Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5251 and Pf1N1B4_5252 are separated by 169 nucleotides Pf1N1B4_5252 and Pf1N1B4_5253 are separated by 11 nucleotides Pf1N1B4_5253 and Pf1N1B4_5254 are separated by 277 nucleotides
Pf1N1B4_5251 - hypothetical protein, at 9,517 to 10,368
_5251
Pf1N1B4_5252 - hypothetical protein, at 10,538 to 11,272
_5252
Pf1N1B4_5253 - hypothetical protein, at 11,284 to 12,045
_5253
Pf1N1B4_5254 - hypothetical protein, at 12,323 to 13,168
_5254
Position (kb)
11
12
13 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 10.357 kb on - strand at 10.384 kb on + strand at 10.401 kb on - strand at 10.401 kb on - strand at 10.453 kb on - strand at 10.453 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.496 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.500 kb on - strand at 10.552 kb on - strand at 10.733 kb on + strand, within Pf1N1B4_5252 at 10.780 kb on - strand, within Pf1N1B4_5252 at 10.803 kb on + strand, within Pf1N1B4_5252 at 10.803 kb on + strand, within Pf1N1B4_5252 at 10.819 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.821 kb on + strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.822 kb on - strand, within Pf1N1B4_5252 at 10.841 kb on - strand, within Pf1N1B4_5252 at 10.929 kb on + strand, within Pf1N1B4_5252 at 11.130 kb on + strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.131 kb on - strand, within Pf1N1B4_5252 at 11.134 kb on + strand, within Pf1N1B4_5252 at 11.134 kb on + strand, within Pf1N1B4_5252 at 11.159 kb on + strand, within Pf1N1B4_5252 at 11.205 kb on + strand at 11.206 kb on - strand at 11.206 kb on - strand at 11.206 kb on - strand at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.408 kb on + strand, within Pf1N1B4_5253 at 11.409 kb on - strand, within Pf1N1B4_5253 at 11.423 kb on + strand, within Pf1N1B4_5253 at 11.423 kb on + strand, within Pf1N1B4_5253 at 11.424 kb on - strand, within Pf1N1B4_5253 at 11.432 kb on + strand, within Pf1N1B4_5253 at 11.432 kb on + strand at 11.433 kb on - strand, within Pf1N1B4_5253 at 11.790 kb on - strand at 11.790 kb on - strand, within Pf1N1B4_5253 at 11.864 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.920 kb on + strand, within Pf1N1B4_5253 at 11.921 kb on - strand, within Pf1N1B4_5253 at 11.921 kb on - strand, within Pf1N1B4_5253 at 11.924 kb on + strand, within Pf1N1B4_5253 at 11.924 kb on + strand, within Pf1N1B4_5253 at 11.924 kb on + strand, within Pf1N1B4_5253 at 11.925 kb on - strand, within Pf1N1B4_5253 at 11.925 kb on - strand, within Pf1N1B4_5253 at 11.969 kb on + strand at 11.969 kb on + strand at 12.033 kb on + strand at 12.033 kb on + strand at 12.033 kb on + strand at 12.033 kb on + strand at 12.034 kb on - strand at 12.034 kb on - strand at 12.034 kb on - strand at 12.068 kb on - strand at 12.068 kb on - strand at 12.153 kb on + strand at 12.418 kb on - strand, within Pf1N1B4_5254 at 12.485 kb on - strand, within Pf1N1B4_5254 at 12.485 kb on - strand, within