Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3308 and Pf1N1B4_3309 are separated by 129 nucleotides Pf1N1B4_3309 and Pf1N1B4_3310 are separated by 115 nucleotides
Pf1N1B4_3308 - Enoyl-CoA hydratase (EC 4.2.1.17), at 3,696,875 to 3,697,990
_3308
Pf1N1B4_3309 - Uracil-DNA glycosylase, family 1, at 3,698,120 to 3,698,812
_3309
Pf1N1B4_3310 - Ammonia monooxygenase, at 3,698,928 to 3,699,968
_3310
Position (kb)
3698
3699 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3697.641 kb on + strand, within Pf1N1B4_3308 at 3697.642 kb on - strand, within Pf1N1B4_3308 at 3697.642 kb on - strand, within Pf1N1B4_3308 at 3697.642 kb on - strand, within Pf1N1B4_3308 at 3697.674 kb on + strand, within Pf1N1B4_3308 at 3697.674 kb on + strand, within Pf1N1B4_3308 at 3697.779 kb on - strand, within Pf1N1B4_3308 at 3697.779 kb on - strand, within Pf1N1B4_3308 at 3697.779 kb on - strand, within Pf1N1B4_3308 at 3698.004 kb on - strand at 3698.016 kb on + strand at 3698.016 kb on + strand at 3698.017 kb on - strand at 3698.017 kb on - strand at 3698.017 kb on - strand at 3698.019 kb on + strand at 3698.020 kb on - strand at 3698.020 kb on - strand at 3698.032 kb on + strand at 3698.052 kb on - strand at 3698.115 kb on + strand at 3698.116 kb on - strand at 3698.116 kb on - strand at 3698.116 kb on - strand at 3698.116 kb on - strand at 3698.192 kb on + strand, within Pf1N1B4_3309 at 3698.192 kb on + strand, within Pf1N1B4_3309 at 3698.192 kb on + strand, within Pf1N1B4_3309 at 3698.192 kb on + strand at 3698.192 kb on + strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.193 kb on - strand, within Pf1N1B4_3309 at 3698.249 kb on + strand, within Pf1N1B4_3309 at 3698.250 kb on - strand, within Pf1N1B4_3309 at 3698.250 kb on - strand, within Pf1N1B4_3309 at 3698.250 kb on - strand, within Pf1N1B4_3309 at 3698.250 kb on - strand at 3698.250 kb on - strand, within Pf1N1B4_3309 at 3698.415 kb on - strand, within Pf1N1B4_3309 at 3698.463 kb on - strand, within Pf1N1B4_3309 at 3698.561 kb on + strand, within Pf1N1B4_3309 at 3698.562 kb on - strand, within Pf1N1B4_3309 at 3698.562 kb on - strand, within Pf1N1B4_3309 at 3698.613 kb on + strand, within Pf1N1B4_3309 at 3698.613 kb on + strand, within Pf1N1B4_3309 at 3698.614 kb on - strand, within Pf1N1B4_3309 at 3698.985 kb on + strand at 3698.985 kb on + strand at 3699.478 kb on + strand, within Pf1N1B4_3310 at 3699.479 kb on - strand, within Pf1N1B4_3310 at 3699.681 kb on - strand, within Pf1N1B4_3310
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 3,697,641 + Pf1N1B4_3308 0.69 -1.1 3,697,642 - Pf1N1B4_3308 0.69 -1.1 3,697,642 - Pf1N1B4_3308 0.69 +1.2 3,697,642 - Pf1N1B4_3308 0.69 +0.6 3,697,674 + Pf1N1B4_3308 0.72 -1.3 3,697,674 + Pf1N1B4_3308 0.72 -1.0 3,697,779 - Pf1N1B4_3308 0.81 +1.0 3,697,779 - Pf1N1B4_3308 0.81 +0.5 3,697,779 - Pf1N1B4_3308 0.81 +0.4 3,698,004 - -1.1 3,698,016 + +0.0 3,698,016 + -2.1 3,698,017 - -0.6 3,698,017 - +0.2 3,698,017 - -0.6 3,698,019 + +0.0 3,698,020 - -1.3 3,698,020 - -1.2 3,698,032 + +0.5 3,698,052 - -0.6 3,698,115 + -3.0 3,698,116 - +0.4 3,698,116 - -2.3 3,698,116 - +0.6 3,698,116 - -1.8 3,698,192 + Pf1N1B4_3309 0.10 -0.8 3,698,192 + Pf1N1B4_3309 0.10 -2.4 3,698,192 + Pf1N1B4_3309 0.10 -2.0 3,698,192 + -0.6 3,698,192 + Pf1N1B4_3309 0.10 -1.3 3,698,193 - Pf1N1B4_3309 0.11 +0.5 3,698,193 - Pf1N1B4_3309 0.11 -0.3 3,698,193 - Pf1N1B4_3309 0.11 +0.0 3,698,193 - Pf1N1B4_3309 0.11 -1.0 3,698,193 - Pf1N1B4_3309 0.11 +0.8 3,698,193 - Pf1N1B4_3309 0.11 -0.2 3,698,193 - Pf1N1B4_3309 0.11 -1.0 3,698,193 - Pf1N1B4_3309 0.11 -0.3 3,698,249 + Pf1N1B4_3309 0.19 -0.6 3,698,250 - Pf1N1B4_3309 0.19 +1.0 3,698,250 - Pf1N1B4_3309 0.19 -0.3 3,698,250 - Pf1N1B4_3309 0.19 -0.8 3,698,250 - +0.3 3,698,250 - Pf1N1B4_3309 0.19 -1.0 3,698,415 - Pf1N1B4_3309 0.43 -1.3 3,698,463 - Pf1N1B4_3309 0.49 -1.3 3,698,561 + Pf1N1B4_3309 0.64 +0.3 3,698,562 - Pf1N1B4_3309 0.64 -1.3 3,698,562 - Pf1N1B4_3309 0.64 -1.8 3,698,613 + Pf1N1B4_3309 0.71 -1.8 3,698,613 + Pf1N1B4_3309 0.71 -1.6 3,698,614 - Pf1N1B4_3309 0.71 +0.0 3,698,985 + +0.4 3,698,985 + +0.5 3,699,478 + Pf1N1B4_3310 0.53 -2.0 3,699,479 - Pf1N1B4_3310 0.53 +1.3 3,699,681 - Pf1N1B4_3310 0.72 +0.0
Or see this region's nucleotide sequence