Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_480 and Pf1N1B4_481 are separated by 100 nucleotides Pf1N1B4_481 and Pf1N1B4_482 are separated by 196 nucleotides Pf1N1B4_482 and Pf1N1B4_483 are separated by 135 nucleotides Pf1N1B4_483 and Pf1N1B4_484 are separated by 92 nucleotides
Pf1N1B4_480 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 570,930 to 573,119
_480
Pf1N1B4_481 - FIG00956241: hypothetical protein, at 573,220 to 573,627
_481
Pf1N1B4_482 - Transcriptional regulator, LysR family, at 573,824 to 574,822
_482
Pf1N1B4_483 - Metal-dependent hydrolase related to alanyl-tRNA synthetase, at 574,958 to 575,539
_483
Pf1N1B4_484 - Hydrogenase maturation factor, at 575,632 to 576,402
_484
Position (kb) 573
574
575 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 573.095 kb on - strand at 573.153 kb on + strand at 573.153 kb on + strand at 573.153 kb on + strand at 573.154 kb on - strand at 573.154 kb on - strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.156 kb on + strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.157 kb on - strand at 573.181 kb on + strand at 573.181 kb on + strand at 573.181 kb on + strand at 573.182 kb on - strand at 573.182 kb on - strand at 573.183 kb on + strand at 573.183 kb on + strand at 573.183 kb on + strand at 573.184 kb on - strand at 573.184 kb on - strand at 573.184 kb on - strand at 573.184 kb on - strand at 573.184 kb on - strand at 573.214 kb on + strand at 573.214 kb on + strand at 573.214 kb on + strand at 573.214 kb on + strand at 573.215 kb on - strand at 573.215 kb on - strand at 573.215 kb on - strand at 573.215 kb on - strand at 573.284 kb on + strand, within Pf1N1B4_481 at 573.285 kb on - strand, within Pf1N1B4_481 at 573.285 kb on - strand, within Pf1N1B4_481 at 573.285 kb on - strand, within Pf1N1B4_481 at 573.386 kb on - strand, within Pf1N1B4_481 at 573.466 kb on + strand, within Pf1N1B4_481 at 573.466 kb on + strand, within Pf1N1B4_481 at 573.466 kb on + strand at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.490 kb on + strand, within Pf1N1B4_481 at 573.491 kb on - strand at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.491 kb on - strand, within Pf1N1B4_481 at 573.517 kb on + strand, within Pf1N1B4_481 at 573.593 kb on + strand at 573.662 kb on + strand at 573.663 kb on - strand at 573.855 kb on + strand at 573.855 kb on + strand at 573.855 kb on + strand at 573.856 kb on - strand at 573.856 kb on - strand at 573.937 kb on - strand, within Pf1N1B4_482 at 574.083 kb on + strand, within Pf1N1B4_482 at 574.233 kb on + strand, within Pf1N1B4_482 at 574.233 kb on + strand, within Pf1N1B4_482 at 574.234 kb on - strand, within Pf1N1B4_482 at 574.234 kb on - strand, within Pf1N1B4_482 at 574.237 kb on + strand, within Pf1N1B4_482 at 574.237 kb on + strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.238 kb on - strand, within Pf1N1B4_482 at 574.313 kb on + strand, within Pf1N1B4_482 at 574.313 kb on + strand, within Pf1N1B4_482 at 574.314 kb on - strand, within Pf1N1B4_482 at 574.314 kb on - strand, within Pf1N1B4_482 at 574.314 kb on - strand, within Pf1N1B4_482 at 574.727 kb on + strand at 574.727 kb on + strand at 574.728 kb on - strand at 574.728 kb on - strand at 574.728 kb on - strand at 574.882 kb on - strand at 574.888 kb on - strand at 575.451 kb on + strand, within Pf1N1B4_483 at 575.451 kb on + strand, within Pf1N1B4_483 at 575.451 kb on + strand, within Pf1N1B4_483 at 575.451 kb on + strand, within Pf1N1B4_483 at 575.452 kb on - strand, within Pf1N1B4_483 at 575.526 kb on - strand at 575.526 kb on - strand at 575.526 kb on - strand at 575.526 kb on - strand at 575.615 kb on + strand at 575.615 kb on + strand at 575.615 kb on + strand at 575.625 kb on + strand at 575.625 kb on + strand at 575.625 kb on + strand at 575.626 kb on - strand at 575.669 kb on + strand at 575.669 kb on + strand