Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4699 and Pf1N1B4_4700 are separated by 591 nucleotides Pf1N1B4_4700 and Pf1N1B4_4701 are separated by 19 nucleotides Pf1N1B4_4701 and Pf1N1B4_4702 are separated by 234 nucleotides
Pf1N1B4_4699 - Multidrug translocase MdfA, at 5,192,584 to 5,193,825
_4699
Pf1N1B4_4700 - FIG00956898: hypothetical protein, at 5,194,417 to 5,194,605
_4700
Pf1N1B4_4701 - Fe2+/Zn2+ uptake regulation proteins, at 5,194,625 to 5,194,996
_4701
Pf1N1B4_4702 - Acyl-homoserine lactone acylase PvdQ (EC 3.5.1.-), quorum-quenching, at 5,195,231 to 5,197,576
_4702
Position (kb)
5194
5195 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5193.651 kb on - strand, within Pf1N1B4_4699 at 5193.743 kb on + strand at 5193.744 kb on - strand at 5193.802 kb on + strand at 5193.803 kb on - strand at 5193.803 kb on - strand at 5193.804 kb on + strand at 5193.805 kb on - strand at 5193.809 kb on - strand at 5194.049 kb on + strand at 5194.050 kb on - strand at 5194.094 kb on + strand at 5194.116 kb on + strand at 5194.534 kb on + strand, within Pf1N1B4_4700 at 5194.534 kb on + strand, within Pf1N1B4_4700 at 5194.992 kb on + strand at 5194.992 kb on + strand at 5194.993 kb on - strand at 5194.993 kb on - strand at 5194.993 kb on - strand at 5195.076 kb on + strand at 5195.076 kb on + strand at 5195.077 kb on - strand at 5195.089 kb on + strand at 5195.094 kb on - strand at 5195.148 kb on + strand at 5195.149 kb on - strand at 5195.206 kb on + strand at 5195.414 kb on + strand at 5195.415 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 5,193,651 - Pf1N1B4_4699 0.86 -0.9 5,193,743 + +1.0 5,193,744 - -0.6 5,193,802 + -1.5 5,193,803 - +2.0 5,193,803 - -1.5 5,193,804 + -1.5 5,193,805 - -0.9 5,193,809 - +0.2 5,194,049 + -0.3 5,194,050 - -0.0 5,194,094 + +0.1 5,194,116 + +0.1 5,194,534 + Pf1N1B4_4700 0.62 -1.8 5,194,534 + Pf1N1B4_4700 0.62 -1.2 5,194,992 + -0.2 5,194,992 + -0.2 5,194,993 - +0.8 5,194,993 - -0.5 5,194,993 - -1.0 5,195,076 + +3.5 5,195,076 + +0.4 5,195,077 - -0.1 5,195,089 + -0.5 5,195,094 - +0.2 5,195,148 + +2.1 5,195,149 - +0.5 5,195,206 + -0.2 5,195,414 + +2.5 5,195,415 - -1.0
Or see this region's nucleotide sequence