Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3416 and Pf1N1B4_3417 are separated by 359 nucleotides Pf1N1B4_3417 and Pf1N1B4_3418 are separated by 138 nucleotides Pf1N1B4_3418 and Pf1N1B4_3419 are separated by 23 nucleotides Pf1N1B4_3419 and Pf1N1B4_3420 are separated by 130 nucleotides
Pf1N1B4_3416 - Outer membrane protein romA, at 3,805,873 to 3,806,931
_3416
Pf1N1B4_3417 - FIG00964635: hypothetical protein, at 3,807,291 to 3,807,608
_3417
Pf1N1B4_3418 - hypothetical protein, at 3,807,747 to 3,807,860
_3418
Pf1N1B4_3419 - Periplasmic beta-glucosidase (EC 3.2.1.21), at 3,807,884 to 3,810,175
_3419
Pf1N1B4_3420 - Transcriptional regulator, AraC family, at 3,810,306 to 3,811,073
_3420
Position (kb)
3807
3808
3809
3810
3811 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3806.983 kb on + strand at 3806.984 kb on - strand at 3806.984 kb on - strand at 3806.984 kb on - strand at 3806.999 kb on + strand at 3807.000 kb on - strand at 3807.026 kb on + strand at 3807.026 kb on + strand at 3807.027 kb on - strand at 3807.027 kb on - strand at 3807.027 kb on - strand at 3807.085 kb on + strand at 3807.085 kb on + strand at 3807.097 kb on + strand at 3807.098 kb on - strand at 3807.098 kb on - strand at 3807.189 kb on + strand at 3807.307 kb on + strand at 3807.308 kb on - strand at 3807.749 kb on - strand at 3807.894 kb on + strand at 3807.894 kb on + strand at 3807.894 kb on + strand at 3807.903 kb on + strand at 3807.903 kb on + strand at 3807.903 kb on + strand at 3807.903 kb on + strand at 3807.904 kb on - strand at 3807.904 kb on - strand at 3807.987 kb on + strand at 3808.065 kb on + strand at 3808.103 kb on + strand at 3808.104 kb on - strand at 3808.197 kb on + strand, within Pf1N1B4_3419 at 3808.335 kb on + strand, within Pf1N1B4_3419 at 3808.335 kb on + strand, within Pf1N1B4_3419 at 3808.335 kb on + strand at 3808.335 kb on + strand, within Pf1N1B4_3419 at 3808.335 kb on + strand, within Pf1N1B4_3419 at 3808.335 kb on + strand, within Pf1N1B4_3419 at 3808.336 kb on - strand, within Pf1N1B4_3419 at 3808.336 kb on - strand, within Pf1N1B4_3419 at 3808.336 kb on - strand, within Pf1N1B4_3419 at 3808.336 kb on - strand, within Pf1N1B4_3419 at 3808.336 kb on - strand, within Pf1N1B4_3419 at 3808.394 kb on + strand, within Pf1N1B4_3419 at 3808.395 kb on - strand, within Pf1N1B4_3419 at 3808.496 kb on + strand, within Pf1N1B4_3419 at 3808.496 kb on + strand, within Pf1N1B4_3419 at 3808.496 kb on + strand, within Pf1N1B4_3419 at 3808.496 kb on + strand, within Pf1N1B4_3419 at 3808.497 kb on - strand, within Pf1N1B4_3419 at 3808.497 kb on - strand, within Pf1N1B4_3419 at 3808.523 kb on + strand, within Pf1N1B4_3419 at 3808.523 kb on + strand, within Pf1N1B4_3419 at 3808.565 kb on + strand, within