Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_877 and Pf1N1B4_878 overlap by 49 nucleotides Pf1N1B4_878 and Pf1N1B4_879 are separated by 112 nucleotides Pf1N1B4_879 and Pf1N1B4_880 are separated by 94 nucleotides Pf1N1B4_880 and Pf1N1B4_881 are separated by 211 nucleotides Pf1N1B4_881 and Pf1N1B4_882 overlap by 4 nucleotides
Pf1N1B4_877 - Permeases of the major facilitator superfamily, at 1,003,052 to 1,003,864
_877
Pf1N1B4_878 - Permeases of the major facilitator superfamily, at 1,003,816 to 1,004,367
_878
Pf1N1B4_879 - Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-), at 1,004,480 to 1,005,661
_879
Pf1N1B4_880 - Putative preQ0 transporter, at 1,005,756 to 1,006,229
_880
Pf1N1B4_881 - FIG00954164: hypothetical protein, at 1,006,441 to 1,006,656
_881
Pf1N1B4_882 - carbonic anhydrase, family 3, at 1,006,653 to 1,007,177
_882
Position (kb)
1004
1005
1006 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.688 kb on + strand at 1003.688 kb on + strand at 1003.688 kb on + strand, within Pf1N1B4_877 at 1003.689 kb on - strand, within Pf1N1B4_877 at 1003.689 kb on - strand, within Pf1N1B4_877 at 1003.689 kb on - strand, within Pf1N1B4_877 at 1003.689 kb on - strand, within Pf1N1B4_877 at 1003.689 kb on - strand, within Pf1N1B4_877 at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.840 kb on + strand at 1003.841 kb on - strand at 1003.841 kb on - strand at 1003.841 kb on - strand at 1003.841 kb on - strand at 1003.841 kb on - strand at 1003.841 kb on - strand at 1003.841 kb on - strand at 1003.841 kb on - strand at 1003.841 kb on - strand at 1003.842 kb on + strand at 1003.842 kb on + strand at 1003.843 kb on - strand at 1003.843 kb on - strand at 1003.867 kb on + strand at 1003.867 kb on + strand at 1003.867 kb on + strand at 1003.867 kb on + strand at 1003.867 kb on + strand at 1003.867 kb on + strand at 1003.867 kb on + strand at 1003.867 kb on + strand at 1003.867 kb on + strand at 1003.868 kb on - strand at 1003.868 kb on - strand at 1003.868 kb on - strand at 1003.868 kb on - strand at 1003.868 kb on - strand at 1003.868 kb on - strand at 1004.191 kb on + strand, within Pf1N1B4_878 at 1004.191 kb on + strand, within Pf1N1B4_878 at 1004.270 kb on - strand, within Pf1N1B4_878 at 1004.343 kb on + strand at 1004.414 kb on - strand at 1004.414 kb on - strand at 1004.489 kb on + strand at 1004.489 kb on + strand at 1004.489 kb on + strand at 1004.490 kb on - strand at 1004.490 kb on - strand at 1004.490 kb on - strand at 1004.974 kb on - strand, within Pf1N1B4_879 at 1005.124 kb on - strand at 1005.240 kb on + strand, within Pf1N1B4_879 at 1005.240 kb on + strand, within