Experiment: PYE with Chloride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00224 and CCNA_00225 are separated by 330 nucleotides CCNA_00225 and CCNA_00226 are separated by 100 nucleotides CCNA_00226 and CCNA_00227 are separated by 57 nucleotides CCNA_00227 and CCNA_00228 overlap by 1 nucleotides
CCNA_00224: CCNA_00224 - TonB-dependent receptor protein, at 240,648 to 242,693
_00224
CCNA_00225: CCNA_00225 - hypothetical protein, at 243,024 to 243,056
_00225
CCNA_00226: CCNA_00226 - hypothetical protein, at 243,157 to 243,432
_00226
CCNA_00227: CCNA_00227 - methyltransferase, at 243,490 to 244,056
_00227
CCNA_00228: CCNA_00228 - putative membrane spanning protein, at 244,056 to 244,670
_00228
Position (kb) 243
244 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 242.164 kb on + strand, within CCNA_00224 at 242.164 kb on + strand, within CCNA_00224 at 242.164 kb on - strand, within CCNA_00224 at 242.170 kb on - strand, within CCNA_00224 at 242.172 kb on - strand, within CCNA_00224 at 242.172 kb on - strand, within CCNA_00224 at 242.188 kb on + strand, within CCNA_00224 at 242.196 kb on - strand, within CCNA_00224 at 242.209 kb on + strand, within CCNA_00224 at 242.224 kb on - strand, within CCNA_00224 at 242.224 kb on - strand, within CCNA_00224 at 242.250 kb on + strand, within CCNA_00224 at 242.264 kb on + strand, within CCNA_00224 at 242.300 kb on + strand, within CCNA_00224 at 242.305 kb on + strand, within CCNA_00224 at 242.338 kb on + strand, within CCNA_00224 at 242.343 kb on + strand, within CCNA_00224 at 242.373 kb on - strand, within CCNA_00224 at 242.428 kb on - strand, within CCNA_00224 at 242.441 kb on - strand, within CCNA_00224 at 242.441 kb on - strand, within CCNA_00224 at 242.481 kb on + strand, within CCNA_00224 at 242.489 kb on - strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.565 kb on - strand at 242.573 kb on - strand at 242.587 kb on + strand at 242.587 kb on + strand at 242.595 kb on - strand at 242.600 kb on - strand at 242.643 kb on + strand at 242.656 kb on - strand at 242.675 kb on - strand at 242.683 kb on - strand at 242.704 kb on + strand at 242.715 kb on + strand at 242.716 kb on - strand at 242.718 kb on - strand at 242.723 kb on - strand at 242.723 kb on - strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.904 kb on + strand at 242.932 kb on + strand at 242.987 kb on + strand at 242.995 kb on - strand at 243.010 kb on + strand at 243.018 kb on - strand at 243.020 kb on + strand at 243.028 kb on - strand, within CCNA_00225 at 243.045 kb on + strand, within CCNA_00225 at 243.050 kb on + strand, within CCNA_00225 at 243.050 kb on + strand, within CCNA_00225 at 243.068 kb on - strand at 243.083 kb on + strand at 243.091 kb on - strand at 243.154 kb on + strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.164 kb on + strand at 243.164 kb on + strand at 243.164 kb on + strand