Experiment: nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2931 and Pf1N1B4_2932 are separated by 8 nucleotides Pf1N1B4_2932 and Pf1N1B4_2933 are separated by 86 nucleotides
Pf1N1B4_2931 - Cell division inhibitor, at 3,287,679 to 3,288,587
_2931
Pf1N1B4_2932 - Ferrochelatase, protoheme ferro-lyase (EC 4.99.1.1), at 3,288,596 to 3,289,618
_2932
Pf1N1B4_2933 - Uracil permease, at 3,289,705 to 3,290,979
_2933
Position (kb)
3288
3289
3290 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3287.612 kb on + strand at 3287.612 kb on + strand at 3287.612 kb on + strand at 3287.632 kb on - strand at 3287.632 kb on - strand at 3287.637 kb on + strand at 3287.642 kb on + strand at 3287.686 kb on + strand at 3287.686 kb on + strand at 3287.687 kb on - strand at 3287.687 kb on - strand at 3287.687 kb on - strand at 3287.687 kb on - strand at 3287.687 kb on - strand at 3287.687 kb on - strand at 3287.687 kb on - strand at 3287.687 kb on - strand at 3287.702 kb on + strand at 3287.713 kb on + strand at 3287.864 kb on + strand, within Pf1N1B4_2931 at 3287.864 kb on + strand, within Pf1N1B4_2931 at 3287.864 kb on + strand, within Pf1N1B4_2931 at 3287.865 kb on - strand, within Pf1N1B4_2931 at 3287.865 kb on - strand, within Pf1N1B4_2931 at 3287.865 kb on - strand, within Pf1N1B4_2931 at 3288.028 kb on - strand, within Pf1N1B4_2931 at 3288.278 kb on + strand, within Pf1N1B4_2931 at 3288.278 kb on + strand, within Pf1N1B4_2931 at 3288.279 kb on - strand, within Pf1N1B4_2931 at 3288.279 kb on - strand, within Pf1N1B4_2931 at 3288.279 kb on - strand, within Pf1N1B4_2931 at 3288.292 kb on + strand, within Pf1N1B4_2931 at 3288.339 kb on + strand, within Pf1N1B4_2931 at 3288.339 kb on + strand, within Pf1N1B4_2931 at 3288.339 kb on + strand, within Pf1N1B4_2931 at 3288.339 kb on + strand, within Pf1N1B4_2931 at 3288.339 kb on + strand, within Pf1N1B4_2931 at 3288.339 kb on + strand, within Pf1N1B4_2931 at 3288.339 kb on + strand, within Pf1N1B4_2931 at 3288.339 kb on + strand, within Pf1N1B4_2931 at 3288.339 kb on + strand, within Pf1N1B4_2931 at 3288.339 kb on + strand, within Pf1N1B4_2931 at 3288.340 kb on - strand, within Pf1N1B4_2931 at 3288.340 kb on - strand, within Pf1N1B4_2931 at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.341 kb on + strand at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.341 kb on + strand, within Pf1N1B4_2931 at 3288.342 kb on - strand, within Pf1N1B4_2931 at 3288.342 kb on - strand at 3288.342 kb on - strand, within Pf1N1B4_2931 at 3288.342 kb on - strand, within