Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_679 and Pf1N1B4_680 are separated by 134 nucleotides Pf1N1B4_680 and Pf1N1B4_681 overlap by 8 nucleotides
Pf1N1B4_679 - Enoyl-CoA hydratase (EC 4.2.1.17), at 776,267 to 777,016
_679
Pf1N1B4_680 - 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases, at 777,151 to 778,086
_680
Pf1N1B4_681 - FOG: GGDEF domain, at 778,079 to 779,185
_681
Position (kb) 777
778
779 Strain fitness (log2 ratio) -2
-1
0
1 at 776.281 kb on + strand at 776.282 kb on - strand at 776.282 kb on - strand at 776.665 kb on - strand, within Pf1N1B4_679 at 776.773 kb on - strand, within Pf1N1B4_679 at 776.922 kb on + strand, within Pf1N1B4_679 at 776.922 kb on + strand, within Pf1N1B4_679 at 776.922 kb on + strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 776.923 kb on - strand, within Pf1N1B4_679 at 777.163 kb on + strand at 777.209 kb on + strand at 777.210 kb on - strand at 777.210 kb on - strand at 777.383 kb on + strand, within Pf1N1B4_680 at 777.383 kb on + strand, within Pf1N1B4_680 at 777.384 kb on - strand, within Pf1N1B4_680 at 777.534 kb on - strand, within Pf1N1B4_680 at 777.680 kb on + strand, within Pf1N1B4_680 at 777.761 kb on - strand, within Pf1N1B4_680 at 777.990 kb on + strand at 777.991 kb on - strand, within Pf1N1B4_680 at 777.991 kb on - strand, within Pf1N1B4_680 at 777.991 kb on - strand, within Pf1N1B4_680 at 777.991 kb on - strand, within Pf1N1B4_680 at 777.993 kb on - strand at 778.266 kb on - strand, within Pf1N1B4_681 at 778.651 kb on + strand, within Pf1N1B4_681 at 778.712 kb on + strand, within Pf1N1B4_681 at 778.712 kb on + strand at 778.713 kb on - strand, within Pf1N1B4_681 at 778.713 kb on - strand, within Pf1N1B4_681 at 778.873 kb on - strand, within Pf1N1B4_681 at 778.873 kb on - strand, within Pf1N1B4_681 at 778.947 kb on - strand, within Pf1N1B4_681 at 778.947 kb on - strand, within Pf1N1B4_681 at 779.076 kb on + strand at 779.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 776,281 + +1.0 776,282 - -0.3 776,282 - -0.9 776,665 - Pf1N1B4_679 0.53 +0.2 776,773 - Pf1N1B4_679 0.67 +0.2 776,922 + Pf1N1B4_679 0.87 -0.7 776,922 + Pf1N1B4_679 0.87 -1.3 776,922 + Pf1N1B4_679 0.87 -0.1 776,923 - Pf1N1B4_679 0.87 -0.5 776,923 - Pf1N1B4_679 0.87 +0.0 776,923 - Pf1N1B4_679 0.87 +1.0 776,923 - Pf1N1B4_679 0.87 +0.4 777,163 + +0.7 777,209 + +0.4 777,210 - +0.8 777,210 - -0.6 777,383 + Pf1N1B4_680 0.25 -0.9 777,383 + Pf1N1B4_680 0.25 -0.5 777,384 - Pf1N1B4_680 0.25 -0.6 777,534 - Pf1N1B4_680 0.41 +1.0 777,680 + Pf1N1B4_680 0.57 -0.3 777,761 - Pf1N1B4_680 0.65 -2.0 777,990 + +1.0 777,991 - Pf1N1B4_680 0.90 -1.4 777,991 - Pf1N1B4_680 0.90 -0.7 777,991 - Pf1N1B4_680 0.90 -2.3 777,991 - Pf1N1B4_680 0.90 -0.3 777,993 - +0.0 778,266 - Pf1N1B4_681 0.17 -2.3 778,651 + Pf1N1B4_681 0.52 +0.6 778,712 + Pf1N1B4_681 0.57 +0.7 778,712 + +1.3 778,713 - Pf1N1B4_681 0.57 -1.2 778,713 - Pf1N1B4_681 0.57 +0.0 778,873 - Pf1N1B4_681 0.72 +0.0 778,873 - Pf1N1B4_681 0.72 +0.6 778,947 - Pf1N1B4_681 0.78 +0.4 778,947 - Pf1N1B4_681 0.78 +0.2 779,076 + -1.4 779,077 - -0.4
Or see this region's nucleotide sequence