Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3303

Experiment: nitrate 100 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3301 and Pf1N1B4_3302 are separated by 305 nucleotidesPf1N1B4_3302 and Pf1N1B4_3303 are separated by 11 nucleotidesPf1N1B4_3303 and Pf1N1B4_3304 are separated by 12 nucleotidesPf1N1B4_3304 and Pf1N1B4_3305 overlap by 4 nucleotides Pf1N1B4_3301 - Urea carboxylase (EC 6.3.4.6), at 3,687,408 to 3,691,049 _3301 Pf1N1B4_3302 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,691,355 to 3,691,996 _3302 Pf1N1B4_3303 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,692,008 to 3,692,733 _3303 Pf1N1B4_3304 - Urea carboxylase-related ABC transporter, ATPase protein, at 3,692,746 to 3,693,534 _3304 Pf1N1B4_3305 - Urea carboxylase-related ABC transporter, permease protein, at 3,693,531 to 3,694,346 _3305 Position (kb) 3692 3693Strain fitness (log2 ratio) -2 -1 0 1 2at 3691.284 kb on - strandat 3691.284 kb on - strandat 3691.284 kb on - strandat 3691.392 kb on + strandat 3691.392 kb on + strandat 3691.393 kb on - strandat 3691.470 kb on + strandat 3691.470 kb on + strand, within Pf1N1B4_3302at 3691.471 kb on - strand, within Pf1N1B4_3302at 3691.471 kb on - strand, within Pf1N1B4_3302at 3691.471 kb on - strand, within Pf1N1B4_3302at 3691.605 kb on + strand, within Pf1N1B4_3302at 3691.605 kb on + strand, within Pf1N1B4_3302at 3691.606 kb on - strand, within Pf1N1B4_3302at 3691.606 kb on - strand, within Pf1N1B4_3302at 3691.606 kb on - strand, within Pf1N1B4_3302at 3691.606 kb on - strand, within Pf1N1B4_3302at 3691.606 kb on - strandat 3691.606 kb on - strand, within Pf1N1B4_3302at 3691.606 kb on - strand, within Pf1N1B4_3302at 3691.629 kb on + strand, within Pf1N1B4_3302at 3691.749 kb on + strand, within Pf1N1B4_3302at 3691.750 kb on - strand, within Pf1N1B4_3302at 3691.773 kb on + strand, within Pf1N1B4_3302at 3691.805 kb on + strand, within Pf1N1B4_3302at 3691.805 kb on + strand, within Pf1N1B4_3302at 3691.805 kb on + strandat 3691.805 kb on + strand, within Pf1N1B4_3302at 3691.805 kb on + strand, within Pf1N1B4_3302at 3691.806 kb on - strand, within Pf1N1B4_3302at 3691.806 kb on - strand, within Pf1N1B4_3302at 3691.806 kb on - strand, within Pf1N1B4_3302at 3691.806 kb on - strand, within Pf1N1B4_3302at 3691.813 kb on - strand, within Pf1N1B4_3302at 3691.840 kb on - strand, within Pf1N1B4_3302at 3692.195 kb on + strand, within Pf1N1B4_3303at 3692.207 kb on + strand, within Pf1N1B4_3303at 3692.208 kb on - strand, within Pf1N1B4_3303at 3692.208 kb on - strand, within Pf1N1B4_3303at 3692.208 kb on - strandat 3692.322 kb on + strand, within Pf1N1B4_3303at 3692.747 kb on + strandat 3692.837 kb on + strand, within Pf1N1B4_3304at 3692.837 kb on + strand, within Pf1N1B4_3304at 3692.838 kb on - strandat 3692.838 kb on - strand, within Pf1N1B4_3304at 3692.838 kb on - strand, within Pf1N1B4_3304at 3692.855 kb on + strandat 3692.856 kb on - strand, within Pf1N1B4_3304at 3692.856 kb on - strand, within Pf1N1B4_3304at 3693.370 kb on - strand, within Pf1N1B4_3304at 3693.388 kb on + strand, within Pf1N1B4_3304at 3693.389 kb on - strand, within Pf1N1B4_3304at 3693.391 kb on + strand, within Pf1N1B4_3304at 3693.392 kb on - strandat 3693.485 kb on - strandat 3693.497 kb on + strandat 3693.498 kb on - strandat 3693.498 kb on - strandat 3693.498 kb on - strandat 3693.550 kb on + strandat 3693.550 kb on + strandat 3693.550 kb on + strandat 3693.551 kb on - strandat 3693.704 kb on + strand, within Pf1N1B4_3305at 3693.704 kb on + strand, within Pf1N1B4_3305