Pf1N1B4_2931 at 3288.342 kb on - strand, within Pf1N1B4_2931 at 3288.585 kb on + strand at 3289.616 kb on + strand at 3289.706 kb on + strand at 3289.706 kb on + strand at 3289.706 kb on + strand at 3289.706 kb on + strand at 3289.707 kb on - strand at 3289.707 kb on - strand at 3289.707 kb on - strand at 3289.707 kb on - strand at 3289.707 kb on - strand at 3289.906 kb on + strand, within Pf1N1B4_2933 at 3289.907 kb on - strand, within Pf1N1B4_2933 at 3290.084 kb on + strand at 3290.084 kb on + strand, within Pf1N1B4_2933 at 3290.084 kb on + strand, within Pf1N1B4_2933 at 3290.084 kb on + strand, within Pf1N1B4_2933 at 3290.084 kb on + strand, within Pf1N1B4_2933 at 3290.084 kb on + strand, within Pf1N1B4_2933 at 3290.084 kb on + strand, within Pf1N1B4_2933 at 3290.085 kb on - strand, within Pf1N1B4_2933 at 3290.085 kb on - strand, within Pf1N1B4_2933 at 3290.085 kb on - strand, within Pf1N1B4_2933 at 3290.085 kb on - strand, within Pf1N1B4_2933 at 3290.085 kb on - strand at 3290.085 kb on - strand, within Pf1N1B4_2933 at 3290.085 kb on - strand, within Pf1N1B4_2933 at 3290.085 kb on - strand, within Pf1N1B4_2933 at 3290.085 kb on - strand, within Pf1N1B4_2933 at 3290.085 kb on - strand, within Pf1N1B4_2933 at 3290.085 kb on - strand, within Pf1N1B4_2933 at 3290.124 kb on - strand, within Pf1N1B4_2933 at 3290.124 kb on - strand, within Pf1N1B4_2933 at 3290.124 kb on - strand, within Pf1N1B4_2933 at 3290.124 kb on - strand, within Pf1N1B4_2933 at 3290.216 kb on + strand, within Pf1N1B4_2933 at 3290.217 kb on - strand, within Pf1N1B4_2933 at 3290.394 kb on - strand, within Pf1N1B4_2933 at 3290.394 kb on - strand, within Pf1N1B4_2933 at 3290.419 kb on + strand, within Pf1N1B4_2933 at 3290.419 kb on + strand, within Pf1N1B4_2933 at 3290.420 kb on - strand, within Pf1N1B4_2933 at 3290.420 kb on - strand, within Pf1N1B4_2933
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 100 mM remove 3,287,612 + -0.7 3,287,612 + -1.1 3,287,612 + -0.8 3,287,632 - +0.6 3,287,632 - -0.9 3,287,637 + -0.8 3,287,642 + -0.6 3,287,686 + -0.2 3,287,686 + -0.9 3,287,687 - -0.8 3,287,687 - -0.1 3,287,687 - +0.8 3,287,687 - +1.2 3,287,687 - +0.4 3,287,687 - -0.4 3,287,687 - +0.8 3,287,687 - -2.3 3,287,702 + -0.4 3,287,713 + -0.4 3,287,864 + Pf1N1B4_2931 0.20 -1.1 3,287,864 + Pf1N1B4_2931 0.20 +0.4 3,287,864 + Pf1N1B4_2931 0.20 -2.1 3,287,865 - Pf1N1B4_2931 0.20 -1.0 3,287,865 - Pf1N1B4_2931 0.20 -0.3 3,287,865 - Pf1N1B4_2931 0.20 +0.4 3,288,028 - Pf1N1B4_2931 0.38 -1.1 3,288,278 + Pf1N1B4_2931 0.66 -1.4 