Pf1N1B4_3419 at 3808.577 kb on + strand, within Pf1N1B4_3419 at 3808.633 kb on - strand, within Pf1N1B4_3419 at 3808.807 kb on + strand, within Pf1N1B4_3419 at 3808.829 kb on + strand, within Pf1N1B4_3419 at 3808.830 kb on - strand, within Pf1N1B4_3419 at 3808.843 kb on - strand, within Pf1N1B4_3419 at 3808.843 kb on - strand, within Pf1N1B4_3419 at 3808.919 kb on + strand, within Pf1N1B4_3419 at 3808.919 kb on + strand, within Pf1N1B4_3419 at 3808.920 kb on - strand, within Pf1N1B4_3419 at 3809.028 kb on + strand, within Pf1N1B4_3419 at 3809.028 kb on + strand, within Pf1N1B4_3419 at 3809.106 kb on + strand, within Pf1N1B4_3419 at 3809.107 kb on - strand at 3809.107 kb on - strand, within Pf1N1B4_3419 at 3809.187 kb on - strand, within Pf1N1B4_3419 at 3809.228 kb on + strand, within Pf1N1B4_3419 at 3809.228 kb on + strand, within Pf1N1B4_3419 at 3809.228 kb on + strand, within Pf1N1B4_3419 at 3809.228 kb on + strand, within Pf1N1B4_3419 at 3809.228 kb on + strand, within Pf1N1B4_3419 at 3809.229 kb on - strand, within Pf1N1B4_3419 at 3809.229 kb on - strand, within Pf1N1B4_3419 at 3809.229 kb on - strand, within Pf1N1B4_3419 at 3809.270 kb on - strand, within Pf1N1B4_3419 at 3809.273 kb on + strand, within Pf1N1B4_3419 at 3809.385 kb on + strand, within Pf1N1B4_3419 at 3809.573 kb on + strand, within Pf1N1B4_3419 at 3809.574 kb on - strand, within Pf1N1B4_3419 at 3809.574 kb on - strand, within Pf1N1B4_3419 at 3809.625 kb on - strand, within Pf1N1B4_3419 at 3809.644 kb on - strand at 3809.684 kb on + strand, within Pf1N1B4_3419 at 3809.684 kb on + strand at 3809.684 kb on + strand, within Pf1N1B4_3419 at 3809.684 kb on + strand, within Pf1N1B4_3419 at 3809.684 kb on + strand, within Pf1N1B4_3419 at 3809.684 kb on + strand, within Pf1N1B4_3419 at 3809.685 kb on - strand, within Pf1N1B4_3419 at 3809.685 kb on - strand, within Pf1N1B4_3419 at 3809.685 kb on - strand, within Pf1N1B4_3419 at 3809.687 kb on + strand, within Pf1N1B4_3419 at 3809.714 kb on + strand, within Pf1N1B4_3419 at 3809.715 kb on - strand, within Pf1N1B4_3419 at 3809.718 kb on + strand, within Pf1N1B4_3419 at 3809.718 kb on + strand, within Pf1N1B4_3419 at 3809.718 kb on + strand at 3809.719 kb on - strand, within Pf1N1B4_3419 at 3809.719 kb on - strand, within Pf1N1B4_3419 at 3809.719 kb on - strand, within Pf1N1B4_3419 at 3809.738 kb on + strand, within Pf1N1B4_3419 at 3809.738 kb on + strand, within Pf1N1B4_3419 at 3809.738 kb on + strand, within Pf1N1B4_3419 at 3809.738 kb on + strand, within Pf1N1B4_3419 at 3809.739 kb on - strand, within Pf1N1B4_3419 at 3809.751 kb on - strand, within Pf1N1B4_3419 at 3809.801 kb on + strand, within Pf1N1B4_3419 at 3809.802 kb on - strand, within Pf1N1B4_3419 at 3809.802 kb on - strand, within