Experiment: PYE with Chloride 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00173 and CCNA_00174 overlap by 14 nucleotides CCNA_00174 and CCNA_00175 are separated by 11 nucleotides CCNA_00175 and CCNA_00176 overlap by 86 nucleotides CCNA_00176 and CCNA_00177 overlap by 32 nucleotides
CCNA_00173: CCNA_00173 - type II secretion pathway protein E, at 188,347 to 189,822
_00173
CCNA_00174: CCNA_00174 - type II secretion pathway protein F, at 189,809 to 191,020
_00174
CCNA_00175: CCNA_00175 - type II secretion pathway protein G, at 191,032 to 191,484
_00175
CCNA_00176: CCNA_00176 - type II secretion pathway protein H, at 191,399 to 191,956
_00176
CCNA_00177: CCNA_00177 - type II secretion pathway protein I, at 191,925 to 192,308
_00177
Position (kb) 189
190
191
192 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 188.838 kb on + strand, within CCNA_00173 at 188.838 kb on + strand, within CCNA_00173 at 188.844 kb on + strand, within CCNA_00173 at 188.846 kb on - strand, within CCNA_00173 at 188.846 kb on - strand, within CCNA_00173 at 188.846 kb on - strand, within CCNA_00173 at 188.846 kb on - strand, within CCNA_00173 at 188.846 kb on - strand, within CCNA_00173 at 188.846 kb on - strand, within CCNA_00173 at 188.852 kb on - strand at 188.925 kb on + strand, within CCNA_00173 at 188.929 kb on - strand, within CCNA_00173 at 188.935 kb on + strand, within CCNA_00173 at 188.971 kb on - strand, within CCNA_00173 at 188.996 kb on + strand, within CCNA_00173 at 189.001 kb on + strand, within CCNA_00173 at 189.009 kb on - strand, within CCNA_00173 at 189.011 kb on + strand, within CCNA_00173 at 189.011 kb on - strand, within CCNA_00173 at 189.016 kb on - strand, within CCNA_00173 at 189.018 kb on + strand, within CCNA_00173 at 189.020 kb on - strand, within CCNA_00173 at 189.025 kb on - strand, within CCNA_00173 at 189.051 kb on + strand, within CCNA_00173 at 189.187 kb on + strand, within CCNA_00173 at 189.199 kb on + strand, within CCNA_00173 at 189.211 kb on + strand, within CCNA_00173 at 189.216 kb on + strand, within CCNA_00173 at 189.219 kb on - strand, within CCNA_00173 at 189.222 kb on + strand, within CCNA_00173 at 189.222 kb on + strand, within CCNA_00173 at 189.238 kb on + strand, within CCNA_00173 at 189.263 kb on + strand, within CCNA_00173 at 189.281 kb on + strand, within CCNA_00173 at 189.325 kb on + strand, within CCNA_00173 at 189.337 kb on + strand, within CCNA_00173 at 189.346 kb on + strand, within CCNA_00173 at 189.403 kb on + strand, within CCNA_00173 at 189.406 kb on - strand, within CCNA_00173 at 189.444 kb on + strand, within CCNA_00173 at 189.444 kb on + strand, within CCNA_00173 at 189.452 kb on - strand, within CCNA_00173 at 189.454 kb on - strand, within CCNA_00173 at 189.483 kb on - strand, within CCNA_00173 at 189.538 kb on + strand, within CCNA_00173 at 189.552 kb on + strand, within CCNA_00173 at 189.560 kb on - strand, within CCNA_00173 at 189.603 kb on + strand, within CCNA_00173 at 189.632 kb on + strand, within CCNA_00173 at 189.637 kb on + strand, within