Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5253

Experiment: nitrate 100 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5251 and Pf1N1B4_5252 are separated by 169 nucleotidesPf1N1B4_5252 and Pf1N1B4_5253 are separated by 11 nucleotidesPf1N1B4_5253 and Pf1N1B4_5254 are separated by 277 nucleotides Pf1N1B4_5251 - hypothetical protein, at 9,517 to 10,368 _5251 Pf1N1B4_5252 - hypothetical protein, at 10,538 to 11,272 _5252 Pf1N1B4_5253 - hypothetical protein, at 11,284 to 12,045 _5253 Pf1N1B4_5254 - hypothetical protein, at 12,323 to 13,168 _5254 Position (kb) 11 12 13Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 10.357 kb on - strandat 10.384 kb on + strandat 10.401 kb on - strandat 10.401 kb on - strandat 10.453 kb on - strandat 10.453 kb on - strandat 10.496 kb on - strandat 10.496 kb on - strandat 10.496 kb on - strandat 10.496 kb on - strandat 10.500 kb on - strandat 10.500 kb on - strandat 10.500 kb on - strandat 10.500 kb on - strandat 10.552 kb on - strandat 10.733 kb on + strand, within Pf1N1B4_5252at 10.780 kb on - strand, within Pf1N1B4_5252at 10.803 kb on + strand, within Pf1N1B4_5252at 10.803 kb on + strand, within Pf1N1B4_5252at 10.819 kb on + strand, within Pf1N1B4_5252at 10.821 kb on + strand, within Pf1N1B4_5252at 10.821 kb on + strand, within Pf1N1B4_5252at 10.821 kb on + strand, within Pf1N1B4_5252at 10.822 kb on - strand, within Pf1N1B4_5252at 10.822 kb on - strand, within Pf1N1B4_5252at 10.822 kb on - strand, within Pf1N1B4_5252at 10.841 kb on - strand, within Pf1N1B4_5252at 10.929 kb on + strand, within Pf1N1B4_5252at 11.130 kb on + strand, within Pf1N1B4_5252at 11.131 kb on - strand, within Pf1N1B4_5252at 11.131 kb on - strand, within Pf1N1B4_5252at 11.131 kb on - strand, within Pf1N1B4_5252at 11.134 kb on + strand, within Pf1N1B4_5252at 11.134 kb on + strand, within Pf1N1B4_5252at 11.159 kb on + strand, within Pf1N1B4_5252at 11.205 kb on + strandat 11.206 kb on - strandat 11.206 kb on - strandat 11.206 kb on - strandat 11.408 kb on + strand, within Pf1N1B4_5253at 11.408 kb on + strand, within Pf1N1B4_5253at 11.408 kb on + strand, within Pf1N1B4_5253at 11.408 kb on + strand, within Pf1N1B4_5253at 11.409 kb on - strand, within Pf1N1B4_5253at 11.423 kb on + strand, within Pf1N1B4_5253at 11.423 kb on + strand, within Pf1N1B4_5253at 11.424 kb on - strand, within Pf1N1B4_5253at 11.432 kb on + strand, within Pf1N1B4_5253at 11.432 kb on + strandat 11.433 kb on - strand, within Pf1N1B4_5253at 11.790 kb on - strandat 11.790 kb on - strand, within Pf1N1B4_5253at 11.864 kb on + strand, within Pf1N1B4_5253at 11.920 kb on + strand, within Pf1N1B4_5253at 11.920 kb on + strand, within Pf1N1B4_5253at 11.920 kb on + strand, within Pf1N1B4_5253at 11.920 kb on + strand, within Pf1N1B4_5253at 11.921 kb on - strand, within Pf1N1B4_5253at 11.921 kb on - strand, within Pf1N1B4_5253at 11.924 kb on + strand, within Pf1N1B4_5253at 11.924 kb on + strand, within Pf1N1B4_5253at 11.924 kb on + strand, within Pf1N1B4_5253at 11.925 kb on - strand, within Pf1N1B4_5253at 11.925 kb on - strand, within Pf1N1B4_5253at 11.969 kb on + strandat 11.969 kb on + strandat 12.033 kb on + strandat 12.033 kb on + strandat 12.033 kb on + strandat 12.033 kb on + strandat 12.034 kb on - strandat 12.034 kb on - strandat 12.034 kb on - strandat 12.068 kb on - strandat 12.068 kb on - strandat 12.153 kb on + strandat 12.418 kb on - strand, within Pf1N1B4_5254at 12.485 kb on - strand, within Pf1N1B4_5254at 12.485 kb on - strand, within Pf1N1B4_5254at 12.485 kb on - strand, within Pf1N1B4_5254at 12.546 kb on + strand, within Pf1N1B4_5254at 12.547 kb on - strandat 12.547 kb on - strand, within Pf1N1B4_5254at 12.549 kb on + strand, within Pf1N1B4_5254at 12.549 kb on + strand, within Pf1N1B4_5254at 12.549 kb on + strand, within Pf1N1B4_5254at 12.741 kb on + strand, within Pf1N1B4_5254at 12.859 kb on + strand, within Pf1N1B4_5254at 12.860 kb on - strandat 12.860 kb on - strand, within Pf1N1B4_5254at 12.952 kb on + strand, within Pf1N1B4_5254at 12.953 kb on - strand, within Pf1N1B4_5254