Pf1N1B4_879 at 1005.241 kb on - strand, within Pf1N1B4_879 at 1005.241 kb on - strand, within Pf1N1B4_879 at 1005.264 kb on + strand at 1005.264 kb on + strand, within Pf1N1B4_879 at 1005.264 kb on + strand at 1005.264 kb on + strand, within Pf1N1B4_879 at 1005.265 kb on - strand, within Pf1N1B4_879 at 1005.265 kb on - strand, within Pf1N1B4_879 at 1005.265 kb on - strand, within Pf1N1B4_879 at 1005.265 kb on - strand, within Pf1N1B4_879 at 1005.267 kb on + strand, within Pf1N1B4_879 at 1005.617 kb on + strand at 1005.617 kb on + strand at 1005.618 kb on - strand at 1005.740 kb on - strand at 1005.740 kb on - strand at 1005.782 kb on + strand at 1005.866 kb on + strand, within Pf1N1B4_880 at 1005.866 kb on + strand, within Pf1N1B4_880 at 1005.928 kb on - strand, within Pf1N1B4_880 at 1006.045 kb on + strand, within Pf1N1B4_880 at 1006.045 kb on + strand, within Pf1N1B4_880 at 1006.045 kb on + strand, within Pf1N1B4_880 at 1006.045 kb on + strand, within Pf1N1B4_880 at 1006.045 kb on + strand, within Pf1N1B4_880 at 1006.045 kb on + strand, within Pf1N1B4_880 at 1006.045 kb on + strand, within Pf1N1B4_880 at 1006.045 kb on + strand, within Pf1N1B4_880 at 1006.045 kb on + strand, within Pf1N1B4_880 at 1006.046 kb on - strand, within Pf1N1B4_880 at 1006.046 kb on - strand, within Pf1N1B4_880 at 1006.046 kb on - strand, within Pf1N1B4_880 at 1006.046 kb on - strand, within Pf1N1B4_880 at 1006.046 kb on - strand, within Pf1N1B4_880 at 1006.046 kb on - strand, within Pf1N1B4_880 at 1006.046 kb on - strand, within Pf1N1B4_880 at 1006.210 kb on + strand at 1006.241 kb on + strand at 1006.241 kb on + strand at 1006.241 kb on + strand at 1006.241 kb on + strand at 1006.242 kb on - strand at 1006.242 kb on - strand at 1006.242 kb on - strand at 1006.266 kb on + strand at 1006.267 kb on - strand at 1006.284 kb on + strand at 1006.284 kb on + strand at 1006.284 kb on + strand at 1006.285 kb on - strand at 1006.285 kb on - strand at 1006.285 kb on - strand at 1006.285 kb on - strand at 1006.288 kb on + strand at 1006.288 kb on + strand at 1006.363 kb on + strand at 1006.433 kb on + strand at 1006.434 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 1,003,688 + Pf1N1B4_877 0.78 -2.6 1,003,688 + Pf1N1B4_877 0.78 -0.5 1,003,688 + Pf1N1B4_877 0.78 -2.1 1,003,688 + Pf1N1B4_877 0.78 +1.5 1,003,688 + Pf1N1B4_877 0.78 +0.4 1,003,688 + Pf1N1B4_877 0.78 +0.4 1,003,688 + Pf1N1B4_877 0.78 -0.6 1,003,688 + +3.4 1,003,688 + +0.1 1,003,688 + Pf1N1B4_877 0.78 -0.2 1,003,689 - Pf1N1B4_877 0.78 -0.7 1,003,689 - Pf1N1B4_877 0.78 -0.5 1,003,689 - Pf1N1B4_877 0.78 -0.5 1,003,689 - Pf1N1B4_877 0.78 -0.9 1,003,689 - Pf1N1B4_877 0.78 +0.5 1,003,840 + -1.2 1,003,840 + -0.9 1,003,840 + +0.4 1,003,840 + +0.1 1,003,840 + +0.2 1,003,840 + +0.3 1,003,840 + +0.1 1,003,840 + -0.2 1,003,840 + -2.2 1,003,840 + -0.4 1,003,840 + -0.1 1,003,840 + -0.6 1,003,840 + -1.9 1,003,840 + +0.5 1,003,840 + -1.7 1,003,841 - +0.7 1,003,841 - +0.4 1,003,841 - -0.3 1,003,841 - -0.9 1,003,841 - -0.5 1,003,841 - +0.1 1,003,841 - -1.1 1,003,841 - -1.1 1,003,841 - -0.9 1,003,842 + -0.1 1,003,842 + +0.1 1,003,843 - -0.5 1,003,843 - -0.1 1,003,867 + +0.1 1,003,867 + -1.1 1,003,867 + +0.9 1,003,867 + +0.1 1,003,867 + +0.6 1,003,867 + -2.3 1,003,867 + -0.0 1,003,867 + +1.5 1,003,867 + -1.0 1,003,868 - +1.5 1,003,868 - -0.5 1,003,868 - +0.7 1,003,868 - +0.3 1,003,868 - +0.5 1,003,868 - -0.9 1,004,191 + Pf1N1B4_878 0.68 -1.2 1,004,191 + Pf1N1B4_878 0.68 -0.9 1,004,270 - Pf1N1B4_878 0.82 +1.1 1,004,343 + +0.1 1,004,414 - +0.1 1,004,414 - +0.8 1,004,489 + +0.5 1,004,489 + -0.5 1,004,489 + -0.1 1,004,490 - -0.5 1,004,490 - -0.3 1,004,490 - +1.4 1,004,974 - Pf1N1B4_879 0.42 -0.7 1,005,124 - -0.2 1,005,240 + Pf1N1B4_879 0.64 +0.2 1,005,240 + Pf1N1B4_879 0.64 -1.5 1,005,241 - Pf1N1B4_879 0.64 +1.3 1,005,241 - Pf1N1B4_879 0.64 -1.7 1,005,264 + -0.5 1,005,264 + Pf1N1B4_879 0.66 -3.3 1,005,264 + +1.1 1,005,264 + Pf1N1B4_879 0.66 -2.3 1,005,265 - Pf1N1B4_879 0.66 -1.2 1,005,265 - Pf1N1B4_879 0.66 +0.3 1,005,265 - Pf1N1B4_879 0.66 -0.9 1,005,265 - Pf1N1B4_879 0.66 -0.5 1,005,267 + Pf1N1B4_879 0.67 -1.2 1,005,617 + +1.1 1,005,617 + -0.5 1,005,618 - -1.5 1,005,740 - +1.3 1,005,740 - -0.2 1,005,782 + +1.1 1,005,866 + Pf1N1B4_880 0.23 +0.1 1,005,866 + Pf1N1B4_880 0.23 +0.3 1,005,928 - Pf1N1B4_880 0.36 +0.1 1,006,045 + Pf1N1B4_880 0.61 -1.7 1,006,045 + Pf1N1B4_880 0.61 -1.9 1,006,045 + Pf1N1B4_880 0.61 +0.5 1,006,045 + Pf1N1B4_880 0.61 -0.5 1,006,045 + Pf1N1B4_880 0.61 -0.2 1,006,045 + Pf1N1B4_880 0.61 -0.3 1,006,045 + Pf1N1B4_880 0.61 -0.5 1,006,045 + Pf1N1B4_880 0.61 -0.1 1,006,045 + Pf1N1B4_880 0.61 -0.3 1,006,046 - Pf1N1B4_880 0.61 -0.0 1,006,046 - Pf1N1B4_880 0.61 +0.1 1,006,046 - Pf1N1B4_880 0.61 -1.7 1,006,046 - Pf1N1B4_880 0.61 -0.7 1,006,046 - Pf1N1B4_880 0.61 -0.5 1,006,046 - Pf1N1B4_880 0.61 -0.5 1,006,046 - Pf1N1B4_880 0.61 +1.1 1,006,210 + +0.2 1,006,241 + +0.1 1,006,241 + +0.4 1,006,241 + +0.8 1,006,241 + -0.2 1,006,242 - +1.0 1,006,242 - +1.5 1,006,242 - -0.6 1,006,266 + +0.5 1,006,267 - -2.3 1,006,284 + -0.9 1,006,284 + +0.9 1,006,284 + -0.1 1,006,285 - -1.5 1,006,285 - -0.4 1,006,285 - +0.5 1,006,285 - -2.2 1,006,288 + -1.3 1,006,288 + -0.0 1,006,363 + -1.2 1,006,433 + +1.1 1,006,434 - -0.9
Or see this region's nucleotide sequence