Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3868

Experiment: nitrate 100 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3866 and Pf1N1B4_3867 are separated by 79 nucleotidesPf1N1B4_3867 and Pf1N1B4_3868 are separated by 93 nucleotidesPf1N1B4_3868 and Pf1N1B4_3869 are separated by 214 nucleotidesPf1N1B4_3869 and Pf1N1B4_3870 overlap by 8 nucleotides Pf1N1B4_3866 - Adenine phosphoribosyltransferase (EC 2.4.2.7), at 4,295,915 to 4,296,463 _3866 Pf1N1B4_3867 - Fumarate and nitrate reduction regulatory protein, at 4,296,543 to 4,297,277 _3867 Pf1N1B4_3868 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 4,297,371 to 4,298,753 _3868 Pf1N1B4_3869 - Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein, at 4,298,968 to 4,299,651 _3869 Pf1N1B4_3870 - Type cbb3 cytochrome oxidase biogenesis protein CcoS, involved in heme b insertion, at 4,299,644 to 4,299,862 _3870 Position (kb) 4297 4298 4299Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4296.499 kb on + strandat 4296.499 kb on + strandat 4296.596 kb on + strandat 4296.597 kb on - strandat 4296.600 kb on + strandat 4296.600 kb on + strandat 4296.601 kb on - strandat 4296.685 kb on + strand, within Pf1N1B4_3867at 4296.686 kb on - strand, within Pf1N1B4_3867at 4297.307 kb on - strandat 4297.373 kb on - strandat 4297.373 kb on - strandat 4297.373 kb on - strandat 4297.373 kb on - strandat 4297.468 kb on - strandat 4297.546 kb on + strand, within Pf1N1B4_3868at 4297.547 kb on - strand, within Pf1N1B4_3868at 4297.735 kb on + strand, within Pf1N1B4_3868at 4297.817 kb on + strand, within Pf1N1B4_3868at 4297.817 kb on + strand, within Pf1N1B4_3868at 4297.817 kb on + strand, within Pf1N1B4_3868at 4297.817 kb on + strand, within Pf1N1B4_3868at 4297.818 kb on - strand, within Pf1N1B4_3868at 4297.818 kb on - strand, within Pf1N1B4_3868at 4297.962 kb on + strand, within Pf1N1B4_3868at 4298.017 kb on + strand, within Pf1N1B4_3868at 4298.017 kb on + strand, within Pf1N1B4_3868at 4298.017 kb on + strand, within Pf1N1B4_3868at 4298.017 kb on + strand, within Pf1N1B4_3868at 4298.107 kb on + strand, within Pf1N1B4_3868at 4298.108 kb on - strand, within Pf1N1B4_3868at 4298.497 kb on + strand, within Pf1N1B4_3868at 4298.552 kb on - strand, within Pf1N1B4_3868at 4298.584 kb on + strand, within Pf1N1B4_3868at 4298.584 kb on + strand, within Pf1N1B4_3868at 4298.584 kb on + strand, within Pf1N1B4_3868at 4298.584 kb on + strand, within Pf1N1B4_3868at 4298.584 kb on + strand, within Pf1N1B4_3868at 4298.584 kb on + strand, within Pf1N1B4_3868at 4298.584 kb on + strand, within