Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_744 and Pf1N1B4_745 overlap by 4 nucleotides Pf1N1B4_745 and Pf1N1B4_746 are separated by 116 nucleotides
Pf1N1B4_744 - Dihydroorotase (EC 3.5.2.3), at 846,721 to 847,767
_744
Pf1N1B4_745 - Ribonuclease T (EC 3.1.13.-), at 847,764 to 848,441
_745
Pf1N1B4_746 - Alkyl hydroperoxide reductase subunit C-like protein, at 848,558 to 849,160
_746
Position (kb)
847
848
849 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 848.430 kb on + strand at 848.430 kb on + strand at 848.430 kb on + strand at 848.430 kb on + strand at 848.430 kb on + strand at 848.431 kb on - strand at 848.431 kb on - strand at 848.450 kb on + strand at 848.560 kb on - strand at 848.560 kb on - strand at 848.586 kb on + strand at 848.714 kb on - strand, within Pf1N1B4_746 at 848.817 kb on + strand, within Pf1N1B4_746 at 848.817 kb on + strand, within Pf1N1B4_746 at 848.818 kb on - strand, within Pf1N1B4_746 at 849.052 kb on - strand, within Pf1N1B4_746 at 849.177 kb on + strand at 849.177 kb on + strand at 849.178 kb on - strand at 849.178 kb on - strand at 849.178 kb on - strand at 849.178 kb on - strand at 849.178 kb on - strand at 849.178 kb on - strand at 849.181 kb on - strand at 849.181 kb on - strand at 849.181 kb on - strand at 849.269 kb on + strand at 849.269 kb on + strand at 849.269 kb on + strand at 849.270 kb on - strand at 849.270 kb on - strand at 849.270 kb on - strand at 849.270 kb on - strand at 849.270 kb on - strand at 849.270 kb on - strand at 849.270 kb on - strand at 849.270 kb on - strand at 849.270 kb on - strand at 849.270 kb on - strand at 849.270 kb on - strand at 849.324 kb on - strand at 849.338 kb on + strand at 849.338 kb on + strand at 849.339 kb on - strand at 849.339 kb on - strand at 849.340 kb on + strand at 849.340 kb on + strand at 849.340 kb on + strand at 849.341 kb on - strand at 849.391 kb on + strand at 849.391 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 848,430 + +1.4 848,430 + +0.6 848,430 + -0.0 848,430 + -0.4 848,430 + +0.7 848,431 - +0.1 848,431 - -1.8 848,450 + +1.1 848,560 - -1.5 848,560 - -0.5 848,586 + +0.4 848,714 - Pf1N1B4_746 0.26 +1.1 848,817 + Pf1N1B4_746 0.43 +2.1 848,817 + Pf1N1B4_746 0.43 +1.1 848,818 - Pf1N1B4_746 0.43 +0.1 849,052 - Pf1N1B4_746 0.82 +0.1 849,177 + +1.4 849,177 + +0.1 849,178 - -2.7 849,178 - +0.5 849,178 - -0.1 849,178 - +0.5 849,178 - +0.1 849,178 - +0.3 849,181 - -0.9 849,181 - -1.9 849,181 - -0.3 849,269 + -0.1 849,269 + +1.6 849,269 + -1.3 849,270 - +1.1 849,270 - -1.5 849,270 - -1.3 849,270 - +0.7 849,270 - -2.3 849,270 - -0.9 849,270 - +3.4 849,270 - -1.8 849,270 - -0.6 849,270 - -2.1 849,270 - +0.1 849,324 - +0.6 849,338 + +0.5 849,338 + +2.1 849,339 - -1.1 849,339 - +1.1 849,340 + +0.5 849,340 + -1.3 849,340 + -1.3 849,341 - -1.4 849,391 + -0.3 849,391 + -0.5
Or see this region's nucleotide sequence