Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4066 and Pf1N1B4_4067 are separated by 90 nucleotides Pf1N1B4_4067 and Pf1N1B4_4068 are separated by 73 nucleotides Pf1N1B4_4068 and Pf1N1B4_4069 overlap by 4 nucleotides Pf1N1B4_4069 and Pf1N1B4_4070 overlap by 1 nucleotides Pf1N1B4_4070 and Pf1N1B4_4071 are separated by 1 nucleotides
Pf1N1B4_4066 - Glutathione S-transferase, omega (EC 2.5.1.18), at 4,520,824 to 4,521,828
_4066
Pf1N1B4_4067 - Anthranilate phosphoribosyltransferase like (EC 2.4.2.18), at 4,521,919 to 4,522,920
_4067
Pf1N1B4_4068 - tRNA 2-thiouridine synthesizing protein E (EC 2.8.1.-), at 4,522,994 to 4,523,251
_4068
Pf1N1B4_4069 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusB, at 4,523,248 to 4,523,541
_4069
Pf1N1B4_4070 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusC, at 4,523,541 to 4,523,903
_4070
Pf1N1B4_4071 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusD, at 4,523,905 to 4,524,327
_4071
Position (kb)
4521
4522
4523 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4520.949 kb on - strand, within Pf1N1B4_4066 at 4520.949 kb on - strand, within Pf1N1B4_4066 at 4520.951 kb on + strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4521.006 kb on - strand, within Pf1N1B4_4066 at 4521.006 kb on - strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.081 kb on - strand, within Pf1N1B4_4066 at 4521.200 kb on - strand at 4521.221 kb on + strand, within Pf1N1B4_4066 at 4521.222 kb on - strand, within Pf1N1B4_4066 at 4521.222 kb on - strand, within Pf1N1B4_4066 at 4521.479 kb on + strand, within Pf1N1B4_4066 at 4521.662 kb on + strand at 4521.663 kb on - strand, within Pf1N1B4_4066 at 4521.788 kb on + strand at 4521.817 kb on + strand at 4521.868 kb on + strand at 4521.911 kb on - strand at 4522.041 kb on + strand at 4522.041 kb on + strand, within Pf1N1B4_4067 at 4522.041 kb on + strand, within Pf1N1B4_4067 at 4522.042 kb on - strand, within Pf1N1B4_4067 at 4522.042 kb on - strand, within Pf1N1B4_4067 at 4522.167 kb on - strand, within Pf1N1B4_4067 at 4522.167 kb on - strand, within Pf1N1B4_4067 at 4522.628 kb on + strand, within Pf1N1B4_4067 at 4522.629 kb on - strand, within Pf1N1B4_4067 at 4522.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 4,520,949 - Pf1N1B4_4066 0.12 -1.1 4,520,949 - Pf1N1B4_4066 0.12 +0.3 4,520,951 + Pf1N1B4_4066 0.13 +0.0 4,520,952 - Pf1N1B4_4066 0.13 +2.2 4,520,952 - Pf1N1B4_4066 0.13 -0.1 4,520,952 - Pf1N1B4_4066 0.13 +0.7 4,520,952 - Pf1N1B4_4066 0.13 -0.6 4,521,006 - Pf1N1B4_4066 0.18 -1.8 4,521,006 - Pf1N1B4_4066 0.18 -2.0 4,521,080 + Pf1N1B4_4066 0.25 -0.6 4,521,080 + Pf1N1B4_4066 0.25 +0.9 4,521,080 + Pf1N1B4_4066 0.25 -1.1 4,521,081 - Pf1N1B4_4066 0.26 -0.8 4,521,200 - -0.8 4,521,221 + Pf1N1B4_4066 0.40 -0.4 4,521,222 - Pf1N1B4_4066 0.40 -1.1 4,521,222 - Pf1N1B4_4066 0.40 +1.1 4,521,479 + Pf1N1B4_4066 0.65 -0.3 4,521,662 + +1.7 4,521,663 - Pf1N1B4_4066 0.83 -0.4 4,521,788 + +0.7 4,521,817 + +0.3 4,521,868 + +0.1 4,521,911 - +0.2 4,522,041 + +0.8 4,522,041 + Pf1N1B4_4067 0.12 -0.6 4,522,041 + Pf1N1B4_4067 0.12 +0.8 4,522,042 - Pf1N1B4_4067 0.12 -0.7 4,522,042 - Pf1N1B4_4067 0.12 -2.1 4,522,167 - Pf1N1B4_4067 0.25 -0.5 4,522,167 - Pf1N1B4_4067 0.25 -0.7 4,522,628 + Pf1N1B4_4067 0.71 -1.1 4,522,629 - Pf1N1B4_4067 0.71 -0.9 4,522,911 - +0.8
Or see this region's nucleotide sequence