Pf1N1B4_3868at 4298.585 kb on - strand, within Pf1N1B4_3868at 4298.610 kb on - strand, within Pf1N1B4_3868at 4298.687 kb on + strandat 4298.687 kb on + strandat 4298.687 kb on + strandat 4298.688 kb on - strandat 4298.688 kb on - strandat 4298.969 kb on + strandat 4298.969 kb on + strandat 4298.970 kb on - strandat 4299.070 kb on + strand, within Pf1N1B4_3869at 4299.071 kb on - strand, within Pf1N1B4_3869at 4299.200 kb on + strand, within Pf1N1B4_3869at 4299.200 kb on + strand, within Pf1N1B4_3869at 4299.200 kb on + strand, within Pf1N1B4_3869at 4299.200 kb on + strand, within Pf1N1B4_3869at 4299.201 kb on - strand, within Pf1N1B4_3869at 4299.201 kb on - strand, within Pf1N1B4_3869at 4299.272 kb on - strand, within Pf1N1B4_3869at 4299.272 kb on - strand, within Pf1N1B4_3869at 4299.272 kb on - strand, within Pf1N1B4_3869at 4299.272 kb on - strand, within Pf1N1B4_3869at 4299.272 kb on - strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.274 kb on + strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.275 kb on - strand, within Pf1N1B4_3869at 4299.362 kb on + strand, within Pf1N1B4_3869at 4299.362 kb on + strand, within Pf1N1B4_3869at 4299.362 kb on + strand, within Pf1N1B4_3869at 4299.362 kb on + strand, within Pf1N1B4_3869at 4299.362 kb on + strand, within Pf1N1B4_3869at 4299.362 kb on + strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.363 kb on - strand, within Pf1N1B4_3869at 4299.473 kb on + strand, within Pf1N1B4_3869at 4299.473 kb on + strand, within Pf1N1B4_3869at 4299.474 kb on - strand, within Pf1N1B4_3869at 4299.474 kb on - strand, within Pf1N1B4_3869at 4299.474 kb on - strand, within Pf1N1B4_3869at 4299.495 kb on + strand, within Pf1N1B4_3869at 4299.495 kb on + strand, within Pf1N1B4_3869at 4299.495 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.497 kb on + strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.498 kb on - strand, within Pf1N1B4_3869at 4299.508 kb on + strand, within Pf1N1B4_3869at 4299.509 kb on - strand, within Pf1N1B4_3869at 4299.509 kb on - strand, within Pf1N1B4_3869at 4299.509 kb on - strand, within Pf1N1B4_3869at 4299.509 kb on - strand, within Pf1N1B4_3869

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 100 mM
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4,296,499 + -0.2
4,296,499 + -0.5
4,296,596 + -1.7
4,296,597 - -1.5
4,296,600 + -1.2
4,296,600 + -0.8
4,296,601 - +1.5
4,296,685 + Pf1N1B4_3867 0.19 -1.3
4,296,686 - Pf1N1B4_3867 0.19 -2.2
4,297,307 - +0.8
4,297,373 - +1.0
4,297,373 - -0.2
4,297,373 - +0.3
4,297,373 - -0.7
4,297,468 - -0.6
