Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5000

Experiment: nitrate 100 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4999 and Pf1N1B4_5000 are separated by 169 nucleotidesPf1N1B4_5000 and Pf1N1B4_5001 are separated by 331 nucleotides Pf1N1B4_4999 - Sugar diacid utilization regulator SdaR, at 5,548,023 to 5,549,153 _4999 Pf1N1B4_5000 - D-glycerate transporter (predicted), at 5,549,323 to 5,550,633 _5000 Pf1N1B4_5001 - Oxidoreductase, aldo/keto reductase family, at 5,550,965 to 5,551,978 _5001 Position (kb) 5549 5550 5551Strain fitness (log2 ratio) -2 -1 0 1 2at 5548.470 kb on - strand, within Pf1N1B4_4999at 5548.849 kb on + strand, within Pf1N1B4_4999at 5548.849 kb on + strandat 5548.849 kb on + strand, within Pf1N1B4_4999at 5548.849 kb on + strandat 5548.850 kb on - strand, within Pf1N1B4_4999at 5549.304 kb on - strandat 5549.304 kb on - strandat 5549.434 kb on + strandat 5549.543 kb on + strand, within Pf1N1B4_5000at 5549.543 kb on + strand, within Pf1N1B4_5000at 5549.543 kb on + strand, within Pf1N1B4_5000at 5549.543 kb on + strand, within Pf1N1B4_5000at 5549.544 kb on - strand, within Pf1N1B4_5000at 5549.544 kb on - strand, within Pf1N1B4_5000at 5549.935 kb on + strand, within Pf1N1B4_5000at 5549.956 kb on + strand, within Pf1N1B4_5000at 5550.234 kb on + strand, within Pf1N1B4_5000at 5550.234 kb on + strand, within Pf1N1B4_5000at 5550.235 kb on - strandat 5550.235 kb on - strand, within Pf1N1B4_5000at 5550.235 kb on - strand, within Pf1N1B4_5000at 5550.563 kb on - strandat 5550.563 kb on - strandat 5550.632 kb on - strandat 5550.632 kb on - strandat 5550.672 kb on + strandat 5550.687 kb on + strandat 5550.687 kb on + strandat 5550.687 kb on + strandat 5550.688 kb on - strandat 5550.688 kb on - strandat 5550.688 kb on - strandat 5550.688 kb on - strandat 5550.696 kb on + strandat 5550.925 kb on + strandat 5550.926 kb on - strandat 5551.222 kb on + strand, within Pf1N1B4_5001at 5551.313 kb on + strand, within Pf1N1B4_5001at 5551.313 kb on + strand, within Pf1N1B4_5001at 5551.313 kb on + strand, within Pf1N1B4_5001at 5551.314 kb on - strand, within Pf1N1B4_5001at 5551.314 kb on - strand, within Pf1N1B4_5001at 5551.316 kb on + strand, within Pf1N1B4_5001at 5551.317 kb on - strand, within Pf1N1B4_5001at 5551.499 kb on + strand, within Pf1N1B4_5001at 5551.500 kb on - strand, within Pf1N1B4_5001at 5551.575 kb on - strand, within Pf1N1B4_5001at 5551.610 kb on + strand, within Pf1N1B4_5001

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 100 mM
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5,548,470 - Pf1N1B4_4999 0.40 -0.8
5,548,849 + Pf1N1B4_4999 0.73 +0.2
5,548,849 + +1.1
5,548,849 + Pf1N1B4_4999 0.73 -0.4
5,548,849 + +0.6
5,548,850 - Pf1N1B4_4999 0.73 -0.4
5,549,304 - +2.1
5,549,304 - -0.8
5,549,434 + -0.3
5,549,543 + Pf1N1B4_5000 0.17 +0.6
5,549,543 + Pf1N1B4_5000 0.17 -0.4
5,549,543 + Pf1N1B4_5000 0.17 -0.4
5,549,543 + Pf1N1B4_5000 0.17 -1.4
5,549,544 - Pf1N1B4_5000 0.17 -0.4
5,549,544 - Pf1N1B4_5000 0.17 +0.1
5,549,935 + Pf1N1B4_5000 0.47 +0.1
5,549,956 + Pf1N1B4_5000 0.48 -2.2
5,550,234 + Pf1N1B4_5000 0.69 -1.4
5,550,234 + Pf1N1B4_5000 0.69 -1.0
5,550,235 - +0.6
5,550,235 - Pf1N1B4_5000 0.70 -2.0
5,550,235 - Pf1N1B4_5000 0.70 -1.4
5,550,563 - +0.6
5,550,563 - -0.7
5,550,632 - -0.2
5,550,632 - +0.7
5,550,672 + -0.9
5,550,687 + -0.0
5,550,687 + +1.0
5,550,687 + +1.9
5,550,688 - -2.2
5,550,688 - +0.2
5,550,688 - +0.8
5,550,688 - +1.3
5,550,696 + -0.2
5,550,925 + -0.1
5,550,926 - -1.1
5,551,222 + Pf1N1B4_5001 0.25 +1.1
5,551,313 + Pf1N1B4_5001 0.34 -0.2
5,551,313 + Pf1N1B4_5001 0.34 -0.0
5,551,313 + Pf1N1B4_5001 0.34 +0.9
5,551,314 - Pf1N1B4_5001 0.34 +2.6
5,551,314 - Pf1N1B4_5001 0.34 -0.6
5,551,316 + Pf1N1B4_5001 0.35 -0.2
5,551,317 - Pf1N1B4_5001 0.35 +0.6
5,551,499 + Pf1N1B4_5001 0.53 -0.9
5,551,500 - Pf1N1B4_5001 0.53 -0.4
5,551,575 - Pf1N1B4_5001 0.60 -1.4
5,551,610 + Pf1N1B4_5001 0.64 -1.4

Or see this region's nucleotide sequence