at 575.669 kb on + strand at 575.670 kb on - strand at 575.779 kb on + strand, within Pf1N1B4_484 at 575.779 kb on + strand, within Pf1N1B4_484 at 575.779 kb on + strand, within Pf1N1B4_484 at 575.779 kb on + strand, within Pf1N1B4_484 at 575.779 kb on + strand, within Pf1N1B4_484 at 575.780 kb on - strand, within Pf1N1B4_484 at 575.780 kb on - strand, within Pf1N1B4_484 at 575.780 kb on - strand, within Pf1N1B4_484 at 575.805 kb on + strand, within Pf1N1B4_484 at 575.805 kb on + strand, within Pf1N1B4_484 at 575.821 kb on + strand, within Pf1N1B4_484 at 575.821 kb on + strand, within Pf1N1B4_484 at 575.821 kb on + strand, within Pf1N1B4_484 at 575.822 kb on - strand, within Pf1N1B4_484
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 573,095 - +0.4 573,153 + +0.1 573,153 + +0.7 573,153 + +1.2 573,154 - -0.6 573,154 - -2.5 573,156 + -0.0 573,156 + -0.3 573,156 + +2.3 573,156 + -1.0 573,156 + -1.2 573,156 + +3.0 573,157 - -0.8 573,157 - +0.7 573,157 - -1.0 573,157 - +1.0 573,157 - -0.6 573,157 - -1.6 573,157 - +1.0 573,181 + +1.4 573,181 + -0.1 573,181 + +1.0 573,182 - -0.1 573,182 - +0.2 573,183 + +1.3 573,183 + -0.8 573,183 + -0.0 573,184 - +1.4 573,184 - -0.6 573,184 - +1.0 573,184 - +1.0 573,184 - -1.0 573,214 + -1.0 573,214 + +0.7 573,214 + +1.0 573,214 + -0.8 573,215 - -2.6 573,215 - -0.8 573,215 - -1.8 573,215 - -0.1 573,284 + Pf1N1B4_481 0.16 -1.3 573,285 - Pf1N1B4_481 0.16 +0.4 573,285 - Pf1N1B4_481 0.16 +1.0 573,285 - Pf1N1B4_481 0.16 +0.2 573,386 - Pf1N1B4_481 0.41 -2.3 573,466 + Pf1N1B4_481 0.60 -1.3 573,466 + Pf1N1B4_481 0.60 -0.4 573,466 + -0.6 573,490 + Pf1N1B4_481 0.66 +0.1 573,490 + Pf1N1B4_481 0.66 -0.3 573,490 + Pf1N1B4_481 0.66 +1.0 573,490 + Pf1N1B4_481 0.66 -1.2 573,490 + Pf1N1B4_481 0.66 -0.3 573,490 + Pf1N1B4_481 0.66 +0.2 573,490 + Pf1N1B4_481 0.66 -0.8 573,490 + Pf1N1B4_481 0.66 +0.4 573,491 - -0.6 573,491 - Pf1N1B4_481 0.66 +1.0 573,491 - Pf1N1B4_481 0.66 +0.4 573,491 - Pf1N1B4_481 0.66 -1.2 573,491 - Pf1N1B4_481 0.66 -1.0 573,491 - Pf1N1B4_481 0.66 -0.3 573,491 - Pf1N1B4_481 0.66 -0.9 573,517 + Pf1N1B4_481 0.73 +1.6 573,593 + +0.5 573,662 + +0.1 573,663 - -0.0 573,855 + -0.3 573,855 + +1.0 573,855 + +1.0 573,856 - +1.4 573,856 - -1.0 573,937 - Pf1N1B4_482 0.11 -1.6 574,083 + Pf1N1B4_482 0.26 -0.6 574,233 + Pf1N1B4_482 0.41 +2.0 574,233 + Pf1N1B4_482 0.41 +1.4 574,234 - Pf1N1B4_482 0.41 -0.6 574,234 - Pf1N1B4_482 0.41 +0.7 574,237 + Pf1N1B4_482 0.41 +1.0 574,237 + Pf1N1B4_482 0.41 -3.3 574,238 - Pf1N1B4_482 0.41 -1.3 574,238 - Pf1N1B4_482 0.41 -0.3 574,238 - Pf1N1B4_482 0.41 +1.0 574,238 - Pf1N1B4_482 0.41 -0.2 574,238 - Pf1N1B4_482 0.41 -0.4 574,238 - Pf1N1B4_482 0.41 +0.3 574,238 - Pf1N1B4_482 0.41 +1.6 574,238 - Pf1N1B4_482 0.41 +3.3 574,313 + Pf1N1B4_482 0.49 -0.4 574,313 + Pf1N1B4_482 0.49 +0.6 574,314 - Pf1N1B4_482 0.49 +1.9 574,314 - Pf1N1B4_482 0.49 -0.4 574,314 - Pf1N1B4_482 0.49 +1.0 574,727 + +1.2 574,727 + +1.8 574,728 - +0.2 574,728 - +0.7 574,728 - +1.4 574,882 - +0.2 574,888 - -1.2 575,451 + Pf1N1B4_483 0.85 -0.4 575,451 + Pf1N1B4_483 0.85 +0.2 575,451 + Pf1N1B4_483 0.85 +0.4 575,451 + Pf1N1B4_483 0.85 -1.8 575,452 - Pf1N1B4_483 0.85 -0.3 575,526 - +0.6 575,526 - +1.0 575,526 - -0.0 575,526 - +0.2 575,615 + -0.6 575,615 + -1.3 575,615 + -0.4 575,625 + +0.3 575,625 + -0.3 575,625 + +0.5 575,626 - +0.2 575,669 + -0.0 575,669 + +0.7 575,669 + +0.4 575,670 - -0.0 575,779 + Pf1N1B4_484 0.19 -0.9 575,779 + Pf1N1B4_484 0.19 -0.8 575,779 + Pf1N1B4_484 0.19 -0.6 575,779 + Pf1N1B4_484 0.19 -1.6 575,779 + Pf1N1B4_484 0.19 +0.7 575,780 - Pf1N1B4_484 0.19 +0.1 575,780 - Pf1N1B4_484 0.19 -1.0 575,780 - Pf1N1B4_484 0.19 -0.6 575,805 + Pf1N1B4_484 0.22 +0.6 575,805 + Pf1N1B4_484 0.22 -2.2 575,821 + Pf1N1B4_484 0.25 -0.2 575,821 + Pf1N1B4_484 0.25 -0.6 575,821 + Pf1N1B4_484 0.25 -1.8 575,822 - Pf1N1B4_484 0.25 -0.4
Or see this region's nucleotide sequence