4,297,546 + Pf1N1B4_3868 0.13 +0.4
4,297,547 - Pf1N1B4_3868 0.13 -0.6
4,297,735 + Pf1N1B4_3868 0.26 +0.4
4,297,817 + Pf1N1B4_3868 0.32 +0.5
4,297,817 + Pf1N1B4_3868 0.32 +0.8
4,297,817 + Pf1N1B4_3868 0.32 +1.1
4,297,817 + Pf1N1B4_3868 0.32 -0.6
4,297,818 - Pf1N1B4_3868 0.32 -0.8
4,297,818 - Pf1N1B4_3868 0.32 -0.2
4,297,962 + Pf1N1B4_3868 0.43 +0.4
4,298,017 + Pf1N1B4_3868 0.47 -0.2
4,298,017 + Pf1N1B4_3868 0.47 -1.2
4,298,017 + Pf1N1B4_3868 0.47 -0.2
4,298,017 + Pf1N1B4_3868 0.47 -0.2
4,298,107 + Pf1N1B4_3868 0.53 +0.2
4,298,108 - Pf1N1B4_3868 0.53 +0.4
4,298,497 + Pf1N1B4_3868 0.81 -0.6
4,298,552 - Pf1N1B4_3868 0.85 -0.8
4,298,584 + Pf1N1B4_3868 0.88 -1.7
4,298,584 + Pf1N1B4_3868 0.88 -2.4
4,298,584 + Pf1N1B4_3868 0.88 +0.0
4,298,584 + Pf1N1B4_3868 0.88 -0.1
4,298,584 + Pf1N1B4_3868 0.88 +1.5
4,298,584 + Pf1N1B4_3868 0.88 -1.2
4,298,584 + Pf1N1B4_3868 0.88 +0.8
4,298,585 - Pf1N1B4_3868 0.88 -0.6
4,298,610 - Pf1N1B4_3868 0.90 +1.4
4,298,687 + -1.6
4,298,687 + -3.0
4,298,687 + -0.2
4,298,688 - +0.2
4,298,688 - +0.4
4,298,969 + -2.6
4,298,969 + +0.4
4,298,970 - -0.0
4,299,070 + Pf1N1B4_3869 0.15 -2.0
4,299,071 - Pf1N1B4_3869 0.15 +0.4
4,299,200 + Pf1N1B4_3869 0.34 +2.1
4,299,200 + Pf1N1B4_3869 0.34 +0.5
4,299,200 + Pf1N1B4_3869 0.34 +0.8
4,299,200 + Pf1N1B4_3869 0.34 -0.0
4,299,201 - Pf1N1B4_3869 0.34 -0.2
4,299,201 - Pf1N1B4_3869 0.34 +1.8
4,299,272 - Pf1N1B4_3869 0.44 -0.6
4,299,272 - Pf1N1B4_3869 0.44 +1.8
4,299,272 - Pf1N1B4_3869 0.44 +0.2
4,299,272 - Pf1N1B4_3869 0.44 +0.0
4,299,272 - Pf1N1B4_3869 0.44 -1.1
4,299,274 + Pf1N1B4_3869 0.45 +0.4
4,299,274 + Pf1N1B4_3869 0.45 -1.6
4,299,274 + Pf1N1B4_3869 0.45 -0.5
4,299,274 + Pf1N1B4_3869 0.45 -0.3
4,299,274 + Pf1N1B4_3869 0.45 +0.0
4,299,274 + Pf1N1B4_3869 0.45 -1.6
4,299,274 + Pf1N1B4_3869 0.45 -1.0
4,299,274 + Pf1N1B4_3869 0.45 +0.6
4,299,274 + Pf1N1B4_3869 0.45 +1.8
4,299,274 + Pf1N1B4_3869 0.45 +1.1
4,299,274 + Pf1N1B4_3869 0.45 +0.8
4,299,274 + Pf1N1B4_3869 0.45 -0.8
4,299,274 + Pf1N1B4_3869 0.45 -1.0
4,299,274 + Pf1N1B4_3869 0.45 +0.0
4,299,274 + Pf1N1B4_3869 0.45 -0.4
4,299,274 + Pf1N1B4_3869 0.45 -0.2
4,299,275 - Pf1N1B4_3869 0.45 +0.3
4,299,275 - Pf1N1B4_3869 0.45 +1.4
4,299,275 - Pf1N1B4_3869 0.45 -1.8
4,299,275 - Pf1N1B4_3869 0.45 +1.3
4,299,275 - Pf1N1B4_3869 0.45 -1.2
4,299,275 - Pf1N1B4_3869 0.45 -0.6
4,299,275 - Pf1N1B4_3869 0.45 +0.4
4,299,275 - Pf1N1B4_3869 0.45 -1.6
4,299,275 - Pf1N1B4_3869 0.45 -0.2
4,299,275 - Pf1N1B4_3869 0.45 -2.2
4,299,275 - Pf1N1B4_3869 0.45 +1.5
4,299,275 - Pf1N1B4_3869 0.45 -2.6
4,299,275 - Pf1N1B4_3869 0.45 -0.7
4,299,275 - Pf1N1B4_3869 0.45 +0.1
