Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_644 and Pf1N1B4_645 are separated by 76 nucleotides Pf1N1B4_645 and Pf1N1B4_646 are separated by 3 nucleotides Pf1N1B4_646 and Pf1N1B4_647 are separated by 689 nucleotides Pf1N1B4_647 and Pf1N1B4_648 are separated by 6 nucleotides
Pf1N1B4_644 - Cold shock protein CspC, at 739,324 to 739,926
_644
Pf1N1B4_645 - Protein SlyX, at 740,003 to 740,209
_645
Pf1N1B4_646 - Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases, at 740,213 to 740,638
_646
Pf1N1B4_647 - hypothetical protein, at 741,328 to 741,531
_647
Pf1N1B4_648 - Outer membrane porin, OprD family, at 741,538 to 742,866
_648
Position (kb) 740
741 Strain fitness (log2 ratio) -1
0
1
2
3 at 739.223 kb on - strand at 739.347 kb on - strand at 739.409 kb on - strand, within Pf1N1B4_644 at 739.699 kb on + strand, within Pf1N1B4_644 at 739.700 kb on - strand, within Pf1N1B4_644 at 739.764 kb on + strand, within Pf1N1B4_644 at 739.765 kb on - strand, within Pf1N1B4_644 at 739.937 kb on - strand at 739.978 kb on - strand at 740.007 kb on + strand at 740.007 kb on + strand at 740.007 kb on + strand at 740.007 kb on + strand at 740.008 kb on - strand at 740.008 kb on - strand at 740.008 kb on - strand at 740.008 kb on - strand at 740.293 kb on - strand, within Pf1N1B4_646 at 740.293 kb on - strand, within Pf1N1B4_646 at 740.293 kb on - strand, within Pf1N1B4_646 at 740.293 kb on - strand, within Pf1N1B4_646 at 740.293 kb on - strand, within Pf1N1B4_646 at 740.293 kb on - strand, within Pf1N1B4_646 at 740.390 kb on - strand, within Pf1N1B4_646 at 740.484 kb on - strand, within Pf1N1B4_646 at 740.484 kb on - strand, within Pf1N1B4_646 at 740.484 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.493 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.502 kb on - strand, within Pf1N1B4_646 at 740.548 kb on - strand, within Pf1N1B4_646 at 740.548 kb on - strand, within Pf1N1B4_646 at 740.548 kb on - strand, within Pf1N1B4_646 at 740.548 kb on - strand, within Pf1N1B4_646 at 740.628 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.723 kb on - strand at 740.806 kb on - strand at 740.979 kb on - strand at 740.979 kb on - strand at 740.979 kb on - strand at 740.979 kb on - strand at 740.979 kb on - strand at 740.979 kb on - strand at 741.040 kb on - strand at 741.040 kb on - strand at 741.040 kb on - strand at 741.040 kb on - strand at 741.040 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.074 kb on - strand at 741.100 kb on - strand at 741.111 kb on - strand at 741.194 kb on - strand at 741.194 kb on - strand at 741.194 kb on - strand at 741.196 kb on - strand at 741.223 