Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3301 and Pf1N1B4_3302 are separated by 305 nucleotides Pf1N1B4_3302 and Pf1N1B4_3303 are separated by 11 nucleotides Pf1N1B4_3303 and Pf1N1B4_3304 are separated by 12 nucleotides Pf1N1B4_3304 and Pf1N1B4_3305 overlap by 4 nucleotides
Pf1N1B4_3301 - Urea carboxylase (EC 6.3.4.6), at 3,687,408 to 3,691,049
_3301
Pf1N1B4_3302 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,691,355 to 3,691,996
_3302
Pf1N1B4_3303 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,692,008 to 3,692,733
_3303
Pf1N1B4_3304 - Urea carboxylase-related ABC transporter, ATPase protein, at 3,692,746 to 3,693,534
_3304
Pf1N1B4_3305 - Urea carboxylase-related ABC transporter, permease protein, at 3,693,531 to 3,694,346
_3305
Position (kb)
3692
3693 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3691.284 kb on - strand at 3691.284 kb on - strand at 3691.284 kb on - strand at 3691.392 kb on + strand at 3691.392 kb on + strand at 3691.393 kb on - strand at 3691.470 kb on + strand at 3691.470 kb on + strand, within Pf1N1B4_3302 at 3691.471 kb on - strand, within Pf1N1B4_3302 at 3691.471 kb on - strand, within Pf1N1B4_3302 at 3691.471 kb on - strand, within Pf1N1B4_3302 at 3691.605 kb on + strand, within Pf1N1B4_3302 at 3691.605 kb on + strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.629 kb on + strand, within Pf1N1B4_3302 at 3691.749 kb on + strand, within Pf1N1B4_3302 at 3691.750 kb on - strand, within Pf1N1B4_3302 at 3691.773 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.813 kb on - strand, within Pf1N1B4_3302 at 3691.840 kb on - strand, within Pf1N1B4_3302 at 3692.195 kb on + strand, within Pf1N1B4_3303 at 3692.207 kb on + strand, within Pf1N1B4_3303 at 3692.208 kb on - strand, within Pf1N1B4_3303 at 3692.208 kb on - strand, within Pf1N1B4_3303 at 3692.208 kb on - strand at 3692.322 kb on + strand, within Pf1N1B4_3303 at 3692.747 kb on + strand at 3692.837 kb on + strand, within Pf1N1B4_3304 at 3692.837 kb on + strand, within Pf1N1B4_3304 at 3692.838 kb on - strand at 3692.838 kb on - strand, within Pf1N1B4_3304 at 3692.838 kb on - strand, within Pf1N1B4_3304 at 3692.855 kb on + strand at 3692.856 kb on - strand, within Pf1N1B4_3304 at 3692.856 kb on - strand, within Pf1N1B4_3304 at 3693.370 kb on - strand, within Pf1N1B4_3304 at 3693.388 kb on + strand, within Pf1N1B4_3304 at 3693.389 kb on - strand, within Pf1N1B4_3304 at 3693.391 kb on + strand, within Pf1N1B4_3304 at 3693.392 kb on - strand at 3693.485 kb on - strand at 3693.497 kb on + strand at 3693.498 kb on - strand at 3693.498 kb on - strand at 3693.498 kb on - strand at 3693.550 kb on + strand at 3693.550 kb on + strand at 3693.550 kb on + strand at 3693.551 kb on - strand at 3693.704 kb on + strand, within Pf1N1B4_3305 at 3693.704 kb on + strand, within Pf1N1B4_3305
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 3,691,284 - +0.6 3,691,284 - +0.4 3,691,284 - +2.0 3,691,392 + -0.6 3,691,392 + -0.6 3,691,393 - +0.1 3,691,470 + -1.0 3,691,470 + Pf1N1B4_3302 0.18 -1.3 3,691,471 - Pf1N1B4_3302 0.18 -1.0 3,691,471 - Pf1N1B4_3302 0.18 +0.0 3,691,471 - Pf1N1B4_3302 0.18 +0.4 3,691,605 + Pf1N1B4_3302 0.39 +0.0 3,691,605 + Pf1N1B4_3302 0.39 -2.3 3,691,606 - Pf1N1B4_3302 0.39 +0.3 3,691,606 - Pf1N1B4_3302 0.39 +0.4 3,691,606 - Pf1N1B4_3302 0.39 +0.0 3,691,606 - Pf1N1B4_3302 0.39 -0.5 3,691,606 - +0.0 3,691,606 - Pf1N1B4_3302 0.39 +0.6 3,691,606 - Pf1N1B4_3302 0.39 -1.8 3,691,629 + Pf1N1B4_3302 0.43 -1.3 3,691,749 + Pf1N1B4_3302 0.61 -2.0 3,691,750 - Pf1N1B4_3302 0.62 +1.3 3,691,773 + Pf1N1B4_3302 0.65 -0.2 3,691,805 + Pf1N1B4_3302 0.70 +0.1 3,691,805 + Pf1N1B4_3302 0.70 -1.0 3,691,805 + -0.6 3,691,805 + Pf1N1B4_3302 0.70 +0.7 3,691,805 + Pf1N1B4_3302 0.70 +1.4 3,691,806 - Pf1N1B4_3302 0.70 +0.0 3,691,806 - Pf1N1B4_3302 0.70 +0.3 3,691,806 - Pf1N1B4_3302 0.70 -1.0 3,691,806 - Pf1N1B4_3302 0.70 +0.8 3,691,813 - Pf1N1B4_3302 0.71 +1.0 3,691,840 - Pf1N1B4_3302 0.76 -1.8 3,692,195 + Pf1N1B4_3303 0.26 +0.2 3,692,207 + Pf1N1B4_3303 0.27 -0.7 3,692,208 - Pf1N1B4_3303 0.28 -1.6 3,692,208 - Pf1N1B4_3303 0.28 -1.3 3,692,208 - -1.0 3,692,322 + Pf1N1B4_3303 0.43 -0.3 3,692,747 + -0.6 3,692,837 + Pf1N1B4_3304 0.12 +1.2 3,692,837 + Pf1N1B4_3304 0.12 -0.7 3,692,838 - +1.0 3,692,838 - Pf1N1B4_3304 0.12 -1.8 3,692,838 - Pf1N1B4_3304 0.12 +0.2 3,692,855 + +0.0 3,692,856 - Pf1N1B4_3304 0.14 +0.0 3,692,856 - Pf1N1B4_3304 0.14 +0.1 3,693,370 - Pf1N1B4_3304 0.79 +0.9 3,693,388 + Pf1N1B4_3304 0.81 -0.4 3,693,389 - Pf1N1B4_3304 0.81 +0.4 3,693,391 + Pf1N1B4_3304 0.82 +0.2 3,693,392 - +2.6 3,693,485 - +1.0 3,693,497 + +1.0 3,693,498 - -0.6 3,693,498 - +0.3 3,693,498 - -2.3 3,693,550 + +1.0 3,693,550 + -0.1 3,693,550 + +1.2 3,693,551 - -2.1 3,693,704 + Pf1N1B4_3305 0.21 -2.0 3,693,704 + Pf1N1B4_3305 0.21 +0.0
Or see this region's nucleotide sequence