Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4999 and Pf1N1B4_5000 are separated by 169 nucleotides Pf1N1B4_5000 and Pf1N1B4_5001 are separated by 331 nucleotides
Pf1N1B4_4999 - Sugar diacid utilization regulator SdaR, at 5,548,023 to 5,549,153
_4999
Pf1N1B4_5000 - D-glycerate transporter (predicted), at 5,549,323 to 5,550,633
_5000
Pf1N1B4_5001 - Oxidoreductase, aldo/keto reductase family, at 5,550,965 to 5,551,978
_5001
Position (kb)
5549
5550
5551 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5548.470 kb on - strand, within Pf1N1B4_4999 at 5548.849 kb on + strand, within Pf1N1B4_4999 at 5548.849 kb on + strand at 5548.849 kb on + strand, within Pf1N1B4_4999 at 5548.849 kb on + strand at 5548.850 kb on - strand, within Pf1N1B4_4999 at 5549.304 kb on - strand at 5549.304 kb on - strand at 5549.434 kb on + strand at 5549.543 kb on + strand, within Pf1N1B4_5000 at 5549.543 kb on + strand, within Pf1N1B4_5000 at 5549.543 kb on + strand, within Pf1N1B4_5000 at 5549.543 kb on + strand, within Pf1N1B4_5000 at 5549.544 kb on - strand, within Pf1N1B4_5000 at 5549.544 kb on - strand, within Pf1N1B4_5000 at 5549.935 kb on + strand, within Pf1N1B4_5000 at 5549.956 kb on + strand, within Pf1N1B4_5000 at 5550.234 kb on + strand, within Pf1N1B4_5000 at 5550.234 kb on + strand, within Pf1N1B4_5000 at 5550.235 kb on - strand at 5550.235 kb on - strand, within Pf1N1B4_5000 at 5550.235 kb on - strand, within Pf1N1B4_5000 at 5550.563 kb on - strand at 5550.563 kb on - strand at 5550.632 kb on - strand at 5550.632 kb on - strand at 5550.672 kb on + strand at 5550.687 kb on + strand at 5550.687 kb on + strand at 5550.687 kb on + strand at 5550.688 kb on - strand at 5550.688 kb on - strand at 5550.688 kb on - strand at 5550.688 kb on - strand at 5550.696 kb on + strand at 5550.925 kb on + strand at 5550.926 kb on - strand at 5551.222 kb on + strand, within Pf1N1B4_5001 at 5551.313 kb on + strand, within Pf1N1B4_5001 at 5551.313 kb on + strand, within Pf1N1B4_5001 at 5551.313 kb on + strand, within Pf1N1B4_5001 at 5551.314 kb on - strand, within Pf1N1B4_5001 at 5551.314 kb on - strand, within Pf1N1B4_5001 at 5551.316 kb on + strand, within Pf1N1B4_5001 at 5551.317 kb on - strand, within Pf1N1B4_5001 at 5551.499 kb on + strand, within Pf1N1B4_5001 at 5551.500 kb on - strand, within Pf1N1B4_5001 at 5551.575 kb on - strand, within Pf1N1B4_5001 at 5551.610 kb on + strand, within Pf1N1B4_5001
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 5,548,470 - Pf1N1B4_4999 0.40 -0.8 5,548,849 + Pf1N1B4_4999 0.73 +0.2 5,548,849 + +1.1 5,548,849 + Pf1N1B4_4999 0.73 -0.4 5,548,849 + +0.6 5,548,850 - Pf1N1B4_4999 0.73 -0.4 5,549,304 - +2.1 5,549,304 - -0.8 5,549,434 + -0.3 5,549,543 + Pf1N1B4_5000 0.17 +0.6 5,549,543 + Pf1N1B4_5000 0.17 -0.4 5,549,543 + Pf1N1B4_5000 0.17 -0.4 5,549,543 + Pf1N1B4_5000 0.17 -1.4 5,549,544 - Pf1N1B4_5000 0.17 -0.4 5,549,544 - Pf1N1B4_5000 0.17 +0.1 5,549,935 + Pf1N1B4_5000 0.47 +0.1 5,549,956 + Pf1N1B4_5000 0.48 -2.2 5,550,234 + Pf1N1B4_5000 0.69 -1.4 5,550,234 + Pf1N1B4_5000 0.69 -1.0 5,550,235 - +0.6 5,550,235 - Pf1N1B4_5000 0.70 -2.0 5,550,235 - Pf1N1B4_5000 0.70 -1.4 5,550,563 - +0.6 5,550,563 - -0.7 5,550,632 - -0.2 5,550,632 - +0.7 5,550,672 + -0.9 5,550,687 + -0.0 5,550,687 + +1.0 5,550,687 + +1.9 5,550,688 - -2.2 5,550,688 - +0.2 5,550,688 - +0.8 5,550,688 - +1.3 5,550,696 + -0.2 5,550,925 + -0.1 5,550,926 - -1.1 5,551,222 + Pf1N1B4_5001 0.25 +1.1 5,551,313 + Pf1N1B4_5001 0.34 -0.2 5,551,313 + Pf1N1B4_5001 0.34 -0.0 5,551,313 + Pf1N1B4_5001 0.34 +0.9 5,551,314 - Pf1N1B4_5001 0.34 +2.6 5,551,314 - Pf1N1B4_5001 0.34 -0.6 5,551,316 + Pf1N1B4_5001 0.35 -0.2 5,551,317 - Pf1N1B4_5001 0.35 +0.6 5,551,499 + Pf1N1B4_5001 0.53 -0.9 5,551,500 - Pf1N1B4_5001 0.53 -0.4 5,551,575 - Pf1N1B4_5001 0.60 -1.4 5,551,610 + Pf1N1B4_5001 0.64 -1.4
Or see this region's nucleotide sequence