Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4656 and Pf1N1B4_4657 overlap by 4 nucleotides Pf1N1B4_4657 and Pf1N1B4_4658 are separated by 227 nucleotides
Pf1N1B4_4656 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 5,133,850 to 5,135,523
_4656
Pf1N1B4_4657 - Oxidoreductase probably involved in sulfite reduction, at 5,135,520 to 5,136,014
_4657
Pf1N1B4_4658 - hypothetical protein, at 5,136,242 to 5,137,210
_4658
Position (kb) 5135
5136
5137 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5134.558 kb on + strand, within Pf1N1B4_4656 at 5134.559 kb on - strand, within Pf1N1B4_4656 at 5134.588 kb on + strand, within Pf1N1B4_4656 at 5135.183 kb on - strand, within Pf1N1B4_4656 at 5135.215 kb on - strand, within Pf1N1B4_4656 at 5135.451 kb on + strand at 5135.452 kb on - strand at 5135.787 kb on + strand, within Pf1N1B4_4657 at 5135.799 kb on + strand, within Pf1N1B4_4657 at 5135.854 kb on + strand, within Pf1N1B4_4657 at 5135.854 kb on + strand, within Pf1N1B4_4657 at 5135.854 kb on + strand, within Pf1N1B4_4657 at 5135.854 kb on + strand, within Pf1N1B4_4657 at 5135.855 kb on - strand, within Pf1N1B4_4657 at 5135.919 kb on - strand, within Pf1N1B4_4657 at 5135.919 kb on - strand, within Pf1N1B4_4657 at 5136.213 kb on - strand at 5136.315 kb on - strand at 5136.366 kb on - strand, within Pf1N1B4_4658 at 5136.631 kb on + strand, within Pf1N1B4_4658 at 5136.707 kb on + strand, within Pf1N1B4_4658 at 5136.734 kb on + strand, within Pf1N1B4_4658 at 5136.734 kb on + strand, within Pf1N1B4_4658 at 5136.735 kb on - strand, within Pf1N1B4_4658 at 5136.737 kb on + strand, within Pf1N1B4_4658 at 5136.737 kb on + strand, within Pf1N1B4_4658 at 5136.737 kb on + strand, within Pf1N1B4_4658 at 5136.759 kb on + strand, within Pf1N1B4_4658 at 5136.827 kb on + strand, within Pf1N1B4_4658 at 5136.842 kb on + strand, within Pf1N1B4_4658 at 5136.843 kb on - strand at 5136.881 kb on + strand, within Pf1N1B4_4658 at 5136.882 kb on - strand, within Pf1N1B4_4658
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 5,134,558 + Pf1N1B4_4656 0.42 -1.4 5,134,559 - Pf1N1B4_4656 0.42 +0.0 5,134,588 + Pf1N1B4_4656 0.44 -0.4 5,135,183 - Pf1N1B4_4656 0.80 +0.2 5,135,215 - Pf1N1B4_4656 0.82 -0.9 5,135,451 + +0.2 5,135,452 - +1.4 5,135,787 + Pf1N1B4_4657 0.54 -2.5 5,135,799 + Pf1N1B4_4657 0.56 +0.1 5,135,854 + Pf1N1B4_4657 0.67 -1.5 5,135,854 + Pf1N1B4_4657 0.67 -0.5 5,135,854 + Pf1N1B4_4657 0.67 -1.0 5,135,854 + Pf1N1B4_4657 0.67 -0.5 5,135,855 - Pf1N1B4_4657 0.68 -1.3 5,135,919 - Pf1N1B4_4657 0.81 +0.9 5,135,919 - Pf1N1B4_4657 0.81 -2.0 5,136,213 - -0.5 5,136,315 - +0.9 5,136,366 - Pf1N1B4_4658 0.13 -3.4 5,136,631 + Pf1N1B4_4658 0.40 -0.5 5,136,707 + Pf1N1B4_4658 0.48 -2.5 5,136,734 + Pf1N1B4_4658 0.51 -0.5 5,136,734 + Pf1N1B4_4658 0.51 -1.8 5,136,735 - Pf1N1B4_4658 0.51 +0.3 5,136,737 + Pf1N1B4_4658 0.51 +2.9 5,136,737 + Pf1N1B4_4658 0.51 -0.9 5,136,737 + Pf1N1B4_4658 0.51 +2.9 5,136,759 + Pf1N1B4_4658 0.53 -0.5 5,136,827 + Pf1N1B4_4658 0.60 -1.5 5,136,842 + Pf1N1B4_4658 0.62 -0.5 5,136,843 - -0.7 5,136,881 + Pf1N1B4_4658 0.66 +0.4 5,136,882 - Pf1N1B4_4658 0.66 +2.1
Or see this region's nucleotide sequence