Pf1N1B4_3419 at 3809.802 kb on - strand, within Pf1N1B4_3419 at 3809.925 kb on + strand, within Pf1N1B4_3419 at 3809.925 kb on + strand, within Pf1N1B4_3419 at 3809.925 kb on + strand, within Pf1N1B4_3419 at 3809.925 kb on + strand, within Pf1N1B4_3419 at 3809.925 kb on + strand, within Pf1N1B4_3419 at 3809.926 kb on - strand, within Pf1N1B4_3419 at 3809.926 kb on - strand, within Pf1N1B4_3419 at 3809.926 kb on - strand, within Pf1N1B4_3419 at 3809.927 kb on + strand, within Pf1N1B4_3419 at 3809.927 kb on + strand, within Pf1N1B4_3419 at 3809.927 kb on + strand, within Pf1N1B4_3419 at 3809.927 kb on + strand, within Pf1N1B4_3419 at 3809.927 kb on + strand at 3809.927 kb on + strand, within Pf1N1B4_3419 at 3809.927 kb on + strand, within Pf1N1B4_3419 at 3809.927 kb on + strand, within Pf1N1B4_3419 at 3809.927 kb on + strand, within Pf1N1B4_3419 at 3809.927 kb on + strand, within Pf1N1B4_3419 at 3809.927 kb on + strand, within Pf1N1B4_3419 at 3809.927 kb on + strand, within Pf1N1B4_3419 at 3809.928 kb on - strand, within Pf1N1B4_3419 at 3809.928 kb on - strand, within Pf1N1B4_3419 at 3809.928 kb on - strand at 3809.928 kb on - strand at 3809.928 kb on - strand, within Pf1N1B4_3419 at 3809.928 kb on - strand, within Pf1N1B4_3419 at 3809.928 kb on - strand, within Pf1N1B4_3419 at 3809.928 kb on - strand, within Pf1N1B4_3419 at 3809.928 kb on - strand, within Pf1N1B4_3419 at 3809.929 kb on + strand, within Pf1N1B4_3419 at 3809.929 kb on + strand, within Pf1N1B4_3419 at 3809.929 kb on + strand, within Pf1N1B4_3419 at 3809.930 kb on - strand, within Pf1N1B4_3419 at 3809.930 kb on - strand, within Pf1N1B4_3419 at 3810.058 kb on + strand at 3810.195 kb on - strand at 3810.243 kb on - strand at 3810.308 kb on - strand at 3810.308 kb on - strand at 3810.308 kb on - strand at 3810.522 kb on - strand, within Pf1N1B4_3420 at 3810.534 kb on - strand, within Pf1N1B4_3420 at 3810.564 kb on + strand, within Pf1N1B4_3420 at 3810.565 kb on - strand, within Pf1N1B4_3420 at 3810.657 kb on - strand, within Pf1N1B4_3420 at 3810.715 kb on + strand, within Pf1N1B4_3420 at 3810.820 kb on + strand, within Pf1N1B4_3420 at 3810.820 kb on + strand, within Pf1N1B4_3420 at 3810.820 kb on + strand, within Pf1N1B4_3420 at 3810.821 kb on - strand, within Pf1N1B4_3420 at 3810.821 kb on - strand, within Pf1N1B4_3420 at 3810.821 kb on - strand, within Pf1N1B4_3420 at 3810.821 kb on - strand, within Pf1N1B4_3420 at 3810.821 kb on - strand, within Pf1N1B4_3420 at 3810.923 kb on + strand, within Pf1N1B4_3420 at 3810.924 kb on - strand, within Pf1N1B4_3420 at 3810.953 kb on - strand, within Pf1N1B4_3420 at 3810.953 kb on - strand, within Pf1N1B4_3420 at 3811.142 kb on + strand at 3811.143 kb on - strand at 3811.151 kb on + strand at 3811.152 