3,288,278 + Pf1N1B4_2931 0.66 +1.2 3,288,279 - Pf1N1B4_2931 0.66 +1.2 3,288,279 - Pf1N1B4_2931 0.66 -2.9 3,288,279 - Pf1N1B4_2931 0.66 +0.6 3,288,292 + Pf1N1B4_2931 0.67 -1.1 3,288,339 + Pf1N1B4_2931 0.73 -1.8 3,288,339 + Pf1N1B4_2931 0.73 -0.4 3,288,339 + Pf1N1B4_2931 0.73 -0.4 3,288,339 + Pf1N1B4_2931 0.73 -0.8 3,288,339 + Pf1N1B4_2931 0.73 -1.4 3,288,339 + Pf1N1B4_2931 0.73 -0.8 3,288,339 + Pf1N1B4_2931 0.73 +0.2 3,288,339 + Pf1N1B4_2931 0.73 -1.6 3,288,339 + Pf1N1B4_2931 0.73 +0.5 3,288,339 + Pf1N1B4_2931 0.73 +0.6 3,288,340 - Pf1N1B4_2931 0.73 +0.2 3,288,340 - Pf1N1B4_2931 0.73 -0.7 3,288,341 + Pf1N1B4_2931 0.73 +0.3 3,288,341 + +0.8 3,288,341 + Pf1N1B4_2931 0.73 +0.6 3,288,341 + Pf1N1B4_2931 0.73 -1.1 3,288,341 + Pf1N1B4_2931 0.73 +0.6 3,288,341 + Pf1N1B4_2931 0.73 +0.4 3,288,341 + Pf1N1B4_2931 0.73 +2.8 3,288,341 + Pf1N1B4_2931 0.73 +2.2 3,288,341 + Pf1N1B4_2931 0.73 +1.2 3,288,341 + Pf1N1B4_2931 0.73 +0.4 3,288,341 + Pf1N1B4_2931 0.73 -0.5 3,288,341 + Pf1N1B4_2931 0.73 -1.4 3,288,341 + Pf1N1B4_2931 0.73 -1.6 3,288,341 + Pf1N1B4_2931 0.73 -1.1 3,288,342 - Pf1N1B4_2931 0.73 +0.2 3,288,342 - +0.2 3,288,342 - Pf1N1B4_2931 0.73 -0.8 3,288,342 - Pf1N1B4_2931 0.73 +0.8 3,288,342 - Pf1N1B4_2931 0.73 -0.8 3,288,585 + +1.0 3,289,616 + +0.2 3,289,706 + -0.8 3,289,706 + -0.6 3,289,706 + +3.2 3,289,706 + +0.8 3,289,707 - +0.3 3,289,707 - -0.8 3,289,707 - -1.1 3,289,707 - +0.6 3,289,707 - -0.6 3,289,906 + Pf1N1B4_2933 0.16 +0.3 3,289,907 - Pf1N1B4_2933 0.16 -0.7 3,290,084 + +1.2 3,290,084 + Pf1N1B4_2933 0.30 -1.0 3,290,084 + Pf1N1B4_2933 0.30 +1.2 3,290,084 + Pf1N1B4_2933 0.30 +0.5 3,290,084 + Pf1N1B4_2933 0.30 +1.2 3,290,084 + Pf1N1B4_2933 0.30 +1.5 3,290,084 + Pf1N1B4_2933 0.30 -2.0 3,290,085 - Pf1N1B4_2933 0.30 +0.9 3,290,085 - Pf1N1B4_2933 0.30 -1.5 3,290,085 - Pf1N1B4_2933 0.30 +0.4 3,290,085 - Pf1N1B4_2933 0.30 -1.1 3,290,085 - -1.1 3,290,085 - Pf1N1B4_2933 0.30 -0.4 3,290,085 - Pf1N1B4_2933 0.30 -1.3 3,290,085 - Pf1N1B4_2933 0.30 +0.2 3,290,085 - Pf1N1B4_2933 0.30 +0.2 3,290,085 - Pf1N1B4_2933 0.30 -1.4 3,290,085 - Pf1N1B4_2933 0.30 +0.9 3,290,124 - Pf1N1B4_2933 0.33 -0.6 3,290,124 - Pf1N1B4_2933 0.33 +1.6 3,290,124 - Pf1N1B4_2933 0.33 +0.5 3,290,124 - Pf1N1B4_2933 0.33 -0.6 3,290,216 + Pf1N1B4_2933 0.40 -1.4 3,290,217 - Pf1N1B4_2933 0.40 -1.6 3,290,394 - Pf1N1B4_2933 0.54 -0.4 3,290,394 - Pf1N1B4_2933 0.54 +0.4 3,290,419 + Pf1N1B4_2933 0.56 +1.0 3,290,419 + Pf1N1B4_2933 0.56 -1.1 3,290,420 - Pf1N1B4_2933 0.56 -2.0 3,290,420 - Pf1N1B4_2933 0.56 +1.6
Or see this region's nucleotide sequence