CCNA_00173 at 189.638 kb on - strand, within CCNA_00173 at 189.640 kb on - strand, within CCNA_00173 at 189.655 kb on + strand, within CCNA_00173 at 189.663 kb on - strand, within CCNA_00173 at 189.688 kb on + strand at 189.718 kb on - strand at 189.730 kb on - strand at 189.748 kb on + strand at 189.751 kb on - strand at 189.756 kb on - strand at 189.766 kb on - strand at 189.777 kb on - strand at 189.815 kb on + strand at 189.816 kb on + strand at 189.816 kb on + strand at 189.829 kb on + strand at 189.873 kb on - strand at 189.873 kb on - strand at 189.894 kb on - strand at 189.974 kb on + strand, within CCNA_00174 at 189.994 kb on - strand, within CCNA_00174 at 189.994 kb on - strand, within CCNA_00174 at 190.050 kb on + strand, within CCNA_00174 at 190.052 kb on + strand, within CCNA_00174 at 190.054 kb on + strand, within CCNA_00174 at 190.056 kb on + strand, within CCNA_00174 at 190.066 kb on + strand, within CCNA_00174 at 190.100 kb on - strand, within CCNA_00174 at 190.120 kb on + strand, within CCNA_00174 at 190.121 kb on - strand, within CCNA_00174 at 190.133 kb on + strand, within CCNA_00174 at 190.143 kb on - strand, within CCNA_00174 at 190.143 kb on - strand, within CCNA_00174 at 190.143 kb on - strand, within CCNA_00174 at 190.161 kb on - strand, within CCNA_00174 at 190.198 kb on - strand, within CCNA_00174 at 190.203 kb on - strand, within CCNA_00174 at 190.237 kb on + strand, within CCNA_00174 at 190.280 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.307 kb on + strand, within CCNA_00174 at 190.312 kb on + strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.315 kb on - strand, within CCNA_00174 at 190.315 kb on - strand at 190.315 kb on - strand, within CCNA_00174 at 190.320 kb on - strand, within CCNA_00174 at 190.378 kb on + strand, within CCNA_00174 at 190.385 kb on - strand, within CCNA_00174 at 190.409 kb on + strand, within CCNA_00174 at 190.417 kb on - strand, within CCNA_00174 at 190.424 kb on + strand, within CCNA_00174 at 190.432 kb on - strand, within CCNA_00174 at 190.450 kb on + strand, within CCNA_00174 at 190.457 kb on + strand, within CCNA_00174 at 190.505 kb on + strand, within CCNA_00174 at 190.540 kb on + strand, within CCNA_00174 at 190.603 kb on + strand, within CCNA_00174 at 190.624 kb on - strand, within CCNA_00174 at 190.624 kb on - strand, within CCNA_00174 at 190.631 kb on - strand, within CCNA_00174 at 190.661 kb on + strand, within CCNA_00174 at 190.661 kb on + strand, within CCNA_00174 at 190.669 kb on - strand, within CCNA_00174 at 190.678 kb on - strand, within CCNA_00174 at 190.684 kb on - strand, within CCNA_00174 at 190.694 kb on + strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.696 kb on + strand, within CCNA_00174 at 190.704 kb on - strand, within CCNA_00174 at 190.704 kb on - strand, within CCNA_00174 at 190.708 kb on + strand, within CCNA_00174 at 190.709 kb on - strand, within CCNA_00174 at 190.709 kb on - strand, within CCNA_00174 at 190.716 kb on - strand, within CCNA_00174 at 190.716 kb on - strand, within CCNA_00174 