Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5782 and Pf1N1B4_5783 are separated by 76 nucleotides Pf1N1B4_5783 and Pf1N1B4_5784 are separated by 22 nucleotides Pf1N1B4_5784 and Pf1N1B4_5785 overlap by 28 nucleotides
Pf1N1B4_5782 - Nitrous oxide reductase maturation protein NosR, at 219,839 to 222,052
_5782
Pf1N1B4_5783 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 222,129 to 223,517
_5783
Pf1N1B4_5784 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 223,540 to 224,085
_5784
Pf1N1B4_5785 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 224,058 to 224,423
_5785
Position (kb) 222
223
224 Strain fitness (log2 ratio) -2
-1
0
1
2 at 221.244 kb on + strand, within Pf1N1B4_5782 at 221.245 kb on - strand at 221.684 kb on + strand, within Pf1N1B4_5782 at 221.684 kb on + strand at 221.684 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.689 kb on - strand, within Pf1N1B4_5782 at 222.056 kb on - strand at 222.493 kb on + strand, within Pf1N1B4_5783 at 222.494 kb on - strand, within Pf1N1B4_5783 at 222.494 kb on - strand, within Pf1N1B4_5783 at 222.649 kb on + strand, within Pf1N1B4_5783 at 222.649 kb on + strand, within Pf1N1B4_5783 at 222.650 kb on - strand, within Pf1N1B4_5783 at 222.650 kb on - strand, within Pf1N1B4_5783 at 222.650 kb on - strand, within Pf1N1B4_5783 at 222.655 kb on + strand, within Pf1N1B4_5783 at 222.776 kb on - strand, within Pf1N1B4_5783 at 222.781 kb on + strand at 223.156 kb on + strand at 223.307 kb on - strand, within Pf1N1B4_5783 at 223.307 kb on - strand, within Pf1N1B4_5783 at 223.309 kb on + strand at 223.309 kb on + strand at 223.342 kb on + strand, within Pf1N1B4_5783 at 223.342 kb on + strand at 223.342 kb on + strand, within Pf1N1B4_5783 at 223.451 kb on + strand at 223.451 kb on + strand at 223.452 kb on - strand at 223.452 kb on - strand at 223.453 kb on + strand at 223.475 kb on - strand at 223.883 kb on + strand, within Pf1N1B4_5784 at 223.883 kb on + strand, within Pf1N1B4_5784 at 223.883 kb on + strand, within Pf1N1B4_5784 at 224.039 kb on + strand at 224.039 kb on + strand at 224.039 kb on + strand at 224.039 kb on + strand at 224.040 kb on - strand at 224.165 kb on - strand, within Pf1N1B4_5785 at 224.165 kb on - strand at 224.335 kb on + strand, within Pf1N1B4_5785 at 224.464 kb on + strand at 224.464 kb on + strand at 224.465 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 221,244 + Pf1N1B4_5782 0.63 -0.6 221,245 - +2.3 221,684 + Pf1N1B4_5782 0.83 -0.6 221,684 + +1.3 221,684 + Pf1N1B4_5782 0.83 -0.3 221,688 + Pf1N1B4_5782 0.84 -1.6 221,688 + Pf1N1B4_5782 0.84 -0.3 221,688 + Pf1N1B4_5782 0.84 -0.9 221,688 + Pf1N1B4_5782 0.84 +1.1 221,688 + Pf1N1B4_5782 0.84 -0.3 221,688 + Pf1N1B4_5782 0.84 +0.4 221,688 + Pf1N1B4_5782 0.84 -0.0 221,688 + Pf1N1B4_5782 0.84 -0.0 221,689 - Pf1N1B4_5782 0.84 -1.9 222,056 - +0.2 222,493 + Pf1N1B4_5783 0.26 -1.6 222,494 - Pf1N1B4_5783 0.26 +0.7 222,494 - Pf1N1B4_5783 0.26 -0.8 222,649 + Pf1N1B4_5783 0.37 +0.3 222,649 + Pf1N1B4_5783 0.37 +1.7 222,650 - Pf1N1B4_5783 0.38 -0.3 222,650 - Pf1N1B4_5783 0.38 +0.4 222,650 - Pf1N1B4_5783 0.38 -0.3 222,655 + Pf1N1B4_5783 0.38 -0.4 222,776 - Pf1N1B4_5783 0.47 +0.0 222,781 + +2.1 223,156 + +0.1 223,307 - Pf1N1B4_5783 0.85 +2.7 223,307 - Pf1N1B4_5783 0.85 -2.1 223,309 + -0.6 223,309 + -1.3 223,342 + Pf1N1B4_5783 0.87 -1.8 223,342 + -0.3 223,342 + Pf1N1B4_5783 0.87 +0.2 223,451 + +1.1 223,451 + +0.5 223,452 - -0.0 223,452 - +0.3 223,453 + +0.0 223,475 - +1.7 223,883 + Pf1N1B4_5784 0.63 -0.3 223,883 + Pf1N1B4_5784 0.63 +1.9 223,883 + Pf1N1B4_5784 0.63 +0.9 224,039 + -0.3 224,039 + -1.3 224,039 + +1.7 224,039 + +0.7 224,040 - -0.3 224,165 - Pf1N1B4_5785 0.29 -1.3 224,165 - +0.4 224,335 + Pf1N1B4_5785 0.76 +0.3 224,464 + -0.5 224,464 + -0.0 224,465 - +0.7
Or see this region's nucleotide sequence