4,299,275 - Pf1N1B4_3869 0.45 +0.5
4,299,275 - Pf1N1B4_3869 0.45 -0.7
4,299,275 - Pf1N1B4_3869 0.45 +0.2
4,299,275 - Pf1N1B4_3869 0.45 +0.8
4,299,275 - Pf1N1B4_3869 0.45 -0.8
4,299,275 - Pf1N1B4_3869 0.45 -0.3
4,299,275 - Pf1N1B4_3869 0.45 -0.5
4,299,275 - Pf1N1B4_3869 0.45 -0.4
4,299,275 - Pf1N1B4_3869 0.45 +0.4
4,299,275 - Pf1N1B4_3869 0.45 -0.5
4,299,275 - Pf1N1B4_3869 0.45 -0.1
4,299,275 - Pf1N1B4_3869 0.45 +0.3
4,299,362 + Pf1N1B4_3869 0.58 -1.0
4,299,362 + Pf1N1B4_3869 0.58 +0.0
4,299,362 + Pf1N1B4_3869 0.58 -0.4
4,299,362 + Pf1N1B4_3869 0.58 -0.1
4,299,362 + Pf1N1B4_3869 0.58 -1.2
4,299,362 + Pf1N1B4_3869 0.58 +0.2
4,299,363 - Pf1N1B4_3869 0.58 +0.8
4,299,363 - Pf1N1B4_3869 0.58 -0.8
4,299,363 - Pf1N1B4_3869 0.58 -1.0
4,299,363 - Pf1N1B4_3869 0.58 +1.4
4,299,363 - Pf1N1B4_3869 0.58 -0.1
4,299,363 - Pf1N1B4_3869 0.58 +1.5
4,299,363 - Pf1N1B4_3869 0.58 -1.2
4,299,363 - Pf1N1B4_3869 0.58 +0.5
4,299,363 - Pf1N1B4_3869 0.58 -0.7
4,299,363 - Pf1N1B4_3869 0.58 -0.5
4,299,363 - Pf1N1B4_3869 0.58 +0.2
4,299,363 - Pf1N1B4_3869 0.58 -0.8
4,299,363 - Pf1N1B4_3869 0.58 -0.8
4,299,473 + Pf1N1B4_3869 0.74 -0.8
4,299,473 + Pf1N1B4_3869 0.74 -0.2
4,299,474 - Pf1N1B4_3869 0.74 +0.5
4,299,474 - Pf1N1B4_3869 0.74 -0.0
4,299,474 - Pf1N1B4_3869 0.74 +0.3
4,299,495 + Pf1N1B4_3869 0.77 +0.1
4,299,495 + Pf1N1B4_3869 0.77 +0.1
4,299,495 + Pf1N1B4_3869 0.77 +0.0
4,299,497 + Pf1N1B4_3869 0.77 -0.6
4,299,497 + Pf1N1B4_3869 0.77 +0.4
4,299,497 + Pf1N1B4_3869 0.77 +0.8
4,299,497 + Pf1N1B4_3869 0.77 +1.1
4,299,497 + Pf1N1B4_3869 0.77 +0.5
4,299,497 + Pf1N1B4_3869 0.77 -0.8
4,299,497 + Pf1N1B4_3869 0.77 +0.4
4,299,497 + Pf1N1B4_3869 0.77 +0.4
4,299,497 + Pf1N1B4_3869 0.77 +0.4
4,299,497 + Pf1N1B4_3869 0.77 +1.2
4,299,497 + Pf1N1B4_3869 0.77 -0.8
4,299,497 + Pf1N1B4_3869 0.77 +0.8
4,299,497 + Pf1N1B4_3869 0.77 +0.5
4,299,497 + Pf1N1B4_3869 0.77 +1.2
4,299,498 - Pf1N1B4_3869 0.77 -0.8
4,299,498 - Pf1N1B4_3869 0.77 -0.0
4,299,498 - Pf1N1B4_3869 0.77 +1.4
4,299,498 - Pf1N1B4_3869 0.77 +0.8
4,299,498 - Pf1N1B4_3869 0.77 +1.8
4,299,498 - Pf1N1B4_3869 0.77 +0.9
4,299,498 - Pf1N1B4_3869 0.77 -1.8
4,299,498 - Pf1N1B4_3869 0.77 -0.2
4,299,498 - Pf1N1B4_3869 0.77 -0.1
4,299,498 - Pf1N1B4_3869 0.77 -1.7
4,299,498 - Pf1N1B4_3869 0.77 -0.0
4,299,498 - Pf1N1B4_3869 0.77 -0.6
4,299,498 - Pf1N1B4_3869 0.77 +0.8
4,299,498 - Pf1N1B4_3869 0.77 +0.5
4,299,508 + Pf1N1B4_3869 0.79 -0.4
4,299,509 - Pf1N1B4_3869 0.79 +1.0
4,299,509 - Pf1N1B4_3869 0.79 -0.4
4,299,509 - Pf1N1B4_3869 0.79 -0.3
4,299,509 - Pf1N1B4_3869 0.79 -0.1

Or see this region's nucleotide sequence