at 190.762 kb on + strand, within CCNA_00174 at 190.762 kb on + strand, within CCNA_00174 at 190.762 kb on + strand, within CCNA_00174 at 190.837 kb on - strand, within CCNA_00174 at 190.840 kb on + strand, within CCNA_00174 at 190.848 kb on - strand, within CCNA_00174 at 190.850 kb on + strand, within CCNA_00174 at 190.858 kb on - strand, within CCNA_00174 at 190.858 kb on - strand, within CCNA_00174 at 190.915 kb on - strand at 190.915 kb on - strand at 191.009 kb on + strand at 191.022 kb on - strand at 191.037 kb on - strand at 191.081 kb on + strand, within CCNA_00175 at 191.081 kb on + strand, within CCNA_00175 at 191.089 kb on - strand, within CCNA_00175 at 191.089 kb on - strand, within CCNA_00175 at 191.096 kb on + strand, within CCNA_00175 at 191.097 kb on + strand, within CCNA_00175 at 191.099 kb on - strand, within CCNA_00175 at 191.220 kb on - strand, within CCNA_00175 at 191.229 kb on - strand, within CCNA_00175 at 191.268 kb on + strand, within CCNA_00175 at 191.300 kb on + strand, within CCNA_00175 at 191.300 kb on + strand, within CCNA_00175 at 191.307 kb on - strand, within CCNA_00175 at 191.312 kb on - strand, within CCNA_00175 at 191.312 kb on - strand, within CCNA_00175 at 191.357 kb on - strand, within CCNA_00175 at 191.357 kb on - strand, within CCNA_00175 at 191.357 kb on - strand, within CCNA_00175 at 191.357 kb on - strand, within CCNA_00175 at 191.410 kb on - strand at 191.434 kb on - strand at 191.452 kb on + strand at 191.493 kb on - strand, within CCNA_00176 at 191.493 kb on - strand, within CCNA_00176 at 191.494 kb on + strand, within CCNA_00176 at 191.502 kb on - strand, within CCNA_00176 at 191.513 kb on + strand, within CCNA_00176 at 191.518 kb on + strand, within CCNA_00176 at 191.523 kb on + strand, within CCNA_00176 at 191.523 kb on + strand, within CCNA_00176 at 191.523 kb on + strand, within CCNA_00176 at 191.531 kb on - strand, within CCNA_00176 at 191.531 kb on - strand, within CCNA_00176 at 191.531 kb on - strand, within CCNA_00176 at 191.533 kb on + strand, within CCNA_00176 at 191.539 kb on + strand, within CCNA_00176 at 191.547 kb on - strand, within CCNA_00176 at 191.572 kb on + strand, within CCNA_00176 at 191.584 kb on + strand, within CCNA_00176 at 191.585 kb on + strand, within CCNA_00176 at 191.594 kb on - strand, within CCNA_00176 at 191.594 kb on - strand, within CCNA_00176 at 191.618 kb on + strand, within CCNA_00176 at 191.672 kb on + strand, within CCNA_00176 at 191.677 kb on + strand, within CCNA_00176 at 191.703 kb on - strand, within CCNA_00176 at 191.753 kb on + strand, within CCNA_00176 at 191.761 kb on - strand, within CCNA_00176 at 191.788 kb on + strand, within CCNA_00176 at 191.796 kb on - strand, within CCNA_00176 at 191.807 kb on - strand, within CCNA_00176 at 191.843 kb on - strand, within CCNA_00176 at 191.849 kb on + strand, within CCNA_00176 at 191.857 kb on - strand, within CCNA_00176 at 191.882 kb on - strand, within CCNA_00176 at 191.919 kb on + strand at 191.962 kb on + strand at 192.018 kb on + strand, within CCNA_00177