kb on - strand at 3811.156 kb on + strand at 3811.157 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 3,806,983 + +0.4 3,806,984 - -1.5 3,806,984 - -0.2 3,806,984 - -0.1 3,806,999 + -1.0 3,807,000 - -0.1 3,807,026 + -0.7 3,807,026 + -0.3 3,807,027 - +1.0 3,807,027 - +1.6 3,807,027 - +1.0 3,807,085 + +0.4 3,807,085 + -0.3 3,807,097 + -0.6 3,807,098 - -0.6 3,807,098 - -0.6 3,807,189 + -0.6 3,807,307 + +2.6 3,807,308 - +0.4 3,807,749 - -0.0 3,807,894 + +1.2 3,807,894 + -1.8 3,807,894 + -0.0 3,807,903 + +0.1 3,807,903 + -0.2 3,807,903 + -0.6 3,807,903 + +0.4 3,807,904 - -1.2 3,807,904 - -0.8 3,807,987 + -0.4 3,808,065 + -0.0 3,808,103 + +0.6 3,808,104 - +0.2 3,808,197 + Pf1N1B4_3419 0.14 -0.2 3,808,335 + Pf1N1B4_3419 0.20 -1.4 3,808,335 + Pf1N1B4_3419 0.20 -0.2 3,808,335 + -0.6 3,808,335 + Pf1N1B4_3419 0.20 -0.4 3,808,335 + Pf1N1B4_3419 0.20 +0.5 3,808,335 + Pf1N1B4_3419 0.20 -0.2 3,808,336 - Pf1N1B4_3419 0.20 -0.2 3,808,336 - Pf1N1B4_3419 0.20 -0.5 3,808,336 - Pf1N1B4_3419 0.20 -0.6 3,808,336 - Pf1N1B4_3419 0.20 +1.0 3,808,336 - Pf1N1B4_3419 0.20 +0.4 3,808,394 + Pf1N1B4_3419 0.22 -0.4 3,808,395 - Pf1N1B4_3419 0.22 +0.4 3,808,496 + Pf1N1B4_3419 0.27 -1.1 3,808,496 + Pf1N1B4_3419 0.27 -1.3 3,808,496 + Pf1N1B4_3419 0.27 -0.2 3,808,496 + Pf1N1B4_3419 0.27 +1.0 3,808,497 - Pf1N1B4_3419 0.27 -0.5 3,808,497 - Pf1N1B4_3419 0.27 +0.4 3,808,523 + Pf1N1B4_3419 0.28 -0.6 3,808,523 + Pf1N1B4_3419 0.28 -0.0 3,808,565 + Pf1N1B4_3419 0.30 -0.8 3,808,577 + Pf1N1B4_3419 0.30 -0.2 3,808,633 - Pf1N1B4_3419 0.33 -0.0 3,808,807 + Pf1N1B4_3419 0.40 +1.0 3,808,829 + Pf1N1B4_3419 0.41 -0.6 3,808,830 - Pf1N1B4_3419 0.41 -0.0 3,808,843 - Pf1N1B4_3419 0.42 -0.5 3,808,843 - Pf1N1B4_3419 0.42 -0.6 3,808,919 + Pf1N1B4_3419 0.45 -0.3 3,808,919 + Pf1N1B4_3419 0.45 -0.6 3,808,920 - Pf1N1B4_3419 0.45 -1.8 3,809,028 + Pf1N1B4_3419 0.50 -0.9 3,809,028 + Pf1N1B4_3419 0.50 -1.8 3,809,106 + Pf1N1B4_3419 0.53 +1.4 3,809,107 - -0.0 3,809,107 - Pf1N1B4_3419 0.53 -0.0 3,809,187 - Pf1N1B4_3419 0.57 -2.6 3,809,228 + Pf1N1B4_3419 0.59 -0.2 3,809,228 + Pf1N1B4_3419 0.59 +0.6 3,809,228 + Pf1N1B4_3419 0.59 -0.2 3,809,228 + Pf1N1B4_3419 0.59 +0.5 3,809,228 + Pf1N1B4_3419 0.59 -1.3 3,809,229 - Pf1N1B4_3419 0.59 -1.3 3,809,229 - Pf1N1B4_3419 0.59 -1.8 3,809,229 - Pf1N1B4_3419 0.59 -0.3 3,809,270 - Pf1N1B4_3419 0.60 -0.8 3,809,273 + Pf1N1B4_3419 0.61 -0.0 3,809,385 + Pf1N1B4_3419 0.65 -1.2 3,809,573 + Pf1N1B4_3419 0.74 +0.4 3,809,574 - Pf1N1B4_3419 0.74 -0.3 3,809,574 - Pf1N1B4_3419 0.74 -0.0 3,809,625 - Pf1N1B4_3419 0.76 +1.0 3,809,644 - -1.0 3,809,684 + Pf1N1B4_3419 0.79 +0.2 3,809,684 + -0.0 3,809,684 + Pf1N1B4_3419 0.79 -1.3 3,809,684 + Pf1N1B4_3419 0.79 +1.0 3,809,684 + Pf1N1B4_3419 0.79 -1.2 3,809,684 + Pf1N1B4_3419 0.79 -0.0 3,809,685 - Pf1N1B4_3419 0.79 -0.0 3,809,685 - Pf1N1B4_3419 0.79 +0.7 3,809,685 - Pf1N1B4_3419 0.79 -2.0 3,809,687 + Pf1N1B4_3419 0.79 +0.4 3,809,714 + Pf1N1B4_3419 0.80 -0.0 3,809,715 - Pf1N1B4_3419 0.80 +0.3 3,809,718 + Pf1N1B4_3419 0.80 -1.1 3,809,718 + Pf1N1B4_3419 0.80 +0.9 3,809,718 + -0.6 3,809,719 - Pf1N1B4_3419 0.80 -0.7 3,809,719 - Pf1N1B4_3419 0.80 +0.2 3,809,719 - Pf1N1B4_3419 0.80 +1.0 3,809,738 + Pf1N1B4_3419 0.81 -0.5 3,809,738 + Pf1N1B4_3419 0.81 +0.2 3,809,738 + Pf1N1B4_3419 0.81 +0.2 3,809,738 + Pf1N1B4_3419 0.81 -0.0 3,809,739 - Pf1N1B4_3419 0.81 -1.6 3,809,751 - Pf1N1B4_3419 0.81 +0.2 3,809,801 + Pf1N1B4_3419 0.84 -2.2 3,809,802 - Pf1N1B4_3419 0.84 -2.2 3,809,802 - Pf1N1B4_3419 0.84 -0.4 3,809,802 - Pf1N1B4_3419 0.84 -1.8 3,809,925 + Pf1N1B4_3419 0.89 -0.3 3,809,925 + Pf1N1B4_3419 0.89 -0.0 3,809,925 + Pf1N1B4_3419 0.89 -1.0 3,809,925 + Pf1N1B4_3419 0.89 -1.6 3,809,925 + Pf1N1B4_3419 0.89 -0.3 3,809,926 - Pf1N1B4_3419 0.89 +0.4 3,809,926 - Pf1N1B4_3419 0.89 +0.1 3,809,926 - Pf1N1B4_3419 0.89 +1.0 3,809,927 + Pf1N1B4_3419 0.89 +0.4 3,809,927 + Pf1N1B4_3419 0.89 -1.3 3,809,927 + Pf1N1B4_3419 0.89 -0.2 3,809,927 + Pf1N1B4_3419 0.89 -2.3 3,809,927 + +1.0 3,809,927 + Pf1N1B4_3419 0.89 -1.6 3,809,927 + Pf1N1B4_3419 0.89 -0.2 3,809,927 + Pf1N1B4_3419 0.89 -2.0 3,809,927 + Pf1N1B4_3419 0.89 -2.3 3,809,927 + Pf1N1B4_3419 0.89 -1.0 3,809,927 + Pf1N1B4_3419 0.89 +0.8 3,809,927 + Pf1N1B4_3419 0.89 +0.3 3,809,928 - Pf1N1B4_3419 0.89 -1.3 3,809,928 - Pf1N1B4_3419 0.89 +0.6 3,809,928 - +1.0 3,809,928 - +2.0 3,809,928 - Pf1N1B4_3419 0.89 -1.0 3,809,928 - Pf1N1B4_3419 0.89 +2.4 3,809,928 - Pf1N1B4_3419 0.89 +0.8 3,809,928 - Pf1N1B4_3419 0.89 -1.6 3,809,928 - Pf1N1B4_3419 0.89 -1.7 3,809,929 + Pf1N1B4_3419 0.89 +0.1 3,809,929 + Pf1N1B4_3419 0.89 -1.2 3,809,929 + Pf1N1B4_3419 0.89 -1.2 3,809,930 - Pf1N1B4_3419 0.89 -1.0 3,809,930 - Pf1N1B4_3419 0.89 +1.4 3,810,058 + +0.3 3,810,195 - +1.8 3,810,243 - -1.8 3,810,308 - +1.3 3,810,308 - -1.0 3,810,308 - +0.7 3,810,522 - Pf1N1B4_3420 0.28 +0.6 3,810,534 - Pf1N1B4_3420 0.30 +2.0 3,810,564 + Pf1N1B4_3420 0.34 +0.3 3,810,565 - Pf1N1B4_3420 0.34 -1.3 3,810,657 - Pf1N1B4_3420 0.46 +1.6 3,810,715 + Pf1N1B4_3420 0.53 +1.2 3,810,820 + Pf1N1B4_3420 0.67 -1.6 3,810,820 + Pf1N1B4_3420 0.67 -1.0 3,810,820 + Pf1N1B4_3420 0.67 +2.0 3,810,821 - Pf1N1B4_3420 0.67 +1.7 3,810,821 - Pf1N1B4_3420 0.67 +1.0 3,810,821 - Pf1N1B4_3420 0.67 +1.2 3,810,821 - Pf1N1B4_3420 0.67 -0.3 3,810,821 - Pf1N1B4_3420 0.67 +0.2 3,810,923 + Pf1N1B4_3420 0.80 +1.8 3,810,924 - Pf1N1B4_3420 0.80 +0.2 3,810,953 - Pf1N1B4_3420 0.84 +0.6 3,810,953 - Pf1N1B4_3420 0.84 -1.3 3,811,142 + +1.0 3,811,143 - -0.0 3,811,151 + -1.3 3,811,152 - -0.3 3,811,156 + -0.6 3,811,157 - -0.8
Or see this region's nucleotide sequence