Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5948 and Pf1N1B4_5949 are separated by 76 nucleotides Pf1N1B4_5949 and Pf1N1B4_5950 overlap by 4 nucleotides Pf1N1B4_5950 and Pf1N1B4_5951 are separated by 157 nucleotides
Pf1N1B4_5948 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 397,793 to 398,626
_5948
Pf1N1B4_5949 - Phosphomannomutase (EC 5.4.2.8), at 398,703 to 399,134
_5949
Pf1N1B4_5950 - Transcriptional activator RfaH, at 399,131 to 399,637
_5950
Pf1N1B4_5951 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 399,795 to 400,631
_5951
Position (kb) 398
399
400 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 397.774 kb on + strand at 397.888 kb on + strand, within Pf1N1B4_5948 at 397.888 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.943 kb on - strand, within Pf1N1B4_5948 at 397.943 kb on - strand, within Pf1N1B4_5948 at 397.943 kb on - strand, within Pf1N1B4_5948 at 397.943 kb on - strand, within Pf1N1B4_5948 at 398.101 kb on - strand, within Pf1N1B4_5948 at 398.214 kb on + strand, within Pf1N1B4_5948 at 398.238 kb on - strand, within Pf1N1B4_5948 at 398.528 kb on + strand, within Pf1N1B4_5948 at 398.528 kb on + strand at 398.528 kb on + strand, within Pf1N1B4_5948 at 398.529 kb on - strand, within Pf1N1B4_5948 at 398.573 kb on + strand at 398.573 kb on + strand at 398.574 kb on - strand at 398.574 kb on - strand at 398.574 kb on - strand at 398.679 kb on + strand at 398.679 kb on + strand at 398.682 kb on + strand at 398.702 kb on + strand at 398.762 kb on + strand, within Pf1N1B4_5949 at 398.763 kb on - strand, within Pf1N1B4_5949 at 398.778 kb on + strand, within Pf1N1B4_5949 at 399.635 kb on + strand at 399.701 kb on - strand at 399.796 kb on + strand at 399.896 kb on - strand, within Pf1N1B4_5951 at 399.899 kb on + strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 400.013 kb on - strand, within Pf1N1B4_5951 at 400.016 kb on + strand, within Pf1N1B4_5951 at 400.016 kb on + strand, within Pf1N1B4_5951 at 400.016 kb on + strand, within Pf1N1B4_5951 at 400.017 kb on - strand, within Pf1N1B4_5951 at 400.017 kb on - strand, within Pf1N1B4_5951 at 400.017 kb on - strand, within Pf1N1B4_5951 at 400.017 kb on - strand, within Pf1N1B4_5951 at 400.017 kb on - strand, within Pf1N1B4_5951 at 400.017 kb on - strand, within Pf1N1B4_5951 at 400.019 kb on - strand, within Pf1N1B4_5951 at 400.019 kb on - strand, within Pf1N1B4_5951 at 400.028 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 397,774 + +1.1 397,888 + Pf1N1B4_5948 0.11 -0.3 397,888 + Pf1N1B4_5948 0.11 +0.7 397,942 + Pf1N1B4_5948 0.18 +0.5 397,942 + Pf1N1B4_5948 0.18 -0.0 397,942 + Pf1N1B4_5948 0.18 -1.1 397,942 + +0.3 397,942 + Pf1N1B4_5948 0.18 -0.9 397,942 + Pf1N1B4_5948 0.18 -0.0 397,943 - Pf1N1B4_5948 0.18 -0.7 397,943 - Pf1N1B4_5948 0.18 -0.5 397,943 - Pf1N1B4_5948 0.18 -0.9 397,943 - Pf1N1B4_5948 0.18 +1.3 398,101 - Pf1N1B4_5948 0.37 +1.5 398,214 + Pf1N1B4_5948 0.50 -0.9 398,238 - Pf1N1B4_5948 0.53 +0.7 398,528 + Pf1N1B4_5948 0.88 -0.3 398,528 + +0.7 398,528 + Pf1N1B4_5948 0.88 +1.4 398,529 - Pf1N1B4_5948 0.88 -1.7 398,573 + -0.3 398,573 + +0.6 398,574 - -0.0 398,574 - -1.0 398,574 - -1.1 398,679 + -0.0 398,679 + +0.7 398,682 + -2.1 398,702 + -0.9 398,762 + Pf1N1B4_5949 0.14 -0.7 398,763 - Pf1N1B4_5949 0.14 -0.9 398,778 + Pf1N1B4_5949 0.17 -0.6 399,635 + -0.0 399,701 - -0.6 399,796 + +0.1 399,896 - Pf1N1B4_5951 0.12 -1.3 399,899 + Pf1N1B4_5951 0.12 -3.0 399,900 - Pf1N1B4_5951 0.13 -1.7 399,900 - Pf1N1B4_5951 0.13 -3.1 399,900 - Pf1N1B4_5951 0.13 -1.6 399,900 - Pf1N1B4_5951 0.13 -0.5 399,900 - Pf1N1B4_5951 0.13 +0.7 399,900 - Pf1N1B4_5951 0.13 +0.5 399,900 - Pf1N1B4_5951 0.13 -0.7 400,013 - Pf1N1B4_5951 0.26 -0.0 400,016 + Pf1N1B4_5951 0.26 +0.2 400,016 + Pf1N1B4_5951 0.26 +0.4 400,016 + Pf1N1B4_5951 0.26 -0.9 400,017 - Pf1N1B4_5951 0.27 +0.3 400,017 - Pf1N1B4_5951 0.27 -0.9 400,017 - Pf1N1B4_5951 0.27 +1.7 400,017 - Pf1N1B4_5951 0.27 +0.7 400,017 - Pf1N1B4_5951 0.27 -0.5 400,017 - Pf1N1B4_5951 0.27 -0.3 400,019 - Pf1N1B4_5951 0.27 +0.1 400,019 - Pf1N1B4_5951 0.27 -0.5 400,028 - +0.3
Or see this region's nucleotide sequence