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Chloride 100 mM remove 188,838 + CCNA_00173 0.33 +1.3 188,838 + CCNA_00173 0.33 +0.6 188,844 + CCNA_00173 0.34 -1.1 188,846 - CCNA_00173 0.34 +0.4 188,846 - CCNA_00173 0.34 +0.6 188,846 - CCNA_00173 0.34 -1.9 188,846 - CCNA_00173 0.34 +0.6 188,846 - CCNA_00173 0.34 -1.8 188,846 - CCNA_00173 0.34 +1.3 188,852 - +1.6 188,925 + CCNA_00173 0.39 -1.2 188,929 - CCNA_00173 0.39 +1.3 188,935 + CCNA_00173 0.40 -1.7 188,971 - CCNA_00173 0.42 -1.9 188,996 + CCNA_00173 0.44 -1.2 189,001 + CCNA_00173 0.44 -1.6 189,009 - CCNA_00173 0.45 -0.5 189,011 + CCNA_00173 0.45 -0.7 189,011 - CCNA_00173 0.45 -2.3 189,016 - CCNA_00173 0.45 -0.4 189,018 + CCNA_00173 0.45 +0.0 189,020 - CCNA_00173 0.46 -0.4 189,025 - CCNA_00173 0.46 +1.3 189,051 + CCNA_00173 0.48 -0.4 189,187 + CCNA_00173 0.57 -0.4 189,199 + CCNA_00173 0.58 -1.9 189,211 + CCNA_00173 0.59 -1.5 189,216 + CCNA_00173 0.59 -1.7 189,219 - CCNA_00173 0.59 -0.2 189,222 + CCNA_00173 0.59 -0.6 189,222 + CCNA_00173 0.59 -0.9 189,238 + CCNA_00173 0.60 -1.0 189,263 + CCNA_00173 0.62 -2.9 189,281 + CCNA_00173 0.63 +1.0 189,325 + CCNA_00173 0.66 +0.8 189,337 + CCNA_00173 0.67 -0.7 189,346 + CCNA_00173 0.68 -0.8 189,403 + CCNA_00173 0.72 -0.8 189,406 - CCNA_00173 0.72 -2.6 189,444 + CCNA_00173 0.74 -1.5 189,444 + CCNA_00173 0.74 -0.3 189,452 - CCNA_00173 0.75 -0.8 189,454 - CCNA_00173 0.75 -0.4 189,483 - CCNA_00173 0.77 -3.0 189,538 + CCNA_00173 0.81 -1.0 189,552 + CCNA_00173 0.82 +1.1 189,560 - CCNA_00173 0.82 -1.6 189,603 + CCNA_00173 0.85 +0.4 189,632 + CCNA_00173 0.87 +2.0 189,637 + CCNA_00173 0.87 -0.3 189,638 - CCNA_00173 0.87 -1.3 189,640 - CCNA_00173 0.88 -2.7 189,655 + CCNA_00173 0.89 +0.8 189,663 - CCNA_00173 0.89 +0.0 189,688 + +0.6 189,718 - -1.8 189,730 - -1.1 189,748 + -2.4 189,751 - -1.0 189,756 - -0.5 189,766 - -3.0 189,777 - -2.8 189,815 + +1.0 189,816 + +0.5 189,816 + +0.7 189,829 + -1.2 189,873 - +0.1 189,873 - -1.4 189,894 - +0.0 189,974 + CCNA_00174 0.14 -1.8 189,994 - CCNA_00174 0.15 -0.7 189,994 - CCNA_00174 0.15 -1.7 190,050 + CCNA_00174 0.20 -0.9 190,052 + CCNA_00174 0.20 -1.6 190,054 + CCNA_00174 0.20 -0.5 190,056 + CCNA_00174 0.20 -0.4 190,066 + CCNA_00174 0.21 +0.3 190,100 - CCNA_00174 0.24 +0.6 190,120 + CCNA_00174 0.26 +0.4 190,121 - CCNA_00174 0.26 -1.4 190,133 + CCNA_00174 0.27 -1.4 190,143 - CCNA_00174 0.28 -1.4 190,143 - CCNA_00174 0.28 -0.2 190,143 - CCNA_00174 0.28 -1.8 190,161 - CCNA_00174 0.29 -2.1 190,198 - CCNA_00174 0.32 -1.1 190,203 - CCNA_00174 0.33 +0.6 190,237 + CCNA_00174 0.35 +0.7 190,280 + CCNA_00174 0.39 -1.8 190,307 + CCNA_00174 0.41 +0.2 190,307 + CCNA_00174 0.41 +0.1 190,307 + CCNA_00174 0.41 -1.9 190,307 + CCNA_00174 0.41 -1.1 190,307 + CCNA_00174 0.41 -2.6 190,312 + CCNA_00174 0.42 -1.9 190,315 - CCNA_00174 0.42 +0.1 190,315 - CCNA_00174 0.42 -2.6 190,315 - CCNA_00174 0.42 -0.9 190,315 - CCNA_00174 0.42 -1.5 190,315 - +2.0 190,315 - CCNA_00174 0.42 +0.4 190,320 - CCNA_00174 0.42 +1.3 190,378 + CCNA_00174 0.47 -1.3 190,385 - CCNA_00174 0.48 +0.1 190,409 + CCNA_00174 