kb on - strand at 741.232 kb on - strand at 741.367 kb on - strand, within Pf1N1B4_647 at 741.409 kb on - strand, within Pf1N1B4_647 at 741.409 kb on - strand, within Pf1N1B4_647 at 741.409 kb on - strand, within Pf1N1B4_647 at 741.409 kb on - strand, within Pf1N1B4_647 at 741.421 kb on - strand, within Pf1N1B4_647 at 741.444 kb on - strand, within Pf1N1B4_647 at 741.444 kb on - strand, within Pf1N1B4_647 at 741.444 kb on - strand, within Pf1N1B4_647 at 741.444 kb on - strand, within Pf1N1B4_647 at 741.444 kb on - strand, within Pf1N1B4_647 at 741.445 kb on + strand, within Pf1N1B4_647 at 741.445 kb on + strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.446 kb on - strand, within Pf1N1B4_647 at 741.486 kb on + strand, within Pf1N1B4_647 at 741.487 kb on - strand, within Pf1N1B4_647 at 741.487 kb on - strand, within Pf1N1B4_647 at 741.487 kb on - strand, within Pf1N1B4_647 at 741.487 kb on - strand, within Pf1N1B4_647 at 741.487 kb on - strand, within Pf1N1B4_647 at 741.515 kb on - strand at 741.591 kb on + strand at 741.591 kb on + strand at 741.620 kb on + strand at 741.620 kb on + strand at 741.620 kb on + strand at 741.620 kb on + strand at 741.620 kb on + strand at 741.620 kb on + strand at 741.621 kb on - strand at 741.621 kb on - strand at 741.621 kb on - strand at 741.621 kb on - strand at 741.621 kb on - strand at 741.621 kb on - strand at 741.621 kb on - strand at 741.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 739,223 - +0.5 739,347 - -1.0 739,409 - Pf1N1B4_644 0.14 +0.4 739,699 + Pf1N1B4_644 0.62 -0.8 739,700 - Pf1N1B4_644 0.62 -1.0 739,764 + Pf1N1B4_644 0.73 -1.1 739,765 - Pf1N1B4_644 0.73 -0.8 739,937 - -0.3 739,978 - +2.0 740,007 + -0.2 740,007 + +2.2 740,007 + +2.2 740,007 + +0.4 740,008 - +2.2 740,008 - +0.7 740,008 - +1.7 740,008 - +0.4 740,293 - Pf1N1B4_646 0.19 +1.6 740,293 - Pf1N1B4_646 0.19 +0.9 740,293 - Pf1N1B4_646 0.19 +1.8 740,293 - Pf1N1B4_646 0.19 +1.6 740,293 - Pf1N1B4_646 0.19 +0.5 740,293 - Pf1N1B4_646 0.19 +1.1 740,390 - Pf1N1B4_646 0.42 -1.3 740,484 - Pf1N1B4_646 0.64 +0.6 740,484 - Pf1N1B4_646 0.64 +0.7 740,484 - Pf1N1B4_646 0.64 +1.0 740,493 - Pf1N1B4_646 0.66 +2.2 740,493 - Pf1N1B4_646 0.66 -1.0 740,493 - Pf1N1B4_646 0.66 +0.3 740,493 - Pf1N1B4_646 0.66 +0.7 740,493 - Pf1N1B4_646 0.66 +1.7 740,493 - Pf1N1B4_646 0.66 +1.4 740,493 - Pf1N1B4_646 0.66 +1.4 740,493 - Pf1N1B4_646 0.66 +1.4 740,493 - Pf1N1B4_646 0.66 +2.8 740,493 - Pf1N1B4_646 0.66 +1.5 740,493 - Pf1N1B4_646 0.66 +0.6 740,493 - Pf1N1B4_646 0.66 +1.6 740,493 - Pf1N1B4_646 0.66 +1.7 740,493 - Pf1N1B4_646 0.66 +2.3 740,493 - Pf1N1B4_646 0.66 +0.4 740,493 - Pf1N1B4_646 