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 100 mM
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3,691,284 - +0.6
3,691,284 - +0.4
3,691,284 - +2.0
3,691,392 + -0.6
3,691,392 + -0.6
3,691,393 - +0.1
3,691,470 + -1.0
3,691,470 + Pf1N1B4_3302 0.18 -1.3
3,691,471 - Pf1N1B4_3302 0.18 -1.0
3,691,471 - Pf1N1B4_3302 0.18 +0.0
3,691,471 - Pf1N1B4_3302 0.18 +0.4
3,691,605 + Pf1N1B4_3302 0.39 +0.0
3,691,605 + Pf1N1B4_3302 0.39 -2.3
3,691,606 - Pf1N1B4_3302 0.39 +0.3
3,691,606 - Pf1N1B4_3302 0.39 +0.4
3,691,606 - Pf1N1B4_3302 0.39 +0.0
3,691,606 - Pf1N1B4_3302 0.39 -0.5
3,691,606 - +0.0
3,691,606 - Pf1N1B4_3302 0.39 +0.6
3,691,606 - Pf1N1B4_3302 0.39 -1.8
3,691,629 + Pf1N1B4_3302 0.43 -1.3
3,691,749 + Pf1N1B4_3302 0.61 -2.0
3,691,750 - Pf1N1B4_3302 0.62 +1.3
3,691,773 + Pf1N1B4_3302 0.65 -0.2
3,691,805 + Pf1N1B4_3302 0.70 +0.1
3,691,805 + Pf1N1B4_3302 0.70 -1.0
3,691,805 + -0.6
3,691,805 + Pf1N1B4_3302 0.70 +0.7
3,691,805 + Pf1N1B4_3302 0.70 +1.4
3,691,806 - Pf1N1B4_3302 0.70 +0.0
3,691,806 - Pf1N1B4_3302 0.70 +0.3
3,691,806 - Pf1N1B4_3302 0.70 -1.0
3,691,806 - Pf1N1B4_3302 0.70 +0.8
3,691,813 - Pf1N1B4_3302 0.71 +1.0
3,691,840 - Pf1N1B4_3302 0.76 -1.8
3,692,195 + Pf1N1B4_3303 0.26 +0.2
3,692,207 + Pf1N1B4_3303 0.27 -0.7
3,692,208 - Pf1N1B4_3303 0.28 -1.6
3,692,208 - Pf1N1B4_3303 0.28 -1.3
3,692,208 - -1.0
3,692,322 + Pf1N1B4_3303 0.43 -0.3
3,692,747 + -0.6
3,692,837 + Pf1N1B4_3304 0.12 +1.2
3,692,837 + Pf1N1B4_3304 0.12 -0.7
3,692,838 - +1.0
3,692,838 - Pf1N1B4_3304 0.12 -1.8
3,692,838 - Pf1N1B4_3304 0.12 +0.2
3,692,855 + +0.0
3,692,856 - Pf1N1B4_3304 0.14 +0.0
3,692,856 - Pf1N1B4_3304 0.14 +0.1
3,693,370 - Pf1N1B4_3304 0.79 +0.9
3,693,388 + Pf1N1B4_3304 0.81 -0.4
3,693,389 - Pf1N1B4_3304 0.81 +0.4
3,693,391 + Pf1N1B4_3304 0.82 +0.2
3,693,392 - +2.6
3,693,485 - +1.0
3,693,497 + +1.0
3,693,498 - -0.6
3,693,498 - +0.3
3,693,498 - -2.3
3,693,550 + +1.0
3,693,550 + -0.1
3,693,550 + +1.2
3,693,551 - -2.1
3,693,704 + Pf1N1B4_3305 0.21 -2.0
3,693,704 + Pf1N1B4_3305 0.21 +0.0

Or see this region's nucleotide sequence