleotide sequence