0.66 +0.6 740,502 - Pf1N1B4_646 0.68 +0.8 740,502 - Pf1N1B4_646 0.68 +2.2 740,502 - Pf1N1B4_646 0.68 +1.6 740,502 - Pf1N1B4_646 0.68 +1.0 740,502 - Pf1N1B4_646 0.68 +1.0 740,502 - Pf1N1B4_646 0.68 +0.6 740,502 - Pf1N1B4_646 0.68 +1.0 740,502 - Pf1N1B4_646 0.68 -0.5 740,502 - Pf1N1B4_646 0.68 +0.6 740,502 - Pf1N1B4_646 0.68 +0.8 740,502 - Pf1N1B4_646 0.68 +1.0 740,548 - Pf1N1B4_646 0.79 +1.3 740,548 - Pf1N1B4_646 0.79 +0.8 740,548 - Pf1N1B4_646 0.79 +0.8 740,548 - Pf1N1B4_646 0.79 +1.0 740,628 - +1.3 740,723 - +1.3 740,723 - +1.0 740,723 - -0.0 740,723 - +0.4 740,723 - +2.0 740,723 - +0.2 740,723 - +1.1 740,723 - +0.1 740,723 - +0.8 740,723 - +1.1 740,723 - +0.5 740,723 - +1.2 740,723 - +2.0 740,723 - +0.3 740,723 - +1.0 740,723 - +1.3 740,723 - -0.6 740,723 - +1.3 740,723 - +1.5 740,723 - +1.2 740,806 - +1.6 740,979 - +1.8 740,979 - +1.1 740,979 - +2.8 740,979 - +1.2 740,979 - +1.5 740,979 - +0.8 741,040 - +1.6 741,040 - +1.3 741,040 - +0.2 741,040 - +2.1 741,040 - +1.6 741,074 - +2.4 741,074 - +2.6 741,074 - +1.4 741,074 - +0.7 741,074 - +0.6 741,074 - +1.7 741,074 - +1.1 741,074 - -0.2 741,100 - +1.9 741,111 - +1.0 741,194 - +1.6 741,194 - +0.8 741,194 - +1.2 741,196 - +0.9 741,223 - +1.7 741,232 - +1.8 741,367 - Pf1N1B4_647 0.19 +2.6 741,409 - Pf1N1B4_647 0.40 +1.7 741,409 - Pf1N1B4_647 0.40 +1.5 741,409 - Pf1N1B4_647 0.40 +1.7 741,409 - Pf1N1B4_647 0.40 +1.2 741,421 - Pf1N1B4_647 0.46 +0.4 741,444 - Pf1N1B4_647 0.57 -0.4 741,444 - Pf1N1B4_647 0.57 -0.3 741,444 - Pf1N1B4_647 0.57 +1.2 741,444 - Pf1N1B4_647 0.57 +1.0 741,444 - Pf1N1B4_647 0.57 +2.5 741,445 + Pf1N1B4_647 0.57 +1.7 741,445 + Pf1N1B4_647 0.57 +1.0 741,446 - Pf1N1B4_647 0.58 +2.5 741,446 - Pf1N1B4_647 0.58 +1.9 741,446 - Pf1N1B4_647 0.58 +1.6 741,446 - Pf1N1B4_647 0.58 +2.6 741,446 - Pf1N1B4_647 0.58 +1.0 741,446 - Pf1N1B4_647 0.58 +1.0 741,446 - Pf1N1B4_647 0.58 +1.7 741,446 - Pf1N1B4_647 0.58 +2.4 741,446 - +2.3 741,446 - Pf1N1B4_647 0.58 +0.8 741,446 - Pf1N1B4_647 0.58 +2.7 741,446 - Pf1N1B4_647 0.58 -0.6 741,446 - Pf1N1B4_647 0.58 +1.9 741,446 - Pf1N1B4_647 0.58 +1.5 741,446 - Pf1N1B4_647 0.58 +1.9 741,446 - Pf1N1B4_647 0.58 +2.2 741,486 + Pf1N1B4_647 0.77 +0.2 741,487 - Pf1N1B4_647 0.78 +1.7 741,487 - Pf1N1B4_647 0.78 +2.0 741,487 - Pf1N1B4_647 0.78 +1.0 741,487 - Pf1N1B4_647 0.78 -0.3 741,487 - Pf1N1B4_647 0.78 +1.4 741,515 - +2.4 741,591 + +1.7 741,591 + +0.5 741,620 + +1.6 741,620 + +1.9 741,620 + +1.8 741,620 + +2.6 741,620 + +0.8 741,620 + +1.6 741,621 - +1.5 741,621 - +1.8 741,621 - +1.0 741,621 - +0.4 741,621 - +1.7 741,621 - +1.8 741,621 - +2.2 741,621 - +1.5
Or see this region's nucleotide sequence