Experiment: Sisomicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_104 and Pf1N1B4_105 overlap by 4 nucleotides Pf1N1B4_105 and Pf1N1B4_106 are separated by 3 nucleotides Pf1N1B4_106 and Pf1N1B4_107 are separated by 38 nucleotides Pf1N1B4_107 and Pf1N1B4_108 are separated by 243 nucleotides
Pf1N1B4_104 - Sensor protein PhoQ (EC 2.7.13.3), at 110,365 to 111,678
_104
Pf1N1B4_105 - Two-component system response regulator QseB, at 111,675 to 112,343
_105
Pf1N1B4_106 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 112,347 to 112,628
_106
Pf1N1B4_107 - FIG00857858: hypothetical protein, at 112,667 to 112,975
_107
Pf1N1B4_108 - FIG00954456: hypothetical protein, at 113,219 to 114,298
_108
Position (kb)
112
113 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 111.996 kb on + strand, within Pf1N1B4_105 at 112.232 kb on - strand, within Pf1N1B4_105 at 112.364 kb on + strand at 112.364 kb on + strand at 112.368 kb on + strand at 112.368 kb on + strand at 112.368 kb on + strand at 112.369 kb on - strand at 112.395 kb on + strand, within Pf1N1B4_106 at 112.409 kb on + strand, within Pf1N1B4_106 at 112.438 kb on + strand, within Pf1N1B4_106 at 112.438 kb on + strand at 112.438 kb on + strand, within Pf1N1B4_106 at 112.444 kb on + strand, within Pf1N1B4_106 at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.761 kb on + strand, within Pf1N1B4_107 at 112.762 kb on - strand, within Pf1N1B4_107 at 112.767 kb on + strand, within Pf1N1B4_107 at 112.767 kb on + strand, within Pf1N1B4_107 at 112.767 kb on + strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.777 kb on - strand at 112.864 kb on + strand, within Pf1N1B4_107 at 112.865 kb on - strand, within Pf1N1B4_107 at 112.988 kb on + strand at 113.006 kb on + strand at 113.006 kb on + strand at 113.007 kb on - strand at 113.007 kb on - strand at 113.016 kb on - strand at 113.016 kb on - strand at 113.016 kb on - strand at 113.020 kb on + strand at 113.020 kb on + strand at 113.035 kb on - strand at 113.087 kb on - strand at 113.126 kb on + strand at 113.127 kb on - strand at 113.128 kb on + strand at 113.154 kb on + strand at 113.154 kb on + strand at 113.155 kb on - strand at 113.510 kb on + strand, within Pf1N1B4_108 at 113.510 kb on + strand, within Pf1N1B4_108 at 113.961 kb on - strand, within Pf1N1B4_108
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.001 mg/ml remove 111,996 + Pf1N1B4_105 0.48 +1.0 112,232 - Pf1N1B4_105 0.83 -0.6 112,364 + +0.8 112,364 + +1.0 112,368 + +1.3 112,368 + +0.6 112,368 + -0.7 112,369 - -1.2 112,395 + Pf1N1B4_106 0.17 +1.1 112,409 + Pf1N1B4_106 0.22 +2.3 112,438 + Pf1N1B4_106 0.32 +1.3 112,438 + +0.8 112,438 + Pf1N1B4_106 0.32 +0.7 112,444 + Pf1N1B4_106 0.34 +1.5 112,668 + +0.6 112,668 + +0.8 112,668 + +0.8 112,668 + -0.0 112,668 + +1.5 112,761 + Pf1N1B4_107 0.30 +2.1 112,762 - Pf1N1B4_107 0.31 -0.8 112,767 + Pf1N1B4_107 0.32 -1.6 112,767 + Pf1N1B4_107 0.32 +0.8 112,767 + Pf1N1B4_107 0.32 -0.5 112,768 - Pf1N1B4_107 0.33 +0.4 112,768 - Pf1N1B4_107 0.33 +0.2 112,768 - Pf1N1B4_107 0.33 +0.8 112,768 - Pf1N1B4_107 0.33 +1.6 112,777 - -0.8 112,864 + Pf1N1B4_107 0.64 +0.8 112,865 - Pf1N1B4_107 0.64 +1.2 112,988 + -0.2 113,006 + -0.8 113,006 + +0.8 113,007 - +1.8 113,007 - +1.8 113,016 - -0.1 113,016 - +1.4 113,016 - +1.2 113,020 + +0.4 113,020 + -1.2 113,035 - +0.8 113,087 - +1.2 113,126 + -0.2 113,127 - +0.0 113,128 + -0.5 113,154 + +0.7 113,154 + +0.4 113,155 - +0.8 113,510 + Pf1N1B4_108 0.27 -2.7 113,510 + Pf1N1B4_108 0.27 -0.8 113,961 - Pf1N1B4_108 0.69 -0.2
Or see this region's nucleotide sequence