Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_124 and Pf1N1B4_125 are separated by 189 nucleotides Pf1N1B4_125 and Pf1N1B4_126 are separated by 127 nucleotides Pf1N1B4_126 and Pf1N1B4_127 are separated by 105 nucleotides
Pf1N1B4_124 - Mechanosensitive ion channel, at 135,578 to 137,023
_124
Pf1N1B4_125 - Nicotinamidase family protein YcaC, at 137,213 to 137,839
_125
Pf1N1B4_126 - Pirin, at 137,967 to 138,833
_126
Pf1N1B4_127 - FIG032225: Transcriptional regulator, LysR family, at 138,939 to 139,847
_127
Position (kb)
137
138
139 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 137.040 kb on + strand at 137.041 kb on - strand at 137.043 kb on + strand at 137.044 kb on - strand at 137.044 kb on - strand at 137.060 kb on + strand at 137.061 kb on - strand at 137.061 kb on - strand at 137.061 kb on - strand at 137.128 kb on - strand at 137.189 kb on + strand at 137.189 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.214 kb on + strand at 137.215 kb on - strand at 137.238 kb on + strand at 137.239 kb on - strand at 137.239 kb on - strand at 137.695 kb on - strand, within Pf1N1B4_125 at 137.834 kb on - strand at 137.834 kb on - strand at 137.924 kb on + strand at 137.955 kb on - strand at 137.955 kb on - strand at 138.289 kb on - strand, within Pf1N1B4_126 at 138.616 kb on + strand, within Pf1N1B4_126 at 138.616 kb on + strand, within Pf1N1B4_126 at 138.616 kb on + strand, within Pf1N1B4_126 at 138.811 kb on + strand at 138.926 kb on + strand at 138.964 kb on - strand at 138.966 kb on + strand at 138.967 kb on - strand at 139.134 kb on + strand, within Pf1N1B4_127 at 139.134 kb on + strand, within Pf1N1B4_127 at 139.281 kb on + strand, within Pf1N1B4_127 at 139.281 kb on + strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.282 kb on - strand, within Pf1N1B4_127 at 139.293 kb on + strand, within Pf1N1B4_127 at 139.293 kb on + strand, within Pf1N1B4_127 at 139.294 kb on - strand, within Pf1N1B4_127 at 139.498 kb on + strand, within Pf1N1B4_127 at 139.542 kb on + strand, within Pf1N1B4_127
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 137,040 + -0.2 137,041 - +0.0 137,043 + -1.2 137,044 - +0.3 137,044 - -0.1 137,060 + +0.8 137,061 - +0.4 137,061 - +1.2 137,061 - -1.6 137,128 - -1.2 137,189 + +0.0 137,189 + -1.4 137,197 + +0.8 137,197 + -0.2 137,197 + +0.2 137,197 + -1.2 137,197 + -2.0 137,198 - +0.5 137,198 - -0.2 137,198 - +1.4 137,198 - +0.8 137,198 - -0.2 137,214 + -1.6 137,215 - +0.4 137,238 + +0.8 137,239 - -0.2 137,239 - -1.6 137,695 - Pf1N1B4_125 0.77 -0.6 137,834 - +0.8 137,834 - +0.8 137,924 + -0.6 137,955 - -0.6 137,955 - +0.4 138,289 - Pf1N1B4_126 0.37 -2.2 138,616 + Pf1N1B4_126 0.75 -1.8 138,616 + Pf1N1B4_126 0.75 -0.2 138,616 + Pf1N1B4_126 0.75 -3.3 138,811 + -1.6 138,926 + +2.1 138,964 - -1.6 138,966 + -1.8 138,967 - -1.0 139,134 + Pf1N1B4_127 0.21 -1.0 139,134 + Pf1N1B4_127 0.21 -0.6 139,281 + Pf1N1B4_127 0.38 +1.1 139,281 + Pf1N1B4_127 0.38 +1.2 139,282 - Pf1N1B4_127 0.38 +1.4 139,282 - Pf1N1B4_127 0.38 +0.8 139,282 - Pf1N1B4_127 0.38 -0.8 139,282 - Pf1N1B4_127 0.38 -0.0 139,293 + Pf1N1B4_127 0.39 +2.4 139,293 + Pf1N1B4_127 0.39 +1.2 139,294 - Pf1N1B4_127 0.39 +1.4 139,498 + Pf1N1B4_127 0.61 +3.4 139,542 + Pf1N1B4_127 0.66 +2.1
Or see this region's nucleotide sequence