Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_516

Experiment: nitrate 100 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_515 and Pf1N1B4_516 overlap by 1 nucleotidesPf1N1B4_516 and Pf1N1B4_517 are separated by 80 nucleotidesPf1N1B4_517 and Pf1N1B4_518 are separated by 12 nucleotides Pf1N1B4_515 - DNA-binding response regulator GltR, controls specific porins for the entry of glucose, at 606,483 to 607,241 _515 Pf1N1B4_516 - Sensor histidine kinase, at 607,241 to 608,293 _516 Pf1N1B4_517 - hypothetical protein, at 608,374 to 608,517 _517 Pf1N1B4_518 - FKBP-type peptidyl-prolyl cis-trans isomerase FkpA precursor (EC 5.2.1.8), at 608,530 to 609,378 _518 Position (kb) 607 608 609Strain fitness (log2 ratio) -2 -1 0 1 2at 606.294 kb on + strandat 606.294 kb on + strandat 606.295 kb on - strandat 606.415 kb on + strandat 606.416 kb on - strandat 606.441 kb on + strandat 606.441 kb on + strandat 606.441 kb on + strandat 606.442 kb on - strandat 606.461 kb on + strandat 606.461 kb on + strandat 606.461 kb on + strandat 606.536 kb on + strandat 606.604 kb on - strand, within Pf1N1B4_515at 606.621 kb on - strand, within Pf1N1B4_515at 606.837 kb on + strand, within Pf1N1B4_515at 606.837 kb on + strand, within Pf1N1B4_515at 606.837 kb on + strand, within Pf1N1B4_515at 606.837 kb on + strand, within Pf1N1B4_515at 606.838 kb on - strand, within Pf1N1B4_515at 606.838 kb on - strand, within Pf1N1B4_515at 606.838 kb on - strandat 606.838 kb on - strand, within Pf1N1B4_515at 606.838 kb on - strand, within Pf1N1B4_515at 606.955 kb on + strandat 607.213 kb on - strandat 607.213 kb on - strandat 607.258 kb on + strandat 607.302 kb on + strandat 607.302 kb on + strandat 607.303 kb on - strandat 607.832 kb on + strand, within Pf1N1B4_516at 607.832 kb on + strand, within Pf1N1B4_516at 607.832 kb on + strandat 607.832 kb on + strand, within Pf1N1B4_516at 607.833 kb on - strandat 607.833 kb on - strand, within Pf1N1B4_516at 608.158 kb on + strand, within Pf1N1B4_516at 608.158 kb on + strand, within Pf1N1B4_516at 608.407 kb on - strand, within Pf1N1B4_517at 608.455 kb on - strand, within Pf1N1B4_517at 608.486 kb on - strandat 608.486 kb on - strand, within Pf1N1B4_517at 608.486 kb on - strand, within Pf1N1B4_517at 608.486 kb on - strandat 608.554 kb on + strandat 608.554 kb on + strandat 608.555 kb on - strandat 608.555 kb on - strandat 608.563 kb on + strandat 608.563 kb on + strandat 608.563 kb on + strandat 608.564 kb on - strandat 608.564 kb on - strandat 608.564 kb on - strandat 608.564 kb on - strandat 608.564 kb on - strandat 608.565 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 100 mM
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606,294 + +2.0
606,294 + -0.0
606,295 - -0.8
606,415 + +0.4
606,416 - +0.4
606,441 + -2.2
606,441 + +0.2
606,441 + +1.0
606,442 - +0.7
606,461 + +1.3
606,461 + +0.7
606,461 + +1.4
606,536 + -0.6
606,604 - Pf1N1B4_515 0.16 -1.0
606,621 - Pf1N1B4_515 0.18 +1.0
606,837 + Pf1N1B4_515 0.47 -0.0
606,837 + Pf1N1B4_515 0.47 +0.2
606,837 + Pf1N1B4_515 0.47 +0.2
606,837 + Pf1N1B4_515 0.47 -0.8
606,838 - Pf1N1B4_515 0.47 -1.4
606,838 - Pf1N1B4_515 0.47 -1.4
606,838 - +1.0
606,838 - Pf1N1B4_515 0.47 -0.4
606,838 - Pf1N1B4_515 0.47 +0.7
606,955 + -0.0
607,213 - +0.1
607,213 - +0.2
607,258 + +1.0
607,302 + -1.0
607,302 + +2.0
607,303 - -0.8
607,832 + Pf1N1B4_516 0.56 +0.2
607,832 + Pf1N1B4_516 0.56 -1.2
607,832 + -0.6
607,832 + Pf1N1B4_516 0.56 -0.8
607,833 - -0.0
607,833 - Pf1N1B4_516 0.56 -1.2
608,158 + Pf1N1B4_516 0.87 -1.8
608,158 + Pf1N1B4_516 0.87 +0.5
608,407 - Pf1N1B4_517 0.23 -1.2
608,455 - Pf1N1B4_517 0.56 -0.6
608,486 - +1.5
608,486 - Pf1N1B4_517 0.78 +1.0
608,486 - Pf1N1B4_517 0.78 +0.4
608,486 - -0.0
608,554 + +1.0
608,554 + -0.0
608,555 - +0.5
608,555 - +0.5
608,563 + -0.5
608,563 + +2.0
608,563 + -1.6
608,564 - +0.5
608,564 - -1.4
608,564 - -1.7
608,564 - -0.0
608,564 - -2.0
608,565 + -0.6

Or see this region's nucleotide sequence