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 100 mM
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10,357 - -0.0
10,384 + +0.6
10,401 - -0.3
10,401 - +2.5
10,453 - +0.8
10,453 - +0.6
10,496 - +1.5
10,496 - +2.0
10,496 - +1.2
10,496 - -1.3
10,500 - -0.3
10,500 - +2.1
10,500 - -1.8
10,500 - -1.5
10,552 - -0.2
10,733 + Pf1N1B4_5252 0.27 -1.9
10,780 - Pf1N1B4_5252 0.33 +1.7
10,803 + Pf1N1B4_5252 0.36 -0.0
10,803 + Pf1N1B4_5252 0.36 +2.6
10,819 + Pf1N1B4_5252 0.38 -0.7
10,821 + Pf1N1B4_5252 0.39 -1.0
10,821 + Pf1N1B4_5252 0.39 -3.3
10,821 + Pf1N1B4_5252 0.39 -0.1
10,822 - Pf1N1B4_5252 0.39 +0.4
10,822 - Pf1N1B4_5252 0.39 -0.7
10,822 - Pf1N1B4_5252 0.39 +0.4
10,841 - Pf1N1B4_5252 0.41 +0.6
10,929 + Pf1N1B4_5252 0.53 +2.0
11,130 + Pf1N1B4_5252 0.81 +0.2
11,131 - Pf1N1B4_5252 0.81 +1.3
11,131 - Pf1N1B4_5252 0.81 +1.0
11,131 - Pf1N1B4_5252 0.81 +1.3
11,134 + Pf1N1B4_5252 0.81 +0.4
11,134 + Pf1N1B4_5252 0.81 +1.0
11,159 + Pf1N1B4_5252 0.84 +0.0
11,205 + -0.0
11,206 - +2.1
11,206 - +0.8
11,206 - -0.3
11,408 + Pf1N1B4_5253 0.16 +1.4
11,408 + Pf1N1B4_5253 0.16 -0.2
11,408 + Pf1N1B4_5253 0.16 +0.2
11,408 + Pf1N1B4_5253 0.16 +0.4
11,409 - Pf1N1B4_5253 0.16 +1.7
11,423 + Pf1N1B4_5253 0.18 -2.3
11,423 + Pf1N1B4_5253 0.18 +2.0
11,424 - Pf1N1B4_5253 0.18 +1.6
11,432 + Pf1N1B4_5253 0.19 -1.2
11,432 + -1.0
11,433 - Pf1N1B4_5253 0.20 -0.6
11,790 - -0.3
11,790 - Pf1N1B4_5253 0.66 -0.6
11,864 + Pf1N1B4_5253 0.76 +0.4
11,920 + Pf1N1B4_5253 0.83 +0.3
11,920 + Pf1N1B4_5253 0.83 +1.0
11,920 + Pf1N1B4_5253 0.83 -2.0
11,920 + Pf1N1B4_5253 0.83 +0.6
11,921 - Pf1N1B4_5253 0.84 +1.3
11,921 - Pf1N1B4_5253 0.84 -0.4
11,924 + Pf1N1B4_5253 0.84 +0.1
11,924 + Pf1N1B4_5253 0.84 +1.0
11,924 + Pf1N1B4_5253 0.84 +0.3
11,925 - Pf1N1B4_5253 0.84 +1.0
11,925 - Pf1N1B4_5253 0.84 -0.9
11,969 + +1.0
11,969 + +0.8
12,033 + -1.3
12,033 + -1.8
12,033 + -0.6
12,033 + +1.0
12,034 - -0.6
12,034 - +0.7
12,034 - +0.4
12,068 - +0.7
12,068 - +1.2
12,153 + -1.3
12,418 - Pf1N1B4_5254 0.11 +1.4
12,485 - Pf1N1B4_5254 0.19 +0.1
12,485 - Pf1N1B4_5254 0.19 -0.6
12,485 - Pf1N1B4_5254 0.19 +0.7
12,546 + Pf1N1B4_5254 0.26 +0.1
12,547 - -0.3
12,547 - Pf1N1B4_5254 0.26 +0.2
12,549 + Pf1N1B4_5254 0.27 -1.3
12,549 + Pf1N1B4_5254 0.27 -1.3
12,549 + Pf1N1B4_5254 0.27 -1.6
12,741 + Pf1N1B4_5254 0.49 +1.0
12,859 + Pf1N1B4_5254 0.63 -0.8
12,860 - +1.0
12,860 - Pf1N1B4_5254 0.63 -0.2
12,952 + Pf1N1B4_5254 0.74 +0.1
12,953 - Pf1N1B4_5254 0.74 -1.1

Or see this region's nucleotide sequence