0.50 -3.1 190,417 - CCNA_00174 0.50 -2.7 190,424 + CCNA_00174 0.51 -1.7 190,432 - CCNA_00174 0.51 +1.0 190,450 + CCNA_00174 0.53 -0.2 190,457 + CCNA_00174 0.53 +0.1 190,505 + CCNA_00174 0.57 +0.8 190,540 + CCNA_00174 0.60 -1.0 190,603 + CCNA_00174 0.66 -1.1 190,624 - CCNA_00174 0.67 -1.0 190,624 - CCNA_00174 0.67 +0.6 190,631 - CCNA_00174 0.68 -1.4 190,661 + CCNA_00174 0.70 -2.2 190,661 + CCNA_00174 0.70 -0.6 190,669 - CCNA_00174 0.71 +0.1 190,678 - CCNA_00174 0.72 -1.9 190,684 - CCNA_00174 0.72 -0.6 190,694 + CCNA_00174 0.73 -1.9 190,696 + CCNA_00174 0.73 +0.6 190,696 + CCNA_00174 0.73 -0.7 190,696 + CCNA_00174 0.73 -0.6 190,696 + CCNA_00174 0.73 +0.0 190,696 + CCNA_00174 0.73 -0.9 190,696 + CCNA_00174 0.73 -1.2 190,704 - CCNA_00174 0.74 -1.7 190,704 - CCNA_00174 0.74 -1.5 190,708 + CCNA_00174 0.74 -1.2 190,709 - CCNA_00174 0.74 -2.1 190,709 - CCNA_00174 0.74 -0.0 190,716 - CCNA_00174 0.75 -1.4 190,716 - CCNA_00174 0.75 -1.1 190,762 + CCNA_00174 0.79 -2.2 190,762 + CCNA_00174 0.79 +0.0 190,762 + CCNA_00174 0.79 -1.4 190,837 - CCNA_00174 0.85 -2.2 190,840 + CCNA_00174 0.85 -2.3 190,848 - CCNA_00174 0.86 -1.2 190,850 + CCNA_00174 0.86 +0.3 190,858 - CCNA_00174 0.87 -1.6 190,858 - CCNA_00174 0.87 -1.2 190,915 - -1.6 190,915 - -1.4 191,009 + -2.7 191,022 - +1.2 191,037 - +0.2 191,081 + CCNA_00175 0.11 +1.0 191,081 + CCNA_00175 0.11 -0.2 191,089 - CCNA_00175 0.13 +0.0 191,089 - CCNA_00175 0.13 +0.0 191,096 + CCNA_00175 0.14 -0.6 191,097 + CCNA_00175 0.14 -0.8 191,099 - CCNA_00175 0.15 -0.9 191,220 - CCNA_00175 0.42 -0.0 191,229 - CCNA_00175 0.43 -0.2 191,268 + CCNA_00175 0.52 -1.0 191,300 + CCNA_00175 0.59 +0.0 191,300 + CCNA_00175 0.59 -1.0 191,307 - CCNA_00175 0.61 -1.3 191,312 - CCNA_00175 0.62 +0.8 191,312 - CCNA_00175 0.62 -0.1 191,357 - CCNA_00175 0.72 +0.5 191,357 - CCNA_00175 0.72 +0.3 191,357 - CCNA_00175 0.72 -0.1 191,357 - CCNA_00175 0.72 +0.0 191,410 - -1.4 191,434 - -0.2 191,452 + -0.4 191,493 - CCNA_00176 0.17 -0.8 191,493 - CCNA_00176 0.17 -0.8 191,494 + CCNA_00176 0.17 -1.1 191,502 - CCNA_00176 0.18 -2.3 191,513 + CCNA_00176 0.20 -3.5 191,518 + CCNA_00176 0.21 -0.6 191,523 + CCNA_00176 0.22 -0.4 191,523 + CCNA_00176 0.22 +0.8 191,523 + CCNA_00176 0.22 +0.0 191,531 - CCNA_00176 0.24 -0.1 191,531 - CCNA_00176 0.24 -0.6 191,531 - CCNA_00176 0.24 -1.7 191,533 + CCNA_00176 0.24 -2.5 191,539 + CCNA_00176 0.25 -2.1 191,547 - CCNA_00176 0.27 -1.9 191,572 + CCNA_00176 0.31 -1.4 191,584 + CCNA_00176 0.33 +0.3 191,585 + CCNA_00176 0.33 -0.7 191,594 - CCNA_00176 0.35 -0.2 191,594 - CCNA_00176 0.35 -0.9 191,618 + CCNA_00176 0.39 -2.3 191,672 + CCNA_00176 0.49 -1.7 191,677 + CCNA_00176 0.50 -3.4 191,703 - CCNA_00176 0.54 +1.2 191,753 + CCNA_00176 0.63 -1.9 191,761 - CCNA_00176 0.65 -1.4 191,788 + CCNA_00176 0.70 -0.2 191,796 - CCNA_00176 0.71 -0.5 191,807 - CCNA_00176 0.73 -1.5 191,843 - CCNA_00176 0.80 -1.0 191,849 + CCNA_00176 0.81 -2.3 191,857 - CCNA_00176 0.82 +0.0 191,882 - CCNA_00176 0.87 -0.2 191,919 + -0.2 191,962 + -1.1 192,018 + CCNA_00177 0.24 +0.0
Or see this region's nucleotide sequence