Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1209 and Pf1N1B4_1210 overlap by 11 nucleotides Pf1N1B4_1210 and Pf1N1B4_1211 are separated by 234 nucleotides Pf1N1B4_1211 and Pf1N1B4_1212 are separated by 418 nucleotides Pf1N1B4_1212 and Pf1N1B4_1213 are separated by 84 nucleotides
Pf1N1B4_1209 - FIG00959330: hypothetical protein, at 1,372,393 to 1,372,737
_1209
Pf1N1B4_1210 - membrane protein, putative, at 1,372,727 to 1,373,491
_1210
Pf1N1B4_1211 - Hydrogen peroxide-inducible genes activator, at 1,373,726 to 1,374,658
_1211
Pf1N1B4_1212 - Predicted flavin reductase RutF in novel pyrimidine catabolism pathway, at 1,375,077 to 1,375,379
_1212
Pf1N1B4_1213 - Transcriptional regulator, at 1,375,464 to 1,375,739
_1213
Position (kb)
1373
1374
1375 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.760 kb on - strand at 1372.778 kb on + strand at 1372.779 kb on - strand at 1372.998 kb on + strand, within Pf1N1B4_1210 at 1372.998 kb on + strand, within Pf1N1B4_1210 at 1372.999 kb on - strand, within Pf1N1B4_1210 at 1372.999 kb on - strand, within Pf1N1B4_1210 at 1372.999 kb on - strand, within Pf1N1B4_1210 at 1373.133 kb on + strand, within Pf1N1B4_1210 at 1373.133 kb on + strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.135 kb on + strand, within Pf1N1B4_1210 at 1373.136 kb on - strand, within Pf1N1B4_1210 at 1373.441 kb on + strand at 1373.469 kb on + strand at 1373.470 kb on - strand at 1373.485 kb on + strand at 1373.486 kb on - strand at 1373.486 kb on - strand at 1373.498 kb on - strand at 1373.534 kb on + strand at 1373.534 kb on + strand at 1373.534 kb on + strand at 1373.535 kb on - strand at 1373.536 kb on + strand at 1373.536 kb on + strand at 1373.536 kb on + strand at 1373.756 kb on + strand at 1373.757 kb on - strand at 1373.757 kb on - strand at 1374.026 kb on - strand, within Pf1N1B4_1211 at 1374.026 kb on - strand, within Pf1N1B4_1211 at 1374.045 kb on - strand, within Pf1N1B4_1211 at 1374.045 kb on - strand, within Pf1N1B4_1211 at 1374.273 kb on + strand, within Pf1N1B4_1211 at 1374.273 kb on + strand, within Pf1N1B4_1211 at 1374.302 kb on + strand, within Pf1N1B4_1211 at 1374.302 kb on + strand, within Pf1N1B4_1211 at 1374.303 kb on - strand at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.338 kb on + strand, within Pf1N1B4_1211 at 1374.338 kb on + strand, within Pf1N1B4_1211 at 1374.651 kb on + strand at 1374.651 kb on + strand at 1374.651 kb on + strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.724 kb on + strand at 1374.724 kb on + strand at 1374.725 kb on - strand at 1374.731 kb on - strand at 1374.737 kb on + strand at 1374.737 kb on + strand at 1374.738 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.766 kb on + strand at 1374.767 kb on - strand at 1374.768 kb on + strand at 1374.847 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.872 kb on + strand at 1374.872 kb on + strand at 1374.873 kb on - strand at 1374.873 kb on - strand at 1374.897 kb on - strand at 1375.320 kb on + strand, within Pf1N1B4_1212 at 1375.321 kb on - strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.469 kb on - strand at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.495 kb on + strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.514 kb on + strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.547 kb on + strand, within Pf1N1B4_1213