at 243.198 kb on + strand, within CCNA_00226 at 243.198 kb on + strand, within CCNA_00226 at 243.202 kb on - strand, within CCNA_00226 at 243.205 kb on + strand, within CCNA_00226 at 243.206 kb on - strand, within CCNA_00226 at 243.213 kb on + strand, within CCNA_00226 at 243.246 kb on - strand, within CCNA_00226 at 243.259 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.276 kb on - strand, within CCNA_00226 at 243.283 kb on - strand, within CCNA_00226 at 243.304 kb on + strand, within CCNA_00226 at 243.400 kb on + strand, within CCNA_00226 at 243.424 kb on - strand at 243.431 kb on - strand at 243.434 kb on + strand at 243.449 kb on + strand at 243.449 kb on + strand at 243.449 kb on + strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.462 kb on + strand at 243.470 kb on - strand at 243.479 kb on - strand at 243.489 kb on - strand at 243.489 kb on - strand at 243.495 kb on - strand at 243.528 kb on - strand at 243.539 kb on + strand at 243.547 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.598 kb on - strand, within CCNA_00227 at 243.620 kb on + strand, within CCNA_00227 at 243.647 kb on - strand, within CCNA_00227 at 243.647 kb on - strand, within CCNA_00227 at 243.673 kb on - strand, within CCNA_00227 at 243.708 kb on + strand, within CCNA_00227 at 243.729 kb on + strand, within CCNA_00227 at 243.729 kb on + strand, within CCNA_00227 at 243.729 kb on + strand, within CCNA_00227 at 243.737 kb on - strand, within CCNA_00227 at 243.751 kb on - strand, within CCNA_00227 at 243.756 kb on - strand, within CCNA_00227 at 243.756 kb on - strand, within CCNA_00227 at 243.767 kb on + strand, within CCNA_00227 at 243.783 kb on - strand, within CCNA_00227 at 243.783 kb on - strand, within CCNA_00227 at 243.844 kb on + strand, within CCNA_00227 at 243.848 kb on - strand, within CCNA_00227 at 243.869 kb on + strand, within CCNA_00227 at 243.932 kb on - strand, within CCNA_00227 at 243.932 kb on - strand, within CCNA_00227 at 243.939 kb on - strand, within CCNA_00227 at 243.955 kb on + strand, within CCNA_00227 at 243.956 kb on - strand, within CCNA_00227 at 243.977 kb on - strand, within CCNA_00227 at 244.016 kb on + strand at 244.016 kb on + strand at 244.022 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Chloride 100 mM remove 242,164 + CCNA_00224 0.74 +0.6 242,164 + CCNA_00224 0.74 +1.3 242,164 - CCNA_00224 0.74 -0.8 242,170 - CCNA_00224 0.74 +0.3 242,172 - CCNA_00224 0.74 +1.1 242,172 - CCNA_00224 0.74 +0.3 242,188 + CCNA_00224 0.75 +1.7 242,196 - CCNA_00224 0.76 -0.3 242,209 + CCNA_00224 0.76 +0.1 242,224 - CCNA_00224 0.77 -0.5 242,224 - CCNA_00224 0.77 -1.3 242,250 + CCNA_00224 0.78 -0.0 242,264 + CCNA_00224 0.79 +0.8 242,300 + CCNA_00224 0.81 -0.8 242,305 + CCNA_00224 0.81 +0.6 242,338 + CCNA_00224 0.83 -1.1 242,343 + CCNA_00224 0.83 +0.2 242,373 - CCNA_00224 0.84 +0.1 242,428 - CCNA_00224 0.87 +0.1 242,441 - CCNA_00224 0.88 +1.1 242,441 - CCNA_00224 0.88 -0.4 242,481 + CCNA_00224 0.90 -1.3 242,489 - -0.0 242,527 + -0.4 242,527 + +0.1 242,527 + +0.4 242,527 + -0.0 242,527 + +0.0 242,527 + +0.7 242,527 + -1.0 242,527 + -0.0 242,527 + +0.5 242,527 + -0.5 242,527 + +0.4 242,535 - -1.0 242,535 - -0.1 242,535 - +1.4 242,535 - +0.2 242,535 - +0.7 242,565 - +0.4 242,573 - +0.0 242,587 + +1.1 242,587 + -0.7 242,595 - +0.7 242,600 - +0.0 242,643 + +0.5 242,656 - +0.1 242,675 - +0.3 242,683 - -0.2 242,704 + +1.1 242,715 + -0.5 242,716 - +0.7 242,718 - -0.7 242,723 - -0.7 242,723 - -3.1 242,892 + +0.1 242,892 + -0.9 242,892 + +0.6 242,892 + +1.1 242,892 + +0.2 242,892 + -0.0 242,900 - -1.2 242,900 - +0.7 242,900 - -0.8 242,900 - -0.6 242,900 - +0.3 242,900 - +0.5 242,900 - +1.7 242,900 - +0.1 242,900 - -1.9 242,904 + -1.0 242,932 + -2.4 242,987 + -0.3 242,995 - +1.7 243,010 + -0.7 243,018 - -0.9 243,020 + +1.3 243,028 - CCNA_00225 0.12 -2.9 243,045 + CCNA_00225 0.64 -0.8 243,050 + CCNA_00225 0.79 -1.5 243,050 + CCNA_00225 0.79 -1.3 243,068 - -0.5 243,083 + -1.4 243,091 - -0.6 243,154 + -0.4 243,162 - -1.3 243,162 - -1.4 243,162 - -0.0 243,162 - +0.2 243,164 + -0.4 243,164 + +1.1 243,164 + -1.5 243,198 + CCNA_00226 0.15 +0.3 243,198 + CCNA_00226 0.15 -0.8 243,202 - CCNA_00226 0.16 +0.5 243,205 + CCNA_00226 0.17 -0.6 243,206 - CCNA_00226 0.18 -1.5 243,213 + CCNA_00226 0.20 -0.7 243,246 - CCNA_00226 0.32 -1.8 243,259 + CCNA_00226 0.37 -0.8 243,275 + CCNA_00226 0.43 +0.0 243,275 + CCNA_00226 0.43 -0.1 243,276 - CCNA_00226 0.43 -1.9 243,283 - CCNA_00226 0.46 -0.4 243,304 + CCNA_00226 0.53 +0.0 243,400 + CCNA_00226 0.88 +0.5 243,424 - -1.8 243,431 - -1.1 243,434 + +0.4 243,449 + +0.7 243,449 + -0.5 243,449 + -0.3 243,457 - +0.0 243,457 - +1.2 243,457 - +0.0 243,457 - +1.3 243,462 + +0.0 243,470 - +0.1 243,479 - +0.2 243,489 - +0.9 243,489 - -0.4 243,495 - -0.0 243,528 - -1.4 243,539 + -1.2 243,547 - CCNA_00227 0.10 -1.3 243,557 - CCNA_00227 0.12 +0.1 243,557 - CCNA_00227 0.12 +0.3 243,557 - CCNA_00227 0.12 +0.2 243,598 - CCNA_00227 0.19 -0.8 243,620 + CCNA_00227 0.23 -0.6 243,647 - CCNA_00227 0.28 -0.6 243,647 - CCNA_00227 0.28 -0.0 243,673 - CCNA_00227 0.32 -2.2 243,708 + CCNA_00227 0.38 +0.1 243,729 + CCNA_00227 0.42 +0.5 243,729 + CCNA_00227 0.42 -1.8 243,729 + CCNA_00227 0.42 -1.1 243,737 - CCNA_00227 0.44 +1.4 243,751 - CCNA_00227 0.46 -0.2 243,756 - CCNA_00227 0.47 -2.2 243,756 - CCNA_00227 0.47 -0.4 243,767 + CCNA_00227 0.49 -1.4 243,783 - CCNA_00227 0.52 -0.7 243,783 - CCNA_00227 0.52 +1.1 243,844 + CCNA_00227 0.62 +0.1 243,848 - CCNA_00227 0.63 +0.5 243,869 + CCNA_00227 0.67 -2.1 243,932 - CCNA_00227 0.78 -0.1 243,932 - CCNA_00227 0.78 +0.9 243,939 - CCNA_00227 0.79 -0.6 243,955 + CCNA_00227 0.82 +0.4 243,956 - CCNA_00227 0.82 -1.1 243,977 - CCNA_00227 0.86 +1.0 244,016 + -1.0 244,016 + -1.0 244,022 + +0.9
Or see this region's nucleotide sequence