Pf1N1B4_5254 at 12.485 kb on - strand, within Pf1N1B4_5254 at 12.546 kb on + strand, within Pf1N1B4_5254 at 12.547 kb on - strand at 12.547 kb on - strand, within Pf1N1B4_5254 at 12.549 kb on + strand, within Pf1N1B4_5254 at 12.549 kb on + strand, within Pf1N1B4_5254 at 12.549 kb on + strand, within Pf1N1B4_5254 at 12.741 kb on + strand, within Pf1N1B4_5254 at 12.859 kb on + strand, within Pf1N1B4_5254 at 12.860 kb on - strand at 12.860 kb on - strand, within Pf1N1B4_5254 at 12.952 kb on + strand, within Pf1N1B4_5254 at 12.953 kb on - strand, within Pf1N1B4_5254
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 10,357 - -0.0 10,384 + +0.6 10,401 - -0.3 10,401 - +2.5 10,453 - +0.8 10,453 - +0.6 10,496 - +1.5 10,496 - +2.0 10,496 - +1.2 10,496 - -1.3 10,500 - -0.3 10,500 - +2.1 10,500 - -1.8 10,500 - -1.5 10,552 - -0.2 10,733 + Pf1N1B4_5252 0.27 -1.9 10,780 - Pf1N1B4_5252 0.33 +1.7 10,803 + Pf1N1B4_5252 0.36 -0.0 10,803 + Pf1N1B4_5252 0.36 +2.6 10,819 + Pf1N1B4_5252 0.38 -0.7 10,821 + Pf1N1B4_5252 0.39 -1.0 10,821 + Pf1N1B4_5252 0.39 -3.3 10,821 + Pf1N1B4_5252 0.39 -0.1 10,822 - Pf1N1B4_5252 0.39 +0.4 10,822 - Pf1N1B4_5252 0.39 -0.7 10,822 - Pf1N1B4_5252 0.39 +0.4 10,841 - Pf1N1B4_5252 0.41 +0.6 10,929 + Pf1N1B4_5252 0.53 +2.0 11,130 + Pf1N1B4_5252 0.81 +0.2 11,131 - Pf1N1B4_5252 0.81 +1.3 11,131 - Pf1N1B4_5252 0.81 +1.0 11,131 - Pf1N1B4_5252 0.81 +1.3 11,134 + Pf1N1B4_5252 0.81 +0.4 11,134 + Pf1N1B4_5252 0.81 +1.0 11,159 + Pf1N1B4_5252 0.84 +0.0 11,205 + -0.0 11,206 - +2.1 11,206 - +0.8 11,206 - -0.3 11,408 + Pf1N1B4_5253 0.16 +1.4 11,408 + Pf1N1B4_5253 0.16 -0.2 11,408 + Pf1N1B4_5253 0.16 +0.2 11,408 + Pf1N1B4_5253 0.16 +0.4 11,409 - Pf1N1B4_5253 0.16 +1.7 11,423 + Pf1N1B4_5253 0.18 -2.3 11,423 + Pf1N1B4_5253 0.18 +2.0 11,424 - Pf1N1B4_5253 0.18 +1.6 11,432 + Pf1N1B4_5253 0.19 -1.2 11,432 + -1.0 11,433 - Pf1N1B4_5253 0.20 -0.6 11,790 - -0.3 11,790 - Pf1N1B4_5253 0.66 -0.6 11,864 + Pf1N1B4_5253 0.76 +0.4 11,920 + Pf1N1B4_5253 0.83 +0.3 11,920 + Pf1N1B4_5253 0.83 +1.0 11,920 + Pf1N1B4_5253 0.83 -2.0 11,920 + Pf1N1B4_5253 0.83 +0.6 11,921 - Pf1N1B4_5253 0.84 +1.3 11,921 - Pf1N1B4_5253 0.84 -0.4 11,924 + Pf1N1B4_5253 0.84 +0.1 11,924 + Pf1N1B4_5253 0.84 +1.0 11,924 + Pf1N1B4_5253 0.84 +0.3 11,925 - Pf1N1B4_5253 0.84 +1.0 11,925 - Pf1N1B4_5253 0.84 -0.9 11,969 + +1.0 11,969 + +0.8 12,033 + -1.3 12,033 + -1.8 12,033 + -0.6 12,033 + +1.0 12,034 - -0.6 12,034 - +0.7 12,034 - +0.4 12,068 - +0.7 12,068 - +1.2 12,153 + -1.3 12,418 - Pf1N1B4_5254 0.11 +1.4 12,485 - Pf1N1B4_5254 0.19 +0.1 12,485 - Pf1N1B4_5254 0.19 -0.6 12,485 - Pf1N1B4_5254 0.19 +0.7 12,546 + Pf1N1B4_5254 0.26 +0.1 12,547 - -0.3 12,547 - Pf1N1B4_5254 0.26 +0.2 12,549 + Pf1N1B4_5254 0.27 -1.3 12,549 + Pf1N1B4_5254 0.27 -1.3 12,549 + Pf1N1B4_5254 0.27 -1.6 12,741 + Pf1N1B4_5254 0.49 +1.0 12,859 + Pf1N1B4_5254 0.63 -0.8 12,860 - +1.0 12,860 - Pf1N1B4_5254 0.63 -0.2 12,952 + Pf1N1B4_5254 0.74 +0.1 12,953 - Pf1N1B4_5254 0.74 -1.1
Or see this region's nucleotide sequence