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 1,372,759 + -0.2 1,372,759 + -0.5 1,372,759 + -0.1 1,372,759 + +0.5 1,372,759 + -1.0 1,372,759 + +0.6 1,372,760 - -2.0 1,372,778 + +1.0 1,372,779 - +0.3 1,372,998 + Pf1N1B4_1210 0.35 +0.2 1,372,998 + Pf1N1B4_1210 0.35 -0.1 1,372,999 - Pf1N1B4_1210 0.36 -0.1 1,372,999 - Pf1N1B4_1210 0.36 +1.8 1,372,999 - Pf1N1B4_1210 0.36 -0.2 1,373,133 + Pf1N1B4_1210 0.53 -2.0 1,373,133 + Pf1N1B4_1210 0.53 +0.3 1,373,134 - Pf1N1B4_1210 0.53 -0.2 1,373,134 - Pf1N1B4_1210 0.53 -1.0 1,373,134 - Pf1N1B4_1210 0.53 -2.4 1,373,134 - Pf1N1B4_1210 0.53 +0.8 1,373,134 - Pf1N1B4_1210 0.53 -0.7 1,373,134 - Pf1N1B4_1210 0.53 +0.5 1,373,134 - -0.7 1,373,134 - Pf1N1B4_1210 0.53 -1.7 1,373,134 - Pf1N1B4_1210 0.53 -0.2 1,373,135 + Pf1N1B4_1210 0.53 +1.1 1,373,136 - Pf1N1B4_1210 0.53 -0.2 1,373,441 + -0.2 1,373,469 + -0.9 1,373,470 - -0.4 1,373,485 + -0.3 1,373,486 - -0.1 1,373,486 - +0.2 1,373,498 - +0.0 1,373,534 + -0.2 1,373,534 + -1.1 1,373,534 + +0.0 1,373,535 - -0.4 1,373,536 + -2.0 1,373,536 + -0.9 1,373,536 + -1.0 1,373,756 + -1.5 1,373,757 - +0.8 1,373,757 - -0.5 1,374,026 - Pf1N1B4_1211 0.32 +0.8 1,374,026 - Pf1N1B4_1211 0.32 -0.1 1,374,045 - Pf1N1B4_1211 0.34 +1.0 1,374,045 - Pf1N1B4_1211 0.34 -0.2 1,374,273 + Pf1N1B4_1211 0.59 -2.5 1,374,273 + Pf1N1B4_1211 0.59 -0.7 1,374,302 + Pf1N1B4_1211 0.62 -1.2 1,374,302 + Pf1N1B4_1211 0.62 -2.5 1,374,303 - +1.3 1,374,303 - Pf1N1B4_1211 0.62 -1.7 1,374,303 - Pf1N1B4_1211 0.62 -1.2 1,374,303 - Pf1N1B4_1211 0.62 -2.5 1,374,303 - Pf1N1B4_1211 0.62 -0.5 1,374,303 - Pf1N1B4_1211 0.62 -1.1 1,374,303 - Pf1N1B4_1211 0.62 +0.9 1,374,303 - Pf1N1B4_1211 0.62 +1.0 1,374,303 - +1.3 1,374,303 - Pf1N1B4_1211 0.62 -1.0 1,374,338 + Pf1N1B4_1211 0.66 -2.7 1,374,338 + Pf1N1B4_1211 0.66 -1.0 1,374,651 + -1.0 1,374,651 + +1.3 1,374,651 + +1.3 1,374,652 - -0.1 1,374,652 - -0.7 1,374,652 - +1.3 1,374,652 - +1.3 1,374,724 + -1.2 1,374,724 + -0.7 1,374,725 - +0.9 1,374,731 - +1.3 1,374,737 + -2.0 1,374,737 + -0.7 1,374,738 - +1.1 1,374,760 - -0.4 1,374,760 - +0.3 1,374,760 - -1.0 1,374,760 - +1.0 1,374,760 - -0.7 1,374,763 + -2.0 1,374,763 + -0.1 1,374,763 + -2.1 1,374,763 + +0.0 1,374,764 - -0.2 1,374,764 - +2.3 1,374,764 - +0.2 1,374,764 - -0.7 1,374,766 + -0.5 1,374,767 - -1.5 1,374,768 + +0.0 1,374,847 + +0.5 1,374,852 + -0.2 1,374,852 + -1.2 1,374,852 + +0.1 1,374,852 + +1.9 1,374,852 + -1.8 1,374,853 - -0.2 1,374,853 - +0.0 1,374,853 - -0.1 1,374,853 - -1.7 1,374,853 - +1.0 1,374,853 - -2.1 1,374,853 - -0.0 1,374,853 - +1.6 1,374,853 - +1.3 1,374,872 + +0.3 1,374,872 + -0.0 1,374,873 - +1.1 1,374,873 - +0.0 1,374,897 - +0.2 1,375,320 + Pf1N1B4_1212 0.80 +1.3 1,375,321 - Pf1N1B4_1212 0.81 +0.9 1,375,335 + Pf1N1B4_1212 0.85 -0.1 1,375,335 + Pf1N1B4_1212 0.85 +0.2 1,375,335 + Pf1N1B4_1212 0.85 +0.3 1,375,335 + Pf1N1B4_1212 0.85 +0.1 1,375,335 + Pf1N1B4_1212 0.85 +0.2 1,375,335 + Pf1N1B4_1212 0.85 +0.2 1,375,336 - Pf1N1B4_1212 0.85 -0.7 1,375,336 - Pf1N1B4_1212 0.85 +1.8 1,375,469 - +0.3 1,375,494 - Pf1N1B4_1213 0.11 -0.2 1,375,494 - Pf1N1B4_1213 0.11 -0.2 1,375,495 + Pf1N1B4_1213 0.11 -1.0 1,375,496 - Pf1N1B4_1213 0.12 -1.2 1,375,496 - Pf1N1B4_1213 0.12 +0.2 1,375,502 + Pf1N1B4_1213 0.14 +0.8 1,375,502 + Pf1N1B4_1213 0.14 +0.3 1,375,503 - Pf1N1B4_1213 0.14 +0.3 1,375,503 - Pf1N1B4_1213 0.14 -1.0 1,375,503 - Pf1N1B4_1213 0.14 -0.6 1,375,503 - Pf1N1B4_1213 0.14 -1.0 1,375,503 - Pf1N1B4_1213 0.14 +0.1 1,375,514 + Pf1N1B4_1213 0.18 -2.0 1,375,515 - Pf1N1B4_1213 0.18 +1.2 1,375,515 - Pf1N1B4_1213 0.18 +0.9 1,375,515 - Pf1N1B4_1213 0.18 +0.6 1,375,515 - Pf1N1B4_1213 0.18 -0.2 1,375,547 + Pf1N1B4_1213 0.30 +0.9
Or see this region's nucleotide sequence