Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5835 and Pf1N1B4_5836 are separated by 133 nucleotides Pf1N1B4_5836 and Pf1N1B4_5837 are separated by 110 nucleotides Pf1N1B4_5837 and Pf1N1B4_5838 are separated by 186 nucleotides
Pf1N1B4_5835 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 269,257 to 270,438
_5835
Pf1N1B4_5836 - Putative polyketide synthase (Fragment), at 270,572 to 271,174
_5836
Pf1N1B4_5837 - Transcriptional regulator, LysR family, at 271,285 to 272,181
_5837
Pf1N1B4_5838 - FIG00956589: hypothetical protein, at 272,368 to 273,165
_5838
Position (kb) 271
272
273 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 270.436 kb on + strand at 270.553 kb on + strand at 270.554 kb on - strand at 271.049 kb on + strand, within Pf1N1B4_5836 at 271.049 kb on + strand, within Pf1N1B4_5836 at 271.050 kb on - strand, within Pf1N1B4_5836 at 271.182 kb on - strand at 271.209 kb on - strand at 271.267 kb on + strand at 271.267 kb on + strand at 271.270 kb on + strand at 271.271 kb on - strand at 271.428 kb on - strand, within Pf1N1B4_5837 at 271.604 kb on + strand, within Pf1N1B4_5837 at 271.604 kb on + strand, within Pf1N1B4_5837 at 271.604 kb on + strand at 271.604 kb on + strand, within Pf1N1B4_5837 at 271.604 kb on + strand, within Pf1N1B4_5837 at 271.604 kb on + strand, within Pf1N1B4_5837 at 271.605 kb on - strand, within Pf1N1B4_5837 at 271.605 kb on - strand, within Pf1N1B4_5837 at 271.605 kb on - strand, within Pf1N1B4_5837 at 271.605 kb on - strand, within Pf1N1B4_5837 at 271.699 kb on + strand, within Pf1N1B4_5837 at 271.699 kb on + strand, within Pf1N1B4_5837 at 271.795 kb on + strand, within Pf1N1B4_5837 at 271.796 kb on - strand, within Pf1N1B4_5837 at 272.101 kb on + strand at 272.101 kb on + strand at 272.101 kb on + strand at 272.101 kb on + strand at 272.213 kb on + strand at 272.213 kb on + strand at 272.214 kb on - strand at 272.217 kb on + strand at 272.217 kb on + strand at 272.217 kb on + strand at 272.218 kb on - strand at 272.218 kb on - strand at 272.227 kb on + strand at 272.233 kb on - strand at 272.279 kb on + strand at 272.279 kb on + strand at 272.279 kb on + strand at 272.279 kb on + strand at 272.279 kb on + strand at 272.280 kb on - strand at 272.280 kb on - strand at 272.280 kb on - strand at 272.280 kb on - strand at 272.280 kb on - strand at 272.390 kb on + strand at 272.390 kb on + strand at 272.726 kb on + strand, within Pf1N1B4_5838 at 272.727 kb on - strand, within Pf1N1B4_5838 at 272.903 kb on + strand, within Pf1N1B4_5838 at 272.989 kb on + strand, within Pf1N1B4_5838 at 272.990 kb on - strand, within Pf1N1B4_5838
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 270,436 + +0.3 270,553 + +0.0 270,554 - -2.0 271,049 + Pf1N1B4_5836 0.79 -0.9 271,049 + Pf1N1B4_5836 0.79 +0.3 271,050 - Pf1N1B4_5836 0.79 +1.1 271,182 - -1.6 271,209 - -0.6 271,267 + +1.1 271,267 + -2.1 271,270 + +0.4 271,271 - -0.9 271,428 - Pf1N1B4_5837 0.16 +1.3 271,604 + Pf1N1B4_5837 0.36 -0.6 271,604 + Pf1N1B4_5837 0.36 -0.4 271,604 + -0.6 271,604 + Pf1N1B4_5837 0.36 -1.0 271,604 + Pf1N1B4_5837 0.36 -1.3 271,604 + Pf1N1B4_5837 0.36 -1.6 271,605 - Pf1N1B4_5837 0.36 -1.4 271,605 - Pf1N1B4_5837 0.36 -0.3 271,605 - Pf1N1B4_5837 0.36 -0.1 271,605 - Pf1N1B4_5837 0.36 -1.4 271,699 + Pf1N1B4_5837 0.46 -0.4 271,699 + Pf1N1B4_5837 0.46 -1.9 271,795 + Pf1N1B4_5837 0.57 -0.9 271,796 - Pf1N1B4_5837 0.57 -1.4 272,101 + -0.3 272,101 + -0.2 272,101 + -2.1 272,101 + +3.0 272,213 + +0.3 272,213 + -0.7 272,214 - -1.1 272,217 + -2.3 272,217 + +1.3 272,217 + -0.5 272,218 - +0.1 272,218 - +0.5 272,227 + -1.9 272,233 - -1.6 272,279 + +0.1 272,279 + -0.3 272,279 + -0.6 272,279 + -0.4 272,279 + +1.7 272,280 - -0.9 272,280 - -0.9 272,280 - -0.9 272,280 - -0.0 272,280 - -1.6 272,390 + +0.1 272,390 + +0.3 272,726 + Pf1N1B4_5838 0.45 -1.3 272,727 - Pf1N1B4_5838 0.45 +0.0 272,903 + Pf1N1B4_5838 0.67 +2.0 272,989 + Pf1N1B4_5838 0.78 +0.5 272,990 - Pf1N1B4_5838 0.78 -0